Accession ID: MIRT003226 [miRNA, hsa-miR-424-5p :: MAP2K1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-424LinkOut: [miRBase ]
Synonyms MIR322, MIRN424, hsa-mir-424, miRNA424, MIR424
Description Homo sapiens miR-424 stem-loop
Comment This hairpin precursor expresses a 5' arm product, named miR-424, in human promyelocytic leukemia (HL-60) cells . The level of expression of miR-424 was shown to be up-regulated 48 hours after TPA-induction. The sequence is orthologous to the experimentally verified rat miR-322 locus (MIR:MI0000589), which expresses its mature product from the 3' arm of the hairpin. The human miR-424 hairpin does not appear to contain the miR-322 sequence.
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-424-5p
Mature Sequence 11| CAGCAGCAAUUCAUGUUUUGAA |32
Evidence Experimental
Experiments Cloned
Putative hsa-miR-424-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol MAP2K1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms MAPKK1, MEK1, MKK1, PRKMK1
Description mitogen-activated protein kinase kinase 1
Transcript NM_0027    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on MAP2K1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of MAP2K1
(miRNA target sites are highlighted)
>MAP2K1|NM_0027|3'UTR
   1 TAAGTGTTTGGGAAGCAACAAAGAGCGAGTCCCCTGCCCGGTGGTTTGCCATGTCGCTTTTGGGCCTCCTTCCCATGCCT
  81 GTCTCTGTTCAGATGTGCATTTCACCTGTGACAAAGGATGAAGAACACAGCATGTGCCAAGATTCTACTCTTGTCATTTT
 161 TAATATTACTGTCTTTATTCTTATTACTATTATTGTTCCCCTAAGTGGATTGGCTTTGTGCTTGGGGCTATTTGTGTGTA
 241 TGCTGATGATCAAAACCTGTGCCAGGCTGAATTACAGTGAAATTTTGGTGAATGTGGGTAGTCATTCTTACAATTGCACT
 321 GCTGTTCCTGCTCCATGACTGGCTGTCTGCCTGTATTTTCGGGATTCTTTGACATTTGGTGGTACTTTATTCTTGCTGGG
 401 CATACTTTCTCTCTAGGAGGGAGCCTTGTGAGATCCTTCACAGGCAGTGCATGTGAAGCATGCTTTGCTGCTATGAAAAT
 481 GAGCATCAGAGAGTGTACATCATGTTATTTTATTATTATTATTTGCTTTTCATGTAGAACTCAGCAGTTGACATCCAAAT
 561 CTAGCCAGAGCCCTTCACTGCCATGATAGCTGGGGCTTCACCAGTCTGTCTACTGTGGTGATCTGTAGACTTCTGGTTGT
 641 ATTTCTATATTTATTTTCAGTATACTGTGTGGGATACTTAGTGGTATGTCTCTTTAAGTTTTGATTAATGTTTCTTAAAT
 721 GGAATTATTTTGAATGTCACAAATTGATCAAGATATTAAAATGTCGGATTTATCTTTCCCCATATCCAAGTACCAATGCT
 801 GTTGTAAACAACGTGTATAGTGCCTAAAATTGTATGAAAATCCTTTTAACCATTTTAACCTAGATGTTTAACAAATCTAA
 881 TCTCTTATTCTAATAAATATACTATGAAATAAAAAAAAAAGGATGAAAGCTAAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' aaGUUUUGUACUUAACGACGAc 5'
            ::||:||||  |||||||| 
Target 5' tgTGAAGCATGCTTTGCTGCTa 3'
452 - 473 172.00 -17.60
2
miRNA  3' aaGUUUUGUACUUAACGACGAc 5'
            :| ||: || | |||||:| 
Target 5' ctTACAAT-TGCACTGCTGTTc 3'
307 - 327 139.00 -8.80
3
miRNA  3' aaGUUUUGUACUUAACGACGAc 5'
            | ||::|  || |||||:| 
Target 5' atCCAAGTACCAA-TGCTGTTg 3'
784 - 804 139.00 -10.64
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-424-5p :: MAP2K1    [ Functional MTI ]
Validation Method Luciferase reporter assay , Other
Conditions HTR-8/SVneo cells
Location of target site 3'UTR
Tools used in this research miRBase Target Database , TargetScan
Original Description (Extracted from the article) ... We found that MED1 was silenced 2-fold by miR-205. Likewise, FGFR1 and MAP2K1 were silenced 2-fold by miR-424.//The miR-424 target MAP2K1 plays an essential role in murine placental development. ...

