Accession ID: MIRT001992 [miRNA, hsa-miR-182-5p :: ADCY6, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-182LinkOut: [miRBase ]
Description Homo sapiens miR-182 stem-loop
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-182-5p
Mature Sequence 23| UUUGGCAAUGGUAGAACUCACACU |46
Evidence Experimental
Experiments Cloned
Putative hsa-miR-182-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol ADCY6 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms AC6, DKFZp779F075, KIAA0422
Description adenylate cyclase 6
Transcript NM_0152    LinkOut: [ RefSeq ]
Other Transcripts NM_0209   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on ADCY6 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of ADCY6
(miRNA target sites are highlighted)
>ADCY6|NM_0152|3'UTR
   1 TAACAGGGCCCAGCCACAAATTCAGCTGAAGGGACCAAGGTGGGCATTGAGTGGACTCTGTGCTCACTGGGTGGAGCTGT
  81 GGCAGGGGGCACTGAGCCTCCAGACCCTGCTAACCACAAAAGGGAACATCCCAGCAGGCTGTGCTTGGATCATGCTCGTC
 161 TGCCCTCAAGCTGGAAAACAAGGGGCTACCTACCGAGAGGATTATGCAAGTGACTTTCTTTCTTACTTGGGGTAGGGCTG
 241 TTCCCTCTCCAATCTTCCAGCCTTTGGGAGCAGGGGAGGGGTCAGTAGCAGAAGCAGAGGGAGGCCTCTTGCCTGAGGGA
 321 TTAAAATGGCAGCTTGCCATGCCTACCCTTCCCTGTCTGTCTGGGCAGCAGGTTCAGGGCTGAGCCCTTCTTTTCCCTCT
 401 TTTTTCCTGGGAATATTTTGTACAATATTTTGTACAAAGACAGGCATGAGGAGTGCCTATTCCATGCTTGCCTTTGCAAT
 481 ACCTGCATCCCCAGCACTGGTCCTGGGCACTTCCCCACCCCAGCCAGGTGTCCCTCCTATGCACAGAGCAGAGGAGGGAG
 561 AAGCTCTGGGGAGCCAGCTTTGGCCATATTTCAGGAGAATGTTTCCATGTGCCAAATCTTAGTCCCATGATCTGTCCCCA
 641 AAGGGGAACAAAGGGACCTCTGACAGCTTAGATTTAGCCCCAGTTCCTGCACGCTCCAGGGAACGGGGTGTCTGGCCTCA
 721 CTGGTACTGTGAAAAATGCTCAGAGAGCAAGCCTGTGTGTGGGGATGTCAGGTCAGGAGCTGGAAGTTCACCTGCAGGTG
 801 CCAAAGAGCAGGCCGGCCAGGGCTGGGGCAGTGCCAGACTCTGATCTGAGGACCCCGTCGGGGTCCAGATCAGGTCACTC
 881 TGCCCCAGTGCTCTCTTGCTGTCTGCTGACAAGGGGGCATGGAGCATCTCTTCCTCTTCTGTTGCCAAATAGAAAAGGGT
 961 CAGGGCATGGAGAAAGGTGACCCTGATCCCAAACCTGCCCTCCCAAGTCTCTGGTGTTGGGGAGGGCCCGTGTGTTTGTG
1041 TAACTGTGTGTGCATGTTGGTCTTTGTGTGCATATCTGTTTTCCAGGTCTATGTGAGTCCTTGTGCTCCTGCTCCTCAGC
1121 TCTCCACCCCAGGTTGCCTCTCTCCTGTGGGCCTCTGTCTTCTGGGAATAAAGCAGGGTTTCCTACTTCAGGGGATGTAG
1201 AGAGATGCCCAGGTTGCACAGGAGTGGGATGGGGTGTGGTAGCAAAAGGAGGGAGAGGAGTCCTTTTTGTGCCAAATCCC
1281 TAAGTGCCGTTCGGGGGCCATGTGTGCAGCATGACTCTCCCTGTCTGTGGCAGGGACCCAAGCGCTTGCTTAAGCCCCAG
1361 TGCTCCATGCCAGCACTTGAACTGTCTGGGGTTTGATGGACAGAGGCTGAGGAATTTCCTGGCTTCCCCAGATAGTGTCC
