Accession ID: MIRT003732 [miRNA, hsa-miR-519c-3p :: ELAVL1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-519cLinkOut: [miRBase ]
Description Homo sapiens miR-519c stem-loop
Comment The 5' arm of this precursor expresses a product related to miR-526 (previously named miR-526c here). Landgraf et al. confirm mature miRNA expression from both arms of the precursor .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-519c-3p
Mature Sequence 54| AAAGUGCAUCUUUUUAGAGGAU |75
Evidence Experimental
Experiments Array-cloned
Putative hsa-miR-519c-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol ELAVL1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms ELAV1, HUR, Hua, MelG
Description ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Transcript NM_0014    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on ELAVL1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of ELAVL1
(miRNA target sites are highlighted)
>ELAVL1|NM_0014|3'UTR
   1 CTCGCTCATGCTTTTTTTTGTACGGAATAGATAATTAAGAGTGAAGGAGTTGAAACTTTTCTTGTTAGTGTACAACTCAT
  81 TTTGCGCCAATTTTCACAAGTGTTTGTCTTTGTCTGAATGAGAAGTGAGAAGGTTTTTATACTCTGGGATGCAACCGACA
 161 TGTTCAAATGTTTGAAATCCCACAATGTTAGACCAATCTTAAGTTTCGTAAGTTATTTCCTTTAAGATATATATTAAACA
 241 GAAATCTAAGTAGAACTGCATTGACTAACCAGTCCCTCTGGATGGTGGTGAACCTGAAGCATGCTTTAACCTCTAAGACT
 321 GTCTAACACGCGTTTCATTCAATGTCTCCACAGACTGGGTAGCAAAAAAATCACCTTTTAGTTTTAGTTTTTAATCTAAA
 401 GATGTTAGACAGATGCTGAGTGTGCGTTTTCTCAACCGCTTCAACATTGTAAGCGATGTATGCTTTGGTTGACAGGAAGT
 481 TCCTTTTCCAGGCAGGTCCCGTTGCCACCTCCTGCTCACTCAGTCCCGGGCTCTGCCGAGTGGTCCTGGGAATGGCGGCG
 561 GGCCCGTCCAGCGTGGGCCACCACTGGGGCCGGGGGCCACGGGCTGCATGCTGGGCGGGCCCTCCAGAGAAGGACACAAA
 641 CGTGTTTCGTAAGCCCAGGCACCAATGGGAATGGACCAAAGAGTTTCAGGGAAACTCCAGTATATTCCAGAGTCAGATCT
 721 AAGCTCCAGGCACGCCTGAAGATGTGTTGCTACTCTGACATCCCGAGTTTCTGTCCACACATTGCATGCACAGCGCCCCA
 801 CACATTGGATACTGTTGTTCACGATAATTTCTCCCGTTTTCCAGAGCATTTAACATAGCTTGGAGGCGTAAAATGGCTCT
 881 GTATTTTAATAACACAGAAACATTTGAGCATTGTATTTCTCGCATCCCTTCTCGTGAGCGCTTAGACCTTTTTCTATTTT
 961 AGTCGGATTTTGTTTTGGAATTTTGCTTTTGTATGAACACTCAGCAGAAAAGTACTTACTTCTTGCCAGTTATCTATTAA
1041 CCAAAACCTTTGATTTGTAGTTTTAAAGATTAACCCTCAAAGTTCTCTTCATAACTGCCTTGACATTTTGGGTTGTTCTG
1121 TTCTGTTAATTTTCTTTTGCTTTTTTGTGTTTTTTGTTTGTTTTTACTTTTGCATTTAAGACCATTAAATTTGATTTTGT
1201 TTTGCTCGAATTTTGTTTTGTTTTTTATTTTACCTTTTCTTTCTTTTTGGCTAGGGAAGGTGCAGGTGGCCCAGCATTCA
1281 GGGAGGAGTCGTAAGATCTTAAGAAACCAATCCTTGCCTCAAGCAAAAGCATTTCTGAATCTGTGACGCAAGAATGTGCA
1361 GTTACAGGCTGGTGGCTTTTAAACCAGGAGCCCGGAGGAAGGGTGAAAGAGAAAGCCTGTGAAATAGGCAGGGCCAGATC
