Accession ID: MIRT000441 [miRNA, hsa-miR-802 :: MECP2, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-802LinkOut: [miRBase ]
Synonyms MIRN802, hsa-mir-802, MIR802
Description Homo sapiens miR-802 stem-loop
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-802
Evidence Experimental
Experiments MiRAP-cloned
Putative hsa-miR-802 Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Transcript    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of
(miRNA target sites are highlighted)
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-802 :: MECP2    [ Functional MTI ]
Validation Method Immunohistochemistry , Luciferase reporter assay , qRT-PCR , Western blot ,
Conditions SK-N-SH
Location of target site 3'UTR
Tools used in this research miRBase Target Database , PicTar , PITA , TargetScan
Original Description (Extracted from the article) ... miR-802 and the mutant construct co-transfection experiments demonstrated that miR-802 can interfere with luciferase expression via direct interaction with only the second miR-802 site (i.e. 6875– 6896 bp) in this in vitro surrogate assay// ...

- Kuhn, D. E. Nuovo, G. J. Terry, A. V., Jr. et al., 2010, J Biol Chem.

Article - Kuhn, D. E. Nuovo, G. J. Terry, A. V., Jr. et al.
- J Biol Chem, 2010
Down syndrome (DS), or Trisomy 21, is the most common genetic cause of cognitive impairment and congenital heart defects in the human population. Bioinformatic annotation has established that human chromosome 21 (Hsa21) harbors five microRNA (miRNAs) genes: miR-99a, let-7c, miR-125b-2, miR-155, and miR-802. Our laboratory recently demonstrated that Hsa21-derived miRNAs are overexpressed in DS brain and heart specimens. The aim of this study was to identify important Hsa21-derived miRNA/mRNA target pairs that may play a role, in part, in mediating the DS phenotype. We demonstrate by luciferase/target mRNA 3'-untranslated region reporter assays, and gain- and loss-of-function experiments that miR-155 and -802 can regulate the expression of the predicted mRNA target, the methyl-CpG-binding protein (MeCP2). We also demonstrate that MeCP2 is underexpressed in DS brain specimens isolated from either humans or mice. We further demonstrate that, as a consequence of attenuated MeCP2 expression, transcriptionally activated and silenced MeCP2 target genes, CREB1/Creb1 and MEF2C/Mef2c, are also aberrantly expressed in these DS brain specimens. Finally, in vivo silencing of endogenous miR-155 or -802, by antagomir intra-ventricular injection, resulted in the normalization of MeCP2 and MeCP2 target gene expression. Taken together, these results suggest that improper repression of MeCP2, secondary to trisomic overexpression of Hsa21-derived miRNAs, may contribute, in part, to the abnormalities in the neurochemistry observed in the brains of DS individuals. Finally these results suggest that selective inactivation of Hsa21-derived miRNAs may provide a novel therapeutic tool in the treatment of DS.
LinkOut: [PMID: 19897480]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-802 :: MECP2    [ Functional MTI ]
Validation Method Luciferase reporter assay , qRT-PCR , Microarray
Location of target site 3'UTR
Original Description (Extracted from the article) ... Recently, Kuhn et al. (2008) used microarrays, RT-PCR and miRNA in situ hybridization methods to investigate five miRNA genes (miR-99a, let-7c, miR-125b-2, miR-155, and miR-802) located on human chromosome 21. They showed that these miRNAs were up-regulated in the fetal brain tissue of DS patients compared to age- and sex-matched controls. In a follow-up study, the same group used luciferase/target mRNA 3′UTR reporter assays to demonstrate that miR-155 and miR-802 can regulate MeCP2 expression. ...

- Xu, B. Karayiorgou, M. Gogos, J. A., 2010, Brain Res.

Article - Xu, B. Karayiorgou, M. Gogos, J. A.
- Brain Res, 2010
Abnormalities in microRNA (miRNA)-mediated gene regulation have been observed in a variety of human diseases, especially in cancer. Here, we provide an account of newly emerging connections between miRNAs with various psychiatric and neurodevelopmental disorders, including recent findings of miRNA dysregulation in the 22q11.2 microdeletion syndrome, a well-established genetic risk factor for schizophrenia. miRNAs appear to be components of both the genetic architecture of these complex phenotypes as well as integral parts of the biological pathways that mediate the effects of primary genetic deficits. Therefore, they may contribute to both genetic heterogeneity and phenotypic variation of psychiatric and neurodevelopmental disorders and could serve as novel therapeutic targets.
LinkOut: [PMID: 20388499]
MiRNA-Target Expression Profile:

MiRNA-Target Expression Profile(TCGA):

MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
57 hsa-miR-802 Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000441 MECP2 methyl CpG binding protein 2 (Rett syndrome) 5 2
MIRT058640 CAPZA1 capping protein (actin filament) muscle Z-line, alpha 1 1 1
MIRT071879 BTF3L4 basic transcription factor 3-like 4 1 1
MIRT085671 EPC2 enhancer of polycomb homolog 2 (Drosophila) 1 1
MIRT100707 TJAP1 tight junction associated protein 1 (peripheral) 1 1
MIRT111738 ALDH9A1 aldehyde dehydrogenase 9 family, member A1 1 1
MIRT147311 KPNA2 karyopherin alpha 2 (RAG cohort 1, importin alpha 1) 1 5
MIRT183599 SNRPE small nuclear ribonucleoprotein polypeptide E 1 2
MIRT226406 TP53INP1 tumor protein p53 inducible nuclear protein 1 1 1
MIRT230754 PAFAH1B2 platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa) 1 3
MIRT295105 PTBP1 polypyrimidine tract binding protein 1 1 1
MIRT366444 KLHL15 kelch-like 15 (Drosophila) 1 3
MIRT367101 ZNF711 zinc finger protein 711 1 1
MIRT443239 ANKRD26 ankyrin repeat domain 26 1 1
MIRT450887 COL4A5 collagen, type IV, alpha 5 1 1
MIRT454707 HMGN1 high mobility group nucleosome binding domain 1 1 2
MIRT456147 RWDD2A RWD domain containing 2A 1 7
MIRT457419 CASC5 cancer susceptibility candidate 5 1 1
MIRT466171 TMED5 transmembrane emp24 protein transport domain containing 5 1 2
MIRT467222 SPRED1 sprouty-related, EVH1 domain containing 1 1 1
MIRT469011 RNF41 ring finger protein 41 1 1
MIRT470410 PPP1R15B protein phosphatase 1, regulatory subunit 15B 1 1
MIRT481590 ARID2 AT rich interactive domain 2 (ARID, RFX-like) 1 1
MIRT488602 FAM3C family with sequence similarity 3, member C 1 4
MIRT497952 DENND1B DENN/MADD domain containing 1B 1 1
MIRT499513 RNF6 ring finger protein (C3H2C3 type) 6 1 3
MIRT500156 CREBBP CREB binding protein 1 1
MIRT503529 ATG12 autophagy related 12 1 2
MIRT506179 PHTF2 putative homeodomain transcription factor 2 1 2
MIRT509274 NPM3 nucleophosmin/nucleoplasmin 3 1 3
MIRT514990 DNTTIP2 deoxynucleotidyltransferase, terminal, interacting protein 2 1 1
MIRT519097 SLC4A1 solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group) 1 1
MIRT524837 ARPP19 cAMP-regulated phosphoprotein, 19kDa 1 1
MIRT527927 FRY furry homolog (Drosophila) 1 1
MIRT531360 FAM81B family with sequence similarity 81, member B 1 1
MIRT536828 HMBOX1 homeobox containing 1 1 1
MIRT537395 FGD6 FYVE, RhoGEF and PH domain containing 6 1 1
MIRT539349 AGO2 eukaryotic translation initiation factor 2C, 2 1 2
MIRT547672 KPNA1 karyopherin alpha 1 (importin alpha 5) 1 2
MIRT549935 RPL7L1 ribosomal protein L7-like 1 1 2
MIRT551841 AASDHPPT aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase 1 1
MIRT553827 SYNCRIP synaptotagmin binding, cytoplasmic RNA interacting protein 1 1
MIRT554210 SLC35A5 solute carrier family 35, member A5 1 1
MIRT560269 SF1 splicing factor 1 1 1
MIRT566762 MLLT10 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 10 1 1
MIRT567401 GRPEL2 GrpE-like 2, mitochondrial (E. coli) 1 1
MIRT571710 RPL17-C18orf32 RPL17-C18orf32 readthrough 1 1
MIRT572196 C18orf32 chromosome 18 open reading frame 32 1 1
MIRT573378 KIAA1549L KIAA1549-like 1 1
MIRT573615 ZNF724P zinc finger protein 724, pseudogene 1 1
MIRT573977 DDX21 DEAD (Asp-Glu-Ala-Asp) box helicase 21 1 1
MIRT609217 PELP1 proline, glutamate and leucine rich protein 1 1 3
MIRT612357 TNPO1 transportin 1 1 1
MIRT698459 TJP1 tight junction protein 1 1 1
MIRT699948 RTN3 reticulon 3 1 1
MIRT704221 DIS3 DIS3 mitotic control homolog (S. cerevisiae) 1 1
MIRT712025 STX1B syntaxin 1B 1 1
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