1441 TGGGACATGGTATGCTTTGGGGCTGGGGTAGCATGGAATCCCTCTGAGGACCTGGATACTGGTACTACGGGGTGGGGAAG
1521 AGGAACCTTAAACTTGGCTTTCCCCAGCCTTCAGCCTGAGTCTAGCATGTTTCTAGCTCCCCAGTCCCTTGTGAAGCCTT
1601 GAGGGCTGGCAGAAGGGTTAGGAGGTTGAACTCTAGATTCCCTTCCTGTCTTTGCCTTCCTTTTACCCCTTTCCCTGCAA
1681 CCTCCTTGACTCTGGCCTGAATTTGTTGGTGCCTCAGTTTCTCTGTCTGTACCTATTTAAGCCAAAGGCACTAGCCTGAA
1761 TTTTGCTTGAAGATCACTTTGTCTTGGAAATGACTAGAGAGGCAGAGGAGAAGGGTTTCCAGAGTTGCTAGGTTTGGGAG
1841 TGGAAGGGGCAGGCAGTGCACTTGCCCCTCCTCATGCCCCTTCTGACACCAGCTCCCTGTGGAGGCCTGGTTTCTGGGTA
1921 ATGCCTCCCTTGGGCATCTTCATGCATCAACCAAATGGGCCATCAGGTACTTCATTAGTCATGGCAGAAGGAGGGGAAAA
2001 GACTTGTTTTCCAGACAGAAAATCTACTCCCCTGTCCCCAGCCATATCCCTGGATAGGAAGGGATAGGAAGAGACTACTT
2081 GGTGCCATGGGGTAGGGGTGAGGGTATAAGTAGATCAGAGTGGGAAGACCTCAGCCTTGGGTGGCTTGTCTCTGCTTCTT
2161 GCCAGGTGGGAGGGCCTGTCCACACCCTGGATCCCCGTACCACAGTGCCAGCCATGCCCTTCCCCTGGGCTACCATTGTC
2241 CCTTTCCTCACCCAGTTGGTAGAGGAGTCAGGAGGTGGGAGGCCGTGGGCTTTGGTTTTATAATGTAACCACTGTGGGGG
2321 TGGGGGAGGATGGTGAACCATGTATTTCAGTGAAATATTTAATATATTTAAATATCAATAAAATCAAACTCTTTGTAAAA
2401 TTAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' ucacacucaAGAUGGUAACGGUUu 5'
                   ||| |::||||||| 
Target 5' atctcttccTCTTCTGTTGCCAAa 3'
926 - 949 159.00 -13.10
2
miRNA  3' ucaCACU---CA-AGAUG-GUAACGGUUu 5'
             |||:   || |||:| : ||||||: 
Target 5' tggGTGGCTTGTCTCTGCTTCTTGCCAGg 3'
2138 - 2166 141.00 -17.60
3
miRNA  3' ucaCACUCAAGAUGGUA-ACGGUUu 5'
             | || | :| |||| |||||| 
Target 5' cagGAGAATGTTTCCATGTGCCAAa 3'
592 - 616 137.00 -14.50
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-182-5p :: ADCY6    [ Functional MTI ]
Validation Method Luciferase reporter assay ,
Conditions HEK293
Location of target site 3'UTR
Tools used in this research DIANA-microT , miRanda , TargetScan , PicTar
Original Description (Extracted from the article) ... miR-96 or miR-182 down-regulated luciferase activity by 75% ( 2%) and 61% ( 7%), respectively (Fig. 7C). Co-transfection with miR-96 plus miR-182 had the same effect as miR-96 alone (74 4% inhibition). These results strongly support the prediction of Adcy6 as a direct target of miR-96 and miR-182. ...

- Xu, S. Witmer, P. D. Lumayag, S. Kovacs, B. et al., 2007, J Biol Chem.

Article - Xu, S. Witmer, P. D. Lumayag, S. Kovacs, B. et al.
