Accession ID: MIRT003393 [miRNA, hsa-miR-224-5p :: CD40, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-224LinkOut: [miRBase ]
Description Homo sapiens miR-224 stem-loop
Comment This miR was identified and ends mapped by cloning from Weri cells in human. The sequence maps to chromosome X. An erratum corrected the originally published name miR-175 to miR-224. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-224-5p
Mature Sequence 8| CAAGUCACUAGUGGUUCCGUU |28
Evidence Experimental
Experiments Cloned
Putative hsa-miR-224-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol CD40 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms Bp50, CDW40, MGC9013, TNFRSF5, p50
Description CD40 molecule, TNF receptor superfamily member 5
Transcript NM_1528    LinkOut: [ RefSeq ]
Other Transcripts NM_0012   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on CD40 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of CD40
(miRNA target sites are highlighted)
>CD40|NM_1528|3'UTR
   1 GGGCTTCGGGTCCAGCAGAAGGGCACCTCAGAAACAGACACCATCTGCACCTGTGAAGAAGGCTGGCACTGTACGAGTGA
  81 GGCCTGTGAGAGCTGTGTCCTGCACCGCTCATGCTCGCCCGGCTTTGGGGTCAAGCAGATTGCTACAGGGGTTTCTGATA
 161 CCATCTGCGAGCCCTGCCCAGTCGGCTTCTTCTCCAATGTGTCATCTGCTTTCGAAAAATGTCACCCTTGGACAAGGTCC
 241 CCAGGATCGGCTGAGAGCCCTGGTGGTGATCCCCATCATCTTCGGGATCCTGTTTGCCATCCTCTTGGTGCTGGTCTTTA
 321 TCAAAAAGGTGGCCAAGAAGCCAACCAATAAGGCCCCCCACCCCAAGCAGGAACCCCAGGAGATCAATTTTCCCGACGAT
 401 CTTCCTGGCTCCAACACTGCTGCTCCAGTGCAGGAGACTTTACATGGATGCCAACCGGTCACCCAGGAGGATGGCAAAGA
 481 GAGTCGCATCTCAGTGCAGGAGAGACAGTGAGGCTGCACCCACCCAGGAGTGTGGCCACGTGGGCAAACAGGCAGTTGGC
 561 CAGAGAGCCTGGTGCTGCTGCTGCTGTGGCGTGAGGGTGAGGGGCTGGCACTGACTGGGCATAGCTCCCCGCTTCTGCCT
 641 GCACCCCTGCAGTTTGAGACAGGAGACCTGGCACTGGATGCAGAAACAGTTCACCTTGAAGAACCTCTCACTTCACCCTG
 721 GAGCCCATCCAGTCTCCCAACTTGTATTAAAGACAGAGGCAGAAGTTTGGTGGTGGTGGTGTTGGGGTATGGTTTAGTAA
 801 TATCCACCAGACCTTCCGATCCAGCAGTTTGGTGCCCAGAGAGGCATCATGGTGGCTTCCCTGCGCCCAGGAAGCCATAT
 881 ACACAGATGCCCATTGCAGCATTGTTTGTGATAGTGAACAACTGGAAGCTGCTTAACTGTCCATCAGCAGGAGACTGGCT
 961 AAATAAAATTAGAATATATTTATACAACAGAATCTCAAAAACACTGTTGAGTAAGGAAAAAAAGGCATGCTGCTGAATGA
1041 TGGGTATGGAACTTTTTAAAAAAGTACATGCTTTTATGTATGTATATTGCCTATGGATATATGTATAAATACAATATGCA
1121 TCATATATTGATATAACAAGGGTTCTGGAAGGGTACACAGAAAACCCACAGCTCGAAGAGTGGTGACGTCTGGGGTGGGG
1201 AAGAAGGGTCTGGGGG
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' uugCCUUGGUGAUCACUGAAc 5'
             || |:|| |:|||:||| 
Target 5' agaGGCATCA-TGGTGGCTTc 3'
840 - 859 145.00 -14.10
2
miRNA  3' uugcCUUGGUGAUCACUGAAc 5'
              |||  | |:||||| | 
Target 5' gctcGAA-GAGTGGTGACGTc 3'
1171 - 1190 120.00 -7.42
3
miRNA  3' uugccuUGGUGAUCACUGAAc 5'
                | ::| :| ||||| 
Target 5' tgctccAGTGCAGGAGACTTt 3'
421 - 441 115.00 -7.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-224-5p :: CD40    [ Functional MTI ]
Validation Method Microarray , qRT-PCR , Other
Conditions A818 , ASPC1 , BxPc3 , Capan-1 , Capan-2 , HPAF-2 , MiaPaCa-2 , MPanc 96 , PANC-1 , PaTu 8902 , PaTu 8988 S , PaTu 8988 T , PT45 , Su.86.86 , Suit 0028 , Suit 007
Tools used in this research "Find miRNA Target Genes" , Literature survey , mirGen
Original Description (Extracted from the article) ... CD40 was detected as a relevant target gene for differentially expressed miRNAs observed in highly invasive and metastatic PDAC only PDAC cell-lines.//A significant overexpression (P < .05) of CD40-related miRNAs miR-224 and miR-486 was detected in highly invasive and metastatic PDAC, whereas CD40 mRNA expression was not significantly altered. Instead, CD40 protein expression at cell surfaces of these highly invasive and metastatic PDAC was significantly reduced (P < .01).//When we searched for CD40- and CD40L-targeting miRNAs in highly metastatic and highly invasive groups, we found miR-196b, miR-224, miR-424, miR-486, and miR-584 to be greatly upregulated. ...

