Accession ID: MIRT000497 [miRNA, hsa-miR-20b-5p :: STAT3, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-20bLinkOut: [miRBase ]
Synonyms MIRN20B, hsa-mir-20b, MIR20B
Description Homo sapiens miR-20b stem-loop
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-20b-5p
Mature Sequence 6| CAAAGUGCUCAUAGUGCAGGUAG |28
Evidence Experimental
Experiments Array-cloned
Putative hsa-miR-20b-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol STAT3 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms APRF, FLJ20882, HIES, MGC16063
Description signal transducer and activator of transcription 3 (acute-phase response factor)
Transcript NM_2136    LinkOut: [ RefSeq ]
Other Transcripts NM_1392 , NM_0031   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on STAT3 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of STAT3
(miRNA target sites are highlighted)
>STAT3|NM_2136|3'UTR
   1 TAATGGTGAAGGTGCTGAACCCTCAGCAGGAGGGCAGTTTGAGTCCCTCACCTTTGACATGGAGTTGACCTCGGAGTGCG
  81 CTACCTCCCCCATGTGAGGAGCTGAGAACGGAAGCTGCAGAAAGATACGACTGAGGCGCCTACCTGCATTCTGCCACCCC
 161 TCACACAGCCAAACCCCAGATCATCTGAAACTACTAACTTTGTGGTTCCAGATTTTTTTTAATCTCCTACTTCTGCTATC
 241 TTTGAGCAATCTGGGCACTTTTAAAAATAGAGAAATGAGTGAATGTGGGTGATCTGCTTTTATCTAAATGCAAATAAGGA
 321 TGTGTTCTCTGAGACCCATGATCAGGGGATGTGGCGGGGGGTGGCTAGAGGGAGAAAAAGGAAATGTCTTGTGTTGTTTT
 401 GTTCCCCTGCCCTCCTTTCTCAGCAGCTTTTTGTTATTGTTGTTGTTGTTCTTAGACAAGTGCCTCCTGGTGCCTGCGGC
 481 ATCCTTCTGCCTGTTTCTGTAAGCAAATGCCACAGGCCACCTATAGCTACATACTCCTGGCATTGCACTTTTTAACCTTG
 561 CTGACATCCAAATAGAAGATAGGACTATCTAAGCCCTAGGTTTCTTTTTAAATTAAGAAATAATAACAATTAAAGGGCAA
 641 AAAACACTGTATCAGCATAGCCTTTCTGTATTTAAGAAACTTAAGCAGCCGGGCATGGTGGCTCACGCCTGTAATCCCAG
 721 CACTTTGGGAGGCCGAGGCGGATCATAAGGTCAGGAGATCAAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACT
 801 AAAAGTACAAAAAATTAGCTGGGTGTGGTGGTGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC
 881 TTGAACCTGAGAGGCGGAGGTTGCAGTGAGCCAAAATTGCACCACTGCACACTGCACTCCATCCTGGGCGACAGTCTGAG
 961 ACTCTGTCTCAAAAAAAAAAAAAAAAAAAAGAAACTTCAGTTAACAGCCTCCTTGGTGCTTTAAGCATTCAGCTTCCTTC
1041 AGGCTGGTAATTTATATAATCCCTGAAACGGGCTTCAGGTCAAACCCTTAAGACATCTGAAGCTGCAACCTGGCCTTTGG
1121 TGTTGAAATAGGAAGGTTTAAGGAGAATCTAAGCATTTTAGACTTTTTTTTATAAATAGACTTATTTTCCTTTGTAATGT
1201 ATTGGCCTTTTAGTGAGTAAGGCTGGGCAGAGGGTGCTTACAACCTTGACTCCCTTTCTCCCTGGACTTGATCTGCTGTT
1281 TCAGAGGCTAGGTTGTTTCTGTGGGTGCCTTATCAGGGCTGGGATACTTCTGATTCTGGCTTCCTTCCTGCCCCACCCTC
1361 CCGACCCCAGTCCCCCTGATCCTGCTAGAGGCATGTCTCCTTGCGTGTCTAAAGGTCCCTCATCCTGTTTGTTTTAGGAA
1441 TCCTGGTCTCAGGACCTCATGGAAGAAGAGGGGGAGAGAGTTACAGGTTGGACATGATGCACACTATGGGGCCCCAGCGA
1521 CGTGTCTGGTTGAGCTCAGGGAATATGGTTCTTAGCCAGTTTCTTGGTGATATCCAGTGGCACTTGTAATGGCGTCTTCA
1601 TTCAGTTCATGCAGGGCAAAGGCTTACTGATAAACTTGAGTCTGCCCTCGTATGAGGGTGTATACCTGGCCTCCCTCTGA
1681 GGCTGGTGACTCCTCCCTGCTGGGGCCCCACAGGTGAGGCAGAACAGCTAGAGGGCCTCCCCGCCTGCCCGCCTTGGCTG
1761 GCTAGCTCGCCTCTCCTGTGCGTATGGGAACACCTAGCACGTGCTGGATGGGCTGCCTCTGACTCAGAGGCATGGCCGGA
1841 TTTGGCAACTCAAAACCACCTTGCCTCAGCTGATCAGAGTTTCTGTGGAATTCTGTTTGTTAAATCAAATTAGCTGGTCT
1921 CTGAATTAAGGGGGAGACGACCTTCTCTAAGATGAACAGGGTTCGCCCCAGTCCTCCTGCCTGGAGACAGTTGATGTGTC
2001 ATGCAGAGCTCTTACTTCTCCAGCAACACTCTTCAGTACATAATAAGCTTAACTGATAAACAGAATATTTAGAAAGGTGA
2081 GACTTGGGCTTACCATTGGGTTTAAATCATAGGGACCTAGGGCGAGGGTTCAGGGCTTCTCTGGAGCAGATATTGTCAAG
2161 TTCATGGCCTTAGGTAGCATGTATCTGGTCTTAACTCTGATTGTAGCAAAAGTTCTGAGAGGAGCTGAGCCCTGTTGTGG
2241 CCCATTAAAGAACAGGGTCCTCAGGCCCTGCCCGCTTCCTGTCCACTGCCCCCTCCCCATCCCCAGCCCAGCCGAGGGAA
2321 TCCCGTGGGTTGCTTACCTACCTATAAGGTGGTTTATAAGCTGCTGTCCTGGCCACTGCATTCAAATTCCAATGTGTACT
2401 TCATAGTGTAAAAATTTATATTATTGTGAGGTTTTTTGTCTTTTTTTTTTTTTTTTTTTTTTGGTATATTGCTGTATCTA
2481 CTTTAACTTCCAGAAATAAACGTTATATAGGAACCGTAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' gaUGGA----CGUGAUACUCGUGAAAc 5'
            |:||    ||| | ||:||||||| 
Target 5' ctATCTTTGAGCAATCTGGGCACTTTt 3'
236 - 262 167.00 -17.70
2
miRNA  3' gaUGGACGUGAUACUCGUGAAAc 5'
            :||||:| |   |||||||| 
Target 5' acGCCTGTAATCCCAGCACTTTg 3'
705 - 727 165.00 -20.90
3
