Accession ID: MIRT004103 [miRNA, hsa-miR-29a-3p :: PIK3R1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-29aLinkOut: [miRBase ]
Synonyms MIRN29, MIRN29A, hsa-mir-29, hsa-mir-29a, miRNA29A, MIR29A
Description Homo sapiens miR-29a stem-loop
Comment miR-29a was previously know as miR-29 here and in .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-29a-3p
Mature Sequence 42| UAGCACCAUCUGAAAUCGGUUA |63
Evidence Experimental
Experiments Northern
Putative hsa-miR-29a-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol PIK3R1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms GRB1, p85, p85-ALPHA
Description phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Transcript NM_1815    LinkOut: [ RefSeq ]
Other Transcripts NM_1815 , NM_1815   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on PIK3R1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of PIK3R1
(miRNA target sites are highlighted)
>PIK3R1|NM_1815|3'UTR
   1 TGAAGCGCTTACTCTTTGATCCTTCTCCTGAAGTTCAGCCACCCTGAGGCCTCTGGAAAGCAAAGGGCTCCTCTCCAGTC
  81 TGATCTGTGAATTGAGCTGCAGAAACGAAGCCATCTTTCTTTGGATGGGACTAGAGCTTTCTTTCACAAAAAAGAAGTAG
 161 GGGAAGACATGCAGCCTAAGGCTGTATGATGACCACACGTTCCTAAGCTGGAGTGCTTATCCCTTCTTTTTCTTTTTTTC
 241 TTTGGTTTAATTTAAAGCCACAACCACATACAACACAAAGAGAAAAAGAAATGCAAAAATCTCTGCGTGCAGGGACAAAG
 321 AGGCCTTTAACCATGGTGCTTGTTAATGCTTTCTGAAGCTTTACCAGCTGAAAGTTGGGACTCTGGAGAGCGGAGGAGAG
 401 AGAGGCAGAAGAACCCTGGCCTGAGAAGGTTTGGTCCAGCCTGGTTTAGCCTGGATGTTGCTGTGCACGGTGGACCCAGA
 481 CACATCGCACTGTGGATTATTTCATTTTGTAACAAATGAACGATATGTAGCAGAAAGGCACGTCCACTCACAAGGGACGC
 561 TTTGGGAGAATGTCAGTTCATGTATGTTCAGAAGAAATTCTGTCATAGAAAGTGCCAGAAAGTGTTTAACTTGTCAAAAA
 641 ACAAAAACCCAGCAACAGAAAAATGGAGTTTGGAAAACAGGACTTAAAATGACATTCAGTATATAAAATATGTACATAAT
 721 ATTGGATGACTAACTATCAAATAGATGGATTTGTATCAATACCAAATAGCTTCTGTTTTGTTTTGCTGAAGGCTAAATTC
 801 ACAGCGCTATGCAATTCTTAATTTTCATTAAGTTGTTATTTCAGTTTTAAATGTACCTTCAGAATAAGCTTCCCCACCCC
 881 AGTTTTTGTTGCTTGAAAATATTGTTGTCCCGGATTTTTGTTAATATTCATTTTTGTTATCCTTTTTTAAAAGTAAATGT
 961 ACAGGATGCCAGTAAAAAAAAAAAATGGCTTCAGAATTAAAACTATGAAATATTTTACAGTTTTTCTTGTACAGAGTACT
1041 TGGCTGTTAGCCCAAGGTTAAAAAGTTCATAACAGATTTTTTTTGGACTGTTTTGTTGGGCAGTGCCTGATAAGCTTCAA
1121 AGCTGCTTTATTCAATAAAAAAAAGAAATGAAAAAGATATATGAATATGACAAAGTATTGCTGAGTCCAACAATGTTGTT
