Accession ID: MIRT003549 [miRNA, hsa-miR-489 ::
PTPN11, target gene]
| pre-miRNA ID | hsa-mir-489 LinkOut: [miRBase ] |
|---|---|
| Synonyms | MIRN489, hsa-mir-489, MIR489 |
| Description | Homo sapiens miR-489 stem-loop |
| Comment | The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations. |
| 2nd Structure of pre-miRNA |  |
| Mature miRNA | hsa-miR-489 |
|---|---|
| Mature Sequence | 52| GUGACAUCACAUAUACGGCAGC |73 |
| Evidence | Experimental |
| Experiments | Array-Cloned |
| Putative hsa-miR-489 Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
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| Gene Symbol | PTPN11 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Synonyms | BPTP3, CFC, MGC14433, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2 | ||||||||||||||||||||
| Description | protein tyrosine phosphatase, non-receptor type 11 | ||||||||||||||||||||
| Transcript | NM_002834 LinkOut: [ RefSeq ] | ||||||||||||||||||||
| Expression | LinkOut: [ BioGPS ] | ||||||||||||||||||||
| KEGG Pathway |
hsa04630 Jak-STAT signaling pathway - Homo sapiens (human) hsa04650 Natural killer cell mediated cytotoxicity - Homo sapiens (human) hsa04670 Leukocyte transendothelial migration - Homo sapiens (human) hsa04722 Neurotrophin signaling pathway - Homo sapiens (human) hsa04920 Adipocytokine signaling pathway - Homo sapiens (human) hsa05120 Epithelial cell signaling in Helicobacter pylori infection - Homo sapiens (human) hsa05211 Renal cell carcinoma - Homo sapiens (human) hsa05220 Chronic myeloid leukemia - Homo sapiens (human) |
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| Putative miRNA Targets on PTPN11 | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] | ||||||||||||||||||||
| 3'UTR of PTPN11 (miRNA target sites are highlighted) |
>PTPN11|NM_002834|3'UTR 1 TGAGAAAACCTGCCAAAACTTCAGCACAGAAATAGATGTGGACTTTCACCCTCTCCCTAAAAAGATCAAGAACAGACGCA 81 AGAAAGTTTATGTGAAGACAGAATTTGGATTTGGAAGGCTTGCAATGTGGTTGACTACCTTTTGATAAGCAAAATTTGAA 161 ACCATTTAAAGACCACTGTATTTTAACTCAACAATACCTGCTTCCCAATTACTCATTTCCTCAGATAAGAAGAAATCATC 241 TCTACAATGTAGACAACATTATATTTTATAGAATTTGTTTGAAATTGAGGAAGCAGTTAAATTGTGCGCTGTATTTTGCA 321 GATTATGGGGATTCAAATTCTAGTAATAGGCTTTTTTATTTTTATTTTTATACCCTTAACCAGTTTAATTTTTTTTTTCC 401 TCATTGTTGGGGATGATGAGAAGAAATGATTTGGGAAAATTAAGTAACAACGACCTAGAAAAGTGAGAACAATCTCATTT 481 ACCATCATGTATCCAGTAGTGGATAATTCATTTTGATGGCTTCTATTTTTGGCCAAATGAGAATTAAGCCAGTGCCTGAG 561 ACTGTCAGAAGTTGACCTTTGCACTGGCATTAAAGAGTCATAGAAAAAGAATCATGGATATTTATGAATTAAGGTAAGAG 641 GTGTGGCTTTTTTTTTTTTCTTTTTTCCAGCCGTTGACCAATTATAGTTCGGCTGTTGACTGAGAAGTTTGTGGTGGGAA 721 AACGTTTGCCATATTTTCTTTGCATTTGAATAATTGTCTTGTACTTAGAAAAAAGGCGTCTATGAATGACCAGTGTTTTT 801 GGTCGCCAAATGTTGCTGACAAACTTATCCCAAAACTTTAGTGGCTTAAAAAAACCTGCCCCCAACTGTTAGTCAATCTG 881 AGCTGGGCTCAGCTGGGCTGTTCTTCTGCCAGCCTGCAGGTGGCCACTCATGTGGTCAGCAGGTCGGCGGAGAGACTGGG 961 ATGGCTGGGCTTCTCTCTCTGCCTGCAGTCCTGAGTCTCTCCTTCTTCGTGTAGTCTCTTTCAGTGGCCTGGCTGGCAGG 1041 GTAGCTAGACCTCTCACATGCAGCTCAGAGCTCCCAAGAGCTCAAAAGCAGAAATGGCCAGGCCTTCTGAAAACTTAAGT 1121 CCAGAATTGTCACAGTGTCCCTTCTACTTCCCTCTATTGATGATGATGATGATGATGATGATGATGATGATGATGATGAT 1201 GATGGTTTTTTCTAATCAGAAGAAAGCTGGGGTATGCCCTCTACTTACTAAACAAGTCACAAGCCCAGCTCAGATTCAAG 