miRNA  3' gauGGA-CGUGAUACUCGUGAAAc 5'
             ||| |||:|    ||||||| 
Target 5' actCCTGGCATT----GCACTTTt 3'
533 - 552 154.00 -14.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-20b-5p :: STAT3    [ Functional MTI ]
Validation Method qRT-PCR , ELISA , ChIP , Western blot , Other
Conditions MCF-7
Location of target site 3'UTR
Original Description (Extracted from the article) ... In summary, this report demonstrates, for the first time, that the VEGF expression in breast cancer cells is mediated by HIF-1 and STAT3 in a miR-20b-dependent manner.//Fig. 1. MiR-20b is downregulated during hypoxia and regulates VEGF mRNA expression.//In the present study, we identified VEGF and its two main hypoxia-inducible transcriptional activators, HIF-1a and STAT3, as targets for miR-20b in MCF-7 breast cancer cells. ...

- Cascio, S. D'Andrea, A. Ferla, R. Surmacz, et al., 2010, J Cell Physiol.

Article - Cascio, S. D'Andrea, A. Ferla, R. Surmacz, et al.
- J Cell Physiol, 2010
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the expression of different genes, including genes involved in cancer progression. A functional link between hypoxia, a key feature of the tumor microenvironment, and miRNA expression has been documented. We investigated whether and how miR-20b can regulate the expression of vascular endothelial growth factor (VEGF) in MCF-7 breast cancer cells under normoxic and hypoxia-mimicking conditions (CoCl(2) exposure). Using immunoblotting, ELISA, and quantitative real-time PCR, we demonstrated that miR-20b decreased VEGF protein levels at 4 and 24 h following CoCl(2) treatment, and VEGF mRNA at 4 h of treatment. In addition, miR-20b reduced VEGF protein expression in untreated cells. Next, we investigated the molecular mechanism by which pre-miR-20b can affect VEGF transcription, focusing on hypoxia inducible factor 1 (HIF-1) and signal transducer and activator of transcription 3 (STAT3), transcriptional inducers of VEGF and putative targets of miR-20b. Downregulation of VEGF mRNA by miR-20b under a 4 h of CoCl(2) treatment was associated with reduced levels of nuclear HIF-1 alpha subunit and STAT3. Chromatin immunoprecipitation (ChIP) assays revealed that HIF-1 alpha, but not STAT3, was recruited to the VEGF promoter following the 4 h of CoCl(2) treatment. This effect was inhibited by transfection of cells with pre-miR-20b. In addition, using siRNA knockdown, we demonstrated that the presence of STAT3 is necessary for CoCl(2)-mediated HIF-1 alpha nuclear accumulation and recruitment on VEGF promoter. In summary, this report demonstrates, for the first time, that the VEGF expression in breast cancer cells is mediated by HIF-1 and STAT3 in a miR-20b-dependent manner.
LinkOut: [PMID: 20232316]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
17 hsa-miR-20b-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000497 STAT3 signal transducer and activator of transcription 3 (acute-phase response factor) 3 1
MIRT000498 HIF1A hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) 3 1
MIRT000596 CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) 4 2
MIRT001782 HIPK3 homeodomain interacting protein kinase 3 2 1
MIRT001783 MYLIP myosin regulatory light chain interacting protein 3 1
MIRT004000 PPARG peroxisome proliferator-activated receptor gamma 3 1
MIRT004053 CRIM1 cysteine rich transmembrane BMP regulator 1 (chordin-like) 3 1
MIRT004072 ARID4B AT rich interactive domain 4B (RBP1-like) 3 1
MIRT004121 BAMBI BMP and activin membrane-bound inhibitor homolog (Xenopus laevis) 3 1
MIRT004313 ESR1 estrogen receptor 1 4 1
MIRT004451 VEGFA vascular endothelial growth factor A 2 1
MIRT004712 MUC17 mucin 17, cell surface associated 3 1
MIRT006337 EFNB2 ephrin-B2 1 1
MIRT006338 EPHB4 EPH receptor B4 1 1
MIRT042399 HDGF hepatoma-derived growth factor 1 1
MIRT042400 NFATC2IP nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein 1 1
MIRT042401 PSD3 pleckstrin and Sec7 domain containing 3 1 1