Accession ID: MIRT003271 [miRNA, hsa-miR-204 ::
TGFBR1, target gene]
| pre-miRNA ID | hsa-mir-204 LinkOut: [miRBase ] |
|---|---|
| Description | Homo sapiens miR-204 stem-loop |
| Comment | This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences . |
| 2nd Structure of pre-miRNA |  |
| Mature miRNA | hsa-miR-204-3p |
|---|---|
| Mature Sequence | 72| GCUGGGAAGGCAAAGGGACGU |92 |
| Evidence | Not_experimental |
| Experiments | |
| Putative hsa-miR-204-3p Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
| Mature miRNA | hsa-miR-204-5p |
| Mature Sequence | 33| UUCCCUUUGUCAUCCUAUGCCU |54 |
| Evidence | Experimental |
| Experiments | Cloned |
| Putative hsa-miR-204-5p Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
 |
| Gene Symbol | TGFBR1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Synonyms | AAT5, ACVRLK4, ALK-5, ALK5, LDS1A, LDS2A, SKR4, TGFR-1 | ||||||||||||||||||||
| Description | transforming growth factor, beta receptor 1 | ||||||||||||||||||||
| Transcript | NM_001130916 LinkOut: [ RefSeq ] | ||||||||||||||||||||
| Other Transcripts | NM_004612 | ||||||||||||||||||||
| Expression | LinkOut: [ BioGPS ] | ||||||||||||||||||||
| KEGG Pathway |
hsa04010 MAPK signaling pathway - Homo sapiens (human) hsa04060 Cytokine-cytokine receptor interaction - Homo sapiens (human) hsa04144 Endocytosis - Homo sapiens (human) hsa04350 TGF-beta signaling pathway - Homo sapiens (human) hsa04520 Adherens junction - Homo sapiens (human) hsa05142 Chagas disease - Homo sapiens (human) hsa05200 Pathways in cancer - Homo sapiens (human) hsa05210 Colorectal cancer - Homo sapiens (human) hsa05212 Pancreatic cancer - Homo sapiens (human) hsa05220 Chronic myeloid leukemia - Homo sapiens (human) |
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| Putative miRNA Targets on TGFBR1 | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] | ||||||||||||||||||||
| 3'UTR of TGFBR1 (miRNA target sites are highlighted) |
>TGFBR1|NM_001130916|3'UTR 1 TAATTCTACAGCTTTGCCTGAACTCTCCTTTTTTCTTCAGATCTGCTCCTGGGTTTTAATTTGGGAGGTCAATTGTTCTA 81 CCTCACTGAGAGGGAACAGAAGGATATTGCTTCCTTTTGCAGCAGTGTAATAAAGTCAATTAAAAACTTCCCAGGATTTC 161 TTTGGACCCAGGAAACAGCCATGTGGGTCCTTTCTGTGCACTATGAACGCTTCTTTCCCAGGACAGAAAATGTGTAGTCT 241 ACCTTTATTTTTTATTAACAAAACTTGTTTTTTAAAAAGATGATTGCTGGTCTTAACTTTAGGTAACTCTGCTGTGCTGG 321 AGATCATCTTTAAGGGCAAAGGAGTTGGATTGCTGAATTACAATGAAACATGTCTTATTACTAAAGAAAGTGATTTACTC 401 CTGGTTAGTACATTCTCAGAGGATTCTGAACCACTAGAGTTTCCTTGATTCAGACTTTGAATGTACTGTTCTATAGTTTT 481 TCAGGATCTTAAAACTAACACTTATAAAACTCTTATCTTGAGTCTAAAAATGACCTCATATAGTAGTGAGGAACATAATT 