Accession ID: MIRT004242 [miRNA, hsa-miR-143-3p :: COL1A1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-143LinkOut: [miRBase ]
Description Homo sapiens miR-143 stem-loop
Comment This miRNA sequence was predicted based on homology to a verified miRNA from mouse has a 1 nt 3' extension (A), which is incompatible with the genome sequence.
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-143-3p
Mature Sequence 61| UGAGAUGAAGCACUGUAGCUC |81
Evidence Experimental
Experiments Cloned
Putative hsa-miR-143-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol COL1A1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms OI4
Description collagen, type I, alpha 1
Transcript NM_000088    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on COL1A1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of COL1A1
(miRNA target sites are highlighted)
>COL1A1|NM_000088|3'UTR
   1 ACTCCCTCCATCCCAACCTGGCTCCCTCCCACCCAACCAACTTTCCCCCCAACCCGGAAACAGACAAGCAACCCAAACTG
  81 AACCCCCTCAAAAGCCAAAAAATGGGAGACAATTTCACATGGACTTTGGAAAATATTTTTTTCCTTTGCATTCATCTCTC
 161 AAACTTAGTTTTTATCTTTGACCAACCGAACATGACCAAAAACCAAAAGTGCATTCAACCTTACCAAAAAAAAAAAAAAA
 241 AAAAGAATAAATAAATAACTTTTTAAAAAAGGAAGCTTGGTCCACTTGCTTGAAGACCCATGCGGGGGTAAGTCCCTTTC
 321 TGCCCGTTGGGCTTATGAAACCCCAATGCTGCCCTTTCTGCTCCTTTCTCCACACCCCCCTTGGGGCCTCCCCTCCACTC
 401 CTTCCCAAATCTGTCTCCCCAGAAGACACAGGAAACAATGTATTGTCTGCCCAGCAATCAAAGGCAATGCTCAAACACCC
 481 AAGTGGCCCCCACCCTCAGCCCGCTCCTGCCCGCCCAGCACCCCCAGGCCCTGGGGGACCTGGGGTTCTCAGACTGCCAA
 561 AGAAGCCTTGCCATCTGGCGCTCCCATGGCTCTTGCAACATCTCCCCTTCGTTTTTGAGGGGGTCATGCCGGGGGAGCCA
 641 CCAGCCCCTCACTGGGTTCGGAGGAGAGTCAGGAAGGGCCACGACAAAGCAGAAACATCGGATTTGGGGAACGCGTGTCA
 721 ATCCCTTGTGCCGCAGGGCTGGGCGGGAGAGACTGTTCTGTTCCTTGTGTAACTGTGTTGCTGAAAGACTACCTCGTTCT
 801 TGTCTTGATGTGTCACCGGGGCAACTGCCTGGGGGCGGGGATGGGGGCAGGGTGGAAGCGGCTCCCCATTTTATACCAAA
 881 GGTGCTACATCTATGTGATGGGTGGGGTGGGGAGGGAATCACTGGTGCTATAGAAATTGAGATGCCCCCCCAGGCCAGCA
 961 AATGTTCCTTTTTGTTCAAAGTCTATTTTTATTCCTTGATATTTTTCTTTTTTTTTTTTTTTTTTTGTGGATGGGGACTT
1041 GTGAATTTTTCTAAAGGTGCTATTTAACATGGGAGGAGAGCGTGTGCGGCTCCAGCCCAGCCCGCTGCTCACTTTCCACC
1121 CTCTCTCCACCTGCCTCTGGCTTCTCAGGCCTCTGCTCTCCGACCTCTCTCCTCTGAAACCCTCCTCCACAGCTGCAGCC
1201 CATCCTCCCGGCTCCCTCCTAGTCTGTCCTGCGTCCTCTGTCCCCGGGTTTCAGAGACAACTTCCCAAAGCACAAAGCAG
1281 TTTTTCCCCCTAGGGGTGGGAGGAAGCAAAAGACTCTGTACCTATTTTGTATGTGTATAATAATTTGAGATGTTTTTAAT
1361 TATTTTGATTGCTGGAATAAAGCATGTGGAAATGACCCAAACATAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' cucGAUGUCACG-AAGUAGAGu 5'
             :| |  ||| |||||||| 
Target 5' tttTTCCTTTGCATTCATCTCt 3'
138 - 159 154.00 -11.30
2
miRNA  3' cuCGAUGUCACGAAGUAGAGu 5'
            ||| :  |||  |||||| 
Target 5' tgGCTCT--TGCAACATCTCc 3'
587 - 605 123.00 -11.10
3
miRNA  3' cucGAUGUCACGAAGUAGAgu 5'
             | | :||||| |||||  
Target 5' tacCAAAGGTGCTACATCTat 3'
874 - 894 118.00 -12.50
Experimental Support 1 for Non-Functional miRNA-Target Interaction
miRNA:Target hsa-miR-143-3p :: COL1A1    [ Non-Functional MTI ]
Validation Method Immunoblot , Luciferase reporter assay , qRT-PCR
Conditions LX-2
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... As a result, the miR- 29b and -218 precursors inhibited luciferase activity derived from the vectors carrying Col1A1 or SP1 30UTRs (Fig. 3D). In contrast, the miR-143 precursors had no effect on luciferase activity of the vec- tor carrying Col1A1 30UTR (Fig. 3D). ...