1201 TTAAGACTCTTAAAATACGGTACCTGGCAATGTTTATTTCATAAAGAATTGTGAACTTCTTGAATCTAGGGAGGGGGAAT
1281 GTAGTGAAGGGATGTATCAAGTGGGGTGGTGGGAGGGGGAGGCAAGGTTATATGCACTTTCTCATGATTTACAGAGAAGT
1361 GAATAACTGCAAAGTGAAGTTGCTTCTTCTACTTCAGTCTTCTCTCACTTTGATTTGCTAGTTGTTATCAATTAATGACA
1441 ATTACAAACCTACTGTATCTCTAATACAGTGTGACTGGTCAGGTATTTCAGTTCTTAGGAAGGAAGTGCCAAGTTTGTTT
1521 TTGGGTTCCTGGAACAGCGCTCACCTTTGTTTAGAACACTGGTTTAAAGGGATAATCATCTCTGTCACATTAGACTATCC
1601 ATCATGACCAGCAAATACTCATTTTAGGAAAAAAAAAAGCATGATCTGAAAAATACTTTTGGTGGTATGTTGGTTACCCT
1681 CCTAGCTTTCCATTTGGTTTAGAACATAAAGCAAATAGACACAGTCATACTGTCACTGCTCTGGACTGTGTGGAGCTCGC
1761 TAAAGTCATGGTCATTGCAGGAATCCAAGTGGCAGTCCTTCTCATTCATTCTAATCATTGTATGTGCTTCACTACGGGGG
1841 GGAGAAGGAAACGTTAGCATCATGTTTCCCATTTAGGGCAGGAGTGAGAGGTCTCTCTTCCTGATTTAGATATGCAAAAG
1921 CTGGTATGTTCAGTAGGAACTGTACATGTGTTGGGAGGCATAAAGACTAATTAGCAACCATAATATGGTCACTACCCTAA
2001 TAGACTAAATGAAATCTTGCAATTTCAAATTACTCTTTCTCCATATTAGATTTACCCACAGCTATATTTCTGTTTAAGTA
2081 CTAGGGTGAGGGTTTTCTGTTACTTTGTTTTTTAATGTTGTTCCTTTTGAAAGAATCAGTCTTGCAGCTGAGTGAAAAAT
2161 CTGTGGAATGTATTATTTGTCCTCTTTACATGAAACTACTCATACTTAAGCAAAAGTCAGTCTTATAGCAAGACTGTTAG
2241 CCCTCAAACTTGACTCTACTGATCTGACCATTTCCCTCTCATCGCCAGACAACTGACGATTTCCCTGGTTTTAGTCTGCG
2321 TCTCTGCTTTAAAGTTATTGTGATATCCTTCTAGATCATACACAAGTCTAACAGTTAATTAGTTAACAGTTTTTAAACTA
2401 GGTTTGTGGGTATTTTTTTGGTAGCACATGTATGCTATTACATACAAATTTTTATTTCTAAAATATAAGATCTGAGATTG
2481 AATATTTTCATTAAAAGCTACAGTTTTGTGAATCTTTGTGCTTCAACATTCTTTGCAAGATGATACGGTATTTAGGCATT
2561 TGCCTTATTTTTGCATCTCACAAACATAAGTGCAATAGATCTTTTCATTGAACAGCAAAGTAGGATTCATCATTCCATAT
2641 GACTTGAGTTACACCAGACCTGTTCTGCCCAATGCCTTTTTGATTACAGTGTAGCTTGCCCACCGCATTTGTCGTTTTAG
2721 ATACTTTGCTAGCCGGCCACTTTGGATTTCATCAGACAGTCCTAACAATATTGTCTGAACGGCTGAATATGAATAGATAC
2801 AGCAGAGGCACTCCTGATATATGATTTTTATCCATGCGTCAGTTTTTCCCACCCAGTGTAGCATCCTAAAGATAAAGCCA
2881 GAAGCTAAGCTGCAGTGAGGCTGTGATTGGGCGTAGAAGTGGGAGCATTGGGACCTCACATTACACACACGAGAGATCAT
2961 AACCATGTGAAAAGGCAAAAAGCATGTGTTTGCAACATCTGATAACTTCATGGCCTTTGATAAATGTATATATGTATATG
3041 TGCATGGACTGTGTTTCCAGTACACCTTTCAGCCAAAACAGATCCACAGTAGTTGTTGAGTTCAAGTACATAAAGTACAT
3121 AACAAGCGAACGTCTAGTACAATTCTTACTTATGTGTATGGGATTTTTCCCTTTGAGGTTGCTTTGTTTTGTCTTACAAA
3201 GGTGAAAATTGTTTGTAAGTGAAGTGAGAAGTTCATATTTCTTTGGCTTTTTTGTGTTTTTAAAAGTTACTCCTTTTAGG
3281 GAGCTGGTCTGATGACTTGCTTAGCTTGGAAATCCTTGTTTTCAGTGTGTCGAGTCAAAATGTGTTTATGTGAGCTGTCA