- J Biol Chem, 2007
Although microRNAs (miRNAs) provide a newly recognized level of regulation of gene expression, the miRNA transcriptome of the retina and the contributions of miRNAs to retinal development and function are largely unknown. To begin to understand the functions of miRNAs in retina, we compared miRNA expression profiles in adult mouse retina, brain, and heart by microarray analysis. Our results show that at least 78 miRNAs are expressed in adult mouse retina, 21 of which are potentially retina-specific. Among these, we identified a polycistronic, sensory organ-specific paralogous miRNA cluster that includes miR-96, miR-182, and miR-183 on mouse chromosome 6qA3 with conservation of synteny to human chromosome 7q32.2. In situ hybridization showed that members of this cluster are expressed in photoreceptors, retinal bipolar and amacrine cells. Consistent with their genomic organization, these miRNAs have a similar expression pattern during development with abundance increasing postnatally and peaking in adult retina. Target prediction and in vitro functional studies showed that MITF, a transcription factor required for the establishment and maintenance of retinal pigmented epithelium, is a direct target of miR-96 and miR-182. Additionally, to identify miRNAs potentially involved in circadian rhythm regulation of the retina, we performed miRNA expression profiling with retinal RNA harvested at noon (Zeitgeber time 5) and midnight (Zeitgeber time 17) and identified a subgroup of 12 miRNAs, including members of the miR-183/96/182 cluster with diurnal variation in expression pattern. Our results suggest that miR-96 and miR-182 are involved in circadian rhythm regulation, perhaps by modulating the expression of adenylyl cyclase VI (ADCY6).
LinkOut: [PMID: 17597072]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-182-5p :: ADCY6    [ Functional MTI ]
Validation Method Luciferase reporter assay , qRT-PCR
Conditions HeLa
Location of target site 3'UTR
Tools used in this research miRanda , PicTar , TargetScan
Original Description (Extracted from the article) ... a signi´Čücant reduction in luciferase activity of plasmids with 3' UTR of ADCY6, CLOCK and DSIP genes was shown when transfecting cells with the mutated form of pre-miR-182 compared with cells that did not express miR-182.// ...

- Saus, E. Soria, V. Escaramis, G. Vivarelli, et al., 2010, Hum Mol Genet.

Article - Saus, E. Soria, V. Escaramis, G. Vivarelli, et al.
- Hum Mol Genet, 2010
Previous studies in mice have reported five different microRNAs (miRNAs; miR-219-1/132/183/96/182) to be modulators of the endogenous circadian clock and have presented experimental evidence for some of the genes involved in the molecular clock machinery as target sites. Moreover, disruption of circadian rhythms has long been implicated in the pathophysiology of major depression (MD). We investigated these miRNAs and some of their target sites at the sequence and functional levels as possible predisposing factors for susceptibility to MD and related chronobiological subphenotypes. Mutational screening was performed in a sample of 359 MD patients and 341 control individuals. We found a significant association between the T allele of the rs76481776 polymorphism in the pre-miR-182 and late insomnia in MD patients. Previous studies have reported an association between insomnia and CLOCK gene, a predicted miR-182 target site. A significant overexpression of miR-182 was detected by quantitative real-time polymerase chain reaction in cells transfected with the mutated form of the pre-miR-182 when compared with wild-type form. Moreover, a significant reduction in luciferase activity of plasmids with 3' UTR of ADCY6, CLOCK and DSIP genes was shown when transfecting cells with the mutated form of pre-miR-182 compared with cells that did not express miR-182. These data indicate that abnormal processing of pre-miR-182 in patients carrying the T allele of the rs76481776 polymorphism may contribute to the dysregulation of circadian rhythms in MD patients with insomnia, which could influence expression levels of the mature form of miR-182 and might increase downregulation in some of its target genes.
