Accession ID: MIRT003289 [miRNA, hsa-miR-29c-3p :: BCL2, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-29cLinkOut: [miRBase ]
Description Homo sapiens miR-29c stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-29c-3p
Mature Sequence 54| UAGCACCAUUUGAAAUCGGUUA |75
Evidence Experimental
Experiments Cloned
Putative hsa-miR-29c-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms
Description
Transcript    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of
(miRNA target sites are highlighted)
>||3'UTR
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-29c-3p :: BCL2    [ Functional MTI ]
Validation Method Luciferase reporter assay , qRT-PCR , Western blot , Other
Conditions HEK293T
Location of target site 3'UTR
Tools used in this research miRanda , TargetScan
Original Description (Extracted from the article) ... Bcl-2 and Mcl-1 Are Direct Targets of miR-29. ...

- Xiong, Y. Fang, J. H. Yun, J. P. Yang, J. et al., 2010, Hepatology.

Article - Xiong, Y. Fang, J. H. Yun, J. P. Yang, J. et al.
- Hepatology, 2010
Based on microarray data, we have previously shown a significant down-regulation of miR-29 in hepatocellular carcinoma (HCC) tissues. To date, the role of miR-29 deregulation in hepatocarcinogenesis and the signaling pathways by which miR-29 exerts its function and modulates the malignant phenotypes of HCC cells remain largely unknown. In this study, we confirmed that reduced expression of miR-29 was a frequent event in HCC tissues using both Northern blot and real-time quantitative reverse-transcription polymerase chain reaction. More interestingly, we found that miR-29 down-regulation was significantly associated with worse disease-free survival of HCC patients. Both gain- and loss-of-function studies revealed that miR-29 could sensitize HCC cells to apoptosis that was triggered by either serum starvation and hypoxia or chemotherapeutic drugs, which mimicked the tumor growth environment in vivo and the clinical treatment. Moreover, introduction of miR-29 dramatically repressed the ability of HCC cells to form tumor in nude mice. Subsequent investigation characterized two antiapoptotic molecules, Bcl-2 and Mcl-1, as direct targets of miR-29. Furthermore, silencing of Bcl-2 and Mcl-1 phenocopied the proapoptotic effect of miR-29, whereas overexpression of these proteins attenuated the effect of miR-29. In addition, enhanced expression of miR-29 resulted in the loss of mitochondrial potential and the release of cytochrome c to cytoplasm, suggesting that miR-29 may promote apoptosis through a mitochondrial pathway that involves Mcl-1 and Bcl-2. Conclusion: Our data highlight an important role of miR-29 in the regulation of apoptosis and in the molecular etiology of HCC, and implicate the potential application of miR-29 in prognosis prediction and in cancer therapy.
LinkOut: [PMID: 20041405]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
107 hsa-miR-29c-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000927 COL3A1 collagen, type III, alpha 1 2 1
MIRT000928 COL1A1 collagen, type I, alpha 1 3 2
MIRT001921 TDG thymine-DNA glycosylase 2 1
MIRT001922 SPARC secreted protein, acidic, cysteine-rich (osteonectin) 3 2
MIRT001923 LAMC1 laminin, gamma 1 (formerly LAMB2) 2 1
MIRT001925 COL15A1 collagen, type XV, alpha 1 3 1
MIRT001926 COL4A2 collagen, type IV, alpha 2 2 1
MIRT001927 COL4A1 collagen, type IV, alpha 1 3 2
MIRT001928 COL1A2 collagen, type I, alpha 2 3 2
MIRT001929 GAPDH glyceraldehyde-3-phosphate dehydrogenase 2 1
MIRT003025 DNMT3B DNA (cytosine-5-)-methyltransferase 3 beta 4 1
MIRT003028 DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha 5 3
MIRT003200 CDK6 cyclin-dependent kinase 6 4 1
MIRT003288 MCL1 myeloid cell leukemia sequence 1 (BCL2-related) 6 3
MIRT003289 BCL2 B-cell CLL/lymphoma 2 4 1
MIRT003829 FBN1 fibrillin 1 3 1
MIRT003994 SRSF10 serine/arginine-rich splicing factor 10 2 1
MIRT005375 Col6a2 collagen, type VI, alpha 2 3 1
MIRT005376 Ctsk cathepsin K 3 1
MIRT005382 Mmp15 matrix metallopeptidase 15 3 1
MIRT005384 MMP15 matrix metallopeptidase 15 (membrane-inserted) 2 1
MIRT005386 MMP24 matrix metallopeptidase 24 (membrane-inserted) 3 1
MIRT005388 Mmp24 matrix metallopeptidase 24 2 1
MIRT005525 FGG fibrinogen gamma chain 2 1
MIRT005526 FGB fibrinogen beta chain 2 1
MIRT005527 FGA fibrinogen alpha chain 2 1
MIRT006382 COL7A1 collagen, type VII, alpha 1 1 1
MIRT006383 COL21A1 collagen, type XXI, alpha 1 1 1
MIRT006384 TFAP2C transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) 1 1
MIRT006385 WNT4 wingless-type MMTV integration site family, member 4 1 1
MIRT006386 IGFBP1 insulin-like growth factor binding protein 1 1 1
