Accession ID: MIRT004118 [miRNA, hsa-miR-483-3p :: SMAD4, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-483LinkOut: [miRBase ]
Synonyms MIRN483, hsa-mir-483, MIR483
Description Homo sapiens miR-483 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-483-3p
Mature Sequence 48| UCACUCCUCUCCUCCCGUCUU |68
Evidence Experimental
Experiments Cloned
Putative hsa-miR-483-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol SMAD4 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms DPC4, JIP, MADH4
Description SMAD family member 4
Transcript NM_0053    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on SMAD4 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of SMAD4
(miRNA target sites are highlighted)
>SMAD4|NM_0053|3'UTR
   1 CCTTTAGACTGAGGTCTTTTACCGTTGGGGCCCTTAACCTTATCAGGATGGTGGACTACAAAATACAATCCTGTTTATAA
  81 TCTGAAGATATATTTCACTTTTGTTCTGCTTTATCTTTTCATAAAGGGTTGAAAATGTGTTTGCTGCCTTGCTCCTAGCA
 161 GACAGAAACTGGATTAAAACAATTTTTTTTTTCCTCTTCAGAACTTGTCAGGCATGGCTCAGAGCTTGAAGATTAGGAGA
 241 AACACATTCTTATTAATTCTTCACCTGTTATGTATGAAGGAATCATTCCAGTGCTAGAAAATTTAGCCCTTTAAAACGTC
 321 TTAGAGCCTTTTATCTGCAGAACATCGATATGTATATCATTCTACAGAATAATCCAGTATTGCTGATTTTAAAGGCAGAG
 401 AAGTTCTCAAAGTTAATTCACCTATGTTATTTTGTGTACAAGTTGTTATTGTTGAACATACTTCAAAAATAATGTGCCAT
 481 GTGGGTGAGTTAATTTTACCAAGAGTAACTTTACTCTGTGTTTAAAAAGTAAGTTAATAATGTATTGTAATCTTTCATCC
 561 AAAATATTTTTTGCAAGTTATATTAGTGAAGATGGTTTCAATTCAGATTGTCTTGCAACTTCAGTTTTATTTTTGCCAAG
 641 GCAAAAAACTCTTAATCTGTGTGTATATTGAGAATCCCTTAAAATTACCAGACAAAAAAATTTAAAATTACGTTTGTTAT
 721 TCCTAGTGGATGACTGTTGATGAAGTATACTTTTCCCCTGTTAAACAGTAGTTGTATTCTTCTGTATTTCTAGGCACAAG
 801 GTTGGTTGCTAAGAAGCCTATAAGAGGAATTTCTTTTCCTTCATTCATAGGGAAAGGTTTTGTATTTTTTAAAACACTAA
 881 AAGCAGCGTCACTCTACCTAATGTCTCACTGTTCTGCAAAGGTGGCAATGCTTAAACTAAATAATGAATAAACTGAATAT
 961 TTTGGAAACTGCTAAATTCTATGTTAAATACTGTGCAGAATAATGGAAACATTACAGTTCATAATAGGTAGTTTGGATAT
1041 TTTTGTACTTGATTTGATGTGACTTTTTTTGGTATAATGTTTAAATCATGTATGTTATGATATTGTTTAAAATTCAGTTT
1121 TTGTATCTTGGGGCAAGACTGCAAACTTTTTTATATCTTTTGGTTATTCTAAGCCCTTTGCCATCAATGATCATATCAAT
1201 TGGCAGTGACTTTGTATAGAGAATTTAAGTAGAAAAGTTGCAGATGTATTGACTGTACCACAGACACAATATGTATGCTT
1281 TTTACCTAGCTGGTAGCATAAATAAAACTGAATCTCAACATACAAAGTTGAATTCTAGGTTTGATTTTTAAGATTTTTTT
1361 TTTCTTTTGCACTTTTGAGTCCAATCTCAGTGATGAGGTACCTTCTACTAAATGACAGGCAACAGCCAGTTCTATTGGGC
1441 AGCTTTGTTTTTTTCCCTCACACTCTACCGGGACTTCCCCATGGACATTGTGTATCATGTGTAGAGTTGGTTTTTTTTTT
1521 TTTTAATTTTTATTTTACTATAGCAGAAATAGACCTGATTATCTACAAGATGATAAATAGATTGTCTACAGGATAAATAG