1281 AAAAGGGTGTGAAGTAGAGGTGCAGTTAAGTGGGGGGCCACTAGTCTAACAGACGGTCACAACCAGTGCCATGGAAAACC 1361 AAGGATATTAGCAAAAGCAGAAGTTGCTAGTGACCTTGGGAAGCCGAAGCTGCTTACAGTAGCTGGGACAAGCTGAAAGT 1441 CAGACTAAGAAATAAAGAGAGGGCCTTCAAGAAGCTTCCTGAATGATTTCTGCTAGCCCTGAGCCTATTTTTGGAACCAG 1521 CACTTGGGGAAACTGATCTTGTGAGGATGGATGTGTTTAGGGACACAGGGCTTTTGAGAGCAGCACCACCCCACTGGGGC 1601 ATCCCCAGACTTGGGAAACGTGACTCTTTCTTAATGCCACTGGGTTTTAGTCAGGCCACAGTGAGAAGGAACAGCCCTAA 1681 CAGGCCTCCAGCCAGGTTGAATGAGCTCATTTTTGTTGTAGCCAACCAGTAAGATTTGCTAATGTTCTACATTAAGTGCC 1761 TTCTCCAAAGACATCCCTCTTTGCCTCATATGTTGAATCATCCAGTGCGGATATTTCAATGAAAATATCATTGGTTGACT 1841 TTTGTGATGGTAATAATGCTATGGCATCTTTGCCATGAAGTTGTGGCCTCCTTGGATTCTTCTGACTTTGGCTTCTGAAA 1921 GGAAGGCCTAGATCCAGCCCTGGTGGTAGTTCCTTTCTGAGGTCTCTCAGTCCCTTGAGACTTTGGGGTAGTTTGGCTGC 2001 CATTCTCACTGACAAAATGTATATCAGCCCCCACCTCCACCCCCCAATATTCCTTGAACTTTGAATTGCTTCAGAACACA 2081 GGTGTGGCCTGAAGGTATTCCCTTATTAGGGAAGTGTCACTGCTGTCTTCTAGTCAAACTTGTAAAGAAAAAGATTCCAG 2161 TTCAGTATTTGCAGCAAGAAGCTTGAATGCTGTTCTTTTTATCGCATTGTTACATCGACTCATTCTCCATTTTGCTTTGG 2241 TTTTGTCTTGACTTGACTTGACTTTGGGGGTAAAGTCTTTCACCAGCACACAAGAGTTTGATTGTACAAATATATCTTCT 2321 GCATTAACATCTCTGCCTGTTGCTTAAGATCAGTTGCTTTTATACTCAGAATGGAAATACCTGATCTTGGCTAGTTTTGT 2401 TATAAGATATTGATTTCATTTAGATTTCCCTCCACGAGGTCAGCAAACTATCATGTTCTTATGTAAACTTAGGCCAAGGC 2481 CAGAGTTATCATAGTCCCTAGGTTGCTACGGCTTATCATGTGCTTGGTAAAAGGTGATCGCAGGTTCTCAGACGAGTTTA 2561 CTTTACATGAGATGGAATCAGGCAGAGAGGCTGGGATGATGGAGAAAGCTCGAGGTGAAGTTTTAAAAAAAAAGTTGTGG 2641 AAAGGAAAGTTCCAAAGAGGTGGTTTCTGAGGAAGTCAGAGCGCCCAGGGCCAGAGCAGTCAGTAATGGGTGAATGAGGT 2721 TGTTTGGAAAGTCGGTGTGACAGACACATGGATGCCATCTACTTCTAGGTTGCTGGTGGGTATTAAATATGCACAATATT 2801 CCATAGCTCACTGAGGATTTTAAAATTATAAGCATAGGATTTTATATTTTGGGGTGAAAGAATTATCTGGCACATTAGGT 2881 ATTGGAGTTTAAAAAAAAAGCCAAATTTCACAGTCTTAATAACTTTTTTTAAAAAAAACTAAAAGGCGCTTCATGTCCAG 2961 TGTGTGGCCCTTCTGAAACTTATGGTCATCTCTCCCACTGAAACCAAGGTCTTTTCAAATGTGGCTAAATGGGGATGAGG 3041 AGACACGGGTAGGACTTTCTTGGTGTGTGTGCATTCTTTAAAGAGCCAAGTTGCTTCGGGGAAACAGCCAGGAAAATGGT 3121 CAAGATTATTTTTAGAGGTTATTTTATTGGGGATTTTAAGAACTAATAACATCTTGAGTTATTTTTAATTCAGGGGGATG 3201 TGGAAAGGTTTGCAATTGTCAAGTGTTTTGTTGTAGCTTAGTATCCATAAGGGAAACTTAGACTATAGACATAACTACAA 3281 AGCCAGTGCAGCTTTTGTTTTCTGTATGTTGTTGGGGGATCAACTTTCACACATAGCAAGCACATGGCCTCCCTGATGTC 3361 AGGATGCCTTTGTTAGGATCTGTATTTGCCCTTAATTTTGTTGAAATCTTTTTTCCTTCTTCCTCTTGAAAAGTTCCAAA 3441 ATATAGTTTATTGTATCTTTCATCACTAAAAATTTGTTCCTTTTTCACTATGGGCAGTTCACACAAGGCAAAAACTATTG 3521 AACAGTTGGTTTTAGTGTGTTGTATAACTTTGCTGTATATCAAACTAATTTTGACAAGTTTTCATCCTAAGCCTCAAATC 3601 ATGTAATTAATAATTTGCCTGTTTATTTATGACCTAATTGTGATTCTTTTATTAATAAAAGCTAATGGGAAAAGGATCCC 3681 TGATTAAGCTGATGACTAGACCTACAATTAATTTTCCTGCAGTATATGAAGTATTGTACCAGAGTATTAAAAGATATGTA 3761 ATATTTTATTGATAAATCTATCCTTTAAAAGGAATACGTTTTAGGATGTCATCATTTTGATGTGAATCATGTAAATGTTG 3841 ATAATATGCTGTTTATTATACATTTAGTGTTTCAAGAGATTCACTTAATTGCCTTTTTGCCCACGTATATTATGTAGTCT 3921 ATTTGCAACTGTTCTTAAAAAAATGACATTAAAAGAATAGTTTATGTAGAGAAACATTAGTGGATGTTAATTGTCTCCCC 4001 ACCTATATTTATGGGTGTTAGCGCAACTGCTTTGCTAGTTGCAAAGCTGTATTATCAGAGTAAAAGTGTATTTGTAAACT 4081 GTATGGGAACTAAAAATTAGGAATAAAACCATTTTCTTATATGAAAAAAAAAAAAAAAAAA Target sites Provided by authors Predicted by miRanda |
