Accession ID: MIRT001168 [miRNA, hsa-miR-124 ::
NR3C2, target gene]
| miRNA name | hsa-miR-124 |
|---|
 |
| Gene Symbol | NR3C2 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] |
|---|---|
| Synonyms | FLJ41052, MCR, MGC133092, MLR, MR, NR3C2VIT |
| Description | nuclear receptor subfamily 3, group C, member 2 |
| Transcript | NM_000901 LinkOut: [ RefSeq ] |
| Other Transcripts | NM_001166104 |
| Expression | LinkOut: [ BioGPS ] |
| KEGG Pathway |
hsa04960 Aldosterone-regulated sodium reabsorption - Homo sapiens (human) |
| Putative miRNA Targets on NR3C2 | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] |
| 3'UTR of NR3C2 (miRNA target sites are highlighted) |
>NR3C2|NM_000901|3'UTR 1 TGACTGCCCGCTGCCCAGAAGAACTTTGCCTTAAGTTTCCCTGTGTTGTTCCACACCCAGAAGGACCCAAGAAAACCTGT 81 TTTTAACATGTGATGGTTGATTCACACTTGTTCAACAGTTTCTCAAGTTTAAAGTCATGTCAGAGGTTTGGAGCCGGGAA 161 AGCTGTTTTTCCGTGGATTTGGCGAGACCAGAGCAGTCTGAAGGATTCCCCACCTCCAATCCCCCAGCGCTTAGAAACAT 241 GTTCCTGTTCCTCGGGATGAAAAGCCATATCTAGTCAATAACTCTGATTTTGATATTTTCACAGATGGAAGAAGTTTTAA 321 CTATGCCGTGTAGTTTCTGGTATCGTTCGCTTGTTTTAAAAGGGTTCAAGGACTAACGAACGTTTTAAAGCTTACCCTTG 401 GTTTGCACATAAAACGTATAGTCAATATGGGGCATTAATATTCTTTTGTTATTAAAAAAACACAAAAAAATAATAAAAAA 481 ATATATACAGATTCCTGTTGTGTAATAACAGAACTCGTGGCGTGGGGCAGCAGCTGCCTCTGAGCCCTCGCTCGTCCACG 561 GTCTTCTGCATCACTGGTATACACACTCGTTAGCGTCCATTTCTTATTTAATTAGAATGGATAAGATGATGTTAAATGCC 641 TTGGTTTGATTTCTAGTATCTATTGTGTTGGCTTTACAAATAATTTTTTGCAGTCTTTTGCTGTGCTGTACATTACTGTA 721 TGTATAAATTATGAAGGACCTGAAATAAGGTATAAGGATCTTTTGTAAATGAGACACATACAAAAAAAATCTTTAATGGT 801 TAATAGGATGAATGGGAAAGTATTTTTGAAAGAATTCTATTTTGCTGGAGACTATTTAAGTACTATCTTTGTCTAAACAA 881 GGTAATTTTTTTTTGTAAAGTGCAATGTCCTGCATGCATAATGAACCGTTTACAGTGTATTTAAGAAAGGGAAAGCTGTG 961 CCTTTTTTAGCTTCATATCTAATTTACCATTATTTTACAGTCTCTGTTGTAAATAACCACACTGAAACCTCTTCGGTTGT 1041 CTTGAAACCTTTCTACTTTTTCTGTACTTTTTGTTTTGTTCTTGGTCTCCCGCTTGGGGCATTTGTGGGACTCCAGCACG 1121 TTTTCTGGCTTCTGCTTCATCCTGCTCCATCGGGGAATGACACACTGCGGTGTCTGCAGCTCCTGGAAGGTGTCATTTGA 1201 CAACACATGTGGGAGAGGAGGTCCTTGGAGTGCTGCAGCTTTGGGAAAGCTGCCTCGTTTCCCTTTTCCTCTAGAAGCAG 1281 AACCAGCTCTACGAGAGTGAGACTGGGAACTTGATGGCTCAGAGAGCATCTTTTCCTCCCATTTTAGAAAATCAGATTTT 1361 CTCCTGTGGGAAAAAAAAATTCCATGCACTCTCTCTCTGTTAAAGATCAGCTATTCCCTTCTGATCTTGGAAAGAGGTTC 1441 TGCACTCCTGGAACCGGTCACAGGAACGCACAGATCATGGCAGGATGCGCTGGGACGGCCCATCTTGGCAAGGTTCAGTC 1521 TGAATGGCATGGAGACCGGGAGATAGAGGGGTTTTAGATTTTTAAAAGGTAGGTTTTAAAAATAAGTTTTATACATAAAC 1601 AGTTTTGGAGAAAAATTACAGATCATATAAGCAAGACAGTGGCACTAAAATGTTTAATTCATTAATCTGTTTGTTTGGCA 1681 CTGATGCAATGTATGGCTTTTCTCTTGCCCCAAATCACAAACATATGTATCTTTGGGGAAACTAACAATATGATTGCACT 1761 AAATAAACTACTTTGAATAGAGGCCAAATTAATCTTTTAAAAATGATGATAATCATCAGGTTTACTCAGTGAAATCATAT 1841 