Accession ID: MIRT001209 [miRNA, hsa-miR-494-3p :: PTEN, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-494LinkOut: [miRBase ]
Synonyms MIRN494, hsa-mir-494, MIR494
Description Homo sapiens miR-494 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-494-3p
Mature Sequence 48| UGAAACAUACACGGGAAACCUC |69
Evidence Experimental
Experiments Array-cloned
Putative hsa-miR-494-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol PTEN LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms 10q23del, BZS, DEC, GLM2, MHAM, MMAC1, PTEN1, TEP1
Description phosphatase and tensin homolog
Transcript NM_0003    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on PTEN LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of PTEN
(miRNA target sites are highlighted)
>PTEN|NM_0003|3'UTR
   1 ATTTTTTTTTATCAAGAGGGATAAAACACCATGAAAATAAACTTGAATAAACTGAAAATGGACCTTTTTTTTTTTAATGG
  81 CAATAGGACATTGTGTCAGATTACCAGTTATAGGAACAATTCTCTTTTCCTGACCAATCTTGTTTTACCCTATACATCCA
 161 CAGGGTTTTGACACTTGTTGTCCAGTTGAAAAAAGGTTGTGTAGCTGTGTCATGTATATACCTTTTTGTGTCAAAAGGAC
 241 ATTTAAAATTCAATTAGGATTAATAAAGATGGCACTTTCCCGTTTTATTCCAGTTTTATAAAAAGTGGAGACAGACTGAT
 321 GTGTATACGTAGGAATTTTTTCCTTTTGTGTTCTGTCACCAACTGAAGTGGCTAAAGAGCTTTGTGATATACTGGTTCAC
 401 ATCCTACCCCTTTGCACTTGTGGCAACAGATAAGTTTGCAGTTGGCTAAGAGAGGTTTCCGAAGGGTTTTGCTACATTCT
 481 AATGCATGTATTCGGGTTAGGGGAATGGAGGGAATGCTCAGAAAGGAAATAATTTTATGCTGGACTCTGGACCATATACC
 561 ATCTCCAGCTATTTACACACACCTTTCTTTAGCATGCTACAGTTATTAATCTGGACATTCGAGGAATTGGCCGCTGTCAC
 641 TGCTTGTTGTTTGCGCATTTTTTTTTAAAGCATATTGGTGCTAGAAAAGGCAGCTAAAGGAAGTGAATCTGTATTGGGGT
 721 ACAGGAATGAACCTTCTGCAACATCTTAAGATCCACAAATGAAGGGATATAAAAATAATGTCATAGGTAAGAAACACAGC
 801 AACAATGACTTAACCATATAAATGTGGAGGCTATCAACAAAGAATGGGCTTGAAACATTATAAAAATTGACAATGATTTA
 881 TTAAATATGTTTTCTCAATTGTAACGACTTCTCCATCTCCTGTGTAATCAAGGCCAGTGCTAAAATTCAGATGCTGTTAG
 961 TACCTACATCAGTCAACAACTTACACTTATTTTACTAGTTTTCAATCATAATACCTGCTGTGGATGCTTCATGTGCTGCC
1041 TGCAAGCTTCTTTTTTCTCATTAAATATAAAATATTTTGTAATGCTGCACAGAAATTTTCAATTTGAGATTCTACAGTAA
1121 GCGTTTTTTTTCTTTGAAGATTTATGATGCACTTATTCAATAGCTGTCAGCCGTTCCACCCTTTTGACCTTACACATTCT
1201 ATTACAATGAATTTTGCAGTTTTGCACATTTTTTAAATGTCATTAACTGTTAGGGAATTTTACTTGAATACTGAATACAT
1281 ATAATGTTTATATTAAAAAGGACATTTGTGTTAAAAAGGAAATTAGAGTTGCAGTAAACTTTCAATGCTGCACACAAAAA
1361 AAAGACATTTGATTTTTCAGTAGAAATTGTCCTACATGTGCTTTATTGATTTGCTATTGAAAGAATAGGGTTTTTTTTTT
1441 TTTTTTTTTTTTTTTTTTTAAATGTGCAGTGTTGAATCATTTCTTCATAGTGCTCCCCCGAGTTGGGACTAGGGCTTCAA
1521 TTTCACTTCTTAAAAAAAATCATCATATATTTGATATGCCCAGACTGCATACGATTTTAAGCGGAGTACAACTACTATTG
1601 TAAAGCTAATGTGAAGATATTATTAAAAAGGTTTTTTTTTCCAGAAATTTGGTGTCTTCAAATTATACCTTCACCTTGAC
