Accession ID: MIRT003333 [miRNA, hsa-miR-15a :: BRCA1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-15a LinkOut: [miRBase ]
Synonyms MIRN15A, hsa-mir-15a, miRNA15A, MIR15A
Description Homo sapiens miR-15a stem-loop
Comment Reference .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-15a-3p
Mature Sequence 51| CAGGCCAUAUUGUGCUGCCUCA |72
Evidence Experimental
Experiments Cloned
Putative hsa-miR-15a-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-15a-5p
Mature Sequence 14| UAGCAGCACAUAAUGGUUUGUG |35
Evidence Experimental
Experiments Cloned
Putative hsa-miR-15a-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol BRCA1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PSCP, RNF53
Description breast cancer 1, early onset
Transcript NM_007299   LinkOut: [ RefSeq ]
Other Transcripts NM_007297, NM_007298, NM_007294, NM_007300   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04120    Ubiquitin mediated proteolysis - Homo sapiens (human)
Putative miRNA Targets on BRCA1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of BRCA1
(miRNA target sites are highlighted)		 
>BRCA1|NM_007299|3'UTR
   1 TGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGA
  81 TACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACA
 161 AAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACA
 241 TCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATG
 321 AGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTC
 401 ATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAG
 481 AATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAG
 561 AGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGA
 641 GGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCAC
 721 TTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCT
 801 ACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATC
 881 ACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGG
 961 CTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAA
1041 AGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGT
1121 AAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCT
1201 ATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAA
1281 CCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTAT
1361 AGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTT
1441 GTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCAAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' acuccgUCG--UGUUAUACCGGAc 5'
                |||  |||| :|||||| 
Target 5' agaatgAGCTTACAAAGTGGCCTt 3'
 		147 - 170 146.00 -16.50
2 
miRNA  3' acuCCGUCGUGUUAUACCGGAc 5'
             || ||||||: :||||:| 
Target 5' gctGGAAGCACAGAGTGGCTTg 3'
 		449 - 470 143.00 -23.40
3 
miRNA  3' acuccgUCGUGUU---AUACCGgac 5'
                |||||||   |||||:   
Target 5' gccatgAGCACAAAATTATGGTaat 3'
 		315 - 339 112.00 -11.92
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-15a :: BRCA1    [ Functional MTI ]
Validation Method Luciferase reporter assay
Conditions 293T
Location of target site 3'UTR
Tools used in this research miRanda
miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1 
miRNA  3' guGUUUGGUAAUACACGACGAu 5'
            :||| ::|  |||||||:| 
Target 5' ugUAAA-UGU--UGUGCUGUUa 3'
 		4 - 22
Article - Zhu, J. Y. Pfuhl, T. Motsch, N. Barth, S. et al.
- J Virol, 2009
MicroRNAs (miRNAs) represent a conserved class of small noncoding RNAs that are found in all higher eukaryotes as well as some DNA viruses. miRNAs are 20 to 25 nucleotides in length and have important regulatory functions in biological processes such as embryonic development, cell differentiation, hormone secretion, and metabolism. Furthermore, miRNAs have been implicated in the pathology of various diseases, including cancer. miRNA expression profiles not only classify different types of cancer but also may even help to characterize distinct tumor stages, therefore constituting a valuable tool for prognosis. Here we report the miRNA profile of Epstein-Barr virus (EBV)-positive nasopharyngeal carcinoma (NPC) tissue samples characterized by cloning and sequencing. We found that all EBV miRNAs from the BART region are expressed in NPC tissues, whereas EBV miRNAs from the BHRF1 region are not found. Moreover, we identified two novel EBV miRNA genes originating from the BART region that have not been found in other tissues or cell lines before. We also identified three new human miRNAs which might be specific for nasopharyngeal tissues. We further show that a number of different cellular miRNAs, including miR-15a and miR-16, are up- or downregulated in NPC tissues compared to control tissues. We found that the tumor suppressor BRCA-1 is a target of miR-15a as well as miR-16, suggesting a miRNA role in NPC pathogenesis.
LinkOut: [PMID: 19144710]