3361 CTGTGGGGAACCAATTGCTTTGTCATATAGCTGGTTATGAACTAGTAACATGTTTGGGAAGTCCTACTGATGTTCCTTTG
3441 GAAGAAAAAATCTGCTGGTTTTAACAACTGTGCTTTTGCTATGTATGGTATCCAAGTTAGTTGAAACGCAGACACTGAGA
3521 TCTGTTTGAGTTTAGGGTCATTTTTAGAAAGGGGCAGTTTAAAGCACAATGTCTCACATGGGACAAAGTTCCAAAATGCC
3601 AAATTCTTATTTTTTAAAAAGCTAGTTCTATAAAATACTGGTATTATGGGTGGGGAGGAAATAGAATTGAGTCAATTGGA
3681 AAGACTATCCAACTTAACATGAAACTTGTCACCATGAGATAGCATTAGCTGCCCAGGATGCTGCTATATATATATATATA
3761 TATATATATGTGTGTGTGTGTGTGTGTGTGTGTGTATATATATATATATATATATATATATATATATATATATATGTGTG
3841 TGTATATATATATATATGTGTATATATATATGTATATACATATATGTATATATATGCACATATATATATGTATTTAAAAA
3921 AATCAAAACAAAAAAAAACTCATTTATACCTGTGTATTTTTTAAAGCTACAATCTGTTCAATGTTTTTAAAAATCTGTTT
4001 ATATGACATTGTTAAAATAAAGTTGGTCTTTTGACGAGAGGGAGGATGTCACGGTCAGTTGTAACTTTGCCTTCACAAGG
4081 CAACTGGGGTGGGGGGTGGGGGTAGTGTGCCTCCTTGACATTTCGTTCAAGTTATAGATTCAATGGAGCTATGTCTTGTT
4161 TTAAGTTGCTTTAATGCATTGTATTAGATCTTCAAACAGAATAAAGGTTGTTTTGAAACTGAAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' auUGGCUAAAGU-CUACCACGAu 5'
            :||  ||| |  |||||||| 
Target 5' agGCC--TTTAACCATGGTGCTt 3'
321 - 341 152.00 -12.40
2
miRNA  3' auuGGCUAAA----GUCUACCACGAu 5'
             |:|:|||    ::|||| |||| 
Target 5' agcCTGGTTTAGCCTGGATGTTGCTg 3'
438 - 463 133.00 -14.80
3
miRNA  3' auUGGCUAAAGUCUACCACGAu 5'
            |: || |||| |||| ||| 
Target 5' ttATAGA-TTCA-ATGGAGCTa 3'
4132 - 4151 130.00 -10.30
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-29a-3p :: PIK3R1    [ Functional MTI ]
Validation Method Other
Article - Park, S. Y. Lee, J. H. Ha, M. Nam, J. W. et al.
- Nat Struct Mol Biol, 2009
The tumor suppressor p53 is central to many cellular stress responses. Although numerous protein factors that control p53 have been identified, the role of microRNAs (miRNAs) in regulating p53 remains unexplored. In a screen for miRNAs that modulate p53 activity, we find that miR-29 family members (miR-29a, miR-29b and miR-29c) upregulate p53 levels and induce apoptosis in a p53-dependent manner. We further find that miR-29 family members directly suppress p85 alpha (the regulatory subunit of PI3 kinase) and CDC42 (a Rho family GTPase), both of which negatively regulate p53. Our findings provide new insights into the role of miRNAs in the p53 pathway.