- Ogawa, T. Iizuka, M. Sekiya, Y. Yoshizato, et al., 2010, Biochem Biophys Res Commun.

miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1
miRNA  3' cucGAUGUCACG-AAGUAGAGu 5'
             :| |  ||| |||||||| 
Target 5' uuuUUCCUUUGCAUUCAUCUCu 3'
2 - 23
Article - Ogawa, T. Iizuka, M. Sekiya, Y. Yoshizato, et al.
- Biochem Biophys Res Commun, 2010
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression through imperfect base pairing with the 3' untranslated region (3'UTR) of target mRNA. We studied the regulation of alpha 1 (I) collagen (Col1A1) expression by miRNAs in human stellate cells, which are involved in liver fibrogenesis. Among miR-29b, -143, and -218, whose expressions were altered in response to transforming growth factor-beta1 or interferon-alpha stimulation, miR-29b was the most effective suppressor of type I collagen at the mRNA and protein level via its direct binding to Col1A1 3'UTR. miR-29b also had an effect on SP1 expression. These results suggested that miR-29b is involved in the regulation of type I collagen expression by interferon-alpha in hepatic stellate cells. It is anticipated that miR-29b will be used for the regulation of stellate cell activation and lead to antifibrotic therapy.
LinkOut: [PMID: 19913496]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-143-3p :: COL1A1    [ Functional MTI ]
Validation Method Other
Article - Osaki, M. Takeshita, F. Sugimoto, Y. et al.
- Molecular therapy : the journal of the American Society of Gene Therapy, 2011
Pulmonary metastases are the main cause of death in patients with osteosarcoma, however, the molecular mechanisms of metastasis are not well understood. To detect lung metastasis-related microRNA (miRNA) in human osteosarcoma, we compared parental (HOS) and its subclone (143B) human osteosarcoma cell lines showing lung metastasis in a mouse model. miR-143 was the most downregulated miRNA (P < 0.01), and transfection of miR-143 into 143B significantly decreased its invasiveness, but not cell proliferation. Noninvasive optical imaging technologies revealed that intravenous injection of miR-143, but not negative control miRNA, significantly suppressed lung metastasis of 143B (P < 0.01). To search for miR-143 target mRNA in 143B, microarray analyses were performed using an independent RNA pool extracted by two different comprehensive miR-143-target mRNA collecting systems. Western blot analyses revealed that MMP-13 was mostly protein downregulated by miR-143. Immunohistochemistry using clinical samples clearly revealed MMP-13-positive cells in lung metastasis-positive cases, but not in at least three cases showing higher miR-143 expression in the no metastasis group. Taken together, these data indicated that the downregulation of miR-143 correlates with the lung metastasis of human osteosarcoma cells by promoting cellular invasion, probably via MMP-13 upregulation, suggesting that miRNA could be used to develop new molecular targets for osteosarcoma metastasis.
LinkOut: [PMID: 21427707]
Experimental Support 3 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-143-3p :: COL1A1    [ Functional MTI ]
Validation Method
Article - Bakhshandeh, B. Soleimani, M. Paylakhi, S. et al.
