Accession ID: MIRT003856 [miRNA, hsa-miR-23a ::
POU4F2, target gene]
| pre-miRNA ID | hsa-mir-23a LinkOut: [miRBase ] |
|---|---|
| Synonyms | MIRN23A, hsa-mir-23a, miRNA23A, MIR23A |
| Description | Homo sapiens miR-23a stem-loop |
| Comment | This miRNA was previously named miR-23 . This finding was later retracted after the discovery that the regulated gene was human homolog of ES1 (HES1), whose function is unknown. |
| 2nd Structure of pre-miRNA |  |
| Mature miRNA | hsa-miR-23a-3p |
|---|---|
| Mature Sequence | 45| AUCACAUUGCCAGGGAUUUCC |65 |
| Evidence | Experimental |
| Experiments | Cloned |
| Putative hsa-miR-23a-3p Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
| Mature miRNA | hsa-miR-23a-5p |
| Mature Sequence | 9| GGGGUUCCUGGGGAUGGGAUUU |30 |
| Evidence | Experimental |
| Experiments | Cloned |
| Putative hsa-miR-23a-5p Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
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| Gene Symbol | POU4F2 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Synonyms | BRN3.2, BRN3B, Brn-3b | ||||||||||||||||||||
| Description | POU class 4 homeobox 2 | ||||||||||||||||||||
| Transcript | NM_004575 LinkOut: [ RefSeq ] | ||||||||||||||||||||
| Expression | LinkOut: [ BioGPS ] | ||||||||||||||||||||
| Putative miRNA Targets on POU4F2 | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] | ||||||||||||||||||||
| 3'UTR of POU4F2 (miRNA target sites are highlighted) |
>POU4F2|NM_004575|3'UTR 1 TAGAAGACTCTTGGCCTCTCCAGAGACGCCCCTTTCCTCGTCCGCTCTTTTCTCTCCTCTCTTCTGCCTCTTTTCACTTT 81 TGGCGACTAGAAACAATTCCAGTAAATGTGAATCTCGACAAATCGAGGACTGAAGAGGGAGCGAACGAGCGAACAACTGA 161 GCCCAAGCCGGTGAGAATGTGAAACAGTTTCTCAAAGGAAAGAATAACAAAAGATGGTATTTGTCTGTTGTAGCAAAGTT 241 GTCCCTTTGAACCCCACCTCGGCTTCTTCAGAGGAAGTGTGGAGATGGCTGTTTGCAGGAAGGCAGACGAGACAGTGTTT 321 AAAAAGTCCACAAGAATGATCAAGTAAGATTTGTTTTTATTCTTACAGACATCACCCGTGTTCAAGTTTAAAAGTACACT 401 TTGCAACTATTTTTCAGAAATAGAAATTGATTCAGGACTAAAACTTTAAACTAGAGTTGATGCTTAATGTGATAGAGACA 481 TCTCTAAAGTATTTTGAATTTTAAAAAAAGATGGCAGATTTTCTGCATTTACACTGTATATTATATATATATTTTTATTG 561 TGGTTCTTACCCCCTTTTCCTTCTCTGAAGTGTTAATGCTTAAGAAAAGAGTTGCGCCTGCTGTGTTCACTGATCTTGAA 641 AGCTATTATTAGATTATTGCAGAACAACCCTCTGTAAATTATTAATTTATCTCTCTAGCAACTTAATTTTGTGCACATTC 721 TAATTAATTAAACTTCTTCCGTCTAAAAAAAGTGGGGGAAATGTATAGCTAGTAACGTTCAAAAAATTTTGTTTGATGAG 801 TTTACCGAATTTTTACAGCTTTCCTCCTATACTGTGTTCCTTTTGACCCATTTGTATATTCTCACTTGAATGAAGATTGT 881 TTTTTTCTTTGTTTTTACTGGTAGTGTTCTGATTTGTGAGTCGACACTCAGTAATGGATGTCTTAATCGTGTAGACCTGA 961 TTCACTGTCTGAAGTATTGTTTACTTCGTTACATATTTAATGGGGATTCCCACATTGTCCCCATGACACATGAGCGCTCT 1041 CACTTACCCTTACACACACACACACACACACACACACCTCTAACAGAAGGGAAGAAGCAGTTGGAAGCATGACCGATGCA 1121 CCATTTTCTAGTTTTAGGTGCATTTGCCACTTGGTGTTTGCCCTTCAGATTTTAGATTTCACCAAGGTATTTCAGTCTTC 1201 CAGTTTTCAATTGCTTTGTTGGCTACATGTTAATATTTATAGGAATACTTCAGTTTTTCCTTTTGGAGGTTTGTTTGTAG 1281 AAAAACTAATTTGAACTATAAGAAAGACAGTGCACTGCTTGTAAATTCACATTGTTTGGAAAAATTCTTTTGGAACAAAA 1361 AATTAGGTACATGATAACTGGTACCTTATCTACTGTAAATATTTCATTAAAAATGATGCACACATAGATATATTCTTACA 1441 AATTTTGCTGTATTGCTGTTCTCTTTGAGGCTCTCCAAAGTCTTGAGTTCTGTATATGGCCTGGTTTCTTGTTTTTATTA 1521 ATAGATGGTTTATTTACTATGGTAATGTATTAATTTATTTTTGGTGTTGTTCGATTGTCTTTCATTGAAGAGATAATTTT 1601 AATGTTTTATTGGCAACGTATGCTGCTTTTTCATTAAAATATGCTATTAAAATTAAATGGCTTTTA Target sites Provided by authors Predicted by miRanda |
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| miRNA-target interactions (Predicted by miRanda) |
