Accession ID: MIRT000639 [miRNA, hsa-miR-9 ::
POU2F2, target gene]
| miRNA name | hsa-miR-9 |
|---|
 |
| Gene Symbol | POU2F2 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] |
|---|---|
| Synonyms | OCT2, OTF2, Oct-2 |
| Description | POU class 2 homeobox 2 |
| Transcript | NM_002698 LinkOut: [ RefSeq ] |
| Expression | LinkOut: [ BioGPS ] |
| Putative miRNA Targets on POU2F2 | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] |
| 3'UTR of POU2F2 (miRNA target sites are highlighted) |
>POU2F2|NM_002698|3'UTR 1 TGATGGCAGCGGGAATCTGGTGCTGGGGGCAGCCGGTGCAGCCCCGGGGAGCCCTGGCCTGGTGACCTCGCCGCTCTTCT 81 TGAATCATGCTGGGCTGCCCCTGCTCAGCACCCCGCCTGGTGTGGGCCTGGTCTCAGCAGCGGCTGCGGCTGTGGCAGCC 161 TCCATCTCCAGCAAGTCTCCTGGCCTCTCCTCCTCATCCTCTTCATCCTCATCCTCCTCCTCCTCCACTTGCAGCGAGAC 241 GGCAGCACAGACCCCTGGAGGTCCAGGGGGGCCCGAGGCAGGGTCCAAACCTGAGTGAGGGCCAGCCATGCCTCCCCTCC 321 CATTCCTCTGGTCCCTGCCTTGGTCCCTTGCCTGGGAAGAGGGCGAGGAGGCCAGTGGTGGGGACGCAGAGGGTCCTCAG 401 AGCAGGAGTGACAAGGGAGGAAAGACCAAAAAAACAACCAACCAAAAAAAAAAAAAAAAAGGAAAGAAACTAACCAACAA 481 AAGAGAAAACCAAAAATAATCACAACAGAAACCAGCTGCCCCAAAGGAACCAGAGGTGAAAAACAAACAAAAAAAAACCA 561 AAAACAAACCAAAAAAAAAAAAAACCCACAAAATCAAACAAACCAAAAAACCAGTCGCGAGCCAGACCTCAGCGTGCTCA 641 CCCTCACTGCTACGACGCCAAATAAAAACCCCAGCCAGGGGCGGAGAAGCCTCCAGCAGGTCAGACCTCATGCCACCGAG 721 CCCTGGCTGTGGGACCAACCCCCAACCCTGCCTCCCCCGTGGGGGCTACAGAAGGAAAAAGAGAAGATGCCAGCTTCCTA 801 ATCCCAGCCCCCAGCCCTGGGCCAGCGAAGACAGGGCACAGCCTGGGCAGATGGGCTGGGGCTTAGCACCACCCACCAAA 881 TGTTCTTTTCCAGAAGGTGAAAGAGAAAGGGCCTGAACAACCTTACACCAAATATTCAGTAGCTTCATCCAAAGGATGTA 961 CAGAATTTTTAGCATTGTGCTCAACAGAATGTGTCCCTACCATGTGTCCCCTTCTCCCCTGGCCCCCAGCTCTCCCCACC 1041 TGGGCAGGGGGTCTTGCTTTAACCTCCTCCCTCCCCCCAGCAGGGGAGAGTTCAAGGGAAGGCCTGCGGACAACTTTTCA 1121 TCCCCTGTTCCTCCCTCTTTTCCCCTTTGAAGGGTGGGCTAGGCCATTTTGCCAAGTTCTAGCTCTCACAAGTCCCTCCT 1201 CAACCCTGTCACCCTCCTCTGCTCTGGAACTGACTCCCTCCCCAGCCTATGGGAAGGTGGAAATTTCAGGCAAGAGGGGA 1281 TGAAGACATTCAGTTGAGGGCATTCAGTTGTCTTTTTCCATCCTGTCTGTTTCCCTGAAAAAAAAAAAAATTCATATTCC 1361 AGTGCCTATCCGTGGGATCCTTCACGTTCTTTGACATTAACCAGAAACCAAAAAGAGAACTCACCTCGCTTTTCCCACCC 1441 TGTGCCCCTCTGGTACTGGCGAATGCCTCTCCCTCCCCCCACATGCACGCACGCACACACCCCAGTGGTGGGGTTCCTCG 1521 AGATGGCATCCCCATCAGGGTCCATGTGGTGGGGACAGTGCCACAGCCTGTGGTCCCCATCTGAGAGGGCGCGGTGGTGG 1601 CCACCACTCCCACAAGACCTCCACAACTCTTGGCTGGACCCTGTGTTTGACCAGCCCCGGACACGTCTCCACTGACGACG 1681 GACAGAGGAGAGACACCACTGGGAGCCACCCTGCCCTCCTGCTATTGTGGAGGCCAACAAAGTTTTGAACCGAAAACCAA 1761 AAAAACAGAAACAAACAAACAATAAATTTAAACTAGAAAAAAAATTTATATATATATATAAACATATATATATATATATA 1841 TATAAAAGGGAAAGAAGATGAGGACTCTTCAAGAATAAGATGGAACCGCGAGGCGGAGCCAAGCCCCTGCACCGGTGGTC 1921 CAGGCCCTTGTTCCCCAAGGCGGATGGAAGGACGGATGCTTCTTTCTCTTCACAAATACCTCATGGACGTTGTCTTTGGG 2001 AAAGCCGGAGGGGGCGGCTGGGGCAGGGCCTGTCCCTCGGCCAGGGCAGATGGAAGCGGGTGGGCGGGGCTGAGAGAGGG 2081 TATCAGGGACAGGGGTGAAGAGCCCCAAACTCCCCTCCCAAATCAACTGAAAAAGCTTTAAAAAAAGACAGGAAAAAAAG 2161 GAGTAAGAAAGCAAAAAGAACAGATGAAGGAAAACTTAACAACTTTTGGGTGGTTTGATTCCTCCTTTGATTTCTTTTTC 2241 GGTTCTCTTTTGTCTGACCTCTCTCCTCCCCTCCTTCCTCTCTCCTCCCCTCCTTCCTCTCTCCTCTCCTCCTTCCTGTC 