- J Genet, 2012
Generating appropriate cartilage for clinical applications to heal skeletal tissue loss is a major health concern. In this regard, cell-based approaches offer a potential therapeutic strategy for cartilage repair, although little is known about the precise mechanism of chondrogenesis. Unrestricted somatic stem cell (USSC) is considered as a suitable candidate because of its potential for differentiating into multiple cell types. Recent studies show that microRNAs (miRNAs) are involved in several biological processes including development and differentiation. To identify the chondro-specific miRNA signature, miRNA patterns of USSCs and differentiated chondrocytes were investigated using microarrays and validation by qPCR. Prior to these analyses, chondrogenic commitment of differentiated USSCs was verified by immunocytochemistry, specific staining and evaluation of some main chondrogenic marker genes. Various in silico explorations (for both putative targets and signalling pathways) and empirical analyses (miRNA transfections followed by qPCR of some chondrogenic indicators) were carried out to support our results. Transient modulation of multiple chondro-miRs (such as mir-630, mir-624 and mir-376) with chondrocyte targets (such as TGFbR, MAP3K, collagens, SMADs and cadherins) as mediators of chondrogenic signalling pathways including cell-cell interactions, TGF-beta, and MAPK signalling suggests a mechanism for genetic induction of chondrogenic differentiation. In conclusion, this research reveals more details about the allocation of USSCs into the chondrocytes through identification of miRNA signature which modulates targets and pathways required for chondrogenic lineage and could provide guidelines for future clinical treatments and anti-miRNA therapies.
LinkOut: [PMID: 22942087]
MiRNA-Target Expression Profile:

 
MiRNA-Target Expression Profile(TCGA):

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
158 hsa-miR-143-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000312 KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog 5 4
MIRT000314 KLF4 Kruppel-like factor 4 (gut) 1 2
MIRT000458 MYO6 myosin VI 4 7
MIRT000716 DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha 4 3
MIRT003060 FNDC3B fibronectin type III domain containing 3B 4 3
MIRT003759 MAPK7 mitogen-activated protein kinase 7 5 16
MIRT003964 COX2 cytochrome c oxidase subunit II 1 1
MIRT004242 COL1A1 collagen, type I, alpha 1 4 3
MIRT005644 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog 3 2
MIRT005771 FSCN1 fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) 3 2
MIRT006301 HK2 hexokinase 2 3 5
MIRT006316 SERPINE1 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 3 1
MIRT006362 FHIT fragile histidine triad 1 1
MIRT006469 MACC1 metastasis associated in colon cancer 1 3 1
MIRT006757 PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) 3 2
MIRT006846 JAG1 jagged 1 4 1
MIRT007179 AKT1 v-akt murine thymoma viral oncogene homolog 1 3 1
MIRT007256 MDM2 Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) 1 1
MIRT007348 BCL2 B-cell CLL/lymphoma 2 4 4
MIRT021540 MMP13 matrix metallopeptidase 13 (collagenase 3) 3 2
MIRT052912 SDC1 syndecan 1 3 1
MIRT053082 RREB1 ras responsive element binding protein 1 3 1
MIRT053215 CD44 CD44 molecule (Indian blood group) 3 1
MIRT053217 KLF5 Kruppel-like factor 5 (intestinal) 3 1
MIRT053218 BRAF v-raf murine sarcoma viral oncogene homolog B1 3 1
MIRT053639 MAP3K7 mitogen-activated protein kinase kinase kinase 7 1 1
MIRT053640 CHST10 carbohydrate sulfotransferase 10 1 1
MIRT053641 MAPK1 mitogen-activated protein kinase 1 2 3
MIRT053642 COL5A1 collagen, type V, alpha 1 1 1
MIRT053643 PDGFRA platelet-derived growth factor receptor, alpha polypeptide 1 1
MIRT053644 SMAD3 SMAD family member 3 1 1
MIRT053645 CTNND1 catenin (cadherin-associated protein), delta 1 1 1
