Accession ID: MIRT003967 [miRNA, hsa-miR-212-3p :: BRCA1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-212LinkOut: [miRBase ]
Synonyms MIRN212, mir-212, MIR212
Description Homo sapiens miR-212 stem-loop
Comment This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences . Expression of the excised miR has been validated in zebrafish, and the 5' end mapped by PCR. The 3' end was not experimentally determined. The sequence maps to human chromosome 17, but its expression has not been experimentally verified in human.
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-212-3p
Mature Sequence 71| UAACAGUCUCCAGUCACGGCC |91
Evidence Not_experimental
Experiments
Putative hsa-miR-212-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol BRCA1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PPP1R53, PSCP, RNF53
Description breast cancer 1, early onset
Transcript NM_007299    LinkOut: [ RefSeq ]
Other Transcripts NM_007297 , NM_007298 , NM_007294 , NM_007300   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on BRCA1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of BRCA1
(miRNA target sites are highlighted)
>BRCA1|NM_007299|3'UTR
   1 GGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATAC
  81 CCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAG
 161 TGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCA
 241 GCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGC
 321 ACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTCATT
 401 ATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAAT
 481 AGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGT
 561 GCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGA
 641 AATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTT
 721 GGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACT
 801 AAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACT
 881 TCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTC
 961 AAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGA
1041 ATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAA
1121 ATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCTATA
1201 AAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCA
1281 TACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGC
1361 TGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTA
1441 AATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' ccggCACUGAC-CUCUGACAau 5'
              ||||| | ||||||||  
Target 5' ctggGTGACAGTGAGACTGTgg 3'
936 - 957 149.00 -17.80
2
miRNA  3' ccGGCACUGACCUCUGACAAu 5'
            || | |:   :||||||| 
Target 5' acCCATTATCCAGGACTGTTt 3'
1335 - 1355 147.00 -9.03
3
miRNA  3' ccggcacugaccUCUGACAAu 5'
                      ||:||||| 
Target 5' ttctttctattcAGGCTGTTg 3'
415 - 435 129.00 -9.40
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-212-3p :: BRCA1    [ Functional MTI ]
Validation Method Microarray , Other
Conditions normal and malignant ovarian tissues
Original Description (Extracted from the article) ... Confirmed and potential targets for miRNA members of various signatures.//{This MTI shown in Supplementary Table S6} ...

- Iorio, M. V. Visone, R. Di Leva, G. Donati, et al., 2007, Cancer Res.

Article - Iorio, M. V. Visone, R. Di Leva, G. Donati, et al.
- Cancer Res, 2007
Epithelial ovarian cancer (EOC) is the sixth most common cancer in women worldwide and, despite advances in detection and therapies, it still represents the most lethal gynecologic malignancy in the industrialized countries. Unfortunately, still relatively little is known about the molecular events that lead to the development of this highly aggressive disease. The relatively recent discovery of microRNAs (miRNA), a class of small noncoding RNAs targeting multiple mRNAs and triggering translation repression and/or RNA degradation, has revealed the existence of a new level of gene expression regulation. Multiple studies involving various types of human cancers proved that miRNAs have a causal role in tumorigenesis. Here we show that, in comparison to normal ovary, miRNAs are aberrantly expressed in human ovarian cancer. The overall miRNA expression could clearly separate normal versus cancer tissues. The most significantly overexpressed miRNAs were miR-200a, miR-141, miR-200c, and miR-200b, whereas miR-199a, miR-140, miR-145, and miR-125b1 were among the most down-modulated miRNAs. We could also identify miRNAs whose expression was correlated with specific ovarian cancer biopathologic features, such as histotype, lymphovascular and organ invasion, and involvement of ovarian surface. Moreover, the levels of miR-21, miR-203, and miR-205, up-modulated in ovarian carcinomas compared with normal tissues, were significantly increased after 5-aza-2'-deoxycytidine demethylating treatment of OVCAR3 cells, suggesting that the DNA hypomethylation could be the mechanism responsible for their overexpression. Our results indicate that miRNAs might play a role in the pathogenesis of human EOC and identify altered miRNA gene methylation as a possible epigenetic mechanism involved in their aberrant expression.
LinkOut: [PMID: 17875710]
MiRNA-Target Expression Profile:

 
MiRNA-Target Expression Profile(TCGA):