561 CATGCAATTGTATTTTGTATACTATTATTGTTCTTTCACTTATTCAGAACATTACATGCCTTCAAAATGGGATTGTACTA 641 TACCAGTAAGTGCCACTTCTGTGTCTTTCTAATGGAAATGAGTAGAATTGCTGAAAGTCTCTATGTTAAAACCTATAGTG 721 TTTGAATTCAAAAAGCTTATTTATCTGGGTAACCCAAACTTTTTCTGTTTTGTTTTTGGAAGGGTTTTTGTGGTATGTCA 801 TTTGGTATTCTATTCTGAAAATGCCTTTCTCCTACCAAAATGTGCTTAAGCCACTAAAGAAATGAAGTGGCATTAATTAG 881 TAAATTATTAGCATGGTCATGTTTGAATATTCTCACATCAAGCTTTTGCATTTTAATTGTGTTGTCTAAGTATACTTTTA 961 AAAAATCAAGTGGCACTCTAGATGCTTATAGTACTTTAATATTTGTAGCATACAGACTAATTTTTCTAAAAGGGAAAGTC 1041 TGTCTAGCTGCTTGTGAAAAGTTATGTGGTATTCTGTAAGCCATTTTTTTCTTTATCTGTTCAAAGACTTATTTTTTAAG 1121 ACATGAATTACATTTAAAATTAGAATATGGTTAATATTAAATAATAGGCCTTTTTCTAGGAAGGCGAAGGTAGTTAATAA 1201 TTTGAATAGATAACAGATGTGCAAGAAAGTCACATTTGTTATGTATGTAGGAGTAAACGTTCGGTGGATCCTCTGTCTTT 1281 GTAACTGAGGTTAGAGCTAGTGTGGTTTTGAGGTCTCACTACACTTTGAGGAAGGCAGCTTTTAATTCAGTGTTTCCTTA 1361 TGTGTGCGTACATTGCAACTGCTTACATGTAATTTATGTAATGCATTCAGTGCACCCTTGTTACTTGGGAGAGGTGGTAG 1441 CTAAAGAACATTCTGAGTATAGGTTTTTCTCCATTTACAGATGTCTTTGGTCAAATATTGAAAGCAAACTTGTCATGGTC 1521 TTCTTACATTAAGTTGAAACTAGCTTATAATAACTGGTTTTTACTTCCAATGCTATGAAGTCTCTGCAGGGCTTTTACAG 1601 TTTTCGAAGTCCTTTTATCACTGTGATCTTATTCTGAGGGGAGAAAAAACTATCATAGCTCTGAGGCAAGACTTCGACTT 1681 TATAGTGCTATCAGTTCCCCGATACAGGGTCAGAGTAACCCATACAGTATTTTGGTCAGGAAGAGAAAGTGGCCATTTAC 1761 ACTGAATGAGTTGCATTCTGATAATGTCTTATCTCTTATACGTAGAATAAATTTGAAAGACTATTTGATCTTAAAACCAA 1841 AGTAATTTTAGAATGAGTGACATATTACATAGGAATTTAGTGTCAATTTCATGTGTTTAAAAACATCATGGGAAAAATGC 1921 TTAGAGGTTACTATTTTGACTACAAAGTTGAGTTTTTTTCTGTAGTTACCATAATTTCATTGAAGCAAATGAATGAGTTT 2001 GAGAGGTTTGTTTTTATAGTTGTGTTGTATTACTTGTTTAATAATAATCTCTAATTCTGTGATCAGGTACTTTTTTTGTG 2081 GGGGTTTTTTTTTTGTTTTTTTTTTTTTGTTGTTGTTTTTGGGCCATTTCTAAGCCTACCAGATCTGCTTTATGAAATCC 2161 AGGGGACCAATGCATTTTATCACTAAAACTATTTTTATATAATTTTAAGAATATACCAAAAGTTGTCTGATTTAAAGTTG 2241 TAATACATGATTTCTCACTTTCATGTAAGGTTATCCACTTTTGCTGAAGATATTTTTTATTGAATCAAAGATTGAGTTAC 2321 AATTATACTTTTCTTACCTAAGTGGATAAAATGTACTTTTGATGAATCAGGGAATTTTTTTAAAGTTGGAGTTTAGTTCT 2401 AAATTGACTTTACGTATTACTGCAGTTAATTCCTTTTTTGGCTAGGGATGGTTTGATAAACCACAATTGGCTGATATTGA 2481 AAATGAAAGAAACTTAAAAGGTGGGATGGATCATGATTACTGTCGATAACTGCAGATAAATTTGATTAGAGTAATAATTT 2561 TGTCATTTAAAAACACAGTTGTTTATACTGCCCATCCTAGGATGCTCACCTTCCAAGATTCAACGTGGCTAAAACATCTT 2641 CTGGTAAATTGTGCGTCCATATTCATTTTGTCAGTAGCCAGGAGAAATGGGGATGGGGGAAATACGACTTAGTGAGGCAT 2721 AGACATCCCTGGTCCATCCTTTCTGTCTCCAGCTGTTTCTTGGAACCTGCTCTCCTGCTTGCTGGTCCCTGACGCAGAGA 2801 CCGTTGCCTCCCCCACAGCCGTTTGACTGAAGGCTGCTCTGGAGACCTAGAGTAAAACGGCTGATGGAAGTTGTGGGACC 2881 