1601 TATGAAATAAAATCAAGGATTATCTTTCAGATGTGTTTACTTTTGCCTGGAGAACTTTTAGCTATAGAAACACTTGTGTG
1681 ATGATAGTCCTCCTTATATCACCTGGAATGAACACAGCTTCTACTGCCTTGCTCAGAAGGTCTTTTAAATAGACCATCCT
1761 AGAAACCACTGAGTTTGCTTATTTCTGTGATTTAAACATAGATCTTGATCCAAGCTACATGACTTTTGTCTTTAAATAAC
1841 TTATCTACCACCTCATTTGTACTCTTGATTACTTACAAATTCTTTCAGTAAACACCTAATTTTCTTCTGTAAAAGTTTGG
1921 TGATTTAAGTTTTATTGGCAGTTTTATAAAAAGACATCTTCTCTAGAAATTGCTAACTTTAGGTCCATTTTACTGTGAAT
2001 GAGGAATAGGAGTGAGTTTTAGAATAACAGATTTTTAAAAATCCAGATGATTTGATTAAAACCTTAATCATACATTGACA
2081 TAATTCATTGCTTCTTTTTTTTGAGATATGGAGTCTTGCTGTGTTGCCCAGGCAGGAGTGCAGTGGTATGATCTCAGCTC
2161 ACTGCAACCTCTGCCTCCCGGGTTCAACTGATTCTCCTGCCTCAGCCTCCCTGGTAGCTAGGATTACAGGTGCCCGCCAC
2241 CATGCCTGGCTAACTTTTGTAGTTTTAGTAGAGACGGGGTTTTGCCTGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCA
2321 AGTGATCCATCCACCTTGGCCTCCCAAAGTGCTGGGATTACGGGCGTGAGCCACTGTCCCTGGCCTCATTGTTCCCTTTT
2401 CTACTTTAAGGAAAGTTTTCATGTTTAATCATCTGGGGAAAGTATGTGAAAAATATTTGTTAAGAAGTATCTCTTTGGAG
2481 CCAAGCCACCTGTCTTGGTTTCTTTCTACTAAGAGCCATAAAGTATAGAAATACTTCTAGTTGTTAAGTGCTTATATTTG
2561 TACCTAGATTTAGTCACACGCTTTTGAGAAAACATCTAGTATGTTATGATCAGCTATTCCTGAGAGCTTGGTTGTTAATC
2641 TATATTTCTATTTCTTAGTGGTAGTCATCTTTGATGAATAAGACTAAAGATTCTCACAGGTTTAAAATTTTATGTCTACT
2721 TTAAGGGTAAAATTATGAGGTTATGGTTCTGGGTGGGTTTTCTCTAGCTAATTCATATCTCAAAGAGTCTCAAAATGTTG
2801 AATTTCAGTGCAAGCTGAATGAGAGATGAGCCATGTACACCCACCGTAAGACCTCATTCCATGTTTGTCCAGTGCCTTTC
2881 AGTGCATTATCAAAGGGAATCCTTCATGGTGTTGCCTTTATTTTCCGGGGAGTAGATCGTGGGATATAGTCTATCTCATT
2961 TTTAATAGTTTACCGCCCCTGGTATACAAAGATAATGACAATAAATCACTGCCATATAACCTTGCTTTTTCCAGAAACAT
3041 GGCTGTTTTGTATTGCTGTAACCACTAAATAGGTTGCCTATACCATTCCTCCTGTGAACAGTGCAGATTTACAGGTTGCA
3121 TGGTCTGGCTTAAGGAGAGCCATACTTGAGACATGTGAGTAAACTGAACTCATATTAGCTGTGCTGCATTTCAGACTTAA
3201 AATCCATTTTTGTGGGGCAGGGTGTGGTGTGTAAAGGGGGGTGTTTGTAATACAAGTTGAAGGCAAAATAAAATGTCCTG
3281 TCTCCCAGATGATATACATCTTATTATTTTTAAAGTTTATTGCTAATTGTAGGAAGGTGAGTTGCAGGTATCTTTGACTA
3361 TGGTCATCTGGGGAAGGAAAATTTTACATTTTACTATTAATGCTCCTTAAGTGTCTATGGAGGTTAAAGAATAAAATGGT
3441 AAATGTTTCTGTGCCTGGTTTGATGGTAACTGGTTAATAGTTACTCACCATTTTATGCAGAGTCACATTAGTTCACACCC
3521 TTTCTGAGAGCCTTTTGGGAGAAGCAGTTTTATTCTCTGAGTGGAACAGAGTTCTTTTTGTTGATAATTTCTAGTTTGCT
3601 CCCTTCGTTATTGCCAACTTTACTGGCATTTTATTTAATGATAGCAGATTGGGAAAATGGCAAATTTAGGTTACGGAGGT
3681 AAATGAGTATATGAAAGCAATTACCTCTAAAGCCAGTTAACAATTATTTTGTAGGTGGGGTACACTCAGCTTAAAGTAAT
3761 GCATTTTTTTTTCCCGTAAAGGCAGAATCCATCTTGTTGCAGATAGCTATCTAAATAATCTCATATCCTCTTTTGCAAAG
3841 ACTACAGAGAATAGGCTATGACAATCTTGTTCAAGCCTTTCCATTTTTTTCCCTGATAACTAAGTAATTTCTTTGAACAT
