Accession ID: MIRT004071 [miRNA, hsa-miR-125a-5p :: TP53, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-125a LinkOut: [miRBase ]
Description Homo sapiens miR-125a stem-loop
Comment This miRNA sequence is predicted based on homology to a verified miRNA from mouse .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-125a-5p
Mature Sequence 15| UCCCUGAGACCCUUUAACCUGUGA |38
Evidence Experimental
Experiments Cloned
Putative hsa-miR-125a-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol TP53 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms FLJ92943, LFS1, TRP53, p53
Description tumor protein p53
Transcript NM_001126114   LinkOut: [ RefSeq ]
Other Transcripts NM_001126112, NM_000546, NM_001126113, NM_001126115, NM_001126116, NM_001126117   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04010    MAPK signaling pathway - Homo sapiens (human)
hsa04110    Cell cycle - Homo sapiens (human)
hsa04115    p53 signaling pathway - Homo sapiens (human)
hsa04210    Apoptosis - Homo sapiens (human)
hsa04310    Wnt signaling pathway - Homo sapiens (human)
hsa04722    Neurotrophin signaling pathway - Homo sapiens (human)
hsa05014    Amyotrophic lateral sclerosis (ALS) - Homo sapiens (human)
hsa05016    Huntington's disease - Homo sapiens (human)
hsa05200    Pathways in cancer - Homo sapiens (human)
hsa05210    Colorectal cancer - Homo sapiens (human)
hsa05212    Pancreatic cancer - Homo sapiens (human)
hsa05213    Endometrial cancer - Homo sapiens (human)
hsa05214    Glioma - Homo sapiens (human)
hsa05215    Prostate cancer - Homo sapiens (human)
hsa05216    Thyroid cancer - Homo sapiens (human)
hsa05217    Basal cell carcinoma - Homo sapiens (human)
hsa05218    Melanoma - Homo sapiens (human)
hsa05219    Bladder cancer - Homo sapiens (human)
hsa05220    Chronic myeloid leukemia - Homo sapiens (human)
hsa05222    Small cell lung cancer - Homo sapiens (human)
hsa05223    Non-small cell lung cancer - Homo sapiens (human)
Putative miRNA Targets on TP53 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of TP53
(miRNA target sites are highlighted)		 
>TP53|NM_001126114|3'UTR
   1 TAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATACAGATGCTACTTGACTTACGATGGTGTTACTTCCTGATAA
  81 ACTCGTCGTAAGTTGAAAATATTATCCGTGGGCGTGAGCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTC
 161 AAGGATGCCCAGGCTGGGAAGGAGCCAGGGGGGAGCAGGGCTCACTCCAGCCACCTGAAGTCCAAAAAGGGTCAGTCTAC
 241 CTCCCGCCATAAAAAACTCATGTTCAAGACAGAAGGGCCTGACTCAGACTGACATTCTCCACTTCTTGTTCCCCACTGAC
 321 AGCCTCCCACCCCCATCTCTCCCTCCCCTGCCATTTTGGGTTTTGGGTCTTTGAACCCTTGCTTGCAATAGGTGTGCGTC
 401 AGAAGCACCCAGGACTTCCATTTGCTTTGTCCCGGGGCTCCACTGAACAAGTTGGCCTGCACTGGTGTTTTGTTGTGGGG
 481 AGGAGGATGGGGAGTAGGACATACCAGCTTAGATTTTAAGGTTTTTACTGTGAGGGATGTTTGGGAGATGTAAGAAATGT
 561 TCTTGCAGTTAAGGGTTAGTTTACAATCAGCCACATTCTAGGTAGGGGCCCACTTCACCGTACTAACCAGGGAAGCTGTC
 641 CCTCACTGTTGAATTTTCTCTAACTTCAAGGCCCATATCTGTGAAATGCTGGCATTTGCACCTACCTCACAGAGTGCATT