LinkOut: [PMID: 20656788]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
68 hsa-miR-182-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000576 CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) 4 1
MIRT000671 FOXO3 forkhead box O3 3 2
MIRT001086 FOXO1 forkhead box O1 4 1
MIRT001222 RARG retinoic acid receptor, gamma 3 1
MIRT001990 MITF microphthalmia-associated transcription factor 4 4
MIRT001992 ADCY6 adenylate cyclase 6 3 2
MIRT003145 Mitf microphthalmia-associated transcription factor 3 1
MIRT003601 EP300 E1A binding protein p300 2 1
MIRT005358 CLOCK clock homolog (mouse) 3 1
MIRT005359 TSC22D3 TSC22 domain family, member 3 3 1
MIRT006273 CREB1 cAMP responsive element binding protein 1 1 1
MIRT006300 MTSS1 metastasis suppressor 1 1 3
MIRT006823 FGF9 fibroblast growth factor 9 (glia-activating factor) 2 1
MIRT006824 NTM neurotrimin 2 1
MIRT006978 CYLD cylindromatosis (turban tumor syndrome) 3 1
MIRT007063 BCL2 B-cell CLL/lymphoma 2 3 1
MIRT007064 CCND2 cyclin D2 3 1
MIRT007155 PDCD4 programmed cell death 4 (neoplastic transformation inhibitor) 3 1
MIRT007199 PFN1 profilin 1 1 1
MIRT007226 SNAI2 snail homolog 2 (Drosophila) 1 1
MIRT007293 RECK reversion-inducing-cysteine-rich protein with kazal motifs 3 3
MIRT007306 SMAD4 SMAD family member 4 1 1
MIRT007330 FOXF2 forkhead box F2 1 1
MIRT025033 IGF1R insulin-like growth factor 1 receptor 1 1
MIRT047175 CISD2 CDGSH iron sulfur domain 2 1 1
MIRT047176 BRIP1 BRCA1 interacting protein C-terminal helicase 1 1 1
MIRT047177 RBM12 RNA binding motif protein 12 1 1
MIRT047178 CBX6 chromobox homolog 6 1 1
MIRT047179 FMNL3 formin-like 3 1 1
MIRT047180 RPL5 ribosomal protein L5 1 1
MIRT047181 RCC2 regulator of chromosome condensation 2 1 1
MIRT047182 GNL1 guanine nucleotide binding protein-like 1 1 1
MIRT047183 SNRPA1 small nuclear ribonucleoprotein polypeptide A' 'protein-coding 1 1
MIRT047184 C1orf174 chromosome 1 open reading frame 174 1 1
MIRT047185 RLF rearranged L-myc fusion 1 1
MIRT047186 CASC3 cancer susceptibility candidate 3 1 1
MIRT047187 FLOT1 flotillin 1 1 1
MIRT047188 BAG1 BCL2-associated athanogene 1 1
MIRT047189 RBM15B RNA binding motif protein 15B 1 1
MIRT047190 LSM14A LSM14A, SCD6 homolog A (S. cerevisiae) 1 1
MIRT047191 RPS4X ribosomal protein S4, X-linked 1 1
MIRT047192 UBA1 ubiquitin-like modifier activating enzyme 1 1 1
MIRT047193 RPS3 ribosomal protein S3 1 1
MIRT047194 APITD1 apoptosis-inducing, TAF9-like domain 1 1 1
MIRT047195 TMEM184C transmembrane protein 184C 1 1
MIRT047196 ETV3 ets variant 3 1 1
MIRT047197 SEC61A2 Sec61 alpha 2 subunit (S. cerevisiae) 1 1
MIRT047198 UBP1 upstream binding protein 1 (LBP-1a) 1 1
MIRT047199 USP16 ubiquitin specific peptidase 16 1 1
MIRT047200 ACVR1B activin A receptor, type IB 1 1
MIRT047201 STAG2 stromal antigen 2 1 1
MIRT047202 RAB3IP RAB3A interacting protein (rabin3) 1 1
MIRT047203 ATP5O ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit 1 1
MIRT047204 SLC25A14 solute carrier family 25 (mitochondrial carrier, brain), member 14 1 1
MIRT047205 TTC28 tetratricopeptide repeat domain 28 1 1
MIRT047206 NUFIP2 nuclear fragile X mental retardation protein interacting protein 2 1 1
MIRT047207 TNKS1BP1 tankyrase 1 binding protein 1, 182kDa 1 1
MIRT047208 ACTN4 actinin, alpha 4 1 1
MIRT047209 TUBA1B tubulin, alpha 1b 1 1
MIRT047210 EVI5 ecotropic viral integration site 5 1 1
MIRT047211 VPS51 chromosome 11 open reading frame 2 1 1
MIRT047212 ANO6 anoctamin 6 1 1
MIRT047213 PCOLCE2 procollagen C-endopeptidase enhancer 2 1 1
MIRT047214 LBR lamin B receptor 1 1
MIRT047215 PIK3C2A phosphoinositide-3-kinase, class 2, alpha polypeptide 1 1
MIRT047216 ERO1L ERO1-like (S. cerevisiae) 1 1
MIRT047217 PRKAA2 protein kinase, AMP-activated, alpha 2 catalytic subunit 1 1
MIRT047218 ATP13A3 ATPase type 13A3 1 1