MIRT006487 PPP1R13B protein phosphatase 1, regulatory subunit 13B 2 1
MIRT006489 CDC42 cell division cycle 42 (GTP binding protein, 25kDa) 3 1
MIRT020357 PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) 1 1
MIRT020358 MKL2 MKL/myocardin-like 2 1 1
MIRT020359 C12orf76 chromosome 12 open reading frame 76 1 1
MIRT020360 NUMB numb homolog (Drosophila) 1 1
MIRT020361 ZNF45 zinc finger protein 45 1 1
MIRT020362 SH3GLB1 SH3-domain GRB2-like endophilin B1 1 1
MIRT020363 IRF2BP2 interferon regulatory factor 2 binding protein 2 1 1
MIRT020364 WSB2 WD repeat and SOCS box containing 2 1 1
MIRT020365 PURG purine-rich element binding protein G 1 1
MIRT020366 CDV3 CDV3 homolog (mouse) 1 1
MIRT020367 JUN jun proto-oncogene 1 1
MIRT020368 TIPRL TIP41, TOR signaling pathway regulator-like (S. cerevisiae) 1 1
MIRT020369 OIP5 Opa interacting protein 5 1 1
MIRT020370 SCAF11 SR-related CTD-associated factor 11 1 1
MIRT020371 GNB4 guanine nucleotide binding protein (G protein), beta polypeptide 4 1 1
MIRT020372 R3HDM4 R3H domain containing 4 1 1
MIRT020373 ADO 2-aminoethanethiol (cysteamine) dioxygenase 1 1
MIRT020374 FAM102B family with sequence similarity 102, member B 1 1
MIRT020375 KCTD15 potassium channel tetramerisation domain containing 15 1 1
MIRT020376 U2SURP U2 snRNP-associated SURP domain containing 1 1
MIRT020377 KLHL28 kelch-like 28 (Drosophila) 1 1
MIRT020378 BFAR bifunctional apoptosis regulator 1 1
MIRT020379 CDC42SE1 CDC42 small effector 1 1 1
MIRT020380 PPP1R14C protein phosphatase 1, regulatory (inhibitor) subunit 14C 1 1
MIRT020381 HMGCR 3-hydroxy-3-methylglutaryl-CoA reductase 1 1
MIRT020382 SOWAHC sosondowah ankyrin repeat domain family member C 1 1
MIRT020383 TXNDC16 thioredoxin domain containing 16 1 1
MIRT020384 ELMSAN1 ELM2 and Myb/SANT-like domain containing 1 1 1
MIRT020385 IRGQ immunity-related GTPase family, Q 1 1
MIRT020386 PDHX pyruvate dehydrogenase complex, component X 1 1
MIRT020387 CEP68 centrosomal protein 68kDa 1 1
MIRT020388 NKIRAS2 NFKB inhibitor interacting Ras-like 2 1 1
MIRT020389 VEGFA vascular endothelial growth factor A 1 1
MIRT020390 ZBTB10 zinc finger and BTB domain containing 10 1 1
MIRT020391 AKT3 v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) 1 1
MIRT020392 SPIN4 spindlin family, member 4 1 1
MIRT020393 KIAA0895 KIAA0895 1 1
MIRT020394 CDC23 cell division cycle 23 homolog (S. cerevisiae) 1 1
MIRT020395 DDX21 DEAD (Asp-Glu-Ala-Asp) box helicase 21 1 1
MIRT020396 BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) 1 1
MIRT020397 CCNT2 cyclin T2 1 1
MIRT020398 DICER1 dicer 1, ribonuclease type III 1 1
MIRT020399 FREM2 FRAS1 related extracellular matrix protein 2 1 1
MIRT020400 TMTC3 transmembrane and tetratricopeptide repeat containing 3 1 1
MIRT020401 AMER1 family with sequence similarity 123B 1 1
MIRT020402 KIAA1549 KIAA1549 1 1
MIRT020403 DCAF12 DDB1 and CUL4 associated factor 12 1 1
MIRT020404 ANKRD13C ankyrin repeat domain 13C 1 1
MIRT020405 HDGF hepatoma-derived growth factor 1 1
MIRT020406 MORF4L1 mortality factor 4 like 1 1 1
MIRT020407 PLAG1 pleiomorphic adenoma gene 1 1 1
MIRT020408 RAB30 RAB30, member RAS oncogene family 1 1
MIRT020409 SUV420H2 suppressor of variegation 4-20 homolog 2 (Drosophila) 1 1
MIRT020410 FAM105B family with sequence similarity 105, member B 1 1
MIRT020411 PRKAB2 protein kinase, AMP-activated, beta 2 non-catalytic subunit 1 1
MIRT020412 KDM5B lysine (K)-specific demethylase 5B 1 1
MIRT020413 BACH2 BTB and CNC homology 1, basic leucine zipper transcription factor 2 1 1
MIRT020414 RNF19A ring finger protein 19A, E3 ubiquitin protein ligase 1 1
MIRT020415 MYCN v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) 1 1
MIRT020416 BACE1 beta-site APP-cleaving enzyme 1 3 1
MIRT044232 MMS19 MMS19 nucleotide excision repair homolog (S. cerevisiae) 1 1
MIRT044233 C15orf52 chromosome 15 open reading frame 52 1 1
MIRT044234 MTHFD2 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase 1 1
MIRT044235 CTC1 CTS telomere maintenance complex component 1 1 1
MIRT044236 XK X-linked Kx blood group (McLeod syndrome) 1 1
MIRT044237 TIMM44 translocase of inner mitochondrial membrane 44 homolog (yeast) 1 1
MIRT044238 PSMA2 proteasome (prosome, macropain) subunit, alpha type, 2 1 1
MIRT044239 CHIC2 cysteine-rich hydrophobic domain 2 1 1
MIRT044240 HUWE1 HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase 1 1
MIRT044241 STK19 serine/threonine kinase 19 1 1
MIRT044242 PPT1 palmitoyl-protein thioesterase 1 1 1
MIRT044243 BCKDHA branched chain keto acid dehydrogenase E1, alpha polypeptide 1 1
MIRT044244 PPM1D protein phosphatase, Mg2+/Mn2+ dependent, 1D 1 1
MIRT044245 FAM126B family with sequence similarity 126, member B 1 1