- Mouillet, J. F. Chu, T. Nelson, D. M. et al., 2010, FASEB J.

Article - Mouillet, J. F. Chu, T. Nelson, D. M. et al.
- FASEB J, 2010
Acting through degradation of target mRNA or inhibition of translation, microRNAs (miRNAs) regulate development, differentiation, and cellular response to diverse cues. We analyzed changes in miRNA expression in human placental trophoblasts exposed to hypoxia, which may result from hypoperfusion and placental injury. Using an miRNA microarray screen, confirmed by Northern blot analysis, we defined a set of seven miRNAs (miR-93, miR-205, miR-224, miR-335, miR-424, miR-451, and miR-491) that are differentially regulated in primary trophoblasts exposed to hypoxia. We combined in silico prediction of miRNA targets with gene expression profiling data to identify a series of potential targets for the miRNAs, which were further analyzed using luciferase reporter assays. Among experimentally confirmed targets, we found that the transcriptional coactivator MED1, which plays an important role in placental development, is a target for miR-205. Using gain- and loss-of-function assays, we confirmed that miR-205 interacts with a specific target in the 3;-UTR sequence of MED1 and silences MED1 expression in human trophoblasts exposed to hypoxia, suggesting that miR-205 plays a role in trophoblast injury.-Mouillet, J.-F., Chu, T., Nelson, D. M., Mishima, T., Sadovsky, Y. MiR-205 silences MED1 in hypoxic primary human trophoblasts.
LinkOut: [PMID: 20065103]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-424-5p :: MAP2K1    [ Functional MTI ]
Validation Method Immunohistochemistry , qRT-PCR , Western blot
Conditions HDMEC , CC-2543
Disease senile hemangioma
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... This is the first report implicating miR-424 in the angiogenic process and identifying MEK1 and Cyclin E1 as the true target genes of miR-424 ...

- Nakashima, T. Jinnin, M. Etoh, T. et al., 2010, PLoS One.

Article - Nakashima, T. Jinnin, M. Etoh, T. et al.
- PLoS One, 2010
BACKGROUND: Senile hemangioma, so-called cherry angioma, is known as the most common vascular anomalies specifically seen in the aged skin. The pathogenesis of its abnormal angiogenesis is still unclear. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we found that senile hemangioma consisted of clusters of proliferated small vascular channels in upper dermis, indicating that this tumor is categorized as a vascular tumor. We then investigated the mechanism of endothelial proliferation in senile hemangioma, focusing on microRNA (miRNA). miRNA PCR array analysis revealed the mir-424 level in senile hemangioma was lower than in other vascular anomalies. Protein expression of MEK1 and cyclin E1, the predicted target genes of mir-424, was increased in senile hemangioma compared to normal skin or other anomalies, but their mRNA levels were not. The inhibition of mir-424 in normal human dermal microvascular ECs (HDMECs) using specific inhibitor in vitro resulted in the increase of protein expression of MEK1 or cyclin E1, while mRNA levels were not affected by the inhibitor. Specific inhibitor of mir-424 also induced the cell proliferation of HDMECs significantly, while the cell number was decreased by the transfection of siRNA for MEK1 or cyclin E1. CONCLUSIONS/SIGNIFICANCE: Taken together, decreased mir-424 expression and increased levels of MEK1 or cyclin E1 in senile hemangioma may cause abnormal cell proliferation in the tumor. Senile hemangioma may be the good model for cutaneous angiogenesis. Investigation of senile hemangioma and the regulatory mechanisms of angiogenesis by miRNA in the aged skin may lead to new treatments using miRNA by the transfection into senile hemangioma.
