Accession ID: MIRT003281 [miRNA, hsa-miR-141-3p :: PTEN, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-141LinkOut: [miRBase ]
Description Homo sapiens miR-141 stem-loop
Comment This miRNA sequence was predicted based on homology to a verified miRNA from mouse .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-141-3p
Mature Sequence 59| UAACACUGUCUGGUAAAGAUGG |80
Evidence Experimental
Experiments Cloned
Putative hsa-miR-141-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol PTEN LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms 10q23del, BZS, DEC, GLM2, MHAM, MMAC1, PTEN1, TEP1
Description phosphatase and tensin homolog
Transcript NM_0003    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on PTEN LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of PTEN
(miRNA target sites are highlighted)
>PTEN|NM_0003|3'UTR
   1 ATTTTTTTTTATCAAGAGGGATAAAACACCATGAAAATAAACTTGAATAAACTGAAAATGGACCTTTTTTTTTTTAATGG
  81 CAATAGGACATTGTGTCAGATTACCAGTTATAGGAACAATTCTCTTTTCCTGACCAATCTTGTTTTACCCTATACATCCA
 161 CAGGGTTTTGACACTTGTTGTCCAGTTGAAAAAAGGTTGTGTAGCTGTGTCATGTATATACCTTTTTGTGTCAAAAGGAC
 241 ATTTAAAATTCAATTAGGATTAATAAAGATGGCACTTTCCCGTTTTATTCCAGTTTTATAAAAAGTGGAGACAGACTGAT
 321 GTGTATACGTAGGAATTTTTTCCTTTTGTGTTCTGTCACCAACTGAAGTGGCTAAAGAGCTTTGTGATATACTGGTTCAC
 401 ATCCTACCCCTTTGCACTTGTGGCAACAGATAAGTTTGCAGTTGGCTAAGAGAGGTTTCCGAAGGGTTTTGCTACATTCT
 481 AATGCATGTATTCGGGTTAGGGGAATGGAGGGAATGCTCAGAAAGGAAATAATTTTATGCTGGACTCTGGACCATATACC
 561 ATCTCCAGCTATTTACACACACCTTTCTTTAGCATGCTACAGTTATTAATCTGGACATTCGAGGAATTGGCCGCTGTCAC
 641 TGCTTGTTGTTTGCGCATTTTTTTTTAAAGCATATTGGTGCTAGAAAAGGCAGCTAAAGGAAGTGAATCTGTATTGGGGT
 721 ACAGGAATGAACCTTCTGCAACATCTTAAGATCCACAAATGAAGGGATATAAAAATAATGTCATAGGTAAGAAACACAGC
 801 AACAATGACTTAACCATATAAATGTGGAGGCTATCAACAAAGAATGGGCTTGAAACATTATAAAAATTGACAATGATTTA
 881 TTAAATATGTTTTCTCAATTGTAACGACTTCTCCATCTCCTGTGTAATCAAGGCCAGTGCTAAAATTCAGATGCTGTTAG
 961 TACCTACATCAGTCAACAACTTACACTTATTTTACTAGTTTTCAATCATAATACCTGCTGTGGATGCTTCATGTGCTGCC
1041 TGCAAGCTTCTTTTTTCTCATTAAATATAAAATATTTTGTAATGCTGCACAGAAATTTTCAATTTGAGATTCTACAGTAA
1121 GCGTTTTTTTTCTTTGAAGATTTATGATGCACTTATTCAATAGCTGTCAGCCGTTCCACCCTTTTGACCTTACACATTCT
1201 ATTACAATGAATTTTGCAGTTTTGCACATTTTTTAAATGTCATTAACTGTTAGGGAATTTTACTTGAATACTGAATACAT
1281 ATAATGTTTATATTAAAAAGGACATTTGTGTTAAAAAGGAAATTAGAGTTGCAGTAAACTTTCAATGCTGCACACAAAAA
1361 AAAGACATTTGATTTTTCAGTAGAAATTGTCCTACATGTGCTTTATTGATTTGCTATTGAAAGAATAGGGTTTTTTTTTT
1441 TTTTTTTTTTTTTTTTTTTAAATGTGCAGTGTTGAATCATTTCTTCATAGTGCTCCCCCGAGTTGGGACTAGGGCTTCAA
1521 TTTCACTTCTTAAAAAAAATCATCATATATTTGATATGCCCAGACTGCATACGATTTTAAGCGGAGTACAACTACTATTG
1601 TAAAGCTAATGTGAAGATATTATTAAAAAGGTTTTTTTTTCCAGAAATTTGGTGTCTTCAAATTATACCTTCACCTTGAC
1681 ATTTGAATATCCAGCCATTTTGTTTCTTAATGGTATAAAATTCCATTTTCAATAACTTATTGGTGCTGAAATTGTTCACT