1681 ATTTGAATATCCAGCCATTTTGTTTCTTAATGGTATAAAATTCCATTTTCAATAACTTATTGGTGCTGAAATTGTTCACT
1761 AGCTGTGGTCTGACCTAGTTAATTTACAAATACAGATTGAATAGGACCTACTAGAGCAGCATTTATAGAGTTTGATGGCA
1841 AATAGATTAGGCAGAACTTCATCTAAAATATTCTTAGTAAATAATGTTGACACGTTTTCCATACCTTGTCAGTTTCATTC
1921 AACAATTTTTAAATTTTTAACAAAGCTCTTAGGATTTACACATTTATATTTAAACATTGATATATAGAGTATTGATTGAT
2001 TGCTCATAAGTTAAATTGGTAAAGTTAGAGACAACTATTCTAACACCTCACCATTGAAATTTATATGCCACCTTGTCTTT
2081 CATAAAAGCTGAAAATTGTTACCTAAAATGAAAATCAACTTCATGTTTTGAAGATAGTTATAAATATTGTTCTTTGTTAC
2161 AATTTCGGGCACCGCATATTAAAACGTAACTTTATTGTTCCAATATGTAACATGGAGGGCCAGGTCATAAATAATGACAT
2241 TATAATGGGCTTTTGCACTGTTATTATTTTTCCTTTGGAATGTGAAGGTCTGAATGAGGGTTTTGATTTTGAATGTTTCA
2321 ATGTTTTTGAGAAGCCTTGCTTACATTTTATGGTGTAGTCATTGGAAATGGAAAAATGGCATTATATATATTATATATAT
2401 AAATATATATTATACATACTCTCCTTACTTTATTTCAGTTACCATCCCCATAGAATTTGACAAGAATTGCTATGACTGAA
2481 AGGTTTTCGAGTCCTAATTAAAACTTTATTTATGGCAGTATTCATAATTAGCCTGAAATGCATTCTGTAGGTAATCTCTG
2561 AGTTTCTGGAATATTTTCTTAGACTTTTTGGATGTGCAGCAGCTTACATGTCTGAAGTTACTTGAAGGCATCACTTTTAA
2641 GAAAGCTTACAGTTGGGCCCTGTACCATCCCAAGTCCTTTGTAGCTCCTCTTGAACATGTTTGCCATACTTTTAAAAGGG
2721 TAGTTGAATAAATAGCATCACCATTCTTTGCTGTGGCACAGGTTATAAACTTAAGTGGAGTTTACCGGCAGCATCAAATG
2801 TTTCAGCTTTAAAAAATAAAAGTAGGGTACAAGTTTAATGTTTAGTTCTAGAAATTTTGTGCAATATGTTCATAACGATG
2881 GCTGTGGTTGCCACAAAGTGCCTCGTTTACCTTTAAATACTGTTAATGTGTCATGCATGCAGATGGAAGGGGTGGAACTG
2961 TGCACTAAAGTGGGGGCTTTAACTGTAGTATTTGGCAGAGTTGCCTTCTACCTGCCAGTTCAAAAGTTCAACCTGTTTTC
3041 ATATAGAATATATATACTAAAAAATTTCAGTCTGTTAAACAGCCTTACTCTGATTCAGCCTCTTCAGATACTCTTGTGCT
3121 GTGCAGCAGTGGCTCTGTGTGTAAATGCTATGCACTGAGGATACACAAAAATACCAATATGATGTGTACAGGATAATGCC
3201 TCATCCCAATCAGATGTCCATTTGTTATTGTGTTTGTTAACAACCCTTTATCTCTTAGTGTTATAAACTCCACTTAAAAC
3281 TGATTAAAGTCTCATTCTTGTCA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' cuCCAAAG--GGCACAUACAAAGu 5'
            |||||:  :: || ||||||| 
Target 5' agGGTTTTGATTTTGAATGTTTCa 3'
2297 - 2320 162.00 -12.60
2
miRNA  3' cucCAAAGGGC--AC--A---UACAAAGu 5'
             |||| |||   |  |   ||||||| 
Target 5' ggaGTTTACCGGCAGCATCAAATGTTTCa 3'
2777 - 2805 147.00 -12.62
3
miRNA  3' cuCCAAAGGGCACA-UACAAAgu 5'
            || | |  || | ||||||  
Target 5' taGGGTACAAGTTTAATGTTTag 3'
2823 - 2845 132.00 -7.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-494-3p :: PTEN    [ Functional MTI ]
Validation Method qRT-PCR , Luciferase reporter assay , Western blot ,
Conditions 16HBE-T30
Location of target site 3'UTR
Tools used in this research TargetScan , PicTar
Original Description (Extracted from the article) ... In 16HBE-T30 cells, while co-transfection with pre-miR-494 resulted in a signi´Čücant decrease in luciferase/renilla activity ...