1441 ACCCAAAACTCCTCAGGACTGGGATCTGGCGTTTATAAATAACTAGTTTACAGAGAGAATCACAAACAGGATAACTTAGT
1521 ACCAGCAGTTTTTAACCTTGACGTGAGACTAAAACGTGACCGTAGGCTGTTTTTTAGTTATTGCTCTCATGAGATGATGG
1601 CTGTATTTATCTGTTTATTTATACCTATTTATGTATTTATTTATTGAAGTGTGAAATTGAGCAATAGGCAGGCACCACCG
1681 TCCCCAGAGCAGGTCAGCGTCTCGAGAGGCCCCTGGACAATTGAGGATGCCCATCCCCTCCCCTTTCCCTGATCTTTTAC
1761 TGAGGGGCTGTGTGCGCGATCCTTGCAAATTGATGATGTTGCCATCCGTACCCAGGCTGTGTCTCATAAAAGTCGGCCTG
1841 GTGCCAGAGAGGACCTCCTTTCTCCCACAGAATCCCAGATCCTCAGGAAAAGCCAAAACCGAGGCCCATTGCCCGGATTC
1921 GACACAAAAGAGGGTCCCTGCTCTGTTGCCCGAGAGCAGTCTGCATCCTGGGACCAGAATGCTTTCCTGGAAAAAGAAGC
2001 CTTTCAGGTTTCCCTGGGCCAGCATCTTCTGATGGAAGGTGGGAGCCAACACCCTTCTGATGGAAGGTGGGAGCCAACAC
2081 CCTTCTGATGGAAGGTGGGAGCCAACACCCTTCTGATGGAAGGTGGGAGCCAACACCCTTCTGATGGAAGGTGGGAGCCA
2161 ACACCCTTCTGATGGAAGGCGGGATTCCCGCTTCTGAAACTCCCCCTGGAGTCTCACTCCCACACATGCCCATAGCTAGC
2241 ATTCAACAGAGAACTCTGTCTTAAGCTTCAACTGTGAAAATGATGACGGGCTTGTAGCACCTCAGCTTCTTTCCTCGCCC
2321 CCTTTTATCTGAATCCTATCAATTATTCTGATGCTGGGACAGGTGAGAAGAAACTGTGAAGTATATGAGCCTTTGAAAGT
2401 TCCCTGAAGTTTCTCAGTTCAGGAACATTCTCATTGTATGTGGTCTCCGCTGTTTGAACAGCCTTCTAGCTAAAAAATTC
2481 CAAAGCCTTTATTTGGGAGTCTTAGCTTGCAAGCTTGTGGAAGGATTTAGCTTAACAACTGTCACTCCTGAAAAGCAATC
2561 TCTGTTCCATCAAGGTTCTAGTTGCTGGCCCTGTGTCCTCAAAGTTCATTACATCTTATCAAGGCCTGTTTGCAAAGGGG
2641 AGATCCCTTTTCTTAAAAAAGGCTCAACCCAAAAGAAACCATTTCTTAAAAAATTTTACATAGATCAGTTGTATTTCTAT
2721 TTAGCAAAAATGAGTGCTCTGCTTTTATTTGGGAATTTCGATGAAAAAGCGTTCAGAGTAGATAATGTTCATTTATCAAA
2801 AATCTGGTTTGGGAAATACCAAAGAGGCTTTGATTGAATTCCCTTTTGACCCGTGTGTAACTTCCTCTGGTAGTTAGACC
2881 CCAGGCAGCTCCGAATTTGTGAACCTGCTTCCTGATGAATTCTCCCTTGTTCCCCCTTGGCTCTGCCATTATTTCGTTTT
2961 CAGTGTAATTTGCCAAGCCGCAGTTTTCTGTGCTGGCTGTGCCTCTAGTCGCAGCTCTGTGACTGATTCCCTCCCGGGTG
3041 CTGAGTCCCCTCCCCGGCCACCATCCTGCGTGAATATCCTGAAATTCATGGGCTTCCTCGGGGGCCCGCCCGCAGGTGGT
3121 GCTGGGTGGGTTCCGCCACCTTCTCCTGGAAGGTGAGCCTTTTCCTGGCCAAGGGCAGCTGCCTTAACCTCTGAGAGTCT
3201 GCGCTTGGCCTTAGTCCTGGAGACCCAGCCTCCAGGGACTGAACCGTGCTGCTGTTGGGAGCCAAGACCGGCCCTTTGGA
3281 GCCGGCAGCCCAGGGGTCCCTGCTGGATCAGAGAAATAGAAGCACCCGAAGACGGTTAGTGGCAATTCCTTGACCCGGTT
3361 TGCTTCCAAATGAAGGCCATTTGTCCACCAGGCATTGAAAAGACATGACTTACCCAGTCCGGCATCGGACTTGAAAAATC
3441 GAAATTGACATCACTCAGCTGTTACATTTCACATCCGATTCAGCCCCCTTTTATTTCCATGTGCTTTTCGCAGCCTTCCT
3521 GTGTTGGATGAAAGAGAATAAGAATTCAGCTGACAGGAGGCCTCTATCCTGTCCCTCCACCCCACCCTCCACCTCAATCC
3601 CCTCCCATCTTCCCCAGACCTACCTCACCTACTAGGACCTGAGGCAGCTCCTTAGCAGAGACCCCTGGGGTTTAGCTGAC
3681 TCTGGGGGTCAGGGGTTCCTGTCCCCAAACTTCGCAAGACAGCCCTGAAGTCACAAGTGCTTTCTTTTAAGTGGCATTGG
