Accession ID: MIRT004050 [miRNA, hsa-miR-129-5p :: ETV6, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-129-1LinkOut: [miRBase ]
Description Homo sapiens miR-129-1 stem-loop
Comment This miRNA sequence was predicted based on homology to a verified miRNA cloned from mouse cerebellum .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-129-5p
Mature Sequence 5| CUUUUUGCGGUCUGGGCUUGC |25
Evidence Experimental
Experiments Cloned
Putative hsa-miR-129-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol ETV6 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms TEL, TEL/ABL
Description ets variant 6
Transcript NM_0019    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on ETV6 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of ETV6
(miRNA target sites are highlighted)
>ETV6|NM_0019|3'UTR
   1 AGGAACCAACAGTCCACCTCAGCGGGCCAGCAGCCCAGGGAACCCCTGCCCACCAGGATTGCTGGAAGTGTGACGGAGCA
  81 GGCGGGCTGAGGAGAGTGGAAAAGGAAGCGACCCAGAAATGGCAGGGACACTTCTCTTGCAGACCAAGAGGGACCCTGGA
 161 GCACCTTAGACAAACTACCCAGCACAGGCGGGGCTGGAATTCTGGCGGAGGGCATGAGCCTGGGACTCCATGTCACGTTT
 241 CCTTCTGATTTGGAATCTCTCCATCTGTAATTCCTCACCCTCACCCTTCCACCGTTGTTAGTATCATGGTGTTTTTGTTT
 321 TTGTTTTTGTTTTAAGAACCTGCAGTTTGACTCTTCATCGTTCATCTAGGGGAAGACATCTGATGTTGTTTTCCTATGGA
 401 AATATATATCTATTATATATATATTTTTTGCAAATCTCACAAAGTGCGGCAAGCCCAGCTGGTCAGGAAAGAGAATACTT
 481 GCAGAGGGGTTCAGGTTCCTCTTTTTCCTGCCACGTGGATCAGGTCTGTTCCTGTTACTGTTGGGTCTTGGCTGAAAAAA
 561 AAAAATGCTTTTAAAAAAGATAAAATGAAAAGGAGAGCTCTCTTTTTCTCTCTCTTGCTCTGTTCTTCCCTTGGTCCCCT
 641 CTGTCCTCCCGCCCTGCCTGCAGTTGAGATTCAGATGCCTTCTGACAGAGTTCAGCCTCTTGGAGAGTCTTGGGGATTGT
 721 TGGCACCTAAACAGAATCAGTGACCCGGGTGCTTTGTGGCCAGCAGCACAGAATCAAACCCGCATCCCAGCATTGGGCCA
 801 CCCATCTGAGGGAGGCCAAAATCATCACAGATGCTGCTGTGCTGCAGACAGATACATGCTAGTCCAGAGAGCCGCCCCTG
 881 AGATGGCTGTGAGAACCATGTGTCTAAGGCGTAAGATAAGGATGGAAGGCTGTCCAAGTTATTTGGAAGGCCTCGGCAGC
 961 TTGGGATTAGCTTGGGAGCGCAGCGCTGCAAAGTGGAAAATATGAAAAGACCACACAGGCCCAGCAGTCCAGAAACTGGG
1041 CAAAAATATTCTGCAGTGGGGATTTATTTTTCCAAAGCAGGTAACAGAGGCTAGTGAGAAAGAAAAGCTCCTCTCTGCTC
1121 CATTCCAAAGGCCATCTTGTGGTCAGTTTCATGCCCTCACCTGATTTTTTTTTTTTTTTTTTTTTTTCAATTCCTAACCT
1201 TTTTTAAAGTTTCCTGGTCTCCACTGGACACAGAGCTTTGGAGACGGAGGATCCCAGAGGGCAGTCTCAGTTGCAATCAG
1281 TGTGTGCCCAGCCTGGGCAGACAGGAAATTCCTCGGATACATTATTTTTTCTTTCTTTCATAGCTGTGTCTCAGAAAGGA
1361 CCCATTTGTGGCTCTTTTTCACCTCAAAATAAGATCGATGGTATCTTGTAAAATGAGGGTAGTGCCACTTCTTAGTATTT
1441 TTGAAAGCTGTTTTAGATTTTTTTTTTTTTTCCTTTTCTAGCCATCTAAATTGACTCTTCCAATATAGGTCTCAGAAATC
1521 CAATATTTGGAGTACAATTTCTTTTAATCCAGATTACACCTGCCTTACAAAGCACCCCCTCCTTGTTCCCCTCTGTTTCC
1601 TCTACTCAGTTGGGGGAGAAACTCACAGCTCCTCCGGGATACATATGTGCCCTCAGCAGCAGCTCCCAGGTGAAGTTACC
1681 AGACCCCTGGGCTTCTCCCCAGCTTTTTCTGAGTTGAGTCAGACATGTAGAGTTTGGGTCACACAGGCAAGAGGAATTTT
1761 CCCTCGGCCTTACTGACAAGGACACCAACCTAGGGTGCAAACAGATGGACTATGGTTCAAGGACACTGGAATTGAGGAGC
1841 TGATCAAGGCTCTCTTCAGCCTTGCTCTGTCCCTGCCTCTTATCAGAGCACAGGTAGACACACGGGCATAGCCAGCCCAC
1921 TCCTACTGTCACAGGCGCCCCACCATTCAACCTTCCGGGAGGTCAGGGACCTTCTATATGAGGCGAGTGGGTCTCAGTCT
2001 GCTTGAATGGTGATGAGATTCTGCTGGATCTCAGCACGCTGCAGGTGTCTTTTGAGAGCATTCAGTAGGACATGGTGATC
2081 CCTATTTCAGCCTCTAAGATGACTGGTATTCTATCTGAAATGCAGAGATTAAGCCAAATACCTGATGTATTGTGAAAGCC