LinkOut: [PMID: 19079265]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-29a-3p :: PIK3R1    [ Functional MTI ]
Validation Method Luciferase reporter assay , Northern blot , qRT-PCR , Western blot , Other
Conditions Hep G2
Disease diabetes mellitus type 2;
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... miR-29a directly targets the PI3Kp85α 3'UTR ...

- Pandey, A. K. Verma, G. Vig, S. Srivastava, et al., 2011, Molecular and cellular endocrinology.

Article - Pandey, A. K. Verma, G. Vig, S. Srivastava, et al.
- Molecular and cellular endocrinology, 2011
MicroRNAs comprise a class of small ( approximately 22 nucleotide) non-coding RNA species and they bind to their complementary sequence on the 3'UTR of target genes and cause translational repression. In the present study, we report that miR-29a levels are significantly elevated in the diabetic db/db mice liver. Further, we report the effects of such elevation on insulin action in HepG2 cells. Overexpression of miR-29a narrowed down insulin mediated Akt phosphorylation without altering the total Akt levels presumably due to another upstream mediator being directly targeted by miR-29a. This hunt led us to the discovery that the p85alpha subunit of PI3K (phosphoionositide-3-kinase), the upstream molecule in the insulin signaling cascade harbors the miR-29a binding site on its 3'UTR and a marked inhibition of PI3Kp85alpha was observed by this microRNA. This was consequently accompanied by attenuation of insulin inhibition of PEPCK gene expression. All these events could be significantly prevented in the presence of the miR-29a inhibitor. Our results, for the first time, show the effect of miR-29a in counteracting insulin action on PEPCK gene expression by primarily targeting PI3K and abrogating downstream insulin signaling in HepG2 cells.
LinkOut: [PMID: 20943204]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
76 hsa-miR-29a-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000682 CDK6 cyclin-dependent kinase 6 4 2
MIRT001196 RAN RAN, member RAS oncogene family 3 1
MIRT001766 BACE1 beta-site APP-cleaving enzyme 1 3 1
MIRT003021 DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha 5 4
MIRT003027 DNMT3B DNA (cytosine-5-)-methyltransferase 3 beta 4 3
MIRT003220 COL4A2 collagen, type IV, alpha 2 4 2
MIRT003221 COL4A1 collagen, type IV, alpha 1 4 1
MIRT003286 MCL1 myeloid cell leukemia sequence 1 (BCL2-related) 5 5
MIRT003291 BCL2 B-cell CLL/lymphoma 2 4 1
MIRT003343 CD276 CD276 molecule 3 2
MIRT003607 SFRP2 secreted frizzled-related protein 2 4 1
MIRT003609 DKK1 dickkopf homolog 1 (Xenopus laevis) 4 1
MIRT003735 S100B S100 calcium binding protein B 3 1
MIRT003737 IMPDH1 IMP (inosine 5'-monophosphate) dehydrogenase 1 2 1
MIRT004021 GLUL glutamate-ammonia ligase 4 1
MIRT004044 PPM1D protein phosphatase, Mg2+/Mn2+ dependent, 1D 5 1
MIRT004103 PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) 4 2
MIRT004420 TET1 tet oncogene 1 2 1
MIRT004564 KREMEN2 kringle containing transmembrane protein 2 4 1
MIRT004630 ZFP36 zinc finger protein 36, C3H type, homolog (mouse) 1 2
MIRT005521 FGG fibrinogen gamma chain 2 1
MIRT005531 FGA fibrinogen alpha chain 2 1
MIRT005532 FGB fibrinogen beta chain 2 1
MIRT005584 LPL lipoprotein lipase 4 1
