Accession ID: MIRT003765 [miRNA, hsa-miR-1 ::
PNP, target gene]
| pre-miRNA ID | hsa-mir-1-2 LinkOut: [miRBase ] |
|---|---|
| Synonyms | MIRN1-2, hsa-mir-1-2, miRNA1-2, MIR1-2 |
| Description | Homo sapiens miR-1-2 stem-loop |
| Comment | Lagos-Quintana et al. . |
| 2nd Structure of pre-miRNA |  |
| Mature miRNA | hsa-miR-1 |
|---|---|
| Mature Sequence | 53| UGGAAUGUAAAGAAGUAUGUAU |74 |
| Evidence | Experimental |
| Experiments | Cloned |
| Expression Profile |  |
| Putative hsa-miR-1 Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
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| Gene Symbol | PNP LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Synonyms | FLJ94043, FLJ97288, FLJ97312, MGC117396, MGC125915, MGC125916, NP, PRO1837, PUNP | ||||||||||||||||||||
| Description | purine nucleoside phosphorylase | ||||||||||||||||||||
| Transcript | NM_000270 LinkOut: [ RefSeq ] | ||||||||||||||||||||
| Expression | LinkOut: [ BioGPS ] | ||||||||||||||||||||
| KEGG Pathway |
hsa00230 Purine metabolism - Homo sapiens (human) hsa00240 Pyrimidine metabolism - Homo sapiens (human) hsa00760 Nicotinate and nicotinamide metabolism - Homo sapiens (human) hsa01100 Metabolic pathways - Homo sapiens (human) |
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| Putative miRNA Targets on PNP | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] | ||||||||||||||||||||
| 3'UTR of PNP (miRNA target sites are highlighted) |
>PNP|NM_000270|3'UTR 1 TGACCTGCCTTGGAGTCGTCTGGCATCTCCCACACAAGACCCAAGTAGCTGCTACCTTCTTTGGCCCCTTGCTGGAGTCA 81 TGTGCCTCTGTCCTTAGGTTGTAGCAGAAAGGAAAAGATTCCTGTCCTTCACCTTTCCCACTTTCTTCTACCAGACCCTT 161 CTGGTGCCAGATCCTCTTCTCAAAGCTGGGATTACAGGTGTGAGCATAGTGAGACCTTGGCGCTACAAAATAAAGCTGTT 241 CTCATTCCTGTTCTTTCTTACACAAGAGCTGGAGCCCGTGCCCTACCACACATCTGTGGAGATGCCCAGGATTTGACTCG 321 GGCCTTAGAACTTTGCATAGCAGCTGCTACTAGCTCTTTGAGATAATACATTCCGAGGGGCTCAGTTCTGCCTTATCTAA 401 ATCACCAGAGACCAAACAAGGACTAATCCAATACCTCTTGGATTTTATTTAATGTCATAATGTTGTCAGAATAAAGAGAA 481 AGATGAAATAATTTCATTTTTTTGTGTAACTTGGTATGGGGCTGGGGCACAGACCAAGATTGACATGAAAGGATGTGAGA 561 TCGCATGTCTTGTGTGACTATCTGCTTCTCAGACAAGCAGTTAGGAACTGAGATGAGATAGTATGTGAGGGCAGCAAAGG 641 ATGAAGAAGGGCAAAATGATGAAAGGTGAGGTGGAAAGAGGTTATGAGATGGTAAAGAAAAGTTAACTTCTGGCACTTGA 721 TTGCCACTTCTGTCAGGCTGGTCCTGCCTCTCTCCCTTGCCTTCTGATTGTTTCATTTCCTGTTTATTTGATCATATCTG 801 AATTAGTTCACTGGTTAGCCTCTTCCTTAGTTCCCACTTCCTTACCAAAGCCCTAATTATATTTCCTCTTGTTTGCCTTT 881 TCTCTCCTACTCTTCTCTAACATCTGCAGCCACACTCTCCATTCACTCCATGCTGACAAGGCAGTGGCAAACACTTTTCT 961 CTGCTGCCAGCCACTCCACTGTTGACTGGATTGCTGCCAGCCCCAGGCAAACCTGTGAAGTTGTTTCATACTCTGCTTCT 1041 CTTTGAGTGCCTCCTTCTCTCCTTCCTCTTCCTTTCTGGGCTCCAGTCTTTCTCTTCACTTGTGCTTGTCAGAACCTCCC 1121 TGTGATACTGCCTCCAGGCATTTCCCCCATGTTGGCTCACCGCACTATTATCTTTGCTTATCAACTTGCATTCAGCTGGC 1201 TGGCATGTTTCAAAACCACACTGCCCTCCCAGGCCTGTGTGCCTTTTGAGAAAGACCAGTGCTGGATGAGCCTCTAGTAA 1281 TGACAACATTTTAGTTGTTAGTGGTATAATACGGAAGAGATATTTTGCACAGGCTGCTTTGGAGAACTTTCAAATTATCC 1361 TTTGTTTGGTAACTGACCTACTTAACTGCCCAATACAAAGAAAAAGCAAAAAAAAAAAAAAAAAA Target sites Provided by authors Predicted by miRanda |
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| miRNA-target interactions (Predicted by miRanda) |
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| miRNA:Target | hsa-miR-1 :: PNP [ Functional MTI ] |
|---|---|
| Validation Method | Microarray |
| Conditions | HeLa |
| Location of target site | 3'UTR |
| Original Description (Extracted from the article) | ... Filtering the expression profiles for genes characterized by the LocusLink database12 that were significantly downregulated (P < 0.001) at both 12 and 24 h, gave sets of 96 and 174 annotated genes downregulated by miR-1 and miR-124, respectively (Supplementary Tables 1 and 2).//{These MTI shown in Supplementary Table 1,2,4} ... - Lim, L. P. Lau, N. C. Garrett-Engele, P. et al., 2005, Nature. |
| Article |
- Lim, L. P.