3761 CAATTGGCGTGTAATGATGGCAGTAGAATCTGAATCTCGGATCCCAGGCAGGGTTCACATTTCCAAACCTTTTTGATTTC
3841 CCCTGACCTCTAATGGCTGGATCCTATTTTTCTACAACCTTTCAGTGACATCGTTCAGGTTTCTTTCTTGGCCATTTAAA
3921 AAAACAAATTTTTTTTTTCTCACTTGTAAGTCACCGCCAGTACCTAAGTTAGGCTAACGGAGACTTTGACAGGACTGGAT
4001 TTTTCTTCCACCAGAAGAGAAGCCTTTTCCGTTGGTTTGGGGCCACCTCTTTGACCATGACCATGTGATGTTCCGTTTAC
4081 AGTGACTTGCTTTGGGGGAGGGGAGGCTCTCTTAACCGATTCCCATGTTGTACAGTAGATGGTTAGACCTTTTGTATATT
4161 AGTGTGTTTTAAGTTATTGATTTGTTTTATATAAAATAATTTATTTTTCAGGTGCCATTTTTCATTTTAACTTTGTTTTT
4241 ACATGGGTTTGTTTTCAATAAAGTCTGACACTGGTGTCCAAAAGTCAACAATAAAATGAATCCCATTGTGTTCTTTTGAA
4321 GATGCCTATGTAACTTTTAAGCTTTTTAAATTATTTTCAGAAAAAAAAAAAGAAAAGCCCTTATCAGTTTTCCATCAGCC
4401 CATTGCCTTTTTATTTTTTTTTTTTAATCCTTGTGAATAAATGTTCTTTAGTGTTTTAGGAGGAAAAAGCAAACCTAGAT
4481 TTTGATAACCCAGAAGACTTCAGATTAATAAAGAAGCTTTGAAAGAAGACCATTTTTCAAAATTTTAGTGAAGTGTGAAT
4561 ATTTTTTGTCAATGGCTTTCTCAAAGAGAATGAAACTTTTGCACCATTTTCAGAGTTTTTATAGAGATGCCAAATTGATA
4641 TATTTACATGTAATGGAAACATGAAAAAGTTTTATTAAACAATTGTTCATAGCTGTGTAGACATTTTAATTCAGTTTCCA
4721 AAGCTCTCAAAAAATCGTATTTTTGAAGTACGGAGTGATGCGGTTTGGGGCGTGGCTTACAGTTCCAACGACTCAATTGT
4801 CCCGATACTCAGTTCTTTCTACAGGTATCAGGTTCGTGTTAAACGCTGTATGTTAACTATGACTGGAATTCTGTGATATT
4881 TTGGTAATAAATGAAGTGGGATCATTGCGA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' uaGGAGAUUUUUCUACGUGAAa 5'
            |||||:|:|:  |||:||| 
Target 5' aaCCTCTGAGAGTCTGCGCTTg 3'
3186 - 3207 156.00 -20.80
2
miRNA  3' uaggAGAUUUUUCU--ACGUGAaa 5'
              |||||: |||  ||||:|  
Target 5' gaaaTCTAAGTAGAACTGCATTga 3'
241 - 264 132.00 -10.20
3
miRNA  3' uaggagaUUUUUCUACGUGAAa 5'
                 ||:|| :|||| || 
Target 5' gtgacgcAAGAATGTGCAGTTa 3'
1343 - 1364 127.00 -6.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-519c-3p :: ELAVL1    [ Functional MTI ]
Validation Method qRT-PCR , Western blot ,
Conditions HeLa , HCT116 , RKO
Location of target site CDS, 3'UTR
Tools used in this research RNA22
Original Description (Extracted from the article) ... The influence of miR-519 was recapitulated using heterologous reporter constructs that revealed a greater repressive effect on the HuR CR than the HuR 3-UTR target sequences. miR-519 did not alter HuR mRNA abundance, but reduced HuR biosynthesis, as determined by measuring nascent HuR translation and HuR mRNA association with polysomes. We describe the repression of HuR expression by miR-519. Our findings indicate that miR-519 reduces HuR translation and not HuR mRNA levels or stability, since miR-519 overexpression specifically lowered HuR nascent translation and miR-519 inhibi- tion elevated it. ...

