Accession ID: MIRT003918 [miRNA, hsa-miR-373-3p :: XPA, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-373LinkOut: [miRBase ]
Synonyms MIRN373, hsa-mir-373, miRNA373, MIR373
Description Homo sapiens miR-373 stem-loop
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-373-3p
Mature Sequence 44| GAAGUGCUUCGAUUUUGGGGUGU |66
Evidence Experimental
Experiments Cloned
Putative hsa-miR-373-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol XPA LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms XP1, XPAC
Description xeroderma pigmentosum, complementation group A
Transcript NM_0003    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on XPA LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of XPA
(miRNA target sites are highlighted)
>XPA|NM_0003|3'UTR
   1 TGATTTTTTAGTTCAGTGACCTGTTTTATAGAATTTTATATTTAAATAAAGGAAATTTAGATTGGTCCTTTTCAAAATTC
  81 AAAAAAAAAAGCAACATCTTCATAGATGAATGAAACCCTTGTATAAGTAATACTTCAGTAATAATTATGTATGTTATGGC
 161 TTAAAAGCAAGTTTCAGTGAAGGTCACCTGGCCTGGTTGTGTGCACAATGTCATGTCTGTGATTGCCTTCTTACAACAGA
 241 GATGGGAGCTGAGTGCTAGAGTAGGTGCAGAAGTGGTAGGTCAGCTACAAATTTGAGGACAAGATACCAAGGCAAACCCT
 321 AGATTGGGGTAGAGGGAAAAGGGTTCAACAAAGGCTGAACTGGATTCTTAACCAAGAAACAAATAATAGCAATGGTGGTG
 401 CACCACTGTACCCCAGGTTCTAGTCATGTGTTTTTTAGGACGATTTCTGTCTCCACGATGGTGGAAACAGTGGGGAACTA
 481 CTGCTGGAAAAAGCCCTAATAGCAGAAATAAACATTGAGTTGTACGAGTCTGAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-373-3p :: XPA    [ Functional MTI ]
Validation Method Western blot , Other
Conditions HeLa , MCF-7
Original Description (Extracted from the article) ... In addition, the expression of two other repair factors, XPA (involved in NER) and MRE11 (involved in HDR), was not altered by miR-210 or miR-373 (Supplementary Fig. S1B).//Forced expression of miR-210 and miR-373 specifically regulates RAD52 expression, but does not regulate MRE11 or XPA expression. ...

- Crosby, M. E. Kulshreshtha, R. Ivan, M. et al., 2009, Cancer Res.

Article - Crosby, M. E. Kulshreshtha, R. Ivan, M. et al.
- Cancer Res, 2009
Genetic instability is a hallmark of cancer; the hypoxic tumor microenvironment has been implicated as a cause of this phenomenon. MicroRNAs (miR) are small nonprotein coding RNAs that can regulate various cellular pathways. We report here that two miRs, miR-210 and miR-373, are up-regulated in a hypoxia-inducible factor-1alpha-dependent manner in hypoxic cells. Bioinformatics analyses suggested that these miRs could regulate factors implicated in DNA repair pathways. Forced expression of miR-210 was found to suppress the levels of RAD52, which is a key factor in homology-dependent repair (HDR); the forced expression of miR-373 led to a reduction in the nucleotide excision repair (NER) protein, RAD23B, as well as in RAD52. Consistent with these results, both RAD52 and RAD23B were found to be down-regulated in hypoxia, but in both cases, the hypoxia-induced down-regulation could be partially reversed by antisense inhibition of miR-210 and miR-373. Importantly, luciferase reporter assays indicated that miR-210 is capable of interacting with the 3' untranslated region (UTR) of RAD52 and that miR-373 can act on the 3' UTR of RAD23B. These results indicate that hypoxia-inducible miR-210 and miR-373 play roles in modulating the expression levels of key proteins involved in the HDR and NER pathways, providing new mechanistic insight into the effect of hypoxia on DNA repair and genetic instability in cancer.