2161 ACTGATTTTAAGAATGGAGAGAAAGGGATTTTTTACTGCATCCCTCTGTATGAATATGAAATCAGAGACCAGGGCATGAT
2241 GTTGCTAGGATTAGAGCCTCTCAGTCTGGCCTCTTCACCCAAGTGCAAGAACTCAGTCTCTTACTGTTCAAAGAATCTTA
2321 ACAGTTGAATTATGGAGGGAAATTCCCTTTTGCCCCAAGCATTTCTATATTTAAAGCAATATCCCAGGAGAATATGTTAG
2401 ACTTAGGATGATACCTTCAGCCACTTGAAGAAGAAATAGAAGGCGCTCATTCCAATATAGTCTTTATTTCCCATTCAGAT
2481 ACAGGTTGAGCATCCCTAATCTGAACAGTTAAAACCCCCAAATGCCCCAAAATCCAAACCTTCCTGAACGCTATGACACC
2561 ATGAGTGGAAAATTCCACACCTAACAAACACATTTGCTTTCTTATGGTTCAATGTACACAAACTGTTTTATATAGAAAAT
2641 GATTTCAAATATCATAAAATTACCTTCAGGCTATGTGTATAAAGTATATATGAGCCATAAATGAATTTTGTGTTTAGACT
2721 TTGTGTCCATCCCCAAGATCTCTCATTTTATATATATATATATATATATATATATATATATATATATATATATATACACA
2801 CACACACACATACACAAATATTCCAGGATACAAAAAAAAACATTTAAAAATCCGAGACCCAGAACACTTCTGGTCCCAAG
2881 CATTTCAGATAAGGGATATCAATCTGTACTACCAATAAGGATTTCGTAATTCCCCTAACTGCAAATGTCCTCTTCATTTG
2961 TTCTTTATGAGAAAACCCGGGTAGTGCCAGCACCTGGATACAGTATTTACACCCTGCAGACCCTAAAGATTTCAGATTCA
3041 GTTAGCAAACCTTGATGAAGCACCTGCTGGACACTGAGGGACCCAAAGCTCAATCAGCCATAATCCCTGCTTTCAGAGTT
3121 TATATTGTACCTGCCTAATCCACCCGGCGTGACTCATTTCAACACTAAGTACTAGGGGTGTTGTCAGGAGACAAATCTGA
3201 AGTCAGGAGAGGAAAATGCAAAGGAGCCCTGCCGTGTGATGGATGTGCATTCTCACTTGGGTCTTGAAGTTCTCATTCCT
3281 ACATCTCAAGCTAGCCAGGCAGTCTCCTCTCTATCAGAAGAAAGCACTGGTAATTGGCTAGACTGGCTATGTTGAAGGTA
3361 ACATGAACTCTAAGATCTTGACCCAGGGCGACTTGGTTTTGCTTAAGGTGGCATCACCAATGTTCCAAATCCTTTAGGGA
3441 GATGAGGGTATCCCCACAGAAAAAGAGGAATAATAGACCAATGGATTTTCTCCTTTCACCAGTATGTTTGGAACCCTCTG
3521 ATCCAATGTCTTTTGATACTGATCTCTTGTCCAAATGAGAATGTCGCTTTAGCTGAAATTCAAATGGCTGTGACAATTTA
3601 CCGAAATGATGAAGTAACCACCATTCCCACCTTTCACTGCCTAGGCTCCAAGTCTGAATACATTTTTGAAATAGGAACTC
3681 CCTTTTGCAAAAAAGAAACCTGGGTGTCAGGGAGGTGAAGTGACTTGCCCTAGGAGCAGACAGCATGCCAAGAATGGAAT
3761 TAGGCTCAGGATCCAGCCTGGGCTCACCCTGTGTGGCTCATTCCCACCCAGGAAACTGAAGATAAAAGATTTGGGAAAAC
3841 ACACCAAGAAAAAGGGGCAGTTTTCTTTGCCCAAGCATTTGGTGCTAGTTAGAGGCTGTTCACTCTCTCCTGCTCCTCTT
3921 CGGAGTAGAAATAAAGGCTGTGACACAAGGAAGCCAGTGGGGTGGGAGGGAGGCACCATAATCCCTCCCTAAAACCCACA
4001 GAAGACTAACCTGATACTCTTTTGACCCAACTGCATCAACACTAAACAGCTGCAGACCCCCTGAATCTTTCACACATGCC
4081 AAGTGAACATTCTTGATGATTTCTCTTTGTGACCGCAACCACCTGCAAACCAGAACGACTCTAGAATTTCCTTCCCCGCC
4161 CCCCTTTTTGTTTAGTTTCTAATCTCTTGTTTATGAGGTGTGGGGTTTATAAGGGACTGAATCAAATGAATGTAACAAAA
4241 AAGAAAAAAAAAACAAAAAAAAATGCCTTTTCTCAGGGCCAGTGAGTTGCAAATAATTTTTAAAGAAAAGCCTATAATTA
4321 CATCATCTCAATAAATTTTTTATAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' cguUCGGGUC--UGG--CGUUUUUc 5'
             ||:||||  ||:  ||||||| 
Target 5' agcAGTCCAGAAACTGGGCAAAAAt 3'
1023 - 1047 162.00 -15.70
2
miRNA  3' cgUUCGGGUCUGGCGUUUUUc 5'
            || :||   ::||||||| 
Target 5' ggAACTCCCTTTTGCAAAAAa 3'
3674 - 3694 151.00 -11.50
3
miRNA  3' cguucGGGUCUGGCGUUUUUc 5'
               |||| | :||||:|| 
Target 5' cttcaCCCA-AGTGCAAGAAc 3'
2273 - 2292 139.00 -12.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-129-5p :: ETV6    [ Functional MTI ]
Validation Method In situ hybridization , Microarray , qRT-PCR , Other
Conditions T24 , SW780 , HT1376 , RT4 , J82 , HU609 , HCV29
Disease bladder cancer;
Location of target site 3'UTR
Tools used in this research PicTar , TargetScan