3921 ACCAAGAAGTATGTAAAAAGTCCATGGCCTTATTCATCCACAAAGTGGCATCCTAGGCCCAGCCTTATCCCTAGCAGTTG
4001 TCCCAGTGCTGCTAGGTTGCTTATCTTGTTTATCTGGAATCACTGTGGAGTGAAATTTTCCACATCATCCAGAATTGCCT
4081 TATTTAAGAAGTAAAACGTTTTAATTTTTAGCCTTTTTTTGGTGGAGTTATTTAATATGTATATCAGAGGATATACTAGA
4161 TGGTAACATTTCTTTCTGTGCTTGGCTATCTTTGTGGACTTCAGGGGCTTCTAAAACAGACAGGACTGTGTTGCCTTTAC
4241 TAAATGGTCTGAGACAGCTATGGTTTTGAATTTTTAGTTTTTTTTTTTTAACCCACTTCCCCTCCTGGTCTCTTCCCTCT
4321 CTGATAATTACCATTCATATGTGAGTGTTAGTGTGCCTCCTTTTAGCATTTTCTTCTTCTCTTTCTGATTCTTCATTTCT
4401 GACTGCCTAGGCAAGGAAACCAGATAACCAAACTTACTAGAACGTTCTTTAAAACACAAGTACAAACTCTGGGACAGGAC
4481 CCAAGACACTTTCCTGTGAAGTGCTGAAAAAGACCTCATTGTATTGGCATTTGATATCAGTTTGATGTAGCTTAGAGTGC
4561 TTCCTGATTCTTGCTGAGTTTCAGGTAGTTGAGATAGAGAGAAGTGAGTCATATTCATATTTTCCCCCTTAGAATAATAT
4641 TTTGAAAGGTTTCATTGCTTCCACTTGAATGCTGCTCTTACAAAAACTGGGGTTACAAGGGTTACTAAATTAGCATCAGT
4721 AGCCAGAGGCAATACCGTTGTCTGGAGGACACCAGCAAACAACACACAACAAAGCAAAACAAACCTTGGGAAACTAAGGC
4801 CATTTGTTTTGTTTTGGTGTCCCCTTTGAAGCCCTGCCTTCTGGCCTTACTCCTGTACAGATATTTTTGACCTATAGGTG
4881 CCTTTATGAGAATTGAGGGTCTGACATCCTGCCCCAAGGAGTAGCTAAAGTAATTGCTAGTGTTTTCAGGGATTTTAACA
4961 TCAGACTGGAATGAATGAATGAAACTTTTTGTCCTTTTTTTTTCTGTTTTTTTTTTTCTAATGTAGTAAGGACTAAGGAA
5041 AACCTTTGGTGAAGACAATCATTTCTCTCTGTTGATGTGGATACTTTTCACACCGTTTATTTAAATGCTTTCTCAATAGG
5121 TCCAGAGCCAGTGTTCTTGTTCAACCTGAAAGTAATGGCTCTGGGTTGGGCCAGACAGTTGCACTCTCTAGTTTGCCCTC
5201 TGCCACAAATTTGATGTGTGACCTTTGGGCAAGTCATTTATCTTCTCTGGGCCTTAGTTGCCTCATCTGTAAAATGAGGG
5281 AGTTGGAGTAGATTAATTATTCCAGCTCTGAAATTCTAAGTGACCTTGGCTACCTTGCAGCAGTTTTGGATTTCTTCCTT
5361 ATCTTTGTTCTGCTGTTTGAGGGGGCTTTTTACTTATTTCCATGTTATTCAAAGGAGACTAGGCTTGATATTTTATTACT
5441 GTTCTTTTATGGACAAAAGGTTACATAGTATGCCCTTAAGACTTAATTTTAACCAAAGGCCTAGCACCACCTTAGGGGCT
5521 GCAATAAACACTTAACGCGCGTGCGCACGCGCGCGCGCACACACACACACACACACACACACACACACAGGTCAGAGTTT
5601 AAGGCTTTCGAGTCATGACATTCTAGCTTTTGAATTGCGTGCACACACACACGCACGCACACACTCTGGTCAGAGTTTAT
5681 TAAGGCTTTCGAGTCATGACATTATAGCTTTTGAGTTGGTGTGTGTGACACCACCCTCCTAAGTGGTGTGTGCTTGTAAT
5761 TTTTTTTTTCAGTGAAAATGGATTGAAAACCTGTTGTTAATGCTTAGTGATATTATGCTCAAAACAAGGAAATTCCCTTG
5841 AACCGTGTCAATTAAACTGGTTTATATGACTCAAGAAAACAATACCAGTAGATGATTATTAACTTTATTCTTGGCTCTTT
5921 TTAGGTCCATTTTGATTAAGTGACTTTTGGCTGGATCATTCAGAGCTCTCTTCTAGCCTACCCTTGGATGAGTACAATTA
6001 ATGAAATTCATATTTTCAAGGACCTGGGAGCCTTCCTTGGGGCTGGGTTGAGGGTGGGGGGTTGGGGAGTCCTGGTAGAG
6081 GCCAGCTTTGTGGTAGCTGGAGAGGAAGGGATGAAACCAGCTGCTGTTGCAAAGGCTGCTTGTCATTGATAGAAGGACTC
6161 ACGGGCTTGGATTGATTAAGACTAAACATGGAGTTGGCAAACTTTCTTCAAGTATTGAGTTCTGTTCAATGCATTGGACA