- Mees, S. T. Mardin, W. A. Sielker, S. et al., 2009, Ann Surg Oncol.

Article - Mees, S. T. Mardin, W. A. Sielker, S. et al.
- Ann Surg Oncol, 2009
BACKGROUND: Genetic and epigenetic alterations during development of pancreatic ductal adenocarcinomas (PDAC) are well known. Genetic and epigenetic data were correlated with tumor biology to find specific alterations responsible for invasion and metastasis in pancreatic ductal adenocarcinomas. METHODS: A total of 16 human PDAC cell lines were used in murine orthotopic PDAC models. By means of standardized dissemination scores, local invasion and metastatic spread were assessed. mRNA and microRNA expression were studied by microarray and TaqMan low-density array. Quantitative real-time-polymerase chain reaction and flow cytometry were used for expression validation. RESULTS: CD40 was detected as a relevant target gene for differentially expressed miRNAs observed in highly invasive and metastatic PDAC only. A significant overexpression (P < .05) of CD40-related miRNAs miR-224 and miR-486 was detected in highly invasive and metastatic PDAC, whereas CD40 mRNA expression was not significantly altered. Instead, CD40 protein expression at cell surfaces of these highly invasive and metastatic PDAC was significantly reduced (P < .01). CONCLUSIONS: Epigenetic alterations with upregulated CD40-targeting miR-224 and miR-486 are related to downregulated CD40 protein expression at cell surfaces in highly invasive and metastatic PDAC. Thus, miRNA-regulated CD40 expression seems to play an important role in progression of PDAC. These data suggest a diagnostic and therapeutic potential for CD40 and/or its targeting miRNAs in PDAC.
LinkOut: [PMID: 19475450]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
19 hsa-miR-224-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000454 KLK10 kallikrein-related peptidase 10 3 1
MIRT000635 CXCR4 chemokine (C-X-C motif) receptor 4 4 1
MIRT000636 CDC42 cell division cycle 42 (GTP binding protein, 25kDa) 4 1
MIRT000769 RAB9B RAB9B, member RAS oncogene family 2 1
MIRT000770 PDGFRB platelet-derived growth factor receptor, beta polypeptide 2 1
MIRT003232 AP2M1 adaptor-related protein complex 2, mu 1 subunit 2 1
MIRT003233 NIT1 nitrilase 1 2 1
MIRT003234 FOSB FBJ murine osteosarcoma viral oncogene homolog B 2 1
MIRT003235 NCOA6 nuclear receptor coactivator 6 2 1
MIRT003393 CD40 CD40 molecule, TNF receptor superfamily member 5 3 1
MIRT003987 API5 apoptosis inhibitor 5 4 2
MIRT005755 EYA4 eyes absent homolog 4 (Drosophila) 3 1
MIRT005756 EDNRA endothelin receptor type A 3 1
MIRT006136 DIO1 deiodinase, iodothyronine, type I 3 1
MIRT006527 SMAD4 SMAD family member 4 2 1
MIRT007008 PEBP1 phosphatidylethanolamine binding protein 1 1 1
MIRT024135 KLK1 kallikrein 1 1 1
MIRT046570 ARSB arylsulfatase B 1 1
MIRT046571 ADNP2 ADNP homeobox 2 1 1