Accession ID: MIRT001209 [miRNA, hsa-miR-494-3p :: PTEN, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-494LinkOut: [miRBase ]
Synonyms MIRN494, hsa-mir-494, MIR494
Description Homo sapiens miR-494 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-494-3p
Mature Sequence 48| UGAAACAUACACGGGAAACCUC |69
Evidence Experimental
Experiments Array-cloned
Putative hsa-miR-494-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol PTEN LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms 10q23del, BZS, DEC, GLM2, MHAM, MMAC1, PTEN1, TEP1
Description phosphatase and tensin homolog
Transcript NM_000314    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on PTEN LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of PTEN
(miRNA target sites are highlighted)
>PTEN|NM_000314|3'UTR
   1 ATTTTTTTTTATCAAGAGGGATAAAACACCATGAAAATAAACTTGAATAAACTGAAAATGGACCTTTTTTTTTTTAATGG
  81 CAATAGGACATTGTGTCAGATTACCAGTTATAGGAACAATTCTCTTTTCCTGACCAATCTTGTTTTACCCTATACATCCA
 161 CAGGGTTTTGACACTTGTTGTCCAGTTGAAAAAAGGTTGTGTAGCTGTGTCATGTATATACCTTTTTGTGTCAAAAGGAC
 241 ATTTAAAATTCAATTAGGATTAATAAAGATGGCACTTTCCCGTTTTATTCCAGTTTTATAAAAAGTGGAGACAGACTGAT
 321 GTGTATACGTAGGAATTTTTTCCTTTTGTGTTCTGTCACCAACTGAAGTGGCTAAAGAGCTTTGTGATATACTGGTTCAC
 401 ATCCTACCCCTTTGCACTTGTGGCAACAGATAAGTTTGCAGTTGGCTAAGAGAGGTTTCCGAAGGGTTTTGCTACATTCT
 481 AATGCATGTATTCGGGTTAGGGGAATGGAGGGAATGCTCAGAAAGGAAATAATTTTATGCTGGACTCTGGACCATATACC
 561 ATCTCCAGCTATTTACACACACCTTTCTTTAGCATGCTACAGTTATTAATCTGGACATTCGAGGAATTGGCCGCTGTCAC
 641 TGCTTGTTGTTTGCGCATTTTTTTTTAAAGCATATTGGTGCTAGAAAAGGCAGCTAAAGGAAGTGAATCTGTATTGGGGT
 721 ACAGGAATGAACCTTCTGCAACATCTTAAGATCCACAAATGAAGGGATATAAAAATAATGTCATAGGTAAGAAACACAGC
 801 AACAATGACTTAACCATATAAATGTGGAGGCTATCAACAAAGAATGGGCTTGAAACATTATAAAAATTGACAATGATTTA
 881 TTAAATATGTTTTCTCAATTGTAACGACTTCTCCATCTCCTGTGTAATCAAGGCCAGTGCTAAAATTCAGATGCTGTTAG
 961 TACCTACATCAGTCAACAACTTACACTTATTTTACTAGTTTTCAATCATAATACCTGCTGTGGATGCTTCATGTGCTGCC
1041 TGCAAGCTTCTTTTTTCTCATTAAATATAAAATATTTTGTAATGCTGCACAGAAATTTTCAATTTGAGATTCTACAGTAA
1121 GCGTTTTTTTTCTTTGAAGATTTATGATGCACTTATTCAATAGCTGTCAGCCGTTCCACCCTTTTGACCTTACACATTCT
1201 ATTACAATGAATTTTGCAGTTTTGCACATTTTTTAAATGTCATTAACTGTTAGGGAATTTTACTTGAATACTGAATACAT
1281 ATAATGTTTATATTAAAAAGGACATTTGTGTTAAAAAGGAAATTAGAGTTGCAGTAAACTTTCAATGCTGCACACAAAAA
1361 AAAGACATTTGATTTTTCAGTAGAAATTGTCCTACATGTGCTTTATTGATTTGCTATTGAAAGAATAGGGTTTTTTTTTT
1441 TTTTTTTTTTTTTTTTTTTAAATGTGCAGTGTTGAATCATTTCTTCATAGTGCTCCCCCGAGTTGGGACTAGGGCTTCAA
1521 TTTCACTTCTTAAAAAAAATCATCATATATTTGATATGCCCAGACTGCATACGATTTTAAGCGGAGTACAACTACTATTG