6241 TGTGATTTAAGGGAAAAGTGTGAATGCTTATAGATGATGAAAACCTGGTGGGCTGCAGAGCCCAGTTTAGAAGAAGTGAG
6321 TTGGGGGTTGGGGACAGATTTGGTGGTGGTATTTCCCAACTGTTTCCTCCCCTAAATTCAGAGGAATGCAGCTATGCCAG
6401 AAGCCAGAGAAGAGCCACTCGTAGCTTCTGCTTTGGGGACAACTGGTCAGTTGAAAGTCCCAGGAGTTCCTTTGTGGCTT
6481 TCTGTATACTTTTGCCTGGTTAAAGTCTGTGGCTAAAAAATAGTCGAACCTTTCTTGAGAACTCTGTAACAAAGTATGTT
6561 TTTGATTAAAAGAGAAAGCCAACTAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-483-3p :: SMAD4    [ Functional MTI ]
Validation Method Luciferase reporter assay , qRT-PCR , Western blot ,
Conditions SW1990 , PANC-1
Disease pancreatic cancer;
Location of target site 3'UTR
Tools used in this research miRanda , PicTar , TargetScan
Original Description (Extracted from the article) ... DPC4/Smad4 is adirect target of miRNA-483-3p// DPC4/Smad4 is identified as a target of miR-483-3p and their expression levels are inversely correlated in human clinical specimens ...

- Hao, J. Zhang, S. Zhou, Y. Hu, X. Shao, C., 2011, FEBS letters.

Article - Hao, J. Zhang, S. Zhou, Y. Hu, X. Shao, C.
- FEBS letters, 2011
Both deregulation of tumor-suppressor genes and misexpression of microRNAs (miRNAs) have been implicated in the development of pancreatic cancer, but their relationship during this process remains less clear. Here, we report that the expression of miR-483-3p is strongly enhanced in pancreatic cancer tissues compared to side normal tissues using a miRNA-array differential analysis. Furthermore, DPC4/Smad4 is identified as a target of miR-483-3p and their expression levels are inversely correlated in human clinical specimens. Ectopic expression of miR-483-3p significantly represses DPC4/Smad4 protein levels in pancreatic cancer cell lines, and simultaneously promotes cell proliferation and colony formation in vitro. Our findings identify miR-483-3p as a potent regulator of DPC4/Smad4, which may provide a novel therapeutic strategy for the treatment of DPC4/Smad4-driven pancreatic cancer.
LinkOut: [PMID: 21112326]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
7 hsa-miR-483-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004118 SMAD4 SMAD family member 4 4 1
MIRT004500 BBC3 BCL2 binding component 3 4 1
MIRT005378 Gmnn geminin 3 1
MIRT005389 Nhp2 NHP2 ribonucleoprotein homolog (yeast) 3 1
MIRT005393 Ube2c ubiquitin-conjugating enzyme E2C 3 1
MIRT006686 PARD3 par-3 partitioning defective 3 homolog (C. elegans) 2 1
MIRT042392 NUDC nuclear distribution gene C homolog (A. nidulans) 1 1