Accession ID: MIRT001596 [miRNA, hsa-let-7b :: SCYL1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-let-7b LinkOut: [miRBase ]
Synonyms LET7B, MIRNLET7B, hsa-let-7b, let-7b, MIRLET7B
Description Homo sapiens let-7b stem-loop
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-let-7b-3p
Mature Sequence 60| CUAUACAACCUACUGCCUUCCC |81
Evidence Experimental
Experiments Cloned
Putative hsa-let-7b-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-let-7b-5p
Mature Sequence 6| UGAGGUAGUAGGUUGUGUGGUU |27
Evidence Experimental
Experiments Cloned
Putative hsa-let-7b-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol SCYL1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms GKLP, MGC78454, NKTL, NTKL, P105, TAPK, TEIF, TRAP
Description SCY1-like 1 (S. cerevisiae)
Transcript NM_001048218   LinkOut: [ RefSeq ]
Other Transcripts NM_020680   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on SCYL1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of SCYL1
(miRNA target sites are highlighted)		 
>SCYL1|NM_001048218|3'UTR
   1 TGAACCGTGGCGGTGGCCCTTCCCGGCTGCGGAGAGCCCGCCCCACAGATGTATTTATTGTACAAACCATGTGAGCCCGG
  81 CCGGCCCAGCCAGGCCATCTCACGTGTACATAATCAGAGCCACAATAAATTCTATTTCACACCCCTTGAAAAAAAAAAAA
 161 AAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' ccCUU----CCGUCAUC--CAAcauauc 5'
            |||    |||:||:|   ||      
Target 5' -tGAACCGTGGCGGTGGCCCTTcccggc 3'
 		1 - 27 60.00 -9.16
2 
miRNA  3' uuGGUG-UGUUGGAUGAUGGAGu 5'
            |||| |:|| :| |||::|| 
Target 5' agCCACAATAAATT-CTATTTCa 3'
 		118 - 139 131.00 -15.64
3 
miRNA  3' uuGGUGUGUUGG-AUGAUGGAGu 5'
            | :| ||:||  :| |:||| 
Target 5' gcCGGCCCAGCCAGGCCATCTCa 3'
 		80 - 102 108.00 -15.90
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-let-7b :: SCYL1    [ Functional MTI ]
Validation Method pSILAC
Conditions HeLa
Original Description (Extracted from the article) ... Provided by pSILAC database (http://psilac.mdc-berlin.de/) ...

- Selbach, M. Schwanhausser, B. Thierfelder, et al., 2008, Nature.
Article - Selbach, M. Schwanhausser, B. Thierfelder, et al.
- Nature, 2008
Animal microRNAs (miRNAs) regulate gene expression by inhibiting translation and/or by inducing degradation of target messenger RNAs. It is unknown how much translational control is exerted by miRNAs on a genome-wide scale. We used a new proteomic approach to measure changes in synthesis of several thousand proteins in response to miRNA transfection or endogenous miRNA knockdown. In parallel, we quantified mRNA levels using microarrays. Here we show that a single miRNA can repress the production of hundreds of proteins, but that this repression is typically relatively mild. A number of known features of the miRNA-binding site such as the seed sequence also govern repression of human protein synthesis, and we report additional target sequence characteristics. We demonstrate that, in addition to downregulating mRNA levels, miRNAs also directly repress translation of hundreds of genes. Finally, our data suggest that a miRNA can, by direct or indirect effects, tune protein synthesis from thousands of genes.
LinkOut: [PMID: 18668040]