Accession ID: MIRT003205 [miRNA, kshv-miR-K12-6-5p :: MAF, target gene]
miRNA Infomation
miRNA namekshv-miR-K12-6-5p
Gene Information
Gene Symbol MAF LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms CCA4, c-MAF
Description v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
Transcript NM_0010318    LinkOut: [ RefSeq ]
Other Transcripts NM_0053   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on MAF LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of MAF
(miRNA target sites are highlighted)
>MAF|NM_0010318|3'UTR
   1 GTCTGACACGCGATTCCAGCTAGCCACCCTGATAAGTGCTCCGCGGGGGTCCGGCTCGGGTGTGGGCTTGCTAGTTCTAG
  81 AGCCATGCTCGCCACCACCTCACCACCCCCACCCCCACCGAGTTTGGCCCCCTTGGCCCCCTACACACACACAAACCCGC
 161 ACGCACACACCACACACACACACACACACACACACACACCCCACACCCTGCTCGAGTTTGTGGTGGTGGTGGCTGTTTTA
 241 AACTGGGGAGGGAATGGGTGTCTGGCTCATGGATTGCCAATCTGAAATTCTCCATAACTTGCTAGCTTGTTTTTTTTTTT
 321 TTTTTACACCCCCCCGCCCCACCCCCGGACTTGCACAATGTTCAATGATCTCAGCAGAGTTCTTCATGTGAAACGTTGAT
 401 CACCTTTGAAGCCTGCATCATTCACATATTTTTTCTTCTTCTTCCCCTTCAGTTCATGAACTGGTGTTCATTTTCTGTGT
 481 GTGTGTGTGTTTTATTTTGTTTGGATTTTTTTTTTTAATTTTACTTTTAGAGCTTGCTGTGTTGCCCACCTTTTTTCCAA
 561 CCTCCACCCTCACTCCTTCTCAACCCATCTCTTCCGAGATGAAAGAAAAAAAAAAGCAAAGTTTTTTTTTCTTCTCCTGA
 641 GTTCTTCATGTGAGATTGAGCTTGCAAAGGAAAAAAAAATGTGAAATGTTATAGACTTGCAGCGTGCCGAGTTCCATCGG
 721 GTTTTTTTTTTAGCATTGTTATGCTAAAATAGAGAAAAAAATCCTCATGAACCTTCCACAATCAAGCCTGCATCAACCTT
 801 CTGGGTGTGACTTGTGAGTTTTGGCCTTGTGATGCCAAATCTGAGAGTTTAGTCTGCCATTAAAAAAACTCATTCTCATC
 881 TCATGCATTATTATGCTTGCTACTTTGTCTTAGCAACAATGAACTATAACTGTTTCAAAGACTTTATGGAAAAGAGACAT
 961 TATATTAATAAAAAAAAAAAGCCTGCATGCTGGACATGTATGGTATAATTATTTTTTCCTTTTTTTTTCCTTTTGGCTTG
1041 GAAATGGACGTTCGAAGACTTATAGCATGGCATTCATACTTTTGTTTTATTGCCTCATGACTTTTTTGAGTTTAGAACAA
1121 AACAGTGCAACCGTAGAGCCTTCTTCCCATGAAATTTTGCATCTGCTCCAAAACTGCTTTGAGTTACTCAGAACTTCAAC
1201 CTCCCAATGCACTGAAGGCATTCCTTGTCAAAGATACCAGAATGGGTTACACATTTAACCTGGCAAACATTGAAGAACTC
1281 TTAATGTTTTCTTTTTAATAAGAATGACGCCCCACTTTGGGGACTAAAATTGTGCTATTGCCGAGAAGCAGTCTAAAATT
1361 TATTTTTTAAAAAGAGAAACTGCCCCATTATTTTTGGTTTGTTTTATTTTTATTTTATATTTTTTGGCTTTTGGTCATTG
1441 TCAAATGTGGAATGCTCTGGGTTTCTAGTATATAATTTAATTCTAGTTTTTATAATCTGTTAGCCCAGTTAAAATGTATG
1521 CTACAGATAAAGGAATGTTATAGATAAATTTGAAAGAGTTAGGTCTGTTTAGCTGTAGATTTTTTAAACGATTGATGCAC
1601 TAAATTGTTTACTATTGTGATGTTAAGGGGGGTAGAGTTTGCAAGGGGACTGTTTAAAAAAAGTAGCTTATACAGCATGT
1681 GCTTGCAACTTAAATATAAGTTGGGTATGTGTAGTCTTTGCTATACCACTGACTGTATTGAAAACCAAAGTATTAAGAGG
1761 GGAAACGCCCCTGTTTATATCTGTAGGGGTATTTTACATTCAAAAATGTATGTTTTTTTTTCTTTTCAAAATTAAAGTAT
1841 TTGGGACTGAATTGCACTAAGATATAACCTGCAAGCATATAATACAAAAAAAAATTGCAAAACTGTTTAGAACGCTAATA
