Accession ID: MIRT004050 [miRNA, hsa-miR-129-5p :: ETV6, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-129-1LinkOut: [miRBase ]
Description Homo sapiens miR-129-1 stem-loop
Comment This miRNA sequence was predicted based on homology to a verified miRNA cloned from mouse cerebellum .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-129-5p
Mature Sequence 5| CUUUUUGCGGUCUGGGCUUGC |25
Evidence Experimental
Experiments Cloned
Putative hsa-miR-129-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol ETV6 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms TEL, TEL/ABL
Description ets variant 6
Transcript NM_0019    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on ETV6 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of ETV6
(miRNA target sites are highlighted)
>ETV6|NM_0019|3'UTR
   1 TGAAGGAACCAACAGTCCACCTCAGCGGGCCAGCAGCCCAGGGAACCCCTGCCCACCAGGATTGCTGGAAGTGTGACGGA
  81 GCAGGCGGGCTGAGGAGAGTGGAAAAGGAAGCGACCCAGAAATGGCAGGGACACTTCTCTTGCAGACCAAGAGGGACCCT
 161 GGAGCACCTTAGACAAACTACCCAGCACAGGCGGGGCTGGAATTCTGGCGGAGGGCATGAGCCTGGGACTCCATGTCACG
 241 TTTCCTTCTGATTTGGAATCTCTCCATCTGTAATTCCTCACCCTCACCCTTCCACCGTTGTTAGTATCATGGTGTTTTTG
 321 TTTTTGTTTTTGTTTTAAGAACCTGCAGTTTGACTCTTCATCGTTCATCTAGGGGAAGACATCTGATGTTGTTTTCCTAT
 401 GGAAATATATATCTATTATATATATATTTTTTGCAAATCTCACAAAGTGCGGCAAGCCCAGCTGGTCAGGAAAGAGAATA
 481 CTTGCAGAGGGGTTCAGGTTCCTCTTTTTCCTGCCACGTGGATCAGGTCTGTTCCTGTTACTGTTGGGTCTTGGCTGAAA
 561 AAAAAAAATGCTTTTAAAAAAGATAAAATGAAAAGGAGAGCTCTCTTTTTCTCTCTCTTGCTCTGTTCTTCCCTTGGTCC
 641 CCTCTGTCCTCCCGCCCTGCCTGCAGTTGAGATTCAGATGCCTTCTGACAGAGTTCAGCCTCTTGGAGAGTCTTGGGGAT
 721 TGTTGGCACCTAAACAGAATCAGTGACCCGGGTGCTTTGTGGCCAGCAGCACAGAATCAAACCCGCATCCCAGCATTGGG
 801 CCACCCATCTGAGGGAGGCCAAAATCATCACAGATGCTGCTGTGCTGCAGACAGATACATGCTAGTCCAGAGAGCCGCCC
 881 CTGAGATGGCTGTGAGAACCATGTGTCTAAGGCGTAAGATAAGGATGGAAGGCTGTCCAAGTTATTTGGAAGGCCTCGGC
 961 AGCTTGGGATTAGCTTGGGAGCGCAGCGCTGCAAAGTGGAAAATATGAAAAGACCACACAGGCCCAGCAGTCCAGAAACT
1041 GGGCAAAAATATTCTGCAGTGGGGATTTATTTTTCCAAAGCAGGTAACAGAGGCTAGTGAGAAAGAAAAGCTCCTCTCTG
1121 CTCCATTCCAAAGGCCATCTTGTGGTCAGTTTCATGCCCTCACCTGATTTTTTTTTTTTTTTTTTTTTTTCAATTCCTAA
1201 CCTTTTTTAAAGTTTCCTGGTCTCCACTGGACACAGAGCTTTGGAGACGGAGGATCCCAGAGGGCAGTCTCAGTTGCAAT
1281 CAGTGTGTGCCCAGCCTGGGCAGACAGGAAATTCCTCGGATACATTATTTTTTCTTTCTTTCATAGCTGTGTCTCAGAAA
1361 GGACCCATTTGTGGCTCTTTTTCACCTCAAAATAAGATCGATGGTATCTTGTAAAATGAGGGTAGTGCCACTTCTTAGTA
1441 TTTTTGAAAGCTGTTTTAGATTTTTTTTTTTTTTCCTTTTCTAGCCATCTAAATTGACTCTTCCAATATAGGTCTCAGAA
1521 ATCCAATATTTGGAGTACAATTTCTTTTAATCCAGATTACACCTGCCTTACAAAGCACCCCCTCCTTGTTCCCCTCTGTT
1601 TCCTCTACTCAGTTGGGGGAGAAACTCACAGCTCCTCCGGGATACATATGTGCCCTCAGCAGCAGCTCCCAGGTGAAGTT
1681 ACCAGACCCCTGGGCTTCTCCCCAGCTTTTTCTGAGTTGAGTCAGACATGTAGAGTTTGGGTCACACAGGCAAGAGGAAT
1761 TTTCCCTCGGCCTTACTGACAAGGACACCAACCTAGGGTGCAAACAGATGGACTATGGTTCAAGGACACTGGAATTGAGG
1841 AGCTGATCAAGGCTCTCTTCAGCCTTGCTCTGTCCCTGCCTCTTATCAGAGCACAGGTAGACACACGGGCATAGCCAGCC
1921 CACTCCTACTGTCACAGGCGCCCCACCATTCAACCTTCCGGGAGGTCAGGGACCTTCTATATGAGGCGAGTGGGTCTCAG
2001 TCTGCTTGAATGGTGATGAGATTCTGCTGGATCTCAGCACGCTGCAGGTGTCTTTTGAGAGCATTCAGTAGGACATGGTG
2081 ATCCCTATTTCAGCCTCTAAGATGACTGGTATTCTATCTGAAATGCAGAGATTAAGCCAAATACCTGATGTATTGTGAAA
