Accession ID: MIRT001804 [miRNA, hsa-miR-155 :: MATR3, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-155 LinkOut: [miRBase ]
Description Homo sapiens miR-155 stem-loop
Comment Human mir-155 is predicted based on homology to a cloned miR from mouse (MIR:MI0000177) .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-155-3p
Mature Sequence 43| CUCCUACAUAUUAGCAUUAACA |64
Evidence Experimental
Experiments Cloned
Putative hsa-miR-155-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-155-5p
Mature Sequence 4| UUAAUGCUAAUCGUGAUAGGGGU |26
Evidence Experimental
Experiments Cloned
Putative hsa-miR-155-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol MATR3 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms DKFZp686K0542, DKFZp686K23100, KIAA0723, MGC9105, MPD2
Description matrin 3
Transcript NM_018834   LinkOut: [ RefSeq ]
Other Transcripts NM_199189   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on MATR3 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of MATR3
(miRNA target sites are highlighted)		 
>MATR3|NM_018834|3'UTR
   1 TAAGATGTGCAAGGAGATTTAATGATTTCAAAGAAAATAATGGTTCTTTGTTTTTAATGTTAACCTTTTTTAAATACAAT
  81 ACTGATAGTTAGAAGAAAACTATTGTACTCTTTTGTTTTAGTGGAGAAATAATAGATGTCTGTTCATGTGTTAAGTGTTA
 161 TAGCAAAAAAAATACACATATGGTTAAGTTAATGAATAGTTTTTGTTTTATCAGAATGGCAACAGACAGAAGTACTTTGT
 241 AGAGATTGACTTCCTAAGCTACTTAAGACAACTTGCACCACTAAGAAAAAAATGTAGAACCATTTGGAAAAATGAAATTT
 321 AGTAGTTCCAAGTTTCAAAGAAATGTCAACATTTTATTCCATTCAATAAAGAACAAAACCAATAGTGTTTTTATTACTTT
 401 CATCTGAAACATTCCATGTTTTAATCTGAGCCTTGCAGACTTTCATTTGGAGTTTGAACCCGTTTTGGTTGCATTTCATT
 481 TTTGGAGAACTTAATTAACGTGAGATTGGCAATTGAAATGCAGGTGCAGTTTTCTGTTAATGTCATGCTGTTGTTTAGGT
 561 AATAAGAAATATTAAGTAATTGGCTTTAGATTTTGTAATTTTTTTCCCTGAGTTCCTGCTAGATTTCGTATTCTAGTAGT
 641 CAATGTATTTTCAGTGAAATGCAAAAATATTCCCGTTATCTTTGACCAGTATTAATTTTTGAGATCTTACTGCTTGTCAC
 721 TTGAATCCCGTGATTGTCATACATCTCTGGTATAAGCAACATTTGATTTTTGAAGTGTGTAGACCATCTCTTCATATTTT
 801 CAAGATGTAATTTTACATTTCTGCATTTTTAAAACAGTTTGGCCATAATCCTAGATGCACGCTTCTAATTCATGTACCTG
 881 CACATGTGACCTTTGTGAACAGAAATTTGCATGTATAATTTGTGTTTACTTGTAACTTTCTGGTTATATACTGCTTATAT
 961 CTGTGGATTCAAGTTACTGAAGTGAATACCAATAAAAAGAAAACCCTAGGCCATGTTAATTGGTTATACATGTTTGGAAT
1041 GTTAACCAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' acAAUUACGA---UUAUACAUCcuc 5'
            ||:|| :|   |:|:|||||   
Target 5' atTTGATTTTTGAAGTGTGTAGacc 3'
 		761 - 785 124.00 -7.72
2 
miRNA  3' acaAUUACGAUUAUACAUCCuc 5'
             | |||||: |:| ||||  
Target 5' atgTCATGCTGTTGTTTAGGta 3'
 		540 - 561 119.00 -11.70
3 
miRNA  3' acaauuacgAUUAUACAUC-CUc 5'
                   || | ||||| || 
Target 5' agacagaagTACTTTGTAGAGAt 3'
 		224 - 246 117.00 -5.40
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-155 :: MATR3    [ Functional MTI ]
Validation Method Luciferase reporter assay
Conditions BJAB, PEL, BL
Location of target site 3'UTR
Article - Skalsky, R. L. Samols, M. A. Plaisance, K. et al.
- J Virol, 2007
MicroRNAs (miRNAs) are small noncoding RNAs that posttranscriptionally regulate gene expression by binding to 3'-untranslated regions (3'UTRs) of target mRNAs. Kaposi's sarcoma-associated herpesvirus (KSHV), a virus linked to malignancies including primary effusion lymphoma (PEL), encodes 12 miRNA genes, but only a few regulatory targets are known. We found that KSHV-miR-K12-11 shares 100% seed sequence homology with hsa-miR-155, an miRNA frequently found to be up-regulated in lymphomas and critically important for B-cell development. Based on this seed sequence homology, we hypothesized that both miRNAs regulate a common set of target genes and, as a result, could have similar biological activities. Examination of five PEL lines showed that PELs do not express miR-155 but do express high levels of miR-K12-11. Bioinformatic tools predicted the transcriptional repressor BACH-1 to be targeted by both miRNAs, and ectopic expression of either miR-155 or miR-K12-11 inhibited a BACH-1 3'UTR-containing reporter. Furthermore, BACH-1 protein levels are low in cells expressing either miRNA. Gene expression profiling of miRNA-expressing stable cell lines revealed 66 genes that were commonly down-regulated. For select genes, miRNA targeting was confirmed by reporter assays. Thus, based on our in silico predictions, reporter assays, and expression profiling data, miR-K12-11 and miR-155 regulate a common set of cellular targets. Given the role of miR-155 during B-cell maturation, we speculate that miR-K12-11 may contribute to the distinct developmental phenotype of PEL cells, which are blocked in a late stage of B-cell development. Together, these findings indicate that KSHV miR-K12-11 is an ortholog of miR-155.
LinkOut: [PMID: 17881434]