1601 TAAAGCTAATGTGAAGATATTATTAAAAAGGTTTTTTTTTCCAGAAATTTGGTGTCTTCAAATTATACCTTCACCTTGAC
1681 ATTTGAATATCCAGCCATTTTGTTTCTTAATGGTATAAAATTCCATTTTCAATAACTTATTGGTGCTGAAATTGTTCACT
1761 AGCTGTGGTCTGACCTAGTTAATTTACAAATACAGATTGAATAGGACCTACTAGAGCAGCATTTATAGAGTTTGATGGCA
1841 AATAGATTAGGCAGAACTTCATCTAAAATATTCTTAGTAAATAATGTTGACACGTTTTCCATACCTTGTCAGTTTCATTC
1921 AACAATTTTTAAATTTTTAACAAAGCTCTTAGGATTTACACATTTATATTTAAACATTGATATATAGAGTATTGATTGAT
2001 TGCTCATAAGTTAAATTGGTAAAGTTAGAGACAACTATTCTAACACCTCACCATTGAAATTTATATGCCACCTTGTCTTT
2081 CATAAAAGCTGAAAATTGTTACCTAAAATGAAAATCAACTTCATGTTTTGAAGATAGTTATAAATATTGTTCTTTGTTAC
2161 AATTTCGGGCACCGCATATTAAAACGTAACTTTATTGTTCCAATATGTAACATGGAGGGCCAGGTCATAAATAATGACAT
2241 TATAATGGGCTTTTGCACTGTTATTATTTTTCCTTTGGAATGTGAAGGTCTGAATGAGGGTTTTGATTTTGAATGTTTCA
2321 ATGTTTTTGAGAAGCCTTGCTTACATTTTATGGTGTAGTCATTGGAAATGGAAAAATGGCATTATATATATTATATATAT
2401 AAATATATATTATACATACTCTCCTTACTTTATTTCAGTTACCATCCCCATAGAATTTGACAAGAATTGCTATGACTGAA
2481 AGGTTTTCGAGTCCTAATTAAAACTTTATTTATGGCAGTATTCATAATTAGCCTGAAATGCATTCTGTAGGTAATCTCTG
2561 AGTTTCTGGAATATTTTCTTAGACTTTTTGGATGTGCAGCAGCTTACATGTCTGAAGTTACTTGAAGGCATCACTTTTAA
2641 GAAAGCTTACAGTTGGGCCCTGTACCATCCCAAGTCCTTTGTAGCTCCTCTTGAACATGTTTGCCATACTTTTAAAAGGG
2721 TAGTTGAATAAATAGCATCACCATTCTTTGCTGTGGCACAGGTTATAAACTTAAGTGGAGTTTACCGGCAGCATCAAATG
2801 TTTCAGCTTTAAAAAATAAAAGTAGGGTACAAGTTTAATGTTTAGTTCTAGAAATTTTGTGCAATATGTTCATAACGATG
2881 GCTGTGGTTGCCACAAAGTGCCTCGTTTACCTTTAAATACTGTTAATGTGTCATGCATGCAGATGGAAGGGGTGGAACTG
2961 TGCACTAAAGTGGGGGCTTTAACTGTAGTATTTGGCAGAGTTGCCTTCTACCTGCCAGTTCAAAAGTTCAACCTGTTTTC
3041 ATATAGAATATATATACTAAAAAATTTCAGTCTGTTAAACAGCCTTACTCTGATTCAGCCTCTTCAGATACTCTTGTGCT
3121 GTGCAGCAGTGGCTCTGTGTGTAAATGCTATGCACTGAGGATACACAAAAATACCAATATGATGTGTACAGGATAATGCC
3201 TCATCCCAATCAGATGTCCATTTGTTATTGTGTTTGTTAACAACCCTTTATCTCTTAGTGTTATAAACTCCACTTAAAAC
3281 TGATTAAAGTCTCATTCTTGTCA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' cuCCAAAG--GGCACAUACAAAGu 5'
            |||||:  :: || ||||||| 
Target 5' agGGTTTTGATTTTGAATGTTTCa 3'
2297 - 2320 162.00 -12.60
2
miRNA  3' cucCAAAGGGC--AC--A---UACAAAGu 5'
             |||| |||   |  |   ||||||| 
Target 5' ggaGTTTACCGGCAGCATCAAATGTTTCa 3'
2777 - 2805 147.00 -12.62
3
miRNA  3' cuCCAAAGGGCACA-UACAAAgu 5'
            || | |  || | ||||||  
Target 5' taGGGTACAAGTTTAATGTTTag 3'
2823 - 2845 132.00 -7.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-494-3p :: PTEN    [ Functional MTI ]
Validation Method qRT-PCR , Luciferase reporter assay , Western blot ,
Conditions 16HBE-T30