CACTTCCATTTCCTTCAGTCATTAGAGGTGGAAGGGAGGGGTCTCCAAGTTTGGAGATTGAGCAGATGAGGCTTGGGATG 2961 CCCCTGCTTTGACTTCAGCCATGGATGAGGAGTGGGATGGCAGCAAGGTGGCTCCTGTGGCAGTGGAGTTGTGCCAGAAA 3041 CAGTGGCCAGTTGTATCGCCTATAAGACAGGGTAAGGTCTGAAGAGCTGAGCCTGTAATTCTGCTGTAATAATGATAGTG 3121 CTCAAGAAGTGCCTTGAGTTGGTGTACAGTGCCATGGCCATCAAGAATCCCAGATTTCAGGTTTTATTACAAAATGTAAG 3201 TGGTCACTTGGCGATTTTGTAGTACATGCATGAGTTACCTTTTTTCTCTATGTCTGAGAACTGTCAGATTAAAACAAGAT 3281 GGCAAAGAGATCGTTAGAGTGCACAACAAAATCACTATCCCATTAGACACATCATCAAAAGCTTATTTTTATTCTTGCAC 3361 TGGAAGAATCGTAAGTCAACTGTTTCTTGACCATGGCAGTGTTCTGGCTCCAAATGGTAGTGATTCCAAATAATGGTTCT 3441 GTTAACACTTTGGCAGAAAATGCCAGCTCAGATATTTTGAGATACTAAGGATTATCTTTGGACATGTACTGCAGCTTCTT 3521 GTCTCTGTTTTGGATTACTGGAATACCCATGGGCCCTCTCAAGAGTGCTGGACTTCTAGGACATTAAGATGATTGTCAGT 3601 ACATTAAACTTTTCAATCCCATTATGCAATCTTGTTTGTAAATGTAAACTTCTAAAAATATGGTTAATAACATTCAACCT 3681 GTTTATTACAACTTAAAAGGAACTTCAGTGAATTTGTTTTTATTTTTTAACAAGATTTGTGAACTGAATATCATGAACCA 3761 TGTTTTGATACCCCTTTTTCACGTTGTGCCAACGGAATAGGGTGTTTGATATTTCTTCATATGTTAAGGAGATGCTTCAA 3841 AATGTCAATTGCTTTAAACTTAAATTACCTCTCAAGAGACCAAGGTACATTTACCTCATTGTGTATATAATGTTTAATAT 3921 TTGTCAGAGCATTCTCCAGGTTTGCAGTTTTATTTCTATAAAGTATGGGTATTATGTTGCTCAGTTACTCAAATGGTACT 4001 GTATTGTTTATATTTGTACCCCAAATAACATCGTCTGTACTTTCTGTTTTCTGTATTGTATTTGTGCAGGATTCTTTAGG 4081 CTTTATCAGTGTAATCTCTGCCTTTTAAGATATGTACAGAAAATGTCCATATAAATTTCCATTGAAGTCGAATGATACTG 4161 AGAAGCCTGTAAAGAGGAGAAAAAAACATAAGCTGTGTTTCCCCATAAGTTTTTTTAAATTGTATATTGTATTTGTAGTA 4241 ATATTCCAAAAGAATGTAAATAGGAAATAGAAGAGTGATGCTTATGTTAAGTCCTAACACTACAGTAGAAGAATGGAAGC 4321 AGTGCAAATAAATTACATTTTTCCCAAGTGCCAGTGGCATATTTTAAAATAAAGTGTATACGTTGGAATGAGTCATGCCA 4401 TATGTAGTTGCTGTAGATGGCAACTAGAACCTTTGAGTTACAAGAGTCTTTAGAAGTTTTCTAACCCTGCCTAGTGCAAG 4481 TTACAATATTATAGCGTGTTCGGGGAGTGCCCTCCTGTCTGCAGGTGTGTCTCTGTGCCTGGGGGCTTTTCTCCACATGC 4561 TTAGGGGTGTGGGTCTTCCATTGGGGCATGATGGACCTGTCTACAGGTGATCTCTGTTGCCTTTGGGTCAGCACATTTGT 4641 TAGTCTCCTGGGGGTGAAAACTTGGCTTACAAGAGAACTGGAAAAATGATGAGATGTGGTCCCCAAACCCTTGATTGACT 4721 CTGGGGAGGGGCTTTGTGAATAGGATTGCTCTCACATTAAAGATAGTTACTTCAATTTGAAGGCTGGATTTAGGGATTTT 4801 TTTTTTTCCTTATAACAAAGACATCACCAGGATATGAAGCTTTTGTTGAAAGTTGGAAAAAAAGTGAAATTAAAGACATT 4881 CCCAGACAAA Target sites Provided by authors Predicted by miRanda |
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| miRNA-target interactions (Predicted by miRanda) |
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| miRNA:Target | hsa-miR-204 :: TGFBR1 [ Non-Functional MTI ] |
|---|---|
| Validation Method | Luciferase reporter assay |
| Conditions | HEK293 |