1761 AGCTGTGGTCTGACCTAGTTAATTTACAAATACAGATTGAATAGGACCTACTAGAGCAGCATTTATAGAGTTTGATGGCA
1841 AATAGATTAGGCAGAACTTCATCTAAAATATTCTTAGTAAATAATGTTGACACGTTTTCCATACCTTGTCAGTTTCATTC
1921 AACAATTTTTAAATTTTTAACAAAGCTCTTAGGATTTACACATTTATATTTAAACATTGATATATAGAGTATTGATTGAT
2001 TGCTCATAAGTTAAATTGGTAAAGTTAGAGACAACTATTCTAACACCTCACCATTGAAATTTATATGCCACCTTGTCTTT
2081 CATAAAAGCTGAAAATTGTTACCTAAAATGAAAATCAACTTCATGTTTTGAAGATAGTTATAAATATTGTTCTTTGTTAC
2161 AATTTCGGGCACCGCATATTAAAACGTAACTTTATTGTTCCAATATGTAACATGGAGGGCCAGGTCATAAATAATGACAT
2241 TATAATGGGCTTTTGCACTGTTATTATTTTTCCTTTGGAATGTGAAGGTCTGAATGAGGGTTTTGATTTTGAATGTTTCA
2321 ATGTTTTTGAGAAGCCTTGCTTACATTTTATGGTGTAGTCATTGGAAATGGAAAAATGGCATTATATATATTATATATAT
2401 AAATATATATTATACATACTCTCCTTACTTTATTTCAGTTACCATCCCCATAGAATTTGACAAGAATTGCTATGACTGAA
2481 AGGTTTTCGAGTCCTAATTAAAACTTTATTTATGGCAGTATTCATAATTAGCCTGAAATGCATTCTGTAGGTAATCTCTG
2561 AGTTTCTGGAATATTTTCTTAGACTTTTTGGATGTGCAGCAGCTTACATGTCTGAAGTTACTTGAAGGCATCACTTTTAA
2641 GAAAGCTTACAGTTGGGCCCTGTACCATCCCAAGTCCTTTGTAGCTCCTCTTGAACATGTTTGCCATACTTTTAAAAGGG
2721 TAGTTGAATAAATAGCATCACCATTCTTTGCTGTGGCACAGGTTATAAACTTAAGTGGAGTTTACCGGCAGCATCAAATG
2801 TTTCAGCTTTAAAAAATAAAAGTAGGGTACAAGTTTAATGTTTAGTTCTAGAAATTTTGTGCAATATGTTCATAACGATG
2881 GCTGTGGTTGCCACAAAGTGCCTCGTTTACCTTTAAATACTGTTAATGTGTCATGCATGCAGATGGAAGGGGTGGAACTG
2961 TGCACTAAAGTGGGGGCTTTAACTGTAGTATTTGGCAGAGTTGCCTTCTACCTGCCAGTTCAAAAGTTCAACCTGTTTTC
3041 ATATAGAATATATATACTAAAAAATTTCAGTCTGTTAAACAGCCTTACTCTGATTCAGCCTCTTCAGATACTCTTGTGCT
3121 GTGCAGCAGTGGCTCTGTGTGTAAATGCTATGCACTGAGGATACACAAAAATACCAATATGATGTGTACAGGATAATGCC
3201 TCATCCCAATCAGATGTCCATTTGTTATTGTGTTTGTTAACAACCCTTTATCTCTTAGTGTTATAAACTCCACTTAAAAC
3281 TGATTAAAGTCTCATTCTTGTCA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' gguAGAAAUGGUCUGUCACAAu 5'
             |:|| | :: :||||||| 
Target 5' tttTTTTAAATGTGCAGTGTTg 3'
1453 - 1474 155.00 -11.10
2
miRNA  3' gguaGAAAUGGUCUGUCACAAu 5'
              |||||:|   :|||||| 
Target 5' aaccCTTTATCTCTTAGTGTTa 3'
3242 - 3263 146.00 -16.30
3
miRNA  3' ggUAGAAAU-GGU-CUGUCACAAu 5'
            | ||||| ::| |:|||| || 
Target 5' aaAACTTTATTTATGGCAGTATTc 3'
2500 - 2523 132.00 -10.06
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-141-3p :: PTEN    [ Functional MTI ]
Validation Method Luciferase reporter assay , Western blot , Reporter assay;Western blot;Other
Location of target site 3'UTR
Tools used in this research PicTar , TargetScan
Original Description (Extracted from the article) ... We found BRD3, UBAP1, PTEN are potential targets of miR-141 which had been confirmed through following luciferase reporter assay and western blotting. ...

- Zhang, L. Deng, T. Li, X. Liu, H. Zhou, H. et al., 2010, Carcinogenesis.

Article - Zhang, L. Deng, T. Li, X. Liu, H. Zhou, H. et al.