1921 AAATTTATGCAGTTATAAAAATGGCATTACTGCACAGTTTTAAGATGATGCAGATTTTTTTACAGTTGTATTGTGGTGCA
2001 GAACTGGATTTTCTGTAACTTAAAAAAAAATCCACAGTTTTAAAGGCAATAATCAGTAAATGTTATTTTCAGGGACTGAC
2081 ATCCTGTCTTTAAAAAGAAATGAAAAGTAAATCTTACCACAATAAATATAAAAAAATCTTGTCAGTTACTTTTCTTTTAC
2161 ATATTTTGCTGTGCAAAATTGTTTTATATCTTGAGTTACTAACTAACCACGCGTGTTGTTCCTATGTGCTTTTCTTTCAT
2241 TTTCAATTCTGGTTATATCAAGAAAAGAATAATCTACAATAATAAACGGCATTTTTTTTTGATTCTGTACTCAGTTTCTT
2321 AGTGTACAGTTTAACTGGGCCCAACAACCTCGTTAAAAGTGTAAAATGCATCCTTTTCTCCAGTGGAAGGATTCCTGGAG
2401 GAATAGGGAGACAGTAATTCAGGGTGAAATTATAGGCTGTTTTTTGAAGTGAGGAGGCTGGCCCCATATACTGATTAGCA
2481 ATATTTAATATAGATGTAAATTATGACCTCATTTTTTTCTCCCCAAAGTTTTCAGTTTTCAAATGAGTTGAGCCATAATT
2561 GCCCTTGGTAGGAAAAACAAAACAAAACAGTGGAACTAGGCTTCCTGAGCATGGCCCTACACTTCTGATCAGGAGCAAAG
2641 CCATCCATAGACAGAGGAGCCGGACAAATATGGCGCATCAGAGGTGGCTTGCGCACATATGCATTGAACGGTAAAGAGAA
2721 ACAGCGCTTGCCTTTTCACTAAAGTTGACTATTTTTCCTTCTTCTCTTACACACCGAGATTTTCTTGTTAGCAAGGCCTG
2801 ACAAGATTTAACATAAACATGACAAATCATAGTTGTTTGTTTTGTTTTGCTTTTCTCTTTAACACTGAAGATCATTTGTC
2881 TTAAATAGGAAAAAGAAAATCCACTCCTTACTTCCATATTTCCAAGTACATATCTGGTTTAAACTATGTTATCAAATCAT
2961 ATTTCACCGTGAATATTCAGTGGAGAACTTCTCTACCTGGATGAGCTAGTAATGATTTCAGATCATGCTATCCCCAGAAA
3041 TAAAAGCAAAAAATAATACCTGTGTGGAATATAGGCTGTGCTTTGATTTACTGGTATTTACCCCAAAATAGGCTGTGTAT
3121 GGGGGCTGACTTAAAGATCCCTTGGAAAGACTCAAAACTACCTTCACTAGTAGGACTCCTAAGCGCTGACCTATTTTTAA
3201 ATGACACAAATTCATGAAACTAATGTTACAAATTCATGCAGTTTGCACTCTTAGTCATCTTCCCCTAGCACACCAATAGA
3281 ATGTTAGACAAAGCCAGCACTGTTTTGAAAATACAGCCAAACACGATGACTTTTGTTTTGTTTTCTGCCGTTCTTAAAAG
3361 AAAAAAAGATAATATTGCAACTCTGACTGAAAGACTTATTTTTAAGAAAACAGGTTGTGTTTGGTGCTGCTAAGTTCTGG
3441 CCAGTTTATCATCTGGCCTTCCTGCCTATTTTTTACAAAACACGAAGACAGTGTGTAACCTCGACATTTTGACCTTCCTT
3521 TATGTGCTAGTTTAGACAGGCTCCTGAATCCACACTTAATTTTGCTTAACAAAAGTCTTAATAGTAAACCTCCCCTCATG
3601 AGCTTGAAGTCAAGTGTTCTTGACTTCAGATATTTCTTTCCTTTTTTTTTTTTTTTCCTCATCACAACTAAGAGATACAC
3681 AAACTCTGAAGAAGCAGAAATGGAGAGAATGCTTTTAACAAAAAAGCATCTGATGAAAGATTTTAGGCAAACATTCTCAA
3761 AATAAGAGTGATATTCTGGATGTAGTTATTGCAGTTATCTCATGACAAATGAGGCCTGGATTGGAAGGAAAATATAGTTG
3841 TGTAGAATTAAGCATTTTGATAGGAATCTACAAGGTAGTTGAATATAATAAGCAGGTTTGGGCCCCCAAACTTTAGAAAA
3921 TCAAATGCAAAGGTGCTGGCAAAAATGAGGTTTGAGTGGCTGGCTGTAAGAGAAGGTTAACTCCTAGTAAAAGGCATTTT
4001 TAGAAATAACAATTACTGAAAACTTTGAAGTATAGTGGGAGTAGCAAACAAATACATGTTTTTTTTTTCTTACAAAGAAC
4081 TCCTAAATCCTGAGTAAGTGCCATTCATTACAATAAGTCTCTAAATTTAAAAAAAAAAAAATCATATGAGGAAATCTAGC
4161 TTTCCCCTTTACGCTGCGTTTGATCTTTGTCTAAATAGTGTTAAAATTCCTTTCATTCCAATTACAGAACTGAGCCCACT
4241 CGCAAGTTGGAGCCATCAGTGGGATACGCCACATTTTGGAAGCCCCAGCATCGTGTACTTACCAGTGTGTTCACAAAATG
4321 AAATTTGTGTGAGAGCTGTACATTAAAAAAAATCATCATTATTATTATTATTTGCAGTCATGGAGAACCACCTACCCCTG
