Accession ID: MIRT004318 [miRNA, hsa-miR-21 :: ERBB2, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-21 LinkOut: [miRBase ]
Synonyms MIRN21, hsa-mir-21, miR-21, miRNA21, MIR21
Description Homo sapiens miR-21 stem-loop
Comment Mourelatos et al. named this sequence miR-21 precursor-17 and also reported the exact reverse complement of this predicted stem-loop sequence and erroneously assigned the name miR-104 .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-21-3p
Mature Sequence 46| CAACACCAGUCGAUGGGCUGU |66
Evidence Experimental
Experiments Cloned
Putative hsa-miR-21-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-21-5p
Mature Sequence 8| UAGCUUAUCAGACUGAUGUUGA |29
Evidence Experimental
Experiments Cloned
Putative hsa-miR-21-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol ERBB2 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1
Description v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Transcript NM_001005862   LinkOut: [ RefSeq ]
Other Transcripts NM_004448   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04012    ErbB signaling pathway - Homo sapiens (human)
hsa04020    Calcium signaling pathway - Homo sapiens (human)
hsa04510    Focal adhesion - Homo sapiens (human)
hsa04520    Adherens junction - Homo sapiens (human)
hsa05200    Pathways in cancer - Homo sapiens (human)
hsa05212    Pancreatic cancer - Homo sapiens (human)
hsa05213    Endometrial cancer - Homo sapiens (human)
hsa05215    Prostate cancer - Homo sapiens (human)
hsa05219    Bladder cancer - Homo sapiens (human)
hsa05223    Non-small cell lung cancer - Homo sapiens (human)
Putative miRNA Targets on ERBB2 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of ERBB2
(miRNA target sites are highlighted)		 
>ERBB2|NM_001005862|3'UTR
   1 TGAACCAGAAGGCCAAGTCCGCAGAAGCCCTGATGTGTCCTCAGGGAGCAGGGAAGGCCTGACTTCTGCTGGCATCAAGA
  81 GGTGGGAGGGCCCTCCGACCACTTCCAGGGGAACCTGCCATGCCAGGAACCTGTCCTAAGGAACCTTCCTTCCTGCTTGA
 161 GTTCCCAGATGGCTGGAAGGGGTCCAGCCTCGTTGGAAGAGGAACAGCACTGGGGAGTCTTTGTGGATTCTGAGGCCCTG
 241 CCCAATGAGACTCTAGGGTCCAGTGGATGCCACAGCCCAGCTTGGCCCTTTCCTTCCAGATCCTGGGTACTGAAAGCCTT
 321 AGGGAAGCTGGCCTGAGAGGGGAAGCGGCCCTAAGGGAGTGTCTAAGAACAAAAGCGACCCATTCAGAGACTGTCCCTGA
 401 AACCTAGTACTGCCCCCCATGAGGAAGGAACAGCAATGGTGTCAGTATCCAGGCTTTGTACAGAGTGCTTTTCTGTTTAG
 481 TTTTTACTTTTTTTGTTTTGTTTTTTTAAAGATGAAATAAAGACCCAGGGGGAGAATGGGTGTTGTATGGGGAGGCAAGT
 561 GTGGGGGGTCCTTCTCCACACCCACTTTGTCCATTTGCAAATATATTTTGGAAAACAGCTA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' ugucggGUAGCUGACCACAac 5'
                || | | ||||||  
Target 5' aaggaaCAGC-AATGGTGTca 3'
 		425 - 444 126.00 -11.20
2 
miRNA  3' ugucggguagcugaCCACAAc 5'
                        |||||| 
Target 5' ccagggggagaatgGGTGTTg 3'
 		525 - 545 120.00 -13.20
3 
miRNA  3' ugUCGGGUAGCU----GACCACAac 5'
            ||:||:  ||    ||| |||  
Target 5' caAGTCCGCAGAAGCCCTGATGTgt 3'
 		14 - 38 105.00 -11.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-21 :: ERBB2    [ Functional MTI ]
Validation Method qRT-PCR , Western blot
Conditions MDA-MIB-435
Original Description (Extracted from the article) ... We identified a putative oncogenic miRNA, miR-21, whose expression is correlated with HER2/neu up-regulation and is functionally involved in HER2/neu-induced cell invasion. We show that miR-21 is up-regulated via the MAPK (ERK1/2) pathway upon stimulation of HER2/neu signaling in breast cancer cells ...

- Huang, T. H. Wu, F. Loeb, G. B. Hsu, R. et al., 2009, J Biol Chem.
Article - Huang, T. H. Wu, F. Loeb, G. B. Hsu, R. et al.
- J Biol Chem, 2009
The cell surface receptor tyrosine kinase HER2/neu enhances tumor metastasis. Recent studies suggest that deregulated microRNA (miRNA) expression promotes invasion and metastasis of cancer cells; we therefore explored the possibility that HER2/neu signaling induces the expression of specific miRNAs involved in this process. We identified a putative oncogenic miRNA, miR-21, whose expression is correlated with HER2/neu up-regulation and is functionally involved in HER2/neu-induced cell invasion. We show that miR-21 is up-regulated via the MAPK (ERK1/2) pathway upon stimulation of HER2/neu signaling in breast cancer cells, and overexpression of other ERK1/2 activators such as RASV12 or ID-1 is sufficient to induce miR-21 up-regulation in HER2/neu-negative breast cancer cells. Furthermore, the metastasis suppressor protein PDCD4 (programmed cell death 4) is down-regulated by miR-21 in breast cancer cells expressing HER2/neu. Our data reveal a mechanism for HER2/neu-induced cancer cell invasion via miRNA deregulation. In addition, our results identify miR-21 as a potential therapeutic target for the prevention of breast cancer invasion and metastasis.
LinkOut: [PMID: 19419954]