2321 TCCTCTCTCTCCCTTTGCTCTCCTCTCTTCTCTCTCAATCTTGTTCTTTCTGTGTCTCTCCTCAAACCAAAGTGTTGCAG 2401 TGAAGGACACGAGCCATTGAAATCAGGGTGGGGCCTGACAGCCGTAGTGTGGCCCCTGCCCCTTGTTGGGCAGGAGCAGA 2481 GAGAGAGGAGAGCCCTGAGACCGCAGGGCTTTGGCCTGGGCAGCTTCCACTTTCTGCCGGACACTCTGAGGAGAGGCAGA 2561 TGGAGCTCCAGGCCTCAGCGTTCTCTTTTTTCCTTGTCTCCCTTCTCCCACCCGGGAAATGAAACTGTTGGGCTGGGCGA 2641 CGGAGGCAGCAGAGACTCGGCCATTGCAGGGGCCTCTGGGTGCCTTGTCCGGGCAGTGGTGAAGTAGCCGCCCCTCCCAC 2721 CCCGGCACGATGGGGCCTGGCACCTCGTCTACCCAACCTCACCGGAATGTAAGCATCTCCGCTGAACGACTCCCTGCCCT 2801 TACCCTACCTCTGAGTTTGTCCATGTTTATTTCCTGAAGAGAGAAGGGACTGGCAAGAGGGCTTGGGCCCCAGCCCAAGG 2881 GTGGAGAGGGGGCCAAGGGCTCCTGACCAAATAGGAAGGACATTTGAGCAGAGCAAAATGAAAGGAATTACAACCAAAAA 2961 CCCCCTCCGAGAAGACAGGCAGCATGGAAGGCATGGTGGAGATGACTCAAACAAAAACTATGATTCTAGACCAAAAAGGA 3041 AAAAAAAAAAAAAAAAA Target sites Provided by authors Predicted by miRanda |
| miRNA:Target | hsa-miR-9 :: POU2F2 [ Non-Functional MTI ] |
|---|---|
| Validation Method | Luciferase reporter assay |
| Conditions | HeLa |
| Location of target site | 3'UTR |
| Tools used in this research | EIMMO, TargetScan |
| Original Description (Extracted from the article) | ... we find the following: mir-424 targets ANLN, CCNE1, CCND1,WEE1, ATF6, KIF23, CHEK1, CDC25A, CDC14A and CCNF whereas mir-503 targets CDC14A, ANLN, CCND1, ATF6,EIF2C1, CDC25A, CHEK1, CCNE1, CCNE2, WEE1, CCNF and CDKN1A. ... - Forrest, A. R. Kanamori-Katayama, M. et al., 2010, Leukemia. |
| Article |
- Forrest, A. R.
Kanamori-Katayama, M. et al. - Leukemia, 2010
Acute myeloid leukemia (AML) involves a block in terminal differentiation of the myeloid lineage and uncontrolled proliferation of a progenitor state. Using phorbol myristate acetate (PMA), it is possible to overcome this block in THP-1 cells (an M5-AML containing the MLL-MLLT3 fusion), resulting in differentiation to an adherent monocytic phenotype. As part of FANTOM4, we used microarrays to identify 23 microRNAs that are regulated by PMA. We identify four PMA-induced microRNAs (mir-155, mir-222, mir-424 and mir-503) that when overexpressed cause cell-cycle arrest and partial differentiation and when used in combination induce additional changes not seen by any individual microRNA. We further characterize these pro-differentiative microRNAs and show that mir-155 and mir-222 induce G2 arrest and apoptosis, respectively. We find mir-424 and mir-503 are derived from a polycistronic precursor mir-424-503 that is under repression by the MLL-MLLT3 leukemogenic fusion. Both of these microRNAs directly target cell-cycle regulators and induce G1 cell-cycle arrest when overexpressed in THP-1. We also find that the pro-differentiative mir-424 and mir-503 downregulate the anti-differentiative mir-9 by targeting a site in its primary transcript. Our study highlights the combinatorial effects of multiple microRNAs within cellular systems.
LinkOut: [PMID: 19956200]
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