 721 GTGAGGGTTAATGAAATAATGTACATCTGGCCTTGAAACCACCTTTTATTACATGGGGTCTAGAACTTGACCCCCTTGAG
 801 GGTGCTTGTTCCCTCTCCCTGTTGGTCGGTGGGTTGGTAGTTTCTACAGTTGGGCAGCTGGTTAGGTAGAGGGAGTTGTC
 881 AAGTCTCTGCTGGCCCAGCCAAACCCTGTCTGACAACCTCTTGGTGAACCTTAGTACCTAAAAGGAAATCTCACCCCATC
 961 CCACACCCTGGAGGATTTCATCTCTTGTATATGATGATCTGGATCCACCAAGACTTGTTTTATGCTCAGGGTCAATTTCT
1041 TTTTTCTTTTTTTTTTTTTTTTTTCTTTTTCTTTGAGACTGGGTCTCGCTTTGTTGCCCAGGCTGGAGTGGAGTGGCGTG
1121 ATCTTGGCTTACTGCAGCCTTTGCCTCCCCGGCTCGAGCAGTCCTGCCTCAGCCTCCGGAGTAGCTGGGACCACAGGTTC
1201 ATGCCACCATGGCCAGCCAACTTTTGCATGTTTTGTAGAGATGGGGTCTCACAGTGTTGCCCAGGCTGGTCTCAAACTCC
1281 TGGGCTCAGGCGATCCACCTGTCTCAGCCTCCCAGAGTGCTGGGATTACAATTGTGAGCCACCACGTCCAGCTGGAAGGG
1361 TCAACATCTTTTACATTCTGCAAGCACATCTGCATTTTCACCCCACCCTTCCCCTCCTTCTCCCTTTTTATATCCCATTT
1441 TTATATCGATCTCTTATTTTACAATAAAACTTTGCTGCCACCTGTGTGTCTGAGGGGTG
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' agUGUCCAAUUUCCCAGAGUCCCu 5'
            ||  |||  | |  ||||||| 
Target 5' agACTTGTTTTATG--CTCAGGGt 3'
 		1011 - 1032 150.00 -15.00
2 
miRNA  3' agUGUCCA-A-UUU-----CCCAGAGUCCcu 5'
            :| ||| | |||     ||| ||||||  
Target 5' agGCTGGTCTCAAACTCCTGGG-CTCAGGcg 3'
 		1263 - 1292 133.00 -16.30
3 
miRNA  3' aguguccaauuuCCCAGAGUCCCu 5'
                      | |||| |||| 
Target 5' gctgccacctgtGTGTCTGAGGGg 3'
 		1474 - 1497 120.00 -17.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-125a-5p :: TP53    [ Functional MTI ]
Validation Method Luciferase reporter assay , Northern blot , qRT-PCR , Western blot
Conditions HEK293T, HepG2, MCF-7
Location of target site 3'UTR
Original Description (Extracted from the article) ... Here, we show that an additional isomer of miRNA-125 (miRNA-125a) translationally arrests mRNA of the p53 tumor suppressor gene. The basis of this activity is the high degree of sequence homology between the seed sequence of miR-125a and the 30-UTR of p53. Our findings add miRNA-125a to the growing list of miRNA with oncogenic targets.// ...

- Zhang, Y. Gao, J. S. Tang, X. Tucker, L. D. et al., 2009, FEBS Lett.
miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1 
miRNA  3' agUGUCCAAUUUCCCAGAGUCCCu 5'
            ||  |||  | |  ||||||| 
Target 5' -gACUUGUUUUAUG--CUCAGGG- 3'
 		1 - 20
Article - Zhang, Y. Gao, J. S. Tang, X. Tucker, L. D. et al.
- FEBS Lett, 2009
MicroRNA (miRNA) are a class of non-coding RNA that suppress gene expression by degradation or translational inhibition of target RNA. Several miRNA have been shown to target oncogenes and recently miRNA-125b was shown to translationally and transcriptionally inhibit the p53 gene. Here, we show that an additional isomer of miRNA-125 (miRNA-125a) translationally arrests mRNA of the p53 tumor suppressor gene. The basis of this activity is the high degree of sequence homology between the seed sequence of miR-125a and the 3'-UTR of p53. Our findings add miRNA-125a to the growing list of miRNA with oncogenic targets.
LinkOut: [PMID: 19818772]