MIRT053646 COL5A2 collagen, type V, alpha 2 1 1
MIRT053647 ERBB3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) 1 1
MIRT054257 IGF1R insulin-like growth factor 1 receptor 3 1
MIRT083963 RAB22A RAB22A, member RAS oncogene family 1 2
MIRT095674 RBM27 RNA binding motif protein 27 1 2
MIRT104018 USP42 ubiquitin specific peptidase 42 1 3
MIRT114769 CMPK1 cytidine monophosphate (UMP-CMP) kinase 1, cytosolic 1 1
MIRT178097 SAMD8 sterile alpha motif domain containing 8 1 1
MIRT192240 BTF3L4 basic transcription factor 3-like 4 1 2
MIRT197416 KAT7 K(lysine) acetyltransferase 7 1 1
MIRT294390 ZNF264 zinc finger protein 264 1 2
MIRT348502 CALM3 calmodulin 3 (phosphorylase kinase, delta) 1 1
MIRT364788 PRRC2B proline-rich coiled-coil 2B 1 1
MIRT392564 ORAI2 ORAI calcium release-activated calcium modulator 2 1 1
MIRT437418 TNF tumor necrosis factor 2 1
MIRT437449 NR2C2 nuclear receptor subfamily 2, group C, member 2 3 1
MIRT438056 IL13RA1 interleukin 13 receptor, alpha 1 2 1
MIRT438300 DYT10 dystonia 10 1 1
MIRT438362 COL3A1 collagen, type III, alpha 1 2 1
MIRT438363 DDX6 DEAD (Asp-Glu-Ala-Asp) box helicase 6 3 1
MIRT438605 LIMK1 LIM domain kinase 1 2 1
MIRT438817 HNF4A hepatocyte nuclear factor 4, alpha 1 1
MIRT443068 CASP5 caspase 5, apoptosis-related cysteine peptidase 1 1
MIRT443952 LRIT3 leucine-rich repeat, immunoglobulin-like and transmembrane domains 3 1 1
MIRT444773 NAPEPLD N-acyl phosphatidylethanolamine phospholipase D 1 1
MIRT446698 PAPPA pregnancy-associated plasma protein A, pappalysin 1 1 1
MIRT447386 VOPP1 vesicular, overexpressed in cancer, prosurvival protein 1 1 1
MIRT449324 FAM120AOS family with sequence similarity 120A opposite strand 1 1
MIRT449720 C1orf61 chromosome 1 open reading frame 61 1 1
MIRT450534 PGLS 6-phosphogluconolactonase 1 1
MIRT455653 YARS tyrosyl-tRNA synthetase 1 1
MIRT460080 HAAO 3-hydroxyanthranilate 3,4-dioxygenase 1 1
MIRT461002 SYT7 synaptotagmin VII 1 1
MIRT463471 ZC3HAV1L zinc finger CCCH-type, antiviral 1-like 1 1
MIRT464503 UCK2 uridine-cytidine kinase 2 1 1
MIRT466680 TAF1D TATA box binding protein (TBP)-associated factor, RNA polymerase I, D, 41kDa 1 2
MIRT467792 SLC2A14 solute carrier family 2 (facilitated glucose transporter), member 14 1 1
MIRT467864 SLC25A25 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 1 1
MIRT468170 SGPL1 sphingosine-1-phosphate lyase 1 1 1
MIRT468661 SECISBP2L SECIS binding protein 2-like 1 3
MIRT469389 RER1 RER1 retention in endoplasmic reticulum 1 homolog (S. cerevisiae) 1 1
MIRT470349 PPP2R5E protein phosphatase 2, regulatory subunit B', epsilon isoform 1 1
MIRT472272 NFIC nuclear factor I/C (CCAAT-binding transcription factor) 1 1
MIRT472421 NCKAP1 NCK-associated protein 1 1 1
MIRT474387 KLK10 kallikrein-related peptidase 10 1 1
MIRT475966 GXYLT1 glucoside xylosyltransferase 1 1 2
MIRT478706 CSRNP2 cysteine-serine-rich nuclear protein 2 1 1
MIRT481012 BBC3 BCL2 binding component 3 1 2
MIRT483101 TFPI tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) 1 1
MIRT490496 GLRA3 glycine receptor, alpha 3 1 1
MIRT492650 PLEKHM3 pleckstrin homology domain containing, family M, member 3 1 1
MIRT497536 ZNF607 zinc finger protein 607 1 1
MIRT500647 TUBB2A tubulin, beta 2A class IIa 1 3
MIRT512678 ENO4 enolase family member 4 1 1
MIRT516289 DBT dihydrolipoamide branched chain transacylase E2 1 1
MIRT516446 ADAMTS4 ADAM metallopeptidase with thrombospondin type 1 motif, 4 1 2
MIRT516980 OR7D2 olfactory receptor, family 7, subfamily D, member 2 1 1
MIRT519473 SPTLC2 serine palmitoyltransferase, long chain base subunit 2 1 1
MIRT520114 XIAP X-linked inhibitor of apoptosis 1 2
MIRT521965 PHAX phosphorylated adaptor for RNA export 1 2
MIRT528757 RPS27 ribosomal protein S27 1 3
MIRT529372 YWHAB tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide 1 2
