Accession ID: MIRT000164 [miRNA, hsa-miR-21 :: WFS1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-21 LinkOut: [miRBase ]
Synonyms MIRN21, hsa-mir-21, miR-21, miRNA21, MIR21
Description Homo sapiens miR-21 stem-loop
Comment Mourelatos et al. named this sequence miR-21 precursor-17 and also reported the exact reverse complement of this predicted stem-loop sequence and erroneously assigned the name miR-104 .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-21-3p
Mature Sequence 46| CAACACCAGUCGAUGGGCUGU |66
Evidence Experimental
Experiments Cloned
Putative hsa-miR-21-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-21-5p
Mature Sequence 8| UAGCUUAUCAGACUGAUGUUGA |29
Evidence Experimental
Experiments Cloned
Putative hsa-miR-21-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol WFS1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms FLJ51211, WFRS, WFS, WOLFRAMIN
Description Wolfram syndrome 1 (wolframin)
Transcript NM_001145853   LinkOut: [ RefSeq ]
Other Transcripts NM_006005   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on WFS1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of WFS1
(miRNA target sites are highlighted)		 
>WFS1|NM_001145853|3'UTR
   1 TGAGGATGGTCCGCCACGAGGAGCTTCCAGTGCATGTTGCCATGAGGCCTTTCCCCAGTGTGGCCCCAGCCCGACAGGCA
  81 TGCACCAGTGCCGCCTGTGCCCACGTGTGCAGACTGTGGCTGCAGAGACCTTGCGACCATGTGTAGATTGCGTGGACCCC
 161 GACAAAGGGAAGGCTGCTGTGTAGCTCTGTCCACTCTGAATACCAAGTGTGTTGGGAATTGCATGCCATCTCCACCCTGA
 241 GCCTGACCTTTCTGAGTGACATGGGTGTGCCAGGCTAGACTAGGAGGTTCCGGTGTCTGGAAAAGCACTTTACAGATGAG
 321 ATTCCCTCTCCTCCCCCACCTTCAAGCACCCTGTTCCCTCTTTCTTTCTTTTGTGTTGGATTTGTTTAAAAACCAAATAA
 401 GCATCTGTGTAACCTCCACAGTAGCATTTCTTATTTGTTTGGTCACTGCTACACCTTAGCAGCTCTTCCCCTTTCCTGGG
 481 GGATGTGCACGGCAGCTTGAGCCTGTCACGTGGTCAAGGCCCGGCCCCATCAGAGGCTGGGGGAGGCGGCACATTGGCAG
 561 TGTGTCACACTGAGCTGGGCACCACAGGCTGCCTCATGACCCTCCTGTCCAGCAGGTAGTGGGTGAATGTGTGAAGGTCT
 641 TGCCTGAATCCATCAGGACTTGGGAAACAGAGAACCCTGTGGGGGCGGCTGTGGGGGAGGTCCCTGCCAGTGTTTAGAAG
 721 AGCCTGACTGTGTTCAGTGCCTTGGAGCAGAAAGCCAGGGTCCTGAGTGGCTGAAATAAAAGCCTCTGGTGGAACCTGCA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' ugucGGGUAGCUGACCACAAc 5'
              ::| |:  :| ||||| 
Target 5' ctctTTCTTTCTTTTGTGTTg 3'
 		358 - 378 113.00 -9.70
2 
miRNA  3' ugUCGGGUAGCUGACCACAAc 5'
            ||||   :|||| ||||| 
Target 5' agAGCC---TGACT-GTGTTc 3'
 		719 - 735 112.00 -13.50
3 
miRNA  3' ugUCGGGUAGCUGACCACaac 5'
            ||||:     ||||||   
Target 5' aaAGCCT-----CTGGTGgaa 3'
 		779 - 794 110.00 -12.42
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-21 :: WFS1    [ Functional MTI ]
Validation Method Quantitative proteomic approach
Conditions MCF-7
Location of target site 3'UTR
Tools used in this research miRanda, PicTar, TargetScan
Original Description (Extracted from the article) ... none ...

- Yang, Y. Chaerkady, R. Beer, M. A. Mendell, et al., 2009, Proteomics.
Article - Yang, Y. Chaerkady, R. Beer, M. A. Mendell, et al.
- Proteomics, 2009
MicroRNA (miRNA) play essential roles in biological processes ranging from cellular proliferation to apoptosis. Recently, miRNA have also been implicated in a number of diseases including cancers. However, the targets of most miRNA remain unknown. The majority of reports describing identification of miRNA targets are based on computational approaches or detection of altered mRNA levels despite the fact that most miRNA are thought to regulate their targets primarily at the level of translational inhibition in animals. miR-21 is a miRNA with oncogenic activity that is involved in various cancer-related processes such as invasion and migration. Given the importance of miR-21 in tumorigenesis, we employed a quantitative proteomic strategy to systematically identify potential targets of miR-21. By knocking down the expression of endogenous miR-21 in MCF-7 breast cancer cells, we observed an increase in the abundance of 58 proteins, implying that they could be potential targets of miR-21. Validation of 12 of these candidate targets in luciferase assays showed that 6 of them were likely direct targets of miR-21. Importantly, the mRNA of the majority of the candidate targets tested did not show a concomitant increase in abundance. Overall, our results demonstrate that miR-21 affects the expression of many of its targets through translational inhibition and highlights the utility of proteomic approaches for identifying miRNA targets.
LinkOut: [PMID: 19253296]