- Liu, L. Jiang, Y. Zhang, H. Greenlee, A. R. et al., 2010, Life Sci.

Article - Liu, L. Jiang, Y. Zhang, H. Greenlee, A. R. et al.
- Life Sci, 2010
AIMS: We investigated the functionality of miR-494 in anti-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (anti-BPDE)-transformed human bronchial epithelial cell 16HBE to reveal its potential target coding-gene. MAIN METHODS: The expression of mature miR-494 in cells was detected by miRNA-specific quantitative real-time polymerase chain reaction (QRT-PCR). QRT-PCR and Western blot were used to identify the expression of phosphatase and tensin homolog (PTEN) mRNA and protein. Following activation or inhibition of mature miRNA expression with precursors or antisense inhibitors, PTEN expression, luciferase activities, cell apoptosis, cell growth in soft agar and cell motility were analyzed. KEY FINDINGS: The expression of miR-494 increased while PTEN protein appeared to be lower in malignant transformed 16HBE cells. Enforced miR-494 level decreased PTEN protein expression compared to a negative precursor control group. Inhibition of miR-494 expression increased PTEN protein expression compared to negative inhibitor control group. Decreased expression of miR-494 increased caspase-3/7 activities in transformed 16HBE cells, and increased expression of miR-494 decreased this activity. Inhibition of miR-494 also decreased the malignancy of transformed cells. SIGNIFICANCE: MiR-494 regulates the expression of PTEN post-transcriptionally and functions as a micro-oncogene in carcinogenesis induced by anti-BPDE. MiR-494 may be a useful target for gene therapy.
LinkOut: [PMID: 20006626]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-494-3p :: PTEN    [ Functional MTI ]
Validation Method Flow , Luciferase reporter assay , Microarray , qRT-PCR , Western blot
Conditions HEK293T
Location of target site 3'UTR
Tools used in this research miRTar , PicTar , TargetScan
Original Description (Extracted from the article) ... PTEN is a functional target of miR-494, and the PTEN/Akt pathway is critical for the activation of MDSCs ...

- Liu, Y. Lai, L. Chen, Q. Song, Y. Xu, S. et al., 2012, J Immunol.

Article - Liu, Y. Lai, L. Chen, Q. Song, Y. Xu, S. et al.
- J Immunol, 2012
Myeloid-derived suppressor cells (MDSCs) potently suppress the anti-tumor immune responses and also orchestrate the tumor microenvironment that favors tumor angiogenesis and metastasis. The molecular networks regulating the accumulation and functions of tumor-expanded MDSCs are largely unknown. In this study, we identified microRNA-494 (miR-494), whose expression was dramatically induced by tumor-derived factors, as an essential player in regulating the accumulation and activity of MDSCs by targeting of phosphatase and tensin homolog (PTEN) and activation of the Akt pathway. TGF-beta1 was found to be the main tumor-derived factor responsible for the upregulation of miR-494 in MDSCs. Expression of miR-494 not only enhanced CXCR4-mediated MDSC chemotaxis but also altered the intrinsic apoptotic/survival signal by targeting of PTEN, thus contributing to the accumulation of MDSCs in tumor tissues. Consequently, downregulation of PTEN resulted in increased activity of the Akt pathway and the subsequent upregulation of MMPs for facilitation of tumor cell invasion and metastasis. Knockdown of miR-494 significantly reversed the activity of MDSCs and inhibited the tumor growth and metastasis of 4T1 murine breast cancer in vivo. Collectively, our findings reveal that TGF-beta1-induced miR-494 expression in MDSCs plays a critical role in the molecular events governing the accumulation and functions of tumor-expanded MDSCs and might be identified as a potential target in cancer therapy.
LinkOut: [PMID: 22544933]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
4 hsa-miR-494-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001209 PTEN phosphatase and tensin homolog 5 2
MIRT006112 CDK6 cyclin-dependent kinase 6 4 1
MIRT006653 ARNTL aryl hydrocarbon receptor nuclear translocator-like 2 1
MIRT006981 BCL2L11 BCL2-like 11 (apoptosis facilitator) 3 1