LinkOut: [PMID: 21179471]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
48 hsa-miR-424-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000653 CCNF cyclin F 2 1
MIRT000654 CDC14A CDC14 cell division cycle 14 homolog A (S. cerevisiae) 2 1
MIRT000655 CDC25A cell division cycle 25 homolog A (S. pombe) 2 1
MIRT000656 CHEK1 CHK1 checkpoint homolog (S. pombe) 2 1
MIRT000657 KIF23 kinesin family member 23 2 1
MIRT000658 ATF6 activating transcription factor 6 2 1
MIRT000659 WEE1 WEE1 homolog (S. pombe) 3 2
MIRT000660 ANLN anillin, actin binding protein 2 1
MIRT000696 PLAG1 pleiomorphic adenoma gene 1 4 1
MIRT000936 CCNE1 cyclin E1 4 3
MIRT000937 CCND3 cyclin D3 4 1
MIRT000938 CDK6 cyclin-dependent kinase 6 4 1
MIRT000941 CCND1 cyclin D1 4 2
MIRT003225 ITPR1 inositol 1,4,5-triphosphate receptor, type 1 2 1
MIRT003226 MAP2K1 mitogen-activated protein kinase kinase 1 4 2
MIRT003227 PIAS1 protein inhibitor of activated STAT, 1 2 1
MIRT003228 FGFR1 fibroblast growth factor receptor 1 2 1
MIRT003928 NFIA nuclear factor I/A 3 2
MIRT005886 MYB v-myb myeloblastosis viral oncogene homolog (avian) 3 1
MIRT005887 SIAH1 seven in absentia homolog 1 (Drosophila) 3 1
MIRT005926 HIF1A hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) 5 1
MIRT005927 CUL2 cullin 2 5 1
MIRT005928 SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1 5 1
MIRT042434 CCIN calicin 1 1
MIRT042435 TTC39A tetratricopeptide repeat domain 39A 1 1
MIRT042436 TMF1 TATA element modulatory factor 1 1 1
MIRT042437 HIST1H2BK histone cluster 1, H2bk 1 1
MIRT042438 PNISR splicing factor, arginine/serine-rich 18 1 1
MIRT042439 IARS isoleucyl-tRNA synthetase 1 1
MIRT042440 HIST2H2AA3 histone cluster 2, H2aa3 1 1
MIRT042441 YWHAQ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide 1 1
MIRT042442 SMURF1 SMAD specific E3 ubiquitin protein ligase 1 1 1
MIRT042443 MAGED1 melanoma antigen family D, 1 1 1
MIRT042444 CNIH4 cornichon homolog 4 (Drosophila) 1 1
MIRT042445 ANKHD1 ankyrin repeat and KH domain containing 1 1 1
MIRT042446 EIF4G2 eukaryotic translation initiation factor 4 gamma, 2 1 1
MIRT042447 BAZ2B bromodomain adjacent to zinc finger domain, 2B 1 1
MIRT042448 APBB2 amyloid beta (A4) precursor protein-binding, family B, member 2 1 1
MIRT042449 ANKRD17 ankyrin repeat domain 17 1 1
MIRT042450 RBM4B RNA binding motif protein 4B 1 1
MIRT042451 LAMTOR4 chromosome 7 open reading frame 59 1 1
MIRT042452 TRAP1 TNF receptor-associated protein 1 1 1
MIRT042453 SCOC short coiled-coil protein 1 1
MIRT042454 SACS spastic ataxia of Charlevoix-Saguenay (sacsin) 1 1
MIRT042455 IFIH1 interferon induced with helicase C domain 1 1 1
MIRT042456 TBPL1 TBP-like 1 1 1
MIRT042457 SMARCA5 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 1 1
MIRT042458 GTF2A1 general transcription factor IIA, 1, 19/37kDa 1 1