2161 GCCACTGATTTTAAGAATGGAGAGAAAGGGATTTTTTACTGCATCCCTCTGTATGAATATGAAATCAGAGACCAGGGCAT
2241 GATGTTGCTAGGATTAGAGCCTCTCAGTCTGGCCTCTTCACCCAAGTGCAAGAACTCAGTCTCTTACTGTTCAAAGAATC
2321 TTAACAGTTGAATTATGGAGGGAAATTCCCTTTTGCCCCAAGCATTTCTATATTTAAAGCAATATCCCAGGAGAATATGT
2401 TAGACTTAGGATGATACCTTCAGCCACTTGAAGAAGAAATAGAAGGCGCTCATTCCAATATAGTCTTTATTTCCCATTCA
2481 GATACAGGTTGAGCATCCCTAATCTGAACAGTTAAAACCCCCAAATGCCCCAAAATCCAAACCTTCCTGAACGCTATGAC
2561 ACCATGAGTGGAAAATTCCACACCTAACAAACACATTTGCTTTCTTATGGTTCAATGTACACAAACTGTTTTATATAGAA
2641 AATGATTTCAAATATCATAAAATTACCTTCAGGCTATGTGTATAAAGTATATATGAGCCATAAATGAATTTTGTGTTTAG
2721 ACTTTGTGTCCATCCCCAAGATCTCTCATTTTATATATATATATATATATATATATATATATATATATATATATATATAC
2801 ACACACACACACATACACAAATATTCCAGGATACAAAAAAAAACATTTAAAAATCCGAGACCCAGAACACTTCTGGTCCC
2881 AAGCATTTCAGATAAGGGATATCAATCTGTACTACCAATAAGGATTTCGTAATTCCCCTAACTGCAAATGTCCTCTTCAT
2961 TTGTTCTTTATGAGAAAACCCGGGTAGTGCCAGCACCTGGATACAGTATTTACACCCTGCAGACCCTAAAGATTTCAGAT
3041 TCAGTTAGCAAACCTTGATGAAGCACCTGCTGGACACTGAGGGACCCAAAGCTCAATCAGCCATAATCCCTGCTTTCAGA
3121 GTTTATATTGTACCTGCCTAATCCACCCGGCGTGACTCATTTCAACACTAAGTACTAGGGGTGTTGTCAGGAGACAAATC
3201 TGAAGTCAGGAGAGGAAAATGCAAAGGAGCCCTGCCGTGTGATGGATGTGCATTCTCACTTGGGTCTTGAAGTTCTCATT
3281 CCTACATCTCAAGCTAGCCAGGCAGTCTCCTCTCTATCAGAAGAAAGCACTGGTAATTGGCTAGACTGGCTATGTTGAAG
3361 GTAACATGAACTCTAAGATCTTGACCCAGGGCGACTTGGTTTTGCTTAAGGTGGCATCACCAATGTTCCAAATCCTTTAG
3441 GGAGATGAGGGTATCCCCACAGAAAAAGAGGAATAATAGACCAATGGATTTTCTCCTTTCACCAGTATGTTTGGAACCCT
3521 CTGATCCAATGTCTTTTGATACTGATCTCTTGTCCAAATGAGAATGTCGCTTTAGCTGAAATTCAAATGGCTGTGACAAT
3601 TTACCGAAATGATGAAGTAACCACCATTCCCACCTTTCACTGCCTAGGCTCCAAGTCTGAATACATTTTTGAAATAGGAA
3681 CTCCCTTTTGCAAAAAAGAAACCTGGGTGTCAGGGAGGTGAAGTGACTTGCCCTAGGAGCAGACAGCATGCCAAGAATGG
3761 AATTAGGCTCAGGATCCAGCCTGGGCTCACCCTGTGTGGCTCATTCCCACCCAGGAAACTGAAGATAAAAGATTTGGGAA
3841 AACACACCAAGAAAAAGGGGCAGTTTTCTTTGCCCAAGCATTTGGTGCTAGTTAGAGGCTGTTCACTCTCTCCTGCTCCT
3921 CTTCGGAGTAGAAATAAAGGCTGTGACACAAGGAAGCCAGTGGGGTGGGAGGGAGGCACCATAATCCCTCCCTAAAACCC
4001 ACAGAAGACTAACCTGATACTCTTTTGACCCAACTGCATCAACACTAAACAGCTGCAGACCCCCTGAATCTTTCACACAT
4081 GCCAAGTGAACATTCTTGATGATTTCTCTTTGTGACCGCAACCACCTGCAAACCAGAACGACTCTAGAATTTCCTTCCCC
4161 GCCCCCCTTTTTGTTTAGTTTCTAATCTCTTGTTTATGAGGTGTGGGGTTTATAAGGGACTGAATCAAATGAATGTAACA
4241 AAAAAGAAAAAAAAAACAAAAAAAAATGCCTTTTCTCAGGGCCAGTGAGTTGCAAATAATTTTTAAAGAAAAGCCTATAA
4321 TTACATCATCTCAATAAATTTTTTATAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' cguUCGGGUC--UGG--CGUUUUUc 5'
             ||:||||  ||:  ||||||| 
Target 5' agcAGTCCAGAAACTGGGCAAAAAt 3'
1026 - 1050 162.00 -15.70
2
miRNA  3' cgUUCGGGUCUGGCGUUUUUc 5'
            || :||   ::||||||| 
Target 5' ggAACTCCCTTTTGCAAAAAa 3'
3677 - 3697 151.00 -11.50
3
miRNA  3' cguucGGGUCUGGCGUUUUUc 5'
               |||| | :||||:|| 
Target 5' cttcaCCCA-AGTGCAAGAAc 3'
2276 - 2295 139.00 -12.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-129-5p :: ETV6    [ Functional MTI ]
Validation Method In situ hybridization , Microarray , qRT-PCR , Other
Conditions T24 , SW780 , HT1376 , RT4 , J82 , HU609 , HCV29
Disease bladder cancer;
Location of target site 3'UTR
Tools used in this research PicTar , TargetScan