MIRT005591 CPEB3 cytoplasmic polyadenylation element binding protein 3 3 1
MIRT005592 CPEB4 cytoplasmic polyadenylation element binding protein 4 3 1
MIRT005668 ADAMTS9 ADAM metallopeptidase with thrombospondin type 1 motif, 9 4 1
MIRT005675 ITGA11 integrin, alpha 11 4 1
MIRT005906 DAG1 dystroglycan 1 (dystrophin-associated glycoprotein 1) 1 1
MIRT005907 DIABLO diablo homolog (Drosophila) 1 1
MIRT005908 RET ret proto-oncogene 1 1
MIRT005909 TRIM63 tripartite motif-containing 63 1 1
MIRT005910 TGFB3 transforming growth factor, beta 3 1 1
MIRT006111 SAPCD2 chromosome 9 open reading frame 140 3 1
MIRT006135 MYCN v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) 2 2
MIRT006175 SPARC secreted protein, acidic, cysteine-rich (osteonectin) 5 3
MIRT006182 SERPINB9 serpin peptidase inhibitor, clade B (ovalbumin), member 9 4 1
MIRT006250 NASP nuclear autoantigenic sperm protein (histone-binding) 2 2
MIRT006379 DICER1 dicer 1, ribonuclease type III 1 1
MIRT006380 TNFAIP3 tumor necrosis factor, alpha-induced protein 3 1 1
MIRT006485 PPP1R13B protein phosphatase 1, regulatory (inhibitor) subunit 13B 2 1
MIRT006490 CDC42 cell division cycle 42 (GTP binding protein, 25kDa) 3 1
MIRT006549 NAV3 neuron navigator 3 1 1
MIRT006551 PXDN peroxidasin homolog (Drosophila) 2 1
MIRT006552 BCL7A B-cell CLL/lymphoma 7A 1 1
MIRT006553 ITIH5 inter-alpha (globulin) inhibitor H5 1 1
MIRT006873 C1QTNF6 C1q and tumor necrosis factor related protein 6 1 1
MIRT007028 PTEN phosphatase and tensin homolog 1 2
MIRT007197 ABL1 c-abl oncogene 1, receptor tyrosine kinase 1 1
MIRT028683 COL3A1 collagen, type III, alpha 1 1 1
MIRT035539 HBP1 HMG-box transcription factor 1 1 1
MIRT049964 ZFP91 zinc finger protein 91 homolog (mouse) 1 1
MIRT049965 CYTB cytochrome b 1 1
MIRT049966 CCDC14 coiled-coil domain containing 14 1 1
MIRT049967 AGO1 eukaryotic translation initiation factor 2C, 1 1 1
MIRT049968 WBSCR16 Williams-Beuren syndrome chromosome region 16 1 1
MIRT049969 EIF3E eukaryotic translation initiation factor 3, subunit E 1 1
MIRT049970 POLD3 polymerase (DNA-directed), delta 3, accessory subunit 1 1
MIRT049971 RPS6KA3 ribosomal protein S6 kinase, 90kDa, polypeptide 3 1 1
MIRT049972 FAM133A family with sequence similarity 133, member A 1 1
MIRT049973 BCAP31 B-cell receptor-associated protein 31 1 1
MIRT049974 OAZ1 ornithine decarboxylase antizyme 1 1 1
MIRT049975 RRAGC Ras-related GTP binding C 1 1
MIRT049976 GAREML family with sequence similarity 59, member B 1 1
MIRT049977 RPS4X ribosomal protein S4, X-linked 1 1
MIRT049978 SUV420H2 suppressor of variegation 4-20 homolog 2 (Drosophila) 1 1
MIRT049979 CCT4 chaperonin containing TCP1, subunit 4 (delta) 1 1
MIRT049980 SARS seryl-tRNA synthetase 1 1
MIRT049981 PPT1 palmitoyl-protein thioesterase 1 1 1
MIRT049982 CRKL v-crk sarcoma virus CT10 oncogene homolog (avian)-like 1 1
MIRT049983 SP2 Sp2 transcription factor 1 1
MIRT049984 HUWE1 HECT, UBA and WWE domain containing 1 1 1
MIRT049985 SYNCRIP synaptotagmin binding, cytoplasmic RNA interacting protein 1 1
MIRT049986 HNRNPM heterogeneous nuclear ribonucleoprotein M 1 1
MIRT049987 FAM109B family with sequence similarity 109, member B 1 1
MIRT049988 PAN2 PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) 1 1