MIRT537167 GGA2 golgi-associated, gamma adaptin ear containing, ARF binding protein 2 1 1
MIRT539673 ZBTB44 zinc finger and BTB domain containing 44 1 2
MIRT546394 STOX2 storkhead box 2 1 2
MIRT555989 NFYB nuclear transcription factor Y, beta 1 1
MIRT562149 IGFBP5 insulin-like growth factor binding protein 5 1 1
MIRT567218 IER5 immediate early response 5 1 1
MIRT568005 COMMD2 COMM domain containing 2 1 1
MIRT571032 CENPP centromere protein P 1 1
MIRT572784 ZNF277 zinc finger protein 277 1 1
MIRT573165 SLC30A9 solute carrier family 30 (zinc transporter), member 9 1 1
MIRT609129 NUDT3 nudix (nucleoside diphosphate linked moiety X)-type motif 3 1 1
MIRT609287 OAS3 2'-5'-oligoadenylate synthetase 3, 100kDa 1 1
MIRT613433 GALNT6 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6) 1 1
MIRT613773 TTC38 tetratricopeptide repeat domain 38 1 1
MIRT616648 LRAT lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) 1 2
MIRT620622 ADH5 alcohol dehydrogenase 5 (class III), chi polypeptide 1 1
MIRT630895 SLC25A33 solute carrier family 25 (pyrimidine nucleotide carrier), member 33 1 1
MIRT636529 FAXC failed axon connections homolog (Drosophila) 1 2
MIRT641397 NUBPL nucleotide binding protein-like 1 1
MIRT641415 SCN2B sodium channel, voltage-gated, type II, beta subunit 1 1
MIRT642531 CERS4 ceramide synthase 4 1 1
MIRT643189 HYPK huntingtin interacting protein K 1 1
MIRT652153 TRIM71 tripartite motif containing 71, E3 ubiquitin protein ligase 1 1
MIRT652605 TIMM8A translocase of inner mitochondrial membrane 8 homolog A (yeast) 1 1
MIRT659374 CREG2 cellular repressor of E1A-stimulated genes 2 1 1
MIRT661609 C2orf15 chromosome 2 open reading frame 15 1 1
MIRT666343 SKAP2 src kinase associated phosphoprotein 2 1 1
MIRT670417 ELP2 elongator acetyltransferase complex subunit 2 1 1
MIRT671125 ZNF573 zinc finger protein 573 1 1
MIRT671158 ANKRD9 ankyrin repeat domain 9 1 1
MIRT671341 FAM71F2 family with sequence similarity 71, member F2 1 1
MIRT671872 ZNF429 zinc finger protein 429 1 1
MIRT671977 IKZF3 IKAROS family zinc finger 3 (Aiolos) 1 1
MIRT672067 KIAA0930 KIAA0930 1 1
MIRT672657 SLC25A16 solute carrier family 25 (mitochondrial carrier; Graves disease autoantigen), member 16 1 2
MIRT672676 GTF2H5 general transcription factor IIH, polypeptide 5 1 1
MIRT672774 UBE2V2 ubiquitin-conjugating enzyme E2 variant 2 1 1
MIRT672932 LRRC2 leucine rich repeat containing 2 1 1
MIRT673162 C1orf50 chromosome 1 open reading frame 50 1 1
MIRT673275 RUNDC1 RUN domain containing 1 1 1
MIRT673335 THAP1 THAP domain containing, apoptosis associated protein 1 1 1
MIRT673354 SLC35F6 chromosome 2 open reading frame 18 1 1
MIRT673670 ZNF440 zinc finger protein 440 1 1
MIRT673907 DCTN6 dynactin 6 1 1
MIRT674099 PLEKHA1 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1 1 1
MIRT674404 MYCBP c-myc binding protein 1 1
MIRT674528 PRR23A proline rich 23A 1 1
MIRT674796 NPR1 natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) 1 1
MIRT675069 FGD6 FYVE, RhoGEF and PH domain containing 6 1 1
MIRT675083 CCR6 chemokine (C-C motif) receptor 6 1 1
MIRT675129 FSD2 fibronectin type III and SPRY domain containing 2 1 1
MIRT679404 IL10RB interleukin 10 receptor, beta 1 1
MIRT681189 MYPN myopalladin 1 1
MIRT689232 RPS19 ribosomal protein S19 1 1
MIRT694011 PPIL4 peptidylprolyl isomerase (cyclophilin)-like 4 1 1
MIRT699674 SFT2D2 SFT2 domain containing 2 1 1
MIRT700377 RAB33B RAB33B, member RAS oncogene family 1 1
MIRT701996 MIER3 mesoderm induction early response 1, family member 3 1 1
MIRT706216 ACOT9 acyl-CoA thioesterase 9 1 1
MIRT706551 GJD2 gap junction protein, delta 2, 36kDa 1 1
MIRT707421 RRP7A ribosomal RNA processing 7 homolog A (S. cerevisiae) 1 1
MIRT710651 GLUL glutamate-ammonia ligase 1 1
MIRT720169 PNPO pyridoxamine 5'-phosphate oxidase 1 1
MIRT724395 ABAT 4-aminobutyrate aminotransferase 1 1
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