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
78 hsa-miR-212-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT003897 MECP2 methyl CpG binding protein 2 (Rett syndrome) 5 1
MIRT003966 WT1 Wilms tumor 1 2 1
MIRT003967 BRCA1 breast cancer 1, early onset 2 1
MIRT004341 TJP1 tight junction protein 1 3 1
MIRT004501 PEA15 phosphoprotein enriched in astrocytes 15 4 1
MIRT006293 PTCH1 patched 1 3 2
MIRT006504 RB1 retinoblastoma 1 3 1
MIRT007044 KCNJ2 potassium inwardly-rectifying channel, subfamily J, member 2 2 1
MIRT024968 CCNA2 cyclin A2 2 1
MIRT024969 CCNB1 cyclin B1 2 1
MIRT047000 DPF1 D4, zinc and double PHD fingers family 1 1 1
MIRT047001 AGO1 eukaryotic translation initiation factor 2C, 1 1 1
MIRT053351 RBP2 retinol binding protein 2, cellular 3 2
MIRT053469 MYC v-myc myelocytomatosis viral oncogene homolog (avian) 3 1
MIRT091254 FXR1 fragile X mental retardation, autosomal homolog 1 1 2
MIRT100365 HSPA1B heat shock 70kDa protein 1B 1 1
MIRT100692 TJAP1 tight junction associated protein 1 (peripheral) 1 1
MIRT106173 RRS1 RRS1 ribosome biogenesis regulator homolog (S. cerevisiae) 1 1
MIRT149853 LDLR low density lipoprotein receptor 1 1
MIRT217678 ASF1A ASF1 anti-silencing function 1 homolog A (S. cerevisiae) 1 1
MIRT259586 EIF2S3 eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa 1 1
MIRT289306 TNRC6C trinucleotide repeat containing 6C 1 1
MIRT368373 ARID2 AT rich interactive domain 2 (ARID, RFX-like) 1 1
MIRT437838 SOD2 superoxide dismutase 2, mitochondrial 1 1
MIRT438683 ACHE acetylcholinesterase 3 1
MIRT438881 IRAK4 interleukin-1 receptor-associated kinase 4 1 1
MIRT442188 HOOK3 hook homolog 3 (Drosophila) 1 1
MIRT449482 STAG1 stromal antigen 1 1 1
MIRT453130 HOXC4 homeobox C4 1 1
MIRT455371 KDM5C lysine (K)-specific demethylase 5C 1 1
MIRT455525 C6orf106 chromosome 6 open reading frame 106 1 1
MIRT458881 PFAS phosphoribosylformylglycinamidine synthase 1 1
MIRT464181 VMP1 vacuole membrane protein 1 1 10
MIRT467689 SLC38A2 solute carrier family 38, member 2 1 2
MIRT476655 FZD6 frizzled family receptor 6 1 2
MIRT477438 EML4 echinoderm microtubule associated protein like 4 1 2
MIRT479293 CHAC1 ChaC, cation transport regulator homolog 1 (E. coli) 1 1
MIRT485290 PLAGL2 pleiomorphic adenoma gene-like 2 1 1
MIRT495257 BMPER BMP binding endothelial regulator 1 1
MIRT497152 PRDM15 PR domain containing 15 1 1
MIRT500829 TCEB1 transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C) 1 1
MIRT506947 HS3ST3B1 heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 1 2
MIRT507624 CRTC1 CREB regulated transcription coactivator 1 1 1
MIRT508136 AMD1 adenosylmethionine decarboxylase 1 1 1
MIRT521605 PSMA2 proteasome (prosome, macropain) subunit, alpha type, 2 1 2
MIRT526270 CCDC169 coiled-coil domain containing 169 1 1
MIRT526866 PARP11 poly (ADP-ribose) polymerase family, member 11 1 1
MIRT532418 ART4 ADP-ribosyltransferase 4 (Dombrock blood group) 1 1
MIRT532507 TWISTNB TWIST neighbor 1 2
MIRT534832 RAB18 RAB18, member RAS oncogene family 1 1
MIRT542576 ZNF280B zinc finger protein 280B 1 1
MIRT546159 TSPAN12 tetraspanin 12 1 1
MIRT547254 NUP50 nucleoporin 50kDa 1 2
MIRT547613 LIFR leukemia inhibitory factor receptor alpha 1 1
MIRT547706 KPNA1 karyopherin alpha 1 (importin alpha 5) 1 2
MIRT549142 BRWD1 bromodomain and WD repeat domain containing 1 1 1
MIRT549454 ACSL4 acyl-CoA synthetase long-chain family member 4 1 1
MIRT551639 TRUB1 TruB pseudouridine (psi) synthase homolog 1 (E. coli) 1 2
MIRT554984 RAB5B RAB5B, member RAS oncogene family 1 1
MIRT555923 OCLN occludin 1 2
MIRT557609 GNB1 guanine nucleotide binding protein (G protein), beta polypeptide 1 1 1
MIRT571511 ZNF711 zinc finger protein 711 1 1
MIRT572927 PRAMEF1 PRAME family member 1 1 1
MIRT573616 ZNF724P zinc finger protein 724, pseudogene 1 1
MIRT574925 Vmp1 vacuole membrane protein 1 1 1
MIRT614464 ALKBH4 alkB, alkylation repair homolog 4 (E. coli) 1 1
MIRT623024 OLFML2A olfactomedin-like 2A 1 1
MIRT639079 CD226 CD226 molecule 1 1
MIRT642133 CYP20A1 cytochrome P450, family 20, subfamily A, polypeptide 1 1 1
MIRT653733 SLC25A32 solute carrier family 25 (mitochondrial folate carrier) , member 32 1 1
MIRT655168 PHF20L1 PHD finger protein 20-like 1 1 1
MIRT680988 DCAF17 DDB1 and CUL4 associated factor 17 1 1
MIRT681563 UBXN2A UBX domain protein 2A 1 1
MIRT696293 IER3IP1 immediate early response 3 interacting protein 1 1 1
MIRT697725 USP8 ubiquitin specific peptidase 8 1 1
MIRT703295 GID4 GID complex subunit 4, VID24 homolog (S. cerevisiae) 1 1
MIRT718421 CALN1 calneuron 1 1 1
MIRT719459 APBA1 amyloid beta (A4) precursor protein-binding, family A, member 1 1 1
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