Accession ID: MIRT003967 [miRNA, hsa-miR-212-3p :: BRCA1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-212LinkOut: [miRBase ]
Synonyms MIRN212, mir-212, MIR212
Description Homo sapiens miR-212 stem-loop
Comment This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences . Expression of the excised miR has been validated in zebrafish, and the 5' end mapped by PCR. The 3' end was not experimentally determined. The sequence maps to human chromosome 17, but its expression has not been experimentally verified in human.
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-212-3p
Mature Sequence 71| UAACAGUCUCCAGUCACGGCC |91
Evidence Not_experimental
Experiments
Putative hsa-miR-212-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol BRCA1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PSCP, RNF53
Description breast cancer 1, early onset
Transcript NM_0072    LinkOut: [ RefSeq ]
Other Transcripts NM_0072 , NM_0072 , NM_0072 , NM_0073   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on BRCA1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of BRCA1
(miRNA target sites are highlighted)
>BRCA1|NM_0072|3'UTR
   1 TGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGA
  81 TACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACA
 161 AAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACA
 241 TCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATG
 321 AGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTC
 401 ATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAG
 481 AATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAG
 561 AGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGA
 641 GGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCAC
 721 TTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCT
 801 ACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATC
 881 ACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGG
 961 CTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAA
1041 AGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGT
1121 AAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCT
1201 ATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAA
1281 CCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTAT
1361 AGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTT
1441 GTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCAAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' ccggCACUGAC-CUCUGACAau 5'
              ||||| | ||||||||  
Target 5' ctggGTGACAGTGAGACTGTgg 3'
939 - 960 149.00 -17.80
2
miRNA  3' ccGGCACUGACCUCUGACAAu 5'
            || | |:   :||||||| 
Target 5' acCCATTATCCAGGACTGTTt 3'
1338 - 1358 147.00 -9.03
3
miRNA  3' ccggcacugaccUCUGACAAu 5'
                      ||:||||| 
Target 5' ttctttctattcAGGCTGTTg 3'
418 - 438 129.00 -9.40
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-212-3p :: BRCA1    [ Functional MTI ]
Validation Method Microarray , Other
Conditions normal and malignant ovarian tissues
Original Description (Extracted from the article) ... Confirmed and potential targets for miRNA members of various signatures.//{This MTI shown in Supplementary Table S6} ...

- Iorio, M. V. Visone, R. Di Leva, G. Donati, et al., 2007, Cancer Res.

Article - Iorio, M. V. Visone, R. Di Leva, G. Donati, et al.
- Cancer Res, 2007
Epithelial ovarian cancer (EOC) is the sixth most common cancer in women worldwide and, despite advances in detection and therapies, it still represents the most lethal gynecologic malignancy in the industrialized countries. Unfortunately, still relatively little is known about the molecular events that lead to the development of this highly aggressive disease. The relatively recent discovery of microRNAs (miRNA), a class of small noncoding RNAs targeting multiple mRNAs and triggering translation repression and/or RNA degradation, has revealed the existence of a new level of gene expression regulation. Multiple studies involving various types of human cancers proved that miRNAs have a causal role in tumorigenesis. Here we show that, in comparison to normal ovary, miRNAs are aberrantly expressed in human ovarian cancer. The overall miRNA expression could clearly separate normal versus cancer tissues. The most significantly overexpressed miRNAs were miR-200a, miR-141, miR-200c, and miR-200b, whereas miR-199a, miR-140, miR-145, and miR-125b1 were among the most down-modulated miRNAs. We could also identify miRNAs whose expression was correlated with specific ovarian cancer biopathologic features, such as histotype, lymphovascular and organ invasion, and involvement of ovarian surface. Moreover, the levels of miR-21, miR-203, and miR-205, up-modulated in ovarian carcinomas compared with normal tissues, were significantly increased after 5-aza-2'-deoxycytidine demethylating treatment of OVCAR3 cells, suggesting that the DNA hypomethylation could be the mechanism responsible for their overexpression. Our results indicate that miRNAs might play a role in the pathogenesis of human EOC and identify altered miRNA gene methylation as a possible epigenetic mechanism involved in their aberrant expression.
LinkOut: [PMID: 17875710]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
12 hsa-miR-212-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT003897 MECP2 methyl CpG binding protein 2 (Rett syndrome) 5 1
MIRT003966 WT1 Wilms tumor 1 2 1
MIRT003967 BRCA1 breast cancer 1, early onset 2 1
MIRT004341 TJP1 tight junction protein 1 (zona occludens 1) 3 1
MIRT004501 PEA15 phosphoprotein enriched in astrocytes 15 4 1
MIRT006293 PTCH1 patched homolog 1 (Drosophila) 3 1
MIRT006504 RB1 retinoblastoma 1 3 1
MIRT007044 KCNJ2 potassium inwardly-rectifying channel, subfamily J, member 2 2 1
MIRT024968 CCNA2 cyclin A2 2 1
MIRT024969 CCNB1 cyclin B1 2 1
MIRT047000 DPF1 D4, zinc and double PHD fingers family 1 1 1
MIRT047001 AGO1 eukaryotic translation initiation factor 2C, 1 1 1