Location of target site 3'UTR
Tools used in this research TargetScan , PicTar
Original Description (Extracted from the article) ... In 16HBE-T30 cells, while co-transfection with pre-miR-494 resulted in a signi´Čücant decrease in luciferase/renilla activity ...

- Liu, L. Jiang, Y. Zhang, H. Greenlee, A. R. et al., 2010, Life Sci.

Article - Liu, L. Jiang, Y. Zhang, H. Greenlee, A. R. et al.
- Life Sci, 2010
AIMS: We investigated the functionality of miR-494 in anti-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (anti-BPDE)-transformed human bronchial epithelial cell 16HBE to reveal its potential target coding-gene. MAIN METHODS: The expression of mature miR-494 in cells was detected by miRNA-specific quantitative real-time polymerase chain reaction (QRT-PCR). QRT-PCR and Western blot were used to identify the expression of phosphatase and tensin homolog (PTEN) mRNA and protein. Following activation or inhibition of mature miRNA expression with precursors or antisense inhibitors, PTEN expression, luciferase activities, cell apoptosis, cell growth in soft agar and cell motility were analyzed. KEY FINDINGS: The expression of miR-494 increased while PTEN protein appeared to be lower in malignant transformed 16HBE cells. Enforced miR-494 level decreased PTEN protein expression compared to a negative precursor control group. Inhibition of miR-494 expression increased PTEN protein expression compared to negative inhibitor control group. Decreased expression of miR-494 increased caspase-3/7 activities in transformed 16HBE cells, and increased expression of miR-494 decreased this activity. Inhibition of miR-494 also decreased the malignancy of transformed cells. SIGNIFICANCE: MiR-494 regulates the expression of PTEN post-transcriptionally and functions as a micro-oncogene in carcinogenesis induced by anti-BPDE. MiR-494 may be a useful target for gene therapy.
LinkOut: [PMID: 20006626]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-494-3p :: PTEN    [ Functional MTI ]
Validation Method Flow , Luciferase reporter assay , Microarray , qRT-PCR , Western blot
Conditions HEK293T
Location of target site 3'UTR
Tools used in this research miRTar , PicTar , TargetScan
Original Description (Extracted from the article) ... PTEN is a functional target of miR-494, and the PTEN/Akt pathway is critical for the activation of MDSCs ...

- Liu, Y. Lai, L. Chen, Q. Song, Y. Xu, S. et al., 2012, J Immunol.

Article - Liu, Y. Lai, L. Chen, Q. Song, Y. Xu, S. et al.