|
| miRNA:Target | hsa-miR-23a :: POU4F2 [ Functional MTI ] |
|---|---|
| Validation Method | Luciferase reporter assay |
| Conditions | HeLa |
| Location of target site | 3'UTR |
| Original Description (Extracted from the article) | ... Experimental Support for Predicted Targets.//{These MTI shown in Fig. 3} ... - Lewis, B. P. Shih, I. H. Jones-Rhoades, M. et al., 2003, Cell. |
| Article |
- Lewis, B. P.
Shih, I. H.
Jones-Rhoades, M. et al. - Cell, 2003
MicroRNAs (miRNAs) can play important gene regulatory roles in nematodes, insects, and plants by basepairing to mRNAs to specify posttranscriptional repression of these messages. However, the mRNAs regulated by vertebrate miRNAs are all unknown. Here we predict more than 400 regulatory target genes for the conserved vertebrate miRNAs by identifying mRNAs with conserved pairing to the 5' region of the miRNA and evaluating the number and quality of these complementary sites. Rigorous tests using shuffled miRNA controls supported a majority of these predictions, with the fraction of false positives estimated at 31% for targets identified in human, mouse, and rat and 22% for targets identified in pufferfish as well as mammals. Eleven predicted targets (out of 15 tested) were supported experimentally using a HeLa cell reporter system. The predicted regulatory targets of mammalian miRNAs were enriched for genes involved in transcriptional regulation but also encompassed an unexpectedly broad range of other functions.
LinkOut: [PMID: 14697198]
|
| miRNA:Target | hsa-miR-23a :: POU4F2 [ Functional MTI ] |
|---|---|
| Validation Method | Flow , GFP reporter assay , Northern blot , qRT-PCR , Western blot |
| Conditions | RGC-5, MCF-7, ND-7 |
| Location of target site | 3'UTR |
| Original Description (Extracted from the article) | ... As shown in Fig. 3A, GFP levels are reduced in RGC-5 cells only upon the co-transfection of mir-23 and mir-214. In contrast, the GFP::3b-3'UTR construct is down-regulated by mir-23, mir-214 or a combination of both in the ND7 neuroblastoma cell line.// ... - Calissano, M. Latchman, D. S., 2010, Mol Cell Neurosci. |
| Article |
- Calissano, M.
Latchman, D. S. - Mol Cell Neurosci, 2010
We have previously shown that the Brn-3b transcription factor is subjected to post-transcriptional gene regulation by specific microRNAs (mir-23 and mir-214) in the ND7 and SHSY-5Y neuronal cell lines (Calissano et al., 2007). As Brn-3b plays an essential role in the survival of retinal ganglion cells in the rat (Erkman et al., 1996; Gan et al., 1996; Gan et al., 1999; Erkman et al., 2000), we wanted to investigate whether mir-23 and mir-214 are expressed and target Brn-3b mRNA in a retinal ganglion cell line (RGC-5) thus potentially killing the cells expressing it. Here we show that, possibly due to its pro-survival role, Brn-3b is protected from degradation by microRNAs in RGC-5 cells in contrast to its fate in other cell types. This seems to be accomplished by i) the lack of expression of one of the two microRNAs targeting its 3'UTR and by ii) the requirement of at least two distinct microRNAs to mediate its down-regulation in retinal ganglion cells. We speculate that this mechanism could have a widespread role in the regulation of mRNAs encoding for essential proteins.
LinkOut: [PMID: 20609388]
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