Lau, N. C.
Garrett-Engele, P. et al. - Nature, 2005
MicroRNAs (miRNAs) are a class of noncoding RNAs that post-transcriptionally regulate gene expression in plants and animals. To investigate the influence of miRNAs on transcript levels, we transfected miRNAs into human cells and used microarrays to examine changes in the messenger RNA profile. Here we show that delivering miR-124 causes the expression profile to shift towards that of brain, the organ in which miR-124 is preferentially expressed, whereas delivering miR-1 shifts the profile towards that of muscle, where miR-1 is preferentially expressed. In each case, about 100 messages were downregulated after 12 h. The 3' untranslated regions of these messages had a significant propensity to pair to the 5' region of the miRNA, as expected if many of these messages are the direct targets of the miRNAs. Our results suggest that metazoan miRNAs can reduce the levels of many of their target transcripts, not just the amount of protein deriving from these transcripts. Moreover, miR-1 and miR-124, and presumably other tissue-specific miRNAs, seem to downregulate a far greater number of targets than previously appreciated, thereby helping to define tissue-specific gene expression in humans.
LinkOut: [PMID: 15685193]
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| miRNA:Target | hsa-miR-1 :: PNP [ Functional MTI ] | ||||||
|---|---|---|---|---|---|---|---|
| Validation Method | Luciferase reporter assay , Western blot | ||||||
| Conditions | PC3, DU145 | ||||||
| Location of target site | 3'UTR | ||||||
| Tools used in this research | miRBase Target Database, TargetScan | ||||||
| Original Description (Extracted from the article) | ... PNP as a target of post-transcriptional repression by miR-1 and miR-133a ... - Kojima, S. Chiyomaru, T. Kawakami, K. et al., 2012, Br J Cancer. |
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| miRNA-target interactions (Provided by authors) |
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| Article |
- Kojima, S.
Chiyomaru, T.
Kawakami, K. et al. - Br J Cancer, 2012
Background:Our recent analyses of miRNA expression signatures showed that miR-1 and miR-133a were significantly reduced in several types of cancer. Interestingly, miR-1 and miR-133a are located on the same chromosomal locus in the human genome. We examined the functional significance of miR-1 and miR-133a in prostate cancer (PCa) cells and identified the novel molecular targets regulated by both miR-1 and miR-133a.Methods and Results:The expression levels of miR-1 and miR-133a were significantly downregulated in PCa compared with non-PCa tissues. Restoration of miR-1 or miR-133a in PC3 and DU145 cells revealed significant inhibition of proliferation, migration, and invasion. Molecular target identification by genome-wide gene expression analysis and luciferase reporter assay showed that purine nucleoside phosphorylase (PNP) was directly regulated by both miRNAs. Silencing of the PNP gene inhibited proliferation, migration, and invasion in both PC3 and DU145 cells. Immunohistochemistry detected positive staining of PNP in PCa specimens.Conclusions:Downregulation of miR-1 and miR-133a was a frequent event in PCa and both function as tumour suppressors. The PNP is a novel target gene of both miRNAs and potentially functions as an oncogene. Therefore, identification of novel molecular networks regulated by miRNAs may provide new insights into the underlying causes of PCa oncogenesis.
LinkOut: [PMID: 22068816]
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