- J Immunol, 2012
Myeloid-derived suppressor cells (MDSCs) potently suppress the anti-tumor immune responses and also orchestrate the tumor microenvironment that favors tumor angiogenesis and metastasis. The molecular networks regulating the accumulation and functions of tumor-expanded MDSCs are largely unknown. In this study, we identified microRNA-494 (miR-494), whose expression was dramatically induced by tumor-derived factors, as an essential player in regulating the accumulation and activity of MDSCs by targeting of phosphatase and tensin homolog (PTEN) and activation of the Akt pathway. TGF-beta1 was found to be the main tumor-derived factor responsible for the upregulation of miR-494 in MDSCs. Expression of miR-494 not only enhanced CXCR4-mediated MDSC chemotaxis but also altered the intrinsic apoptotic/survival signal by targeting of PTEN, thus contributing to the accumulation of MDSCs in tumor tissues. Consequently, downregulation of PTEN resulted in increased activity of the Akt pathway and the subsequent upregulation of MMPs for facilitation of tumor cell invasion and metastasis. Knockdown of miR-494 significantly reversed the activity of MDSCs and inhibited the tumor growth and metastasis of 4T1 murine breast cancer in vivo. Collectively, our findings reveal that TGF-beta1-induced miR-494 expression in MDSCs plays a critical role in the molecular events governing the accumulation and functions of tumor-expanded MDSCs and might be identified as a potential target in cancer therapy.
LinkOut: [PMID: 22544933]
MiRNA-Target Expression Profile:

 
MiRNA-Target Expression Profile(TCGA):

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
126 hsa-miR-494-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001209 PTEN phosphatase and tensin homolog 5 2
MIRT006112 CDK6 cyclin-dependent kinase 6 4 1
MIRT006653 ARNTL aryl hydrocarbon receptor nuclear translocator-like 2 1
MIRT006981 BCL2L11 BCL2-like 11 (apoptosis facilitator) 3 1
MIRT053227 ATF3 activating transcription factor 3 2 1
MIRT053346 SDC1 syndecan 1 2 1
MIRT053355 PROS1 protein S (alpha) 3 1
MIRT053512 BMI1 BMI1 polycomb ring finger oncogene 4 1
MIRT053515 ZEB1 zinc finger E-box binding homeobox 1 4 1
MIRT053755 BAG1 BCL2-associated athanogene 4 1
MIRT053756 MYC v-myc myelocytomatosis viral oncogene homolog (avian) 4 2
MIRT054314 TFAM transcription factor A, mitochondrial 3 1
MIRT054315 FOXJ3 forkhead box J3 3 1
MIRT054719 HNRNPA3 heterogeneous nuclear ribonucleoprotein A3 2 1
MIRT054720 PDIA3 protein disulfide isomerase family A, member 3 2 1
MIRT054721 RAD23B RAD23 homolog B (S. cerevisiae) 3 2
MIRT054722 SYNCRIP synaptotagmin binding, cytoplasmic RNA interacting protein 2 1
MIRT078392 DCAF7 DDB1 and CUL4 associated factor 7 1 2
MIRT079869 DSG2 desmoglein 2 1 2
MIRT138434 KIF2C kinesin family member 2C 1 1
MIRT274621 RAP1B RAP1B, member of RAS oncogene family 1 1
MIRT334166 CCND1 cyclin D1 1 2
MIRT438172 ARHGAP5 Rho GTPase activating protein 5 3 1
MIRT438243 MCC mutated in colorectal cancers 3 1