TAATTATTTTCCAAAATCTAAAAGCTGTAGCTGGAGAAGCCCATGGCCACGAGGAAGCAGCAATTAATTAGATCAACACT 1921 TTTCTCCAGGGTTCACCATGCAGGCAACATTACCTTGTCTTTCAAAAGACACCTGCCTTATGCAAGGGGAAACCTGTGAA 2001 AGCTGCACTCAGAGGGAGGAGTTTTTCTTACATAATTTGCAATTTCAGGAATTTAATTTATAGGCAGATCTTTAAATACA 2081 GTCAACTTACGGTGCACAGTAATATGAAAGCCACACTTTGAAGGTAATAAATACACAGCATGCAGACTGGGAGTTGCTAG 2161 CAAACAAATGGCTTACTTACAAAAGCAGCTTTTAGTTCAGACTTAGTTTTTATAAAATGGGAATTCTGACTTACTTAACC 2241 AGGTTTGGGATGGAGATGGTCTGCATCAGCTTTTTGTATTAACAAAGTTACTGGCTCTTTGTGTGTCTCCAGGTAACTTT 2321 GCTTGATTAAACAGCAAAGCCATATTCTAAATTCACTGTTGAATGCCTGTCCCAGTCCAAATTGTCTGTCTGCTCTTATT 2401 TTTGTACCATATTGCTCTTAAAAATCTTGGTTTGGTACAGTTCATAATTCACCAAAAGTTCATATAATTTAAAGAAACAC 2481 TAAATTAGTTTAAAATGAAGCAATTTATATCTTTATGCAAAAACATATGTCTGTCTTTGCAAAGGACTGTAAGCAGATTA 2561 CAATAAATCCTTTACTTTAATCAAAAAAAAAAAAAAAAAA Target sites Provided by authors Predicted by miRanda |
| miRNA:Target | hsa-miR-124 :: NR3C2 [ Functional MTI ] |
|---|---|
| Validation Method | Luciferase reporter assay , qRT-PCR |
| Conditions | HeLa |
| Location of target site | 3'UTR |
| Tools used in this research | miRanda, PicTar, TargetScan |
| Original Description (Extracted from the article) | ... miR-124 and miR-135a suppress NR3C2//The binding of miR-124 and miR-135a to NR3C2 3'UTR contributes to the translational, not transcriptional regulation of the gene ... - Sober, S. Laan, M. Annilo, T., 2010, Biochem Biophys Res Commun. |
| Article |
- Sober, S.
Laan, M.
Annilo, T. - Biochem Biophys Res Commun, 2010
MicroRNAs (miRNAs) comprise a post-transcriptional layer of gene regulation shown to be involved in diverse physiological processes. We aimed to study whether regulatory networks that determine susceptibility to hypertension may involve a miRNA component. Screening of loci, involved in renal water-salt balance regulation, highlighted the mineralocorticoid receptor gene NR3C2 as a potential target for several miRNAs. A luciferase assay demonstrated that miR-124 and miR-135a suppress NR3C2 3'UTR reporter construct activity 1.5- and 2.2-fold, respectively. As the tested miRNAs did not reduce the levels of target mRNA, we suggest that the binding of miR-124 and miR-135a to NR3C2 3'UTR contributes to the translational, not transcriptional regulation of the gene. Co-expression of two different miRNAs did not increase the repression of the reporter gene, indicating no additive or synergistic effects between the tested miRNAs. Our results demonstrate that by repressing the mineralocorticoid receptor gene NR3C2, miR-124 and miR-135a could participate in the regulation of renin-angiotensin-aldosterone system and thereby might be involved in blood pressure regulation.
LinkOut: [PMID: 19944075]
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