- Abdelmohsen, K. Srikantan, S. Kuwano, Y. et al., 2008, Proc Natl Acad Sci U S A.

Article - Abdelmohsen, K. Srikantan, S. Kuwano, Y. et al.
- Proc Natl Acad Sci U S A, 2008
Gene expression is potently regulated through the action of RNA-binding proteins (RBPs) and microRNAs (miRNAs). Here, we present evidence of a miRNA regulating an RBP. The RBP HuR can stabilize and modulate the translation of numerous target mRNAs involved in cell proliferation, but little is known about the mechanisms that regulate HuR abundance. We identified two putative sites of miR-519 interaction on the HuR mRNA, one in its coding region (CR), one in its 3'-untranslated region (UTR). In several human carcinoma cell lines tested, HeLa (cervical), HCT116 and RKO (colon), and A2780 (ovarian), overexpression of a miR-519 precursor [(Pre)miR-519] reduced HuR abundance, while inhibiting miR-519 by using an antisense RNA [(AS)miR-519] elevated HuR levels. The influence of miR-519 was recapitulated using heterologous reporter constructs that revealed a greater repressive effect on the HuR CR than the HuR 3'-UTR target sequences. miR-519 did not alter HuR mRNA abundance, but reduced HuR biosynthesis, as determined by measuring nascent HuR translation and HuR mRNA association with polysomes. Modulation of miR-519 leading to altered HuR levels in turn affected the levels of proteins encoded by HuR target mRNAs. In keeping with HuR's proliferative influence, (AS)miR-519 significantly increased cell number and [(3)H]-thymidine incorporation, while (Pre)miR-519 reduced these parameters. Importantly, the growth-promoting effects of (AS)miR-519 required the presence of HuR, because downregulation of HuR by RNAi dramatically suppressed its proliferative action. In sum, miR-519 represses HuR translation, in turn reducing HuR-regulated gene expression and cell division.
LinkOut: [PMID: 19088191]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
6 hsa-miR-519c-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000496 HIF1A hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) 4 1
MIRT000998 ABCG2 ATP-binding cassette, sub-family G (WHITE), member 2 4 3
MIRT003732 ELAVL1 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) 3 1
MIRT006639 TIMP2 TIMP metallopeptidase inhibitor 2 3 1
MIRT006640 PTEN phosphatase and tensin homolog 3 1
MIRT006641 CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) 3 1