- Carcinogenesis, 2010
microRNAs (miRNAs) are small non-coding RNAs and have been implicated in the pathology of various diseases, including cancer. Here we report that the miRNA profiles have been changed after knockdown of one of the most important oncogene c-MYC or re-expression of a candidate tumor suppressor gene SPLUNC1 in nasopharyngeal carcinoma (NPC) cells. Both c-MYC knockdown and SPLUNC1 re-expression can down-regulate microRNA-141 (miR-141). miR-141 is up-regulated in NPC specimens in comparison with normal nasopharyngeal epithelium. Inhibition of miR-141 could affect cell cycle, apoptosis, cell growth, migration and invasion in NPC cells. We found that BRD3, UBAP1 and PTEN are potential targets of miR-141, which had been confirmed following luciferase reporter assays and western blotting. BRD3 and UBAP1 are both involved in NPC carcinogenesis as confirmed through our previous studies and PTEN is a crucial tumor suppressor in many tumor types. BRD3 is involved in the regulation of the Rb/E2F pathway. Inhibition of miR-141 could affect some important molecules in the Rb/E2F, JNK2 and AKT pathways. It is well known that carcinogenesis of NPC is involved in the networks of genetic and epigenetic alteration events. We propose that miR-141- and tumor-related genes c-MYC, SPLUNC1, BRD3, UBAP1 and PTEN may constitute a gene-miRNA network to contribute to NPC development.
LinkOut: [PMID: 20053927]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
43 hsa-miR-141-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000316 ZEB2 zinc finger E-box binding homeobox 2 4 5
MIRT000317 ZEB1 zinc finger E-box binding homeobox 1 3 5
MIRT000318 SIP1 gem (nuclear organelle) associated protein 2 1 1
MIRT000320 DLX5 distal-less homeobox 5 2 1
MIRT000727 BAP1 BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) 3 3
MIRT001812 KLF5 Kruppel-like factor 5 (intestinal) 2 1
MIRT001813 STK3 serine/threonine kinase 3 2 1
MIRT002285 TGFB2 transforming growth factor, beta 2 3 1
MIRT002308 HMGB1 high mobility group box 1 1 1
MIRT002309 SFPQ splicing factor proline/glutamine-rich 3 1
MIRT002311 SERBP1 SERPINE1 mRNA binding protein 1 1 1
MIRT002951 CLOCK clock homolog (mouse) 4 2
MIRT003279 BRD3 bromodomain containing 3 3 1
MIRT003280 UBAP1 ubiquitin associated protein 1 3 1
MIRT003281 PTEN phosphatase and tensin homolog 3 1
MIRT004621 ZFPM2 zinc finger protein, multitype 2 3 1
MIRT004868 TRAPPC2P1 trafficking protein particle complex 2 pseudogene 1 2 1
MIRT005479 EIF4E eukaryotic translation initiation factor 4E 4 1
MIRT006173 CTBP2 C-terminal binding protein 2 2 1
MIRT006174 CDYL chromodomain protein, Y-like 2 1
MIRT006342 ACVR2B activin A receptor, type IIB 2 1
MIRT006424 MAPK14 mitogen-activated protein kinase 14 3 1
MIRT006709 PPARA peroxisome proliferator-activated receptor alpha 3 1
MIRT006718 NR0B2 nuclear receptor subfamily 0, group B, member 2 1 1
MIRT006730 YWHAG tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 3 1
MIRT021646 ELAVL4 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 1 1
MIRT021647 MAPK9 mitogen-activated protein kinase 9 1 1
MIRT021648 TFDP2 transcription factor Dp-2 (E2F dimerization partner 2) 1 1
MIRT021649 E2F3 E2F transcription factor 3 1 1
MIRT021650 SHC1 SHC (Src homology 2 domain containing) transforming protein 1 1 1
MIRT021651 VAC14 Vac14 homolog (S. cerevisiae) 1 1
MIRT021652 TCF7L1 transcription factor 7-like 1 (T-cell specific, HMG-box) 1 1
MIRT021653 ELMO2 engulfment and cell motility 2 2 1
MIRT021654 RASSF2 Ras association (RalGDS/AF-6) domain family member 2 1 1
MIRT021655 KLHL20 kelch-like 20 (Drosophila) 2 1
MIRT021656 RIN2 Ras and Rab interactor 2 1 1
MIRT021657 SEPT7 septin 7 1 1
MIRT021658 HOXB5 homeobox B5 1 1
MIRT021659 ERBB2IP erbb2 interacting protein 2 1
MIRT021660 KLF11 Kruppel-like factor 11 1 1
MIRT021661 PTPRD protein tyrosine phosphatase, receptor type, D 2 1
MIRT021662 WDR37 WD repeat domain 37 2 1
MIRT035576 MAP2K4 mitogen-activated protein kinase kinase 4 1 1