Accession ID: MIRT003183 [miRNA, hsa-miR-210 ::
APC, target gene]
| pre-miRNA ID | hsa-mir-210 LinkOut: [miRBase ] |
|---|---|
| Synonyms | MIRN210, mir-210, MIR210 |
| Description | Homo sapiens miR-210 stem-loop |
| Comment | This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences . |
| 2nd Structure of pre-miRNA |  |
| Mature miRNA | hsa-miR-210 |
|---|---|
| Mature Sequence | 66| CUGUGCGUGUGACAGCGGCUGA |87 |
| Evidence | Experimental |
| Experiments | Cloned |
| Putative hsa-miR-210 Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
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| Gene Symbol | APC LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Synonyms | BTPS2, DP2, DP2.5, DP3, GS | ||||||||||||||||||||
| Description | adenomatous polyposis coli | ||||||||||||||||||||
| Transcript | NM_000038 LinkOut: [ RefSeq ] | ||||||||||||||||||||
| Other Transcripts | NM_001127510, NM_001127511 | ||||||||||||||||||||
| Expression | LinkOut: [ BioGPS ] | ||||||||||||||||||||
| KEGG Pathway |
hsa04310 Wnt signaling pathway - Homo sapiens (human) hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human) hsa05200 Pathways in cancer - Homo sapiens (human) hsa05210 Colorectal cancer - Homo sapiens (human) hsa05213 Endometrial cancer - Homo sapiens (human) hsa05217 Basal cell carcinoma - Homo sapiens (human) |
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| Putative miRNA Targets on APC | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] | ||||||||||||||||||||
| 3'UTR of APC (miRNA target sites are highlighted) |
>APC|NM_000038|3'UTR 1 TAAAAGAGAGGAAGAATGAAACTAAGAAAATTCTATGTTAATTACAACTGCTATATAGACATTTTGTTTCAAATGAAACT 81 TTAAAAGACTGAAAAATTTTGTAAATAGGTTTGATTCTTGTTAGAGGGTTTTTGTTCTGGAAGCCATATTTGATAGTATA 161 CTTTGTCTTCACTGGTCTTATTTTGGGAGGCACTCTTGATGGTTAGGAAAAAAATAGTAAAGCCAAGTATGTTTGTACAG 241 TATGTTTTACATGTATTTAAAGTAGCATCCCATCCCAACTTCCTTTAATTATTGCTTGTCTTAAAATAATGAACACTACA 321 GATAGAAAATATGATATATTGCTGTTATCAATCATTTCTAGATTATAAACTGACTAAACTTACATCAGGGAAAAATTGGT 401 ATTTATGCAAAAAAAAATGTTTTTGTCCTTGTGAGTCCATCTAACATCATAATTAATCATGTGGCTGTGAAATTCACAGT 481 AATATGGTTCCCGATGAACAAGTTTACCCAGCCTGCTTTGCTTTACTGCATGAATGAAACTGATGGTTCAATTTCAGAAG 561 TAATGATTAACAGTTATGTGGTCACATGATGTGCATAGAGATAGCTACAGTGTAATAATTTACACTATTTTGTGCTCCAA 641 ACAAAACAAAAATCTGTGTAACTGTAAAACATTGAATGAAACTATTTTACCTGAACTAGATTTTATCTGAAAGTAGGTAG 721 AATTTTTGCTATGCTGTAATTTGTTGTATATTCTGGTATTTGAGGTGAGATGGCTGCTCTTTTATTAATGAGACATGAAT 801 TGTGTCTCAACAGAAACTAAATGAACATTTCAGAATAAATTATTGCTGTATGTAAACTGTTACTGAAATTGGTATTTGTT 881 TGAAGGGTCTTGTTTCACATTTGTATTAATAATTGTTTAAAATGCCTCTTTTAAAAGCTTATATAAATTTTTTTCTTCAG 961 CTTCTATGCATTAAGAGTAAAATTCCTCTTACTGTAATAAAAACAATTGAAGAAGACTGTTGCCACTTAACCATTCCATG 1041 CGTTGGCACTTATCTATTCCTGAAATTTCTTTTATGTGATTAGCTCATCTTGATTTTTAATATTTTTCCACTTAAACTTT 1121 TTTTTCTTACTCCACTGGAGCTCAGTAAAAGTAAATTCATGTAATAGCAATGCAAGCAGCCTAGCACAGACTAAGCATTG 1201 AGCATAATAGGCCCACATAATTTCCTCTTTCTTAATATTATAGAATTCTGTACTTGAAATTGATTCTTAGACATTGCAGT 1281 CTCTTCGAGGCTTTACAGTGTAAACTGTCTTGCCCCTTCATCTTCTTGTTGCAACTGGGTCTGACATGAACACTTTTTAT 1361 CACCCTGTATGTTAGGGCAAGATCTCAGCAGTGAAGTATAATCAGCACTTTGCCATGCTCAGAAAATTCAAATCACATGG 1441 AACTTTAGAGGTAGATTTAATACGATTAAGATATTCAGAAGTATATTTTAGAATCCCTGCCTGTTAAGGAAACTTTATTT 