4401 ACTTCTGTTTAGTCTCCTTTTTAAATAAAAATTACTGTGTTAGAGAAGAAGGCTATTAAATGTAGTAGTTAACTATGCCT
4481 CTTGTCTGGGGGTTTCATAGAGACCGGTAGGAAAGCGCACTCCTGCTTTTCGATTTATGGTGTGTGCAAGTAAACAGGTG
4561 CATTGCTTTCAACCTGCCATACTAGTTTTAAAAATTCACTGAAATTACAAAGATACATATATATGCATATATATAATGGA
4641 AAGTTTCCCGGAATGCAACAATTAGCATTTTAAAATCATATATAGGCATGCACATTCTAAATAGTACTTTTTCATGCTTC
4721 ATTGTTTCTCTGGCAGATAATTTTACTAAGAAGAAAAATAGATATTCGACTCCCCTTCCCTAAACAAATCCACGGGCAGA
4801 GGCTCCAGCGGAGCCGAGCCCCCTGGTTTTCTCGTAGGCCCTAGACGGTGTTGCATTTATCAGTGATGTCAAACGTGCTC
4881 ATTTGTCAGACATAGCTGTAAATGAAAACAATGTGTGGCAAAATACAAAGTTA
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target kshv-miR-K12-6-5p :: MAF    [ Functional MTI ]
Validation Method qRT-PCR , Other
Conditions HEK293
Location of target site 3'UTR
Tools used in this research PITA
Original Description (Extracted from the article) ... Site-directed mutagenesis of KSHV miRNA predicted target sites in the MAF 3`UTR abolished silencing (Fig. 3D; Supplemental Fig. 2D), confirming that miR-K12-1, miR-K12-6-5p, and miR-K12-11 regulate MAF by interacting with the 3`UTR ...

- Hansen, A. Henderson, S. Lagos, D. et al., 2010, Genes Dev.

Article - Hansen, A. Henderson, S. Lagos, D. et al.
- Genes Dev, 2010
Kaposi sarcoma herpesvirus (KSHV) induces transcriptional reprogramming of endothelial cells. In particular, KSHV-infected lymphatic endothelial cells (LECs) show an up-regulation of genes associated with blood vessel endothelial cells (BECs). Consequently, KSHV-infected tumor cells in Kaposi sarcoma are poorly differentiated endothelial cells, expressing markers of both LECs and BECs. MicroRNAs (miRNAs) are short noncoding RNA molecules that act post-transcriptionally to negatively regulate gene expression. Here we validate expression of the KSHV-encoded miRNAs in Kaposi sarcoma lesions and demonstrate that these miRNAs contribute to viral-induced reprogramming by silencing the cellular transcription factor MAF (musculoaponeurotic fibrosarcoma oncogene homolog). MAF is expressed in LECs but not in BECs. We identify a novel role for MAF as a transcriptional repressor, preventing expression of BEC-specific genes, thereby maintaining the differentiation status of LECs. These findings demonstrate that viral miRNAs could influence the differentiation status of infected cells, and thereby contribute to KSHV-induced oncogenesis.
LinkOut: [PMID: 20080955]
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
1 kshv-miR-K12-6-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT003205 MAF v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) 2 1