| Location of target site | 3'UTR |
| Tools used in this research | TargetScan |
| Original Description (Extracted from the article) | ... The native seed binding sequence was changed by either 3 (M3) or 7 (M7) nucleotides as indicated in Fig. 10C. The data summarized in Fig. 10D show that there is no signii??cant difference (n 3; P0.77) in luciferase activity between the control (empty vector) and the TGF- R2 mutant construct (M7). Likewise, there is no signii??cant difference (n 3; P0.8) in luciferase activity between the control (empty vector) and the SNAIL2 mutant construct (SNAIL2 M3). Taken together, these data show that TGF- R2 and SNAIL2 are direct targets of miR-204 ... - Wang, F. E. Zhang, C. Maminishkis, A. Dong, et al., 2010, FASEB J. |
| Article |
- Wang, F. E.
Zhang, C.
Maminishkis, A.
Dong, et al. - FASEB J, 2010
MicroRNA (miRNA) expression in fetal human retinal pigment epithelium (hfRPE), retina, and choroid were pairwise compared to determine those miRNAs that are enriched by 10-fold or more in each tissue compared with both of its neighbors. miRs-184, 187, 200a/200b, 204/211, and 221/222 are enriched in hfRPE by 10- to 754-fold compared with neuroretina or choroid (P<0.05). Five of these miRNAs are enriched in RPE compared with 20 tissues throughout the body and are 10- to 20,000-fold more highly expressed (P<0.005). miR-204 and 211 are the most highly expressed in the RPE. In addition, expression of miR-204/211 is significantly lower in the NCI60 tumor cell line panel compared with that in 13 normal tissues, suggesting the progressive disruption of epithelial barriers and increased proliferation. We demonstrated that TGF-beta receptor 2 (TGF-betaR2) and SNAIL2 are direct targets of miR-204 and that a reduction in miR-204 expression leads to reduced expression of claudins 10, 16, and 19 (message/protein) consistent with our observation that anti-miR-204/211 decreased transepithelial resistance by 80% and reduced cell membrane voltage and conductance. The anti-miR-204-induced decrease in Kir7.1 protein levels suggests a signaling pathway that connects TGF-betaR2 and maintenance of potassium homeostasis. Overall, these data indicate a critical role for miR-204/211 in maintaining epithelial barrier function and cell physiology.-Wang, F. E., Zhang, C., Maminishkis, A., Dong, L., Zhi, C., Li, R., Zhao, J., Majerciak, V., Gaur, A. B., Chen, S., Miller, S. S. MicroRNA-204/211 alters epithelial physiology.
LinkOut: [PMID: 20056717]
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