Original Description (Extracted from the article) ... GALNT1 and SOX4 both showed significant reduction in luciferase activity on transfection with pre-miR-129 compared with GALNT1 and SOX4 3'-UTRs with mutated miR-129 target sites.// ...

- Dyrskjot, L. Ostenfeld, M. S. Bramsen, J. et al., 2009, Cancer Res.

Article - Dyrskjot, L. Ostenfeld, M. S. Bramsen, J. et al.
- Cancer Res, 2009
microRNAs (miRNA) are involved in cancer development and progression, acting as tumor suppressors or oncogenes. Here, we profiled the expression of 290 unique human miRNAs in 11 normal and 106 bladder tumor samples using spotted locked nucleic acid-based oligonucleotide microarrays. We identified several differentially expressed miRNAs between normal urothelium and cancer and between the different disease stages. miR-145 was found to be the most down-regulated in cancer compared with normal, and miR-21 was the most up-regulated in cancer. Furthermore, we identified miRNAs that significantly correlated to the presence of concomitant carcinoma in situ. We identified several miRNAs with prognostic potential for predicting disease progression (e.g., miR-129, miR-133b, and miR-518c*). We localized the expression of miR-145, miR-21, and miR-129 to urothelium by in situ hybridization. We then focused on miR-129 that exerted significant growth inhibition and induced cell death upon transfection with a miR-129 precursor in bladder carcinoma cell lines T24 and SW780 cells. Microarray analysis of T24 cells after transfection showed significant miR-129 target down-regulation (P = 0.0002) and pathway analysis indicated that targets were involved in cell death processes. By analyzing gene expression data from clinical tumor samples, we identified significant expression changes of target mRNA molecules related to the miRNA expression. Using luciferase assays, we documented a direct link between miR-129 and the two putative targets GALNT1 and SOX4. The findings reported here indicate that several miRNAs are differentially regulated in bladder cancer and may form a basis for clinical development of new biomarkers for bladder cancer.
LinkOut: [PMID: 19487295]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
16 hsa-miR-129-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001953 CAMTA1 calmodulin binding transcription activator 1 2 1
MIRT001954 EIF2C3 eukaryotic translation initiation factor 2C, 3 2 1
MIRT004043 SOX4 SRY (sex determining region Y)-box 4 5 3
MIRT004049 GALNT1 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) 3 2
MIRT004050 ETV6 ets variant 6 3 1
MIRT004051 BMPR2 bone morphogenetic protein receptor, type II (serine/threonine kinase) 3 1
MIRT005412 NOTCH1 Notch homolog 1, translocation-associated (Drosophila) 3 1
MIRT006660 UBE2F ubiquitin-conjugating enzyme E2F (putative) 1 1
MIRT007376 FMR1 fragile X mental retardation 1 1 1
MIRT026018 PDS5A PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae) 1 1
MIRT026019 TNPO1 transportin 1 1 1
MIRT026020 TP53INP1 tumor protein p53 inducible nuclear protein 1 1 1
MIRT026021 FNDC3B fibronectin type III domain containing 3B 1 1
MIRT026022 ZFP91 zinc finger protein 91 homolog (mouse) 1 1
MIRT048045 NSMF nasal embryonic LHRH factor 1 1
MIRT048046 HIST1H2BJ histone cluster 1, H2bj 1 1