Accession ID: MIRT004363 [miRNA, hsa-miR-125b ::
AKT1, target gene]
| miRNA name | hsa-miR-125b |
|---|
 |
| Gene Symbol | AKT1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] |
|---|---|
| Synonyms | AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA |
| Description | v-akt murine thymoma viral oncogene homolog 1 |
| Transcript | NM_005163 LinkOut: [ RefSeq ] |
| Other Transcripts | NM_001014432, NM_001014431 |
| Expression | LinkOut: [ BioGPS ] |
| KEGG Pathway |
hsa04010 MAPK signaling pathway - Homo sapiens (human) hsa04012 ErbB signaling pathway - Homo sapiens (human) hsa04062 Chemokine signaling pathway - Homo sapiens (human) hsa04150 mTOR signaling pathway - Homo sapiens (human) hsa04210 Apoptosis - Homo sapiens (human) hsa04370 VEGF signaling pathway - Homo sapiens (human) hsa04510 Focal adhesion - Homo sapiens (human) hsa04530 Tight junction - Homo sapiens (human) hsa04620 Toll-like receptor signaling pathway - Homo sapiens (human) hsa04630 Jak-STAT signaling pathway - Homo sapiens (human) hsa04660 T cell receptor signaling pathway - Homo sapiens (human) hsa04662 B cell receptor signaling pathway - Homo sapiens (human) hsa04664 Fc epsilon RI signaling pathway - Homo sapiens (human) hsa04666 Fc gamma R-mediated phagocytosis - Homo sapiens (human) hsa04722 Neurotrophin signaling pathway - Homo sapiens (human) hsa04910 Insulin signaling pathway - Homo sapiens (human) hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human) hsa04920 Adipocytokine signaling pathway - Homo sapiens (human) hsa05142 Chagas disease - Homo sapiens (human) hsa05200 Pathways in cancer - Homo sapiens (human) hsa05210 Colorectal cancer - Homo sapiens (human) hsa05211 Renal cell carcinoma - Homo sapiens (human) hsa05212 Pancreatic cancer - Homo sapiens (human) hsa05213 Endometrial cancer - Homo sapiens (human) hsa05214 Glioma - Homo sapiens (human) hsa05215 Prostate cancer - Homo sapiens (human) hsa05218 Melanoma - Homo sapiens (human) hsa05220 Chronic myeloid leukemia - Homo sapiens (human) hsa05221 Acute myeloid leukemia - Homo sapiens (human) hsa05222 Small cell lung cancer - Homo sapiens (human) hsa05223 Non-small cell lung cancer - Homo sapiens (human) |
| Putative miRNA Targets on AKT1 | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] |
| 3'UTR of AKT1 (miRNA target sites are highlighted) |
>AKT1|NM_005163|3'UTR 1 TCGGCCAGCGGCACGGCCTGAGGCGGCGGTGGACTGCGCTGGACGATAGCTTGGAGGGATGGAGAGGCGGCCTCGTGCCA 81 TGATCTGTATTTAATGGTTTTTATTTCTCGGGTGCATTTGAGAGAAGCCACGCTGTCCTCTCGAGCCCAGATGGAAAGAC 161 GTTTTTGTGCTGTGGGCAGCACCCTCCCCCGCAGCGGGGTAGGGAAGAAAACTATCCTGCGGGTTTTAATTTATTTCATC 241 CAGTTTGTTCTCCGGGTGTGGCCTCAGCCCTCAGAACAATCCGATTCACGTAGGGAAATGTTAAGGACTTCTGCAGCTAT 321 GCGCAATGTGGCATTGGGGGGCCGGGCAGGTCCTGCCCATGTGTCCCCTCACTCTGTCAGCCAGCCGCCCTGGGCTGTCT 401 GTCACCAGCTATCTGTCATCTCTCTGGGGCCCTGGGCCTCAGTTCAACCTGGTGGCACCAGATGCAACCTCACTATGGTA 481 TGCTGGCCAGCACCCTCTCCTGGGGGTGGCAGGCACACAGCAGCCCCCCAGCACTAAGGCCGTGTCTCTGAGGACGTCAT 561 CGGAGGCTGGGCCCCTGGGATGGGACCAGGGATGGGGGATGGGCCAGGGTTTACCCAGTGGGACAGAGGAGCAAGGTTTA 641 AATTTGTTATTGTGTATTATGTTGTTCAAATGCATTTTGGGGGTTTTTAATCTTTGTGACAGGAAAGCCCTCCCCCTTCC 721 CCTTCTGTGTCACAGTTCTTGGTGACTGTCCCACCGGGAGCCTCCCCCTCAGATGATCTCTCCACGGTAGCACTTGACCT 801 TTTCGACGCTTAACCTTTCCGCTGTCGCCCCAGGCCCTCCCTGACTCCCTGTGGGGGTGGCCATCCCTGGGCCCCTCCAC 881 GCCTCCTGGCCAGACGCTGCCGCTGCCGCTGCACCACGGCGTTTTTTTACAACATTCAACTTTAGTATTTTTACTATTAT 961 AATATAATATGGAACCTTCCCTCCAAATTCTTCAATAAAAGTTGCTTTTCAAAAAAAAAAAAAAAAAAAAAA Target sites Provided by authors Predicted by miRanda |
| miRNA:Target | hsa-miR-125b :: AKT1 [ Functional MTI ] |
|---|---|
| Validation Method | qRT-PCR , Western blot |
| Conditions | HepG2, SK-Hep-1 |
| Original Description (Extracted from the article) | ... The results showed that mir-125b could obviously suppress the phosporylation of Akt. Summarizing these results, this study implicated that miR-125b might suppress cancer cell proliferation through inactivating Akt. ... - Li, W. Xie, L. He, X. Li, J. Tu, K. Wei, L. et al., 2008, Int J Cancer. |
| Article |
- Li, W.
Xie, L.
He, X.
Li, J.
Tu, K.
Wei, L. et al. - Int J Cancer, 2008
MicroRNAs (miRNAs) are important gene regulators, which are often deregulated in cancers. In this study, the authors analyzed the microRNAs profiles of 78 matched cancer/noncanerous liver tissues from HCC patients and 10 normal liver tissues and found that 69 miRNAs were differentially expressed between hepatocellular carcinoma (HCC) and corresponding noncancerous liver tissues (N). Then the expressions of 8 differentially expressed miRNAs were validated by real time RT PCR. The set of differentially expressed miRNAs could distinctly classify HCC, N and normal liver tissues (NL). Moreover, some of these differentially expressed miRNAs were related to the clinical factors of HCC patients. Most importantly, Kaplan-Meier estimates and the log-rank test showed that high expression of hsa-miR-125b was correlated with good survival of HCC patients (hazard ratio, 1.787, 95% confidence interval, 1.020-3.133, p = 0.043). The transfection assay showed that overexpression of miR-125b in HCC cell line could obviously suppress the cell growth and phosporylation of Akt. In conclusion, the authors have demonstrated the diagnostic miRNA profile for HCC, and for the first time, identified the miR-125b with predictive significance for HCC prognosis.
LinkOut: [PMID: 18649363]
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