LinkOut: [PMID: 19141645]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
82 hsa-miR-373-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000056 RAD23B RAD23 homolog B (S. cerevisiae) 4 2
MIRT000606 RECK reversion-inducing-cysteine-rich protein with kazal motifs 4 1
MIRT000721 VEGFA vascular endothelial growth factor A 2 2
MIRT001754 LATS2 LATS, large tumor suppressor, homolog 2 (Drosophila) 5 5
MIRT001931 CSDC2 cold shock domain containing C2, RNA binding 2 1
MIRT002428 CD44 CD44 molecule (Indian blood group) 4 4
MIRT002495 KIF23 kinesin family member 23 2 1
MIRT002496 GBAS glioblastoma amplified sequence 2 1
MIRT002498 C2orf18 chromosome 2 open reading frame 18 2 1
MIRT002500 PHC2 polyhomeotic homolog 2 (Drosophila) 2 1
MIRT002501 CD24 CD24 molecule 2 1
MIRT002504 NUPL1 nucleoporin like 1 2 1
MIRT002505 MYBL1 v-myb myeloblastosis viral oncogene homolog (avian)-like 1 1 1
MIRT002506 KLHL12 kelch-like 12 (Drosophila) 2 1
MIRT002508 HERPUD1 homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1 2 1
MIRT002509 LUC7L2 LUC7-like 2 (S. cerevisiae) 2 1
MIRT002510 LMNB1 lamin B1 2 1
MIRT002511 MKRN1 makorin ring finger protein 1 2 1
MIRT002512 ARHGEF3 Rho guanine nucleotide exchange factor (GEF) 3 2 1
MIRT002515 FYCO1 FYVE and coiled-coil domain containing 1 2 1
MIRT002516 CD83 CD83 molecule 2 1
MIRT002517 GBP3 guanylate binding protein 3 2 1
MIRT002520 RNF149 ring finger protein 149 2 1
MIRT002521 TNFAIP1 tumor necrosis factor, alpha-induced protein 1 (endothelial) 2 1
MIRT002522 STX11 syntaxin 11 2 1
MIRT002523 FITM2 fat storage-inducing transmembrane protein 2 2 1
MIRT002524 GPSM2 G-protein signaling modulator 2 (AGS3-like, C. elegans) 2 1
MIRT002525 CENPF centromere protein F, 350/400ka (mitosin) 2 1
MIRT002526 CFL2 cofilin 2 (muscle) 2 1
MIRT002528 TUSC2 tumor suppressor candidate 2 2 1
MIRT002529 INSIG2 insulin induced gene 2 2 1
MIRT002530 GLTP glycolipid transfer protein 2 1
MIRT002531 RELA v-rel reticuloendotheliosis viral oncogene homolog A (avian) 2 1
MIRT002532 PRC1 protein regulator of cytokinesis 1 2 1
MIRT002533 BAZ1A bromodomain adjacent to zinc finger domain, 1A 2 1
MIRT002534 USP12 ubiquitin specific peptidase 12 2 1
MIRT002535 NIN ninein (GSK3B interacting protein) 2 1
MIRT002537 TBC1D2 TBC1 domain family, member 2 2 1
MIRT002538 TFAP4 transcription factor AP-4 (activating enhancer binding protein 4) 2 1
MIRT002540 TMEM14A transmembrane protein 14A 2 1
MIRT002541 STK4 serine/threonine kinase 4 2 1
MIRT002542 ZNF226 zinc finger protein 226 2 1
MIRT002543 HSPA14 heat shock 70kDa protein 14 2 1
MIRT002544 KIAA1919 KIAA1919 2 1
MIRT002545 SLC25A23 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 23 2 1
MIRT002548 C9orf78 chromosome 9 open reading frame 78 2 1
MIRT002552 KDM1B lysine (K)-specific demethylase 1B 2 1
MIRT002554 MED28 mediator complex subunit 28 2 1
MIRT002555 ADAM9 ADAM metallopeptidase domain 9 (meltrin gamma) 2 1
MIRT002556 TTC8 tetratricopeptide repeat domain 8 2 1
MIRT002558 ZHX1 zinc fingers and homeoboxes 1 2 1
MIRT002559 RPIA ribose 5-phosphate isomerase A 2 1
MIRT003815 PCGF5 polycomb group ring finger 5 2 1
MIRT003816 PDIK1L PDLIM1 interacting kinase 1 like 2 1
MIRT003817 UBXN1 UBX domain protein 1 2 1
MIRT003832 CDK19 cyclin-dependent kinase 19 2 1
MIRT003843 CMTM4 CKLF-like MARVEL transmembrane domain containing 4 2 1
MIRT003846 TMEM9B TMEM9 domain family, member B 2 1
MIRT003914 RAD52 RAD52 homolog (S. cerevisiae) 4 1
MIRT003918 XPA xeroderma pigmentosum, complementation group A 2 1
MIRT003919 MRE11A MRE11 meiotic recombination 11 homolog A (S. cerevisiae) 2 1
MIRT003942 PACRGL PARK2 co-regulated-like 2 1
MIRT003943 TEX30 chromosome 13 open reading frame 27 2 1
MIRT003944 C12orf23 chromosome 12 open reading frame 23 2 1
MIRT003948 ANKRD13B ankyrin repeat domain 13B 2 1
MIRT004073 MARCH4 membrane-associated ring finger (C3HC4) 4 2 1
MIRT004074 CNOT6 CCR4-NOT transcription complex, subunit 6 2 1
MIRT004438 CYB5R4 cytochrome b5 reductase 4 2 1
MIRT004448 VPS26A vacuolar protein sorting 26 homolog A (S. pombe) 2 1
MIRT004449 PBK PDZ binding kinase 2 1
MIRT004468 FAM13B family with sequence similarity 13, member B 2 1
MIRT004515 SEPT2 septin 2 2 1
MIRT005561 TXNIP thioredoxin interacting protein 4 1
MIRT005562 RABEP1 rabaptin, RAB GTPase binding effector protein 1 4 1
MIRT006016 MYC v-myc myelocytomatosis viral oncogene homolog (avian) 3 1
MIRT006021 MBD2 methyl-CpG binding domain protein 2 3 1
MIRT006022 RASSF1 Ras association (RalGDS/AF-6) domain family member 1 3 1
MIRT006157 MTOR mechanistic target of rapamycin (serine/threonine kinase) 3 1
MIRT006158 SIRT1 sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) 3 1
MIRT006735 NFIB nuclear factor I/B 4 1
MIRT020072 CD24P4 CD24 molecule-like 4 pseudogene 1 1
MIRT035565 ANKRD52 ankyrin repeat domain 52 1 1