MIRT438441 CNR1 cannabinoid receptor 1 (brain) 2 1
MIRT438475 SLC26A3 solute carrier family 26, member 3 1 1
MIRT438701 CXCR4 chemokine (C-X-C motif) receptor 4 3 1
MIRT438770 MAP2K1 mitogen-activated protein kinase kinase 1 2 1
MIRT438771 ITPR1 inositol 1,4,5-trisphosphate receptor, type 1 2 1
MIRT438772 BCL2 B-cell CLL/lymphoma 2 2 1
MIRT438884 CFTR cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) 2 1
MIRT447204 PCNP PEST proteolytic signal containing nuclear protein 1 2
MIRT448177 IL1RAP interleukin 1 receptor accessory protein 1 2
MIRT448639 NPNT nephronectin 1 1
MIRT449507 TM6SF1 transmembrane 6 superfamily member 1 1 1
MIRT449820 FNBP1 formin binding protein 1 1 1
MIRT462020 RIF1 RAP1 interacting factor homolog (yeast) 1 1
MIRT463431 ZC3HAV1L zinc finger CCCH-type, antiviral 1-like 1 1
MIRT464428 UHMK1 U2AF homology motif (UHM) kinase 1 1 1
MIRT467503 SMIM13 chromosome 6 open reading frame 228 1 1
MIRT468550 SERP1 stress-associated endoplasmic reticulum protein 1 1 1
MIRT468958 RPS14 ribosomal protein S14 1 2
MIRT469273 RHOB ras homolog family member B 1 4
MIRT469304 RGS16 regulator of G-protein signaling 16 1 1
MIRT473437 MDM4 Mdm4 p53 binding protein homolog (mouse) 1 1
MIRT474272 LATS2 LATS, large tumor suppressor, homolog 2 (Drosophila) 1 1
MIRT478444 DAZAP2 DAZ associated protein 2 1 1
MIRT481062 BASP1 brain abundant, membrane attached signal protein 1 1 1
MIRT485272 PLEKHA3 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 3 1 1
MIRT485782 ATXN1 ataxin 1 1 1
MIRT493935 ETF1 eukaryotic translation termination factor 1 1 2
MIRT497355 AJAP1 adherens junctions associated protein 1 1 1
MIRT497731 ZNF620 zinc finger protein 620 1 1
MIRT498070 SLC25A12 solute carrier family 25 (aspartate/glutamate carrier), member 12 1 1
MIRT498807 CDS2 CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2 1 2
MIRT505321 TMOD3 tropomodulin 3 (ubiquitous) 1 2
MIRT505642 SHOC2 soc-2 suppressor of clear homolog (C. elegans) 1 1
MIRT505916 REST RE1-silencing transcription factor 1 1
MIRT507719 CMPK1 cytidine monophosphate (UMP-CMP) kinase 1, cytosolic 1 3
MIRT511011 PABPN1 poly(A) binding protein, nuclear 1 1 1
MIRT511436 HOXA10 homeobox A10 1 3
MIRT512603 EFCAB1 EF-hand calcium binding domain 1 1 2
MIRT519035 AGPS alkylglycerone phosphate synthase 1 2
MIRT521291 RRAGD Ras-related GTP binding D 1 2
MIRT523845 EVI5 ecotropic viral integration site 5 1 3
MIRT524826 ARSJ arylsulfatase family, member J 1 1
MIRT527609 EYS eyes shut homolog (Drosophila) 1 1
MIRT529617 ZSCAN16 zinc finger and SCAN domain containing 16 1 1
MIRT530177 ZBED2 zinc finger, BED-type containing 2 1 1
MIRT531446 HSD17B12 hydroxysteroid (17-beta) dehydrogenase 12 1 1
MIRT532327 INSL4 insulin-like 4 (placenta) 1 1
MIRT532880 ZNF532 zinc finger protein 532 1 1
MIRT532952 ZNF24 zinc finger protein 24 1 2
MIRT533707 TMEM64 transmembrane protein 64 1 1
MIRT533858 TEAD1 TEA domain family member 1 (SV40 transcriptional enhancer factor) 1 1
