Accession ID: MIRT000320 [miRNA, hsa-miR-141 :: DLX5, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-141 LinkOut: [miRBase ]
Description Homo sapiens miR-141 stem-loop
Comment This miRNA sequence was predicted based on homology to a verified miRNA from mouse .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-141-3p
Mature Sequence 59| UAACACUGUCUGGUAAAGAUGG |80
Evidence Experimental
Experiments Cloned
Putative hsa-miR-141-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-141-5p
Mature Sequence 17| CAUCUUCCAGUACAGUGUUGGA |38
Evidence Experimental
Experiments Cloned
Putative hsa-miR-141-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol DLX5 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms -
Description distal-less homeobox 5
Transcript NM_005221   LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on DLX5 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of DLX5
(miRNA target sites are highlighted)		 
>DLX5|NM_005221|3'UTR
   1 TAGATGGGCTGCTCTCTCTTACTCTCTTTTTTGGGACTACTGTGTTTTGCTGTTCTAGAAAATCATAAAGAAAGGAATTC
  81 ATATGGGGAAGTTCGGAAAACTGAAAAAGATTCATGTGTAAAGCTTTTTTTTGCATGTAAGTTATTGCATTTCAAAAGAC
 161 CCCCCCTTTTTTTACAGAGGACTTTTTTTGCGCAACTGTGGACACTTTCAATGGTGCCTTGAAATCTATGACCTCAACTT
 241 TTCAAAAGACTTTTTTCAATGTTATTTTAGCCATGTAAATAAGTGTAGATAGAGGAATTAAACTGTATATTCTGGATAAA
 321 TAAAATTATTTCGACCATGAAAAGCGGAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' gguAGAAAUGGUC----UGUCACAAu 5'
             ||||| :::|    || ||||| 
Target 5' ctcTCTTTTTTGGGACTACTGTGTTt 3'
 		22 - 47 129.00 -10.10
2 
miRNA  3' gguAGAAAUGGUCU-------GUCACAAu 5'
             |:|| |  |||       || |||| 
Target 5' aacTTTTCAAAAGACTTTTTTCAATGTTa 3'
 		236 - 264 115.00 -5.90
3 
miRNA  3' ggUAGAAAUGGU-C---U-GUCACAau 5'
            ||:|| :||| |   |  |||||  
Target 5' ttATTTTAGCCATGTAAATAAGTGTag 3'
 		262 - 288 104.00 -8.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-141 :: DLX5    [ Functional MTI ]
Validation Method Luciferase reporter assay
Article - Itoh, T. Nozawa, Y. Akao, Y.
- J Biol Chem, 2009
MicroRNAs (miRs) are endogenously expressed 18-25-nucleotide RNAs that regulate gene expression through translational repression by binding to a target mRNA. Recently, it was indicated that miRs act as key regulators in cell differentiation, cell growth, and cell death. In osteogenesis, several miRs (for example miR-26a, -125b, -133, and -135) regulate osteoblast cell growth or differentiation in human adipose tissue-derived stem cells, mouse mesenchymal ST2 stem cells, and mouse premyogenic C2C12 cells. Additionally, Smad proteins control Drosha-mediated miR maturation. Therefore, miRs are closely related to osteogenesis. Here we investigated miR expression profile by an miR array and identified the candidate miRs, miR-141 and -200a, as pre-osteoblast differentiation-related miRs. The effects of miR-141 and -200a on pre-osteoblast differentiation were examined by using transfection of murine pre-osteoblastic MC3T3-E1 cells with mature miR-141 or -200a and antisense inhibitor for miR-141 or -200a. It was shown that miR-141 and -200a remarkably modulated the BMP-2-induced pre-osteoblast differentiation through the translational repression of Dlx5, which is a bone-generating transcription factor expressed in pre-osteoblast differentiation. Furthermore, it was indicated that Dlx5 is a common target of miR-141 and -200a by using a luciferase reporter assay. Thus, we have observed for the first time that miR-141 and -200a are involved in pre-osteoblast differentiation in part by regulating the expression of Dlx5.
LinkOut: [PMID: 19454767]