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| miRNA-target interactions (Predicted by miRanda) |
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| miRNA:Target | hsa-miR-489 :: PTPN11 [ Functional MTI ] | ||||||
|---|---|---|---|---|---|---|---|
| Validation Method | Luciferase reporter assay , Western blot | ||||||
| Location of target site | 3'UTR | ||||||
| Tools used in this research | TargetScan | ||||||
| Original Description (Extracted from the article) | ... miR-489 negatively regulates PTPN11 expression.//This study is to identify tumour-suppressive miRNAs based on clinical HSCC miRNA expression signature. We have specifically identified a tumour-suppressive miRNA (miR-489) and found its direct target (PTPN11). Disruption of this interaction may lead to the deregulation of miR-489-PTPN11 signalling in HSCC.//Luminescence intensity was significantly decreased for the vectors carrying the 3'UTR and the deletion at position 3353a€“3359, but not in the vector with the deletion at position 3803a€“3809 (Figure 3), indicating that the region between positions 3803a€“3809 contains the miR-489 target site. ... - Kikkawa, N. Hanazawa, T. Fujimura, L. et al., 2010, Br J Cancer. |
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| miRNA-target interactions (Provided by authors) |
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| Article |
miR-489 is a tumour-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (HSCC)
- Kikkawa, N.
Hanazawa, T.
Fujimura, L. et al.- Br J Cancer, 2010
Background:Hypopharyngeal squamous cell carcinoma (HSCC) is an aggressive malignancy with one of the worst prognoses among all head and neck cancers. Greater understanding of the pertinent molecular oncogenic pathways could help improve diagnosis, therapy, and prevention of this disease. The aim of this study was to identify tumour-suppressive microRNAs (miRNAs), based on miRNA expression signatures from clinical HSCC specimens, and to predict their biological target genes.Methods:Expression levels of 365 human mature miRNAs from 10 HSCC clinical samples were screened using stem-loop real-time quantitative PCR. Downregulated miRNAs were used in cell proliferation assays to identify a tumour-suppressive miRNA. Genome-wide gene expression analyses were then performed to identify the target genes of the tumour-suppressive miRNA.Results:Expression analysis identified 11 upregulated and 31 downregulated miRNAs. Gain-of-function analysis of the downregulated miRNAs revealed that miR-489 inhibited cell growth in all head and neck cancer cell lines examined. The gene PTPN11 coding for a cytoplasmic protein tyrosine phosphatase containing two Src Homology 2 domains was identified as a miR-489-targeted gene. Knockdown of PTPN11 resulted in the inhibition of cell proliferation in head and neck SCC cells.Conclusion:Identification of the tumour-suppressive miRNA miR-489 and its target, PTPN11, might provide new insights into the underlying molecular mechanisms of HSCC.British Journal of Cancer advance online publication, 10 August 2010; doi:10.1038/sj.bjc.6605811 www.bjcancer.com.
LinkOut: [PMID: 20700123]
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