MIRT534168 SLC8A1 solute carrier family 8 (sodium/calcium exchanger), member 1 1 1
MIRT535903 MKNK2 MAP kinase interacting serine/threonine kinase 2 1 1
MIRT536716 IGF1R insulin-like growth factor 1 receptor 1 1
MIRT537477 FAM63B family with sequence similarity 63, member B 1 1
MIRT538539 CEP97 centrosomal protein 97kDa 1 1
MIRT541280 GLO1 glyoxalase I 1 1
MIRT544876 ZNF543 zinc finger protein 543 1 1
MIRT545256 TRIM36 tripartite motif containing 36 1 2
MIRT546840 RAB4A RAB4A, member RAS oncogene family 1 1
MIRT547313 NPTN neuroplastin 1 1
MIRT548148 FYCO1 FYVE and coiled-coil domain containing 1 1 1
MIRT554907 RB1 retinoblastoma 1 1 1
MIRT555582 PITX2 paired-like homeodomain 2 1 1
MIRT555693 PEX11B peroxisomal biogenesis factor 11 beta 1 1
MIRT556664 KMT2D myeloid/lymphoid or mixed-lineage leukemia 2 1 2
MIRT561490 SUPT16H suppressor of Ty 16 homolog (S. cerevisiae) 1 1
MIRT561863 NCL nucleolin 1 1
MIRT565007 WDR45B WDR45-like 1 1
MIRT566190 PTPN14 protein tyrosine phosphatase, non-receptor type 14 1 1
MIRT569839 DCAF4L2 DDB1 and CUL4 associated factor 4-like 2 1 1
MIRT571665 NHS Nance-Horan syndrome (congenital cataracts and dental anomalies) 1 1
MIRT572524 ZNF736 zinc finger protein 736 1 1
MIRT607095 ARHGAP12 Rho GTPase activating protein 12 1 2
MIRT609279 MAPKAPK5 mitogen-activated protein kinase-activated protein kinase 5 1 1
MIRT609450 CCDC149 coiled-coil domain containing 149 1 1
MIRT622064 SS18 synovial sarcoma translocation, chromosome 18 1 1
MIRT623315 MAPK1 mitogen-activated protein kinase 1 1 1
MIRT636008 SAMD15 sterile alpha motif domain containing 15 1 1
MIRT636208 SPATA13 spermatogenesis associated 13 1 1
MIRT639956 PRRC2B proline-rich coiled-coil 2B 1 1
MIRT644507 GTF2F1 general transcription factor IIF, polypeptide 1, 74kDa 1 1
MIRT650763 WNT16 wingless-type MMTV integration site family, member 16 1 1
MIRT652492 TMEM178B transmembrane protein 178B 1 1
MIRT654824 PPP1CC protein phosphatase 1, catalytic subunit, gamma isozyme 1 1
MIRT657182 INO80C INO80 complex subunit C 1 1
MIRT659378 CREG2 cellular repressor of E1A-stimulated genes 2 1 1
MIRT659973 C2CD4A C2 calcium-dependent domain containing 4A 1 1
MIRT664208 TBCA tubulin folding cofactor A 1 1
MIRT684318 GTF3C4 general transcription factor IIIC, polypeptide 4, 90kDa 1 1
MIRT687147 PTPN12 protein tyrosine phosphatase, non-receptor type 12 1 1
MIRT700620 PRKAA2 protein kinase, AMP-activated, alpha 2 catalytic subunit 1 1
MIRT702964 HIF1A hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) 1 1
MIRT712937 GALP galanin-like peptide 1 1
MIRT713181 ERO1L ERO1-like (S. cerevisiae) 1 1
MIRT714474 CYCS cytochrome c, somatic 1 1
MIRT715659 ZBTB25 zinc finger and BTB domain containing 25 1 1
MIRT717493 RNF115 ring finger protein 115 1 1
MIRT720797 GCH1 GTP cyclohydrolase 1 1 1
MIRT721790 TMEFF2 transmembrane protein with EGF-like and two follistatin-like domains 2 1 1
MIRT723245 TMLHE trimethyllysine hydroxylase, epsilon 1 1
MIRT725536 EN2 engrailed homeobox 2 1 1
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