Accession ID: MIRT001557 [miRNA, hsa-miR-155 :: CTNNB1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-155 LinkOut: [miRBase ]
Description Homo sapiens miR-155 stem-loop
Comment Human mir-155 is predicted based on homology to a cloned miR from mouse (MIR:MI0000177) .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-155-3p
Mature Sequence 43| CUCCUACAUAUUAGCAUUAACA |64
Evidence Experimental
Experiments Cloned
Putative hsa-miR-155-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-155-5p
Mature Sequence 4| UUAAUGCUAAUCGUGAUAGGGGU |26
Evidence Experimental
Experiments Cloned
Putative hsa-miR-155-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol CTNNB1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms CTNNB, DKFZp686D02253, FLJ25606, FLJ37923
Description catenin (cadherin-associated protein), beta 1, 88kDa
Transcript NM_001904   LinkOut: [ RefSeq ]
Other Transcripts NM_001098210, NM_001098209   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04310    Wnt signaling pathway - Homo sapiens (human)
hsa04510    Focal adhesion - Homo sapiens (human)
hsa04520    Adherens junction - Homo sapiens (human)
hsa04530    Tight junction - Homo sapiens (human)
hsa04670    Leukocyte transendothelial migration - Homo sapiens (human)
hsa04916    Melanogenesis - Homo sapiens (human)
hsa05100    Bacterial invasion of epithelial cells - Homo sapiens (human)
hsa05130    Pathogenic Escherichia coli infection - Homo sapiens (human)
hsa05200    Pathways in cancer - Homo sapiens (human)
hsa05210    Colorectal cancer - Homo sapiens (human)
hsa05213    Endometrial cancer - Homo sapiens (human)
hsa05215    Prostate cancer - Homo sapiens (human)
hsa05216    Thyroid cancer - Homo sapiens (human)
hsa05217    Basal cell carcinoma - Homo sapiens (human)
hsa05412    Arrhythmogenic right ventricular cardiomyopathy (ARVC) - Homo sapiens (human)
Putative miRNA Targets on CTNNB1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of CTNNB1
(miRNA target sites are highlighted)		 
>CTNNB1|NM_001904|3'UTR
   1 TAAATCATCCTTTAGGTAAGAAGTTTTAAAAAGCCAGTTTGGGTAAAATACTTTTACTCTGCCTACAGAACTTCAGAAAG
  81 ACTTGGTTGGTAGGGTGGGAGTGGTTTAGGCTATTTGTAAATCTGCCACAAAAACAGGTATATACTTTGAAAGGAGATGT
 161 CTTGGAACATTGGAATGTTCTCAGATTTCTGGTTGTTATGTGATCATGTGTGGAAGTTATTAACTTTAATGTTTTTTGCC
 241 ACAGCTTTTGCAACTTAATACTCAAATGAGTAACATTTGCTGTTTTAAACATTAATAGCAGCCTTTCTCTCTTTATACAG
 321 CTGTATTGTCTGAACTTGCATTGTGATTGGCCTGTAGAGTTGCTGAGAGGGCTCGAGGGGTGGGCTGGTATCTCAGAAAG
 401 TGCCTGACACACTAACCAAGCTGAGTTTCCTATGGGAACAATTGAAGTAAACTTTTTGTTCTGGTCCTTTTTGGTCGAGG
 481 AGTAACAATACAAATGGATTTTGGGAGTGACTCAAGAAGTGAAGAATGCACAAGAATGGATCACAAGATGGAATTTATCA
 561 AACCCTAGCCTTGCTTGTTAAATTTTTTTTTTTTTTTTTTTAAGAATATCTGTAATGGTACTGACTTTGCTTGCTTTGAA
 641 GTAGCTCTTTTTTTTTTTTTTTTTTTTTTTTTGCAGTAACTGTTTTTTAAGTCTCTCGTAGTGTTAAGTTATAGTGAATA
 721 CTGCTACAGCAATTTCTAATTTTTAAGAATTGAGTAATGGTGTAGAACACTAATTCATAATCACTCTAATTAATTGTAAT
 801 CTGAATAAAGTGTAACAATTGTGTAGCCTTTTTGTATAAAATAGACAAATAGAAAATGGTCCAATTAGTTTCCTTTTTAA
 881 TATGCTTAAAATAAGCAGGTGGATCTATTTCATGTTTTTGATCAAAAACTATTTGGGATATGTATGGGTAGGGTAAATCA
 961 GTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTTTTATCCCAAAGTTGTTGTAACCTGCTGTGA
1041 TACGATGCTTCAAGAGAAAATGCGGTTATAAAAAATGGTTCAGAATTAAACTTTTAATTCATTCGATTG
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' acAAU-UACGAUUAUACAUCCUc 5'
            ||| :|| | ||:|||:| | 
Target 5' tgTTATGTGATCATGTGTGGAAg 3'
 		194 - 216 120.00 -12.80
2 
miRNA  3' acaAUUACG-AUU----AUACAUCcuc 5'
             ||| |: |||    |:|||||   
Target 5' gaaTAAAGTGTAACAATTGTGTAGcct 3'
 		803 - 829 117.00 -6.22
3 
miRNA  3' acaaUUACGAUUAU---ACAUCCUc 5'
              ||| | |||:   | |:||| 
Target 5' taacAATACAAATGGATTTTGGGAg 3'
 		483 - 507 110.00 -7.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-155 :: CTNNB1    [ Functional MTI ]
Validation Method pSILAC
Conditions HeLa
Original Description (Extracted from the article) ... Provided by pSILAC database (http://psilac.mdc-berlin.de/) ...

- Selbach, M. Schwanhausser, B. Thierfelder, et al., 2008, Nature.
Article - Selbach, M. Schwanhausser, B. Thierfelder, et al.
- Nature, 2008
Animal microRNAs (miRNAs) regulate gene expression by inhibiting translation and/or by inducing degradation of target messenger RNAs. It is unknown how much translational control is exerted by miRNAs on a genome-wide scale. We used a new proteomic approach to measure changes in synthesis of several thousand proteins in response to miRNA transfection or endogenous miRNA knockdown. In parallel, we quantified mRNA levels using microarrays. Here we show that a single miRNA can repress the production of hundreds of proteins, but that this repression is typically relatively mild. A number of known features of the miRNA-binding site such as the seed sequence also govern repression of human protein synthesis, and we report additional target sequence characteristics. We demonstrate that, in addition to downregulating mRNA levels, miRNAs also directly repress translation of hundreds of genes. Finally, our data suggest that a miRNA can, by direct or indirect effects, tune protein synthesis from thousands of genes.
LinkOut: [PMID: 18668040]