Original Description (Extracted from the article) ... GALNT1 and SOX4 both showed significant reduction in luciferase activity on transfection with pre-miR-129 compared with GALNT1 and SOX4 3'-UTRs with mutated miR-129 target sites.// ...

- Dyrskjot, L. Ostenfeld, M. S. Bramsen, J. et al., 2009, Cancer Res.

Article - Dyrskjot, L. Ostenfeld, M. S. Bramsen, J. et al.
- Cancer Res, 2009
microRNAs (miRNA) are involved in cancer development and progression, acting as tumor suppressors or oncogenes. Here, we profiled the expression of 290 unique human miRNAs in 11 normal and 106 bladder tumor samples using spotted locked nucleic acid-based oligonucleotide microarrays. We identified several differentially expressed miRNAs between normal urothelium and cancer and between the different disease stages. miR-145 was found to be the most down-regulated in cancer compared with normal, and miR-21 was the most up-regulated in cancer. Furthermore, we identified miRNAs that significantly correlated to the presence of concomitant carcinoma in situ. We identified several miRNAs with prognostic potential for predicting disease progression (e.g., miR-129, miR-133b, and miR-518c*). We localized the expression of miR-145, miR-21, and miR-129 to urothelium by in situ hybridization. We then focused on miR-129 that exerted significant growth inhibition and induced cell death upon transfection with a miR-129 precursor in bladder carcinoma cell lines T24 and SW780 cells. Microarray analysis of T24 cells after transfection showed significant miR-129 target down-regulation (P = 0.0002) and pathway analysis indicated that targets were involved in cell death processes. By analyzing gene expression data from clinical tumor samples, we identified significant expression changes of target mRNA molecules related to the miRNA expression. Using luciferase assays, we documented a direct link between miR-129 and the two putative targets GALNT1 and SOX4. The findings reported here indicate that several miRNAs are differentially regulated in bladder cancer and may form a basis for clinical development of new biomarkers for bladder cancer.
LinkOut: [PMID: 19487295]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
16 hsa-miR-129-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001953 CAMTA1 calmodulin binding transcription activator 1 2 1
MIRT001954 EIF2C3 eukaryotic translation initiation factor 2C, 3 2 1
MIRT004043 SOX4 SRY (sex determining region Y)-box 4 5 3
MIRT004049 GALNT1 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) 3 2
MIRT004050 ETV6 ets variant 6 3 1
MIRT004051 BMPR2 bone morphogenetic protein receptor, type II (serine/threonine kinase) 3 1
MIRT005412 NOTCH1 notch 1 3 1
MIRT006660 UBE2F ubiquitin-conjugating enzyme E2F (putative) 1 1
MIRT007376 FMR1 fragile X mental retardation 1 1 1
MIRT026018 PDS5A PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae) 1 1
MIRT026019 TNPO1 transportin 1 1 1
MIRT026020 TP53INP1 tumor protein p53 inducible nuclear protein 1 1 1
MIRT026021 FNDC3B fibronectin type III domain containing 3B 1 1
MIRT026022 ZFP91 zinc finger protein 91 homolog (mouse) 1 1
MIRT048045 NSMF nasal embryonic LHRH factor 1 1
MIRT048046 HIST1H2BJ histone cluster 1, H2bj 1 1