Accession ID: MIRT002579 [miRNA, hsa-miR-124 ::
MYH9, target gene]
| miRNA name | hsa-miR-124 |
|---|
 |
| Gene Symbol | MYH9 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] |
|---|---|
| Synonyms | DFNA17, EPSTS, FTNS, MGC104539, MHA, NMHC-II-A, NMMHCA |
| Description | myosin, heavy chain 9, non-muscle |
| Transcript | NM_002473 LinkOut: [ RefSeq ] |
| Expression | LinkOut: [ BioGPS ] |
| KEGG Pathway |
hsa04530 Tight junction - Homo sapiens (human) hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human) hsa05416 Viral myocarditis - Homo sapiens (human) |
| Putative miRNA Targets on MYH9 | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] |
| 3'UTR of MYH9 (miRNA target sites are highlighted) |
>MYH9|NM_002473|3'UTR 1 CCTGCCGAATAAGCCTCTTCTCCTGCAGCCTGAGATGGATGGACAGACAGACACCACAGCCTCCCCTTCCCAGACCCCGC 81 AGCACGCCTCTCCCCACCTTCTTGGGACTGCTGTGAACATGCCTCCTCCTGCCCTCCGCCCCGTCCCCCCATCCCGTTTC 161 CCTCCAGGTGTTGTTGAGGGCATTTGGCTTCCTCTGCTGCATCCCCTTCCAGCTCCCTCCCCTGCTCAGAATCTGATACC 241 AAAGAGACAGGGCCCGGGCCCAGGCAGAGAGCGACCAGCAGGCTCCTCAGCCCTCTCTTGCCAAAAAGCACAAGATGTTG 321 AGGCGAGCAGGGCAGGCCCCCGGGGAGGGGCCAGAGTTTTCTATGAATCTATTTTTCTTCAGACTGAGGCCTTTTGGTAG 401 TCGGAGCCCCCGCAGTCGTCAGCCTCCCTGACGTCTGCCACCAGCGCCCCCACTCCTCCTCCTTTCTTTGCTGTTTGCAA 481 TCACACGTGGTGACCTCACACACCTCTGCCCCTTGGGCCTCCCACTCCCATGGCTCTGGGCGGTCCAGAAGGAGCAGGCC 561 CTGGGCCTCCACCTCTGTGCAGGGCACAGAAGGCTGGGGTGGGGGGAGGAGTGGATTCCTCCCCACCCTGTCCCAGGCAG 641 CGCCACTGTCCGCTGTCTCCCTCCTGATTCTAAAATGTCTCAAGTGCAATGCCCCCTCCCCTCCTTTACCGAGGACAGCC 721 TGCCTCTGCCACAGCAAGGCTGTCGGGGTCAAGCTGGAAAGGCCAGCAGCCTTCCAGTGGCTTCTCCCAACACTCTTGGG 801 GACCAAATATATTTAATGGTTAAGGGACTTGTCCCAAGTCTGACAGCCAGAGCGTTAGAGGGGCCAGCGGCCCTCCCAGG 881 CGATCTTGTGTCTACTCTAGGACTGGGCCCGAGGGTGGTTTACCTGCACCGTTGACTCAGTATAGTTTAAAAATCTGCCA 961 CCTGCACAGGTATTTTTGAAAGCAAAATAAGGTTTTCTTTTTTCCCCTTTCTTGTAATAAATGATAAAATTCCGAGTCTT 1041 TCTCACTGCCTTTGTTTAGAAGAGAGTAGCTCGTCCTCACTGGTCTACACTGGTTGCCGAATTTACTTGTATTCCTAACT 1121 GTTTTGTATATGCTGCATTGAGACTTACGGCAAGAAGGCATTTTTTTTTTTTAAAGGAAACAAACTCTCAAATCATGAAG 1201 TGATATAAAAGCTGCATATGCCTACAAAGCTCTGAATTCAGGTCCCAGTTGCTGTCACAAAGGAGTGAGTGAAACTCCCA 1281 CCCTACCCCCTTTTTTATATAATAAAAGTGCCTTAGCATGTGTTGCAGCTGTCACCACTACAGTAAGCTGGTTTACAGAT 1361 GTTTTCCACTGAGCATCACAATAAAGAGAACCATGTGCTACGA Target sites Provided by authors Predicted by miRanda |
| miRNA:Target | hsa-miR-124 :: MYH9 [ Functional MTI ] | ||||||
|---|---|---|---|---|---|---|---|
| Validation Method | Microarray | ||||||
| Conditions | HeLa | ||||||
| Location of target site | 3'UTR | ||||||
| Original Description (Extracted from the article) | ... Filtering the expression profiles for genes characterized by the LocusLink database12 that were significantly downregulated (P < 0.001) at both 12 and 24 h, gave sets of 96 and 174 annotated genes downregulated by miR-1 and miR-124, respectively (Supplementary Tables 1 and 2).//{These MTI shown in Supplementary Table 1,2,4} ... - Lim, L. P. Lau, N. C. Garrett-Engele, P. et al., 2005, Nature. |
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| miRNA-target interactions (Provided by authors) |
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| Article |
- Lim, L. P.
Lau, N. C.
Garrett-Engele, P. et al. - Nature, 2005
MicroRNAs (miRNAs) are a class of noncoding RNAs that post-transcriptionally regulate gene expression in plants and animals. To investigate the influence of miRNAs on transcript levels, we transfected miRNAs into human cells and used microarrays to examine changes in the messenger RNA profile. Here we show that delivering miR-124 causes the expression profile to shift towards that of brain, the organ in which miR-124 is preferentially expressed, whereas delivering miR-1 shifts the profile towards that of muscle, where miR-1 is preferentially expressed. In each case, about 100 messages were downregulated after 12 h. The 3' untranslated regions of these messages had a significant propensity to pair to the 5' region of the miRNA, as expected if many of these messages are the direct targets of the miRNAs. Our results suggest that metazoan miRNAs can reduce the levels of many of their target transcripts, not just the amount of protein deriving from these transcripts. Moreover, miR-1 and miR-124, and presumably other tissue-specific miRNAs, seem to downregulate a far greater number of targets than previously appreciated, thereby helping to define tissue-specific gene expression in humans.
LinkOut: [PMID: 15685193]
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