Accession ID: MIRT000390 [miRNA, hsa-miR-1 ::
CEBPA, target gene]
| pre-miRNA ID | hsa-mir-1-2 LinkOut: [miRBase ] |
|---|---|
| Synonyms | MIRN1-2, hsa-mir-1-2, miRNA1-2, MIR1-2 |
| Description | Homo sapiens miR-1-2 stem-loop |
| Comment | Lagos-Quintana et al. . |
| 2nd Structure of pre-miRNA |  |
| Mature miRNA | hsa-miR-1 |
|---|---|
| Mature Sequence | 53| UGGAAUGUAAAGAAGUAUGUAU |74 |
| Evidence | Experimental |
| Experiments | Cloned |
| Expression Profile |  |
| Putative hsa-miR-1 Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
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| Gene Symbol | CEBPA LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Synonyms | C/EBP-alpha, CEBP | ||||||||||||||||||||
| Description | CCAAT/enhancer binding protein (C/EBP), alpha | ||||||||||||||||||||
| Transcript | NM_004364 LinkOut: [ RefSeq ] | ||||||||||||||||||||
| Expression | LinkOut: [ BioGPS ] | ||||||||||||||||||||
| KEGG Pathway |
hsa05200 Pathways in cancer - Homo sapiens (human) hsa05221 Acute myeloid leukemia - Homo sapiens (human) |
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| Putative miRNA Targets on CEBPA | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] | ||||||||||||||||||||
| 3'UTR of CEBPA (miRNA target sites are highlighted) |
>CEBPA|NM_004364|3'UTR 1 TGAGGCGCGCGGCTGTGGGACCGCCCTGGGCCAGCCTCCGGCGGGGACCCAGGGAGTGGTTTGGGGTCGCCGGATCTCGA 81 GGCTTGCCCGAGCCGTGCGAGCCAGGACTAGGAGATTCCGGTGCCTCCTGAAAGCCTGGCCTGCTCCGCGTGTCCCCTCC 161 CTTCCTCTGCGCCGGACTTGGTGCGTCTAAGATGAGGGGGCCAGGCGGTGGCTTCTCCCTGCGAGGAGGGGAGAATTCTT 241 GGGGCTGAGCTGGGAGCCCGGCAACTCTAGTATTTAGGATAACCTTGTGCCTTGGAAATGCAAACTCACCGCTCCAATGC 321 CTACTGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTGCCTCCTTCCCGAGGCTACAGCAGACC 401 CCCATGAGAGAAGGAGGGGAGCAGGCCCGTGGCAGGAGGAGGGCTCAGGGAGCTGAGATCCCGACAAGCCCGCCAGCCCC 481 AGCCGCTCCTCCACGCCTGTCCTTAGAAAGGGGTGGAAACATAGGGACTTGGGGCTTGGAACCTAAGGTTGTTCCCCTAG 561 TTCTACATGAAGGTGGAGGGTCTCTAGTTCCACGCCTCTCCCACCTCCCTCCGCACACACCCCACCCCAGCCTGCTATAG 641 GCTGGGCTTCCCCTTGGGGCGGAACTCACTGCGATGGGGGTCACCAGGTGACCAGTGGGAGCCCCCACCCCGAGTCACAC 721 CAGAAAGCTAGGTCGTGGGTCAGCTCTGAGGATGTATACCCCTGGTGGGAGAGGGAGACCTAGAGATCTGGCTGTGGGGC 801 GGGCATGGGGGGTGAAGGGCCACTGGGACCCTCAGCCTTGTTTGTACTGTATGCCTTCAGCATTGCCTAGGAACACGAAG 881 CACGATCAGTCCATCCCAGAGGGACCGGAGTTATGACAAGCTTTCCAAATATTTTGCTTTATCAGCCGATATCAACACTT 961 GTATCTGGCCTCTGTGCCCCAGCAGTGCCTTGTGCAATGTGAATGTGCGCGTCTCTGCTAAACCACCATTTTATTTGGTT 1041 TTTGTTTTGTTTTGGTTTTGCTCGGATACTTGCCAAAATGAGACTCTCCGTCGGCAGCTGGGGGAAGGGTCTGAGACTCC 1121 CTTTCCTTTTGGTTTTGGGATTACTTTTGATCCTGGGGGACCAATGAGGTGAGGGGGGTTCTCCTTTGCCCTCAGCTTTC 1201 CCCAGCCCCTCCGGCCTGGGCTGCCCACAAGGCTTGTCCCCCAGAGGCCCTGGCTCCTGGTCGGGAAGGGAGGTGGCCTC 1281 CCGCCAACGCATCACTGGGGCTGGGAGCAGGGAAGGACGGCTTGGTTCTCTTCTTTTGGGGAGAACGTAGAGTCTCACTC 1361 TAGATGTTTTATGTATTATATCTATAATATAAACATATCAAAGTCAA Target sites Provided by authors Predicted by miRanda |
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| miRNA-target interactions (Predicted by miRanda) |
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| miRNA:Target | hsa-miR-1 :: CEBPA [ Functional MTI ] |
|---|---|
| Validation Method | Luciferase reporter assay |
| Article |
- Nasser, M. W.
Datta, J.
Nuovo, G.
Kutay, H. et al. - J Biol Chem, 2008
Micro-RNAs are approximately 21-25-nucleotide-long noncoding RNAs that regulate gene expression primarily at the post-transcriptional level in animals. Here, we report that micro-RNA-1 (miR-1), abundant in the cardiac and smooth muscles, is expressed in the lung and is down-regulated in human primary lung cancer tissues and cell lines. In situ hybridization demonstrated localization of miR-1 in bronchial epithelial cells. The tumor suppressor C/EBPalpha, frequently suppressed in lung cancer, reactivated miR-1 expression in the lung cancer cells. Repressed miR-1 was also activated in lung cancer cells upon treatment with a histone deacetylase inhibitor. These observations led us to examine the antitumorigenic potential of miR-1 in lung cancer cells. Expression of miR-1 in nonexpressing A549 and H1299 cells reversed their tumorigenic properties, such as growth, replication potential, motility/migration, clonogenic survival, and tumor formation in nude mice. Exogenous miR-1 significantly reduced expression of oncogenic targets, such as MET, a receptor tyrosine kinase, and Pim-1, a Ser/Thr kinase, frequently up-regulated in lung cancer. Similarly, the levels of two additional targets, FoxP1, a transcription factor with oncogeneic property, and HDAC4 that represses differentiation-promoting genes, were reduced in miR-1-expressing cells. Conversely, depletion of miR-1 facilitated N417 cell growth with concomitant elevation of these targets. Further, ectopic miR-1 induced apoptosis in A549 cells in response to the potent anticancer drug doxorubicin. Enhanced activation of caspases 3 and 7, cleavage of their substrate PARP-1, and depletion of anti-apoptotic Mcl-1 contributed to the sensitivity of miR-1-expressing cells to doxorubicin. Thus, miR-1 has potential therapeutic application against lung cancers.
LinkOut: [PMID: 18818206]
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