Accession ID: MIRT003274 [miRNA, hsa-miR-30d :: GNAI2, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-30d LinkOut: [miRBase ]
Description Homo sapiens miR-30d stem-loop
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-30d-3p
Mature Sequence 46| CUUUCAGUCAGAUGUUUGCUGC |67
Evidence Experimental
Experiments Cloned
Putative hsa-miR-30d-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-30d-5p
Mature Sequence 6| UGUAAACAUCCCCGACUGGAAG |27
Evidence Experimental
Experiments Cloned
Putative hsa-miR-30d-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol GNAI2 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
Description guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Transcript NM_001166425   LinkOut: [ RefSeq ]
Other Transcripts NM_002070   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04062    Chemokine signaling pathway - Homo sapiens (human)
hsa04360    Axon guidance - Homo sapiens (human)
hsa04530    Tight junction - Homo sapiens (human)
hsa04540    Gap junction - Homo sapiens (human)
hsa04670    Leukocyte transendothelial migration - Homo sapiens (human)
hsa04730    Long-term depression - Homo sapiens (human)
hsa04914    Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa04916    Melanogenesis - Homo sapiens (human)
hsa05142    Chagas disease - Homo sapiens (human)
Putative miRNA Targets on GNAI2 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of GNAI2
(miRNA target sites are highlighted)		 
>GNAI2|NM_001166425|3'UTR
   1 TGAGGGGCAGCGGGGCCTGGCGGGATGGGCCACCGCCGACTTTGTACCCCCCAACCCCTGAGGAAGATGGGGGCAAGAAG
  81 ATCACGCTCCCCGCCTGTTCCCCCGCCGCTTTTCTCCTCTTTCCTCTCTTTGTTCTCAGCTCCCCCTGTCCCCTCAGCTC
 161 CAGACGTAGGGGAGGGGTTGCCACAGGCCTCCCTGTTTGAAGCCTGCCCTTGTCTGAGATGCTGGTAATGGCCATGGTAC
 241 CCCCTTCTGGGCATCTGTTCTGGTTTTTAACCATTGTCTTGTTCTGTGATGAGGGGAGGGGGGCACATGCTGAGTCTCCC
 321 AAGGCTGCGTCTGGAGGGGCCCCTGCTTCTCCAGCCTGGACCCCCAGCTTTGCCCAACACCAGCCCCTGCCCCAGCCCAA
 401 GTCCAAATGTTTACAGGGAGCCTCCTGCCCAGTCCCCCAACCCCAGCCGCTCGGAGGCCCCAAAGGAAAAAGCACAAGAA
 481 GCGTGAGACGCCACCATTCCTGGAAACCACAGTCCACCTGCTCATTCTCGTAGCTTTTTAAAAAAATGAAAGTAAAGGAA
 561 AAAAAAAAAACTGCAAATCTAGAAAACTTTTTAGAGAAAAACTATTTAAAACTGTCAGATCCTGACCAGCAAGCCCCCCC
 641 CCAGCCCCCCTTCCAAGTGACTCCGTGCCTTGAGTGTGTCTGCGTGTTTACACCCGTCCCTCTGCTGGCCGCCCCCGTGC
 721 GAGCGGCACCCCTGCCCTGCCCTCCACAGAATTGGGTTCCAAGGGCTGTTCCAGACAACTGCCAACGTCACTGAGGGCCC
 801 TGCCCCAGCGGCCCTGGCCCCAGGCTCTATTAACCTAAAATGTAGCTCCCTAGCGCTAACCTAGGAACCGCCGCTGCCTG
 881 CTGGGGGGCCACGCCCCTCATGCCCTTGTCCCAGGCCCGGGGCCTTCAGCGTTGAACACTTCCTTGCTTTTTTCACATGT
 961 TTTATGGAATTGTTCACCTGGTTTGAAATAATAAAATGTAGAAAGAAAAAAAAT
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' cguCGUUUGUAGACUGACUUUc 5'
             || |||:||  |||||:: 
Target 5' actGCCAACGTC--ACTGAGGg 3'
 		778 - 797 131.00 -17.30
2 
miRNA  3' cgUCGUUUGUAGACUGACUUUc 5'
            |||  :|  :|| ||||:| 
Target 5' gaAGCCTGCCCTTGTCTGAGAt 3'
 		199 - 220 112.00 -11.80
3 
miRNA  3' cgucGUUUGUAGACUGACUUUc 5'
              :||| |    | ||||| 
Target 5' ttttTAAAAA----AATGAAAg 3'
 		535 - 552 112.00 -7.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-30d :: GNAI2    [ Functional MTI ]
Validation Method Western blot
Conditions HEK293T
Location of target site 3'UTR
Tools used in this research microRNA.org, miRanda, PicTar, TargetScan
Original Description (Extracted from the article) ... miR-30d Posttranscriptionally Down-regulates GNAI2 Expression by Directly Targeting Its 3`-UTR. ...

- Yao, J. Liang, L. Huang, S. Ding, J. Tan, et al., 2010, Hepatology.
miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1 
miRNA  3' gaagguCAGCCCCUACAAAUGu 5'
                ||| | | ||||||| 
Target 5' gaguguGUCUGCG-UGUUUACa 3'
 		3 - 23
Article - Yao, J. Liang, L. Huang, S. Ding, J. Tan, et al.
- Hepatology, 2010
The pathological relevance and significance of microRNAs (miRNAs) in hepatocarcinogenesis have attracted much attention in recent years; however, little is known about the underlying molecular mechanisms through which miRNAs are involved in the development and progression of hepatocellular carcinoma (HCC). In this study, we demonstrate that miR-30d is frequently up-regulated in HCC and that its expression is highly associated with the intrahepatic metastasis of HCC. Furthermore, the enhanced expression of miR-30d could promote HCC cell migration and invasion in vitro and intrahepatic and distal pulmonary metastasis in vivo, while silencing its expression resulted in a reduced migration and invasion. Galphai2 (GNAI2) was identified as the direct and functional target of miR-30d with integrated bioinformatics analysis and messenger RNA array assay. This regulation was further confirmed by luciferase reporter assays. In addition, our results, for the first time, showed that GNAI2 was frequently suppressed in HCC by way of quantitative reverse-transcription polymerase chain reaction and immunohistochemical staining assays. The increase of the GNAI2 expression significantly inhibits, whereas knockdown of the GNAI2 expression remarkably enhances HCC cell migration and invasion, indicating that GNAI2 functions as a metastasis suppressor in HCC. The restoration of GNAI2 can inhibit miR-30d-induced HCC cell invasion and metastasis. Conclusion: The newly identified miR-30d/GNAI2 axis elucidates the molecular mechanism of HCC cell invasion and metastasis and represents a new potential therapeutic target for HCC treatment. (HEPATOLOGY 2010.).
LinkOut: [PMID: 20054866]