1521 GTGGTAGGTACAGTTCTGGGGTACATGTTAAGTGTCCCCTTATACAGTGGAGGGAAGTCTTCCTTCCTGAAGGAAAATAA 1601 ACTGACACTTATTAACTAAGATAATTTACTTAATATATCTTCCCTGATTTGTTTTAAAAGATCAGAGGGTGACTGATGAT 1681 ACATGCATACATATTTGTTGAATAAATGAAAATTTATTTTTAGTGATAAGATTCATACACTCTGTATTTGGGGAGGGAAA 1761 ACCTTTTTAAGCATGGTGGGGCACTCAGATAGGAGTGAATACACCTACCTGGTGCCTTGAAAATCACATCAAGTAGTTAA 1841 TTATCTACCCCTTACCTGTGTTTATAACTTCCAGGTAATGAGAATGATTTTTTTTAAAGCTAAAATGCCAGTAAATAAAA 1921 GTGCTATGACTTGAGCTAAGATATTTGACTCCAATGCCTGTACTGTGTCTACTGCACCACTTTGTAAACACTTCAATTTA 2001 CTATCTTTGAAATGATTGACCTTTAAATTTTTGCCAAATGTTATCTGAAATTGTCTATGAATACCATCTACTTCTGTTGT 2081 TTTCCCAGGCTTCCATAAACAATGGAGATACATGCA Target sites Provided by authors Predicted by miRanda |
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| miRNA-target interactions (Predicted by miRanda) |
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| miRNA:Target | hsa-miR-210 :: APC [ Functional MTI ] | ||||||
|---|---|---|---|---|---|---|---|
| Validation Method | immunoprecipitaion , Microarray , qRT-PCR | ||||||
| Conditions | HEK293, HUVEC, MCF7 | ||||||
| Location of target site | CDS | ||||||
| Tools used in this research | DIANA-microT, EIMMO, miRanda, miRBase Target Database, PicTar, TargetScan | ||||||
| miRNA-target interactions (Provided by authors) |
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| Article |
- Fasanaro, P.
Greco, S.
Lorenzi, M. et al. - J Biol Chem, 2009
miR-210 is a key player of cell response to hypoxia, modulating cell survival, VEGF-driven endothelial cell migration and the ability of endothelial cells to form capillary-like structures. A crucial step in understanding miRNA function is the identification of their targets. However, only few miR-210 targets have been identified to date. Here we describe an integrated strategy for large-scale identification of new miR-210 targets, by combining transcriptomics and proteomics with bioinformatic approaches. To experimentally validate candidate targets, the RNA-Induced Silencing Complex (RISC) loaded with miR-210 was purified by immuno-precipitation along with its mRNA targets. The complex was significantly enriched in mRNAs of 31 candidate targets, such as BDNF, GPD1L, ISCU, NCAM and the non-coding RNA Xist. A sub-set of the newly identified targets was further confirmed by 3'UTR-reporter assays, and hypoxia induced down-modulation of their expression was rescued blocking miR-210, providing support for the approach validity. In the case of 9 targets, such as PTPN1 and P4HB, miR-210-seed-pairing sequences localized in the coding-sequence or in the 5'UTR, in line with recent data extending miRNA targeting beyond the ''classic'' 3'UTR recognition. Finally, Gene Ontology analysis of the targets highlights known miR-210 impact on cell cycle regulation and differentiation, and predicts a new role of this miRNA in RNA processing, DNA binding, development, membrane trafficking and aminoacid catabolism. Given the complexity of miRNA actions, we view such multiprong approach as useful to adequately describe the multiple pathways regulated by miR-210 during physio-pathological processes.
LinkOut: [PMID: 19826008]
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