Accession ID: MIRT004408 [miRNA, hsa-miR-125b :: CYP24A1, target gene]
|Synonyms||CP24, CYP24, MGC126273, MGC126274, P450-CC24|
|Description||cytochrome P450, family 24, subfamily A, polypeptide 1|
|Putative miRNA Targets on CYP24A1|
|3'UTR of CYP24A1
(miRNA target sites are highlighted)
>CYP24A1|NM_000782|3'UTR 1 TAATACGCCTCAGATGGTGGTATTTGCTAACATCATATCCAACTCAGGGAAGCGGACTGAGTGCTGGGATCCAAGGCATT 81 CTACAGGGTTCACTGCTGGTTTACACTTCACCTGTGTCAGCACCATCTTCAGGTGCTTAGAATGGCCTGGGAGCCTGTTC 161 TGTCTTGCATCTTCCATGACATGAAAGGGAGGCTGGCACTTGTCAGTCAGGTAGAGGTTACAAACCGTTTCAGGCCCTGC 241 CTACCACATTCACTGTTTGAATCTTTAATTCCCAAGAATAAGTTTACATTTCACAATGAATGACCTACAACAGCTAAATT 321 TTCTGGGGCTGGGAGTAATACTGACAATCCATTTACTGTAGCTCTGCTTAATGTACTACTTAGGAAAATGTCCCTGCTTA 401 ATAATGTAAGCCAAGCTAAATGATGGTTAAAGTTATCAGGCCTCCCATGAAATTGCGTTCTTCCTGCATTGAAATAAAAA 481 CATTATTGGGAAACTAGAGAACACCTCTATTTTTAAAAGGACTTTAACGAAGTCAAACAACTTATAAGACTAGTGATTCA 561 CTGGGGCATTATTTTGTTAGAGGACCTTAAAATTGTTTATTTTTTAAATGTGATTCCTTTATGGCATTAGGGTAAAGATG 641 AAGCAATAATTTTTAAATTGTGTATGTGCATATGAAGCACAGACATGCATGTGTGTGTGTGTCTGTGTGTGTGTGTCCGT 721 GTATGTGTGTGTGGGTTCTAATGGTAATTTGCCTCAGTCATTTTTTTAATATTTGCAGTACTTGATTTAGGATCTGTGGT 801 GCAGGGCAATGTTTCAAAGTTTAGTCACAGCTTAAAAACATTCAGTGTGACTTTAATATTATAAAATGATTTCCCATGCC 881 ATAATTTTTCTGTCTATTAAATGGGACAAGTGTAAAGCATGCAAAAGTTAGAGATCTGTTATATAACATTTGTTTTGTGA 961 TTTGAACTCCTAGGAAAAATATGATTTCATAAATGTAAAATGCACAGAAATGCATGCAATACTTATAAGACTTAAAAATT 1041 GTGTTTACAGATGGTTTATTTGTGCATATTTTTACTACTGCTTTTCCTAAATGCATACTGTATATAATTCTGTGTATTTG 1121 ATAAATATTTCTTCCTACATTATATTTTTAGAATATTTCAGAAATATACATTTATGTCTTTATATTGTAATAAATATGTA 1201 CATATCTAGGTATATGCTTTCTCTCTGCTGTGAAATTATTTTTAGAATTATAAATTCACGTCTTGTCAGATTTCATCTGT 1281 ATACCTTCAAATTCTCTGAAAGTAAAAATAAAAGTTTTTAAATATTAAAAAAAAAAAAAAAAAAAAA
|miRNA:Target||hsa-miR-125b :: CYP24A1 [ Functional MTI ]|
|Validation Method||qRT-PCR , Luciferase reporter assay , Western blot , Northern blot|
|Location of target site||3'UTR|
|Tools used in this research||TargetScan|
|Original Description (Extracted from the article)||... These results suggest that miR-125b recognized the MRE125b on the human CYP24 mRNA and regulated the expression ...
- Komagata, S. Nakajima, M. Takagi, S. Mohri, et al., 2009, Mol Pharmacol.
|miRNA-target interactions (Provided by authors)||
Human CYP24 catalyzing the inactivation of calcitriol is post-transcriptionally regulated by miR-125b- Komagata, S. Nakajima, M. Takagi, S. Mohri, et al.
- Mol Pharmacol, 2009
Human vitamin D3 hydroxylase (CYP24) catalyzes the inactivation of 1alpha,25-dihydroxyvitamin D3 (calcitriol), which exerts antiproliferative effects. CYP24 has been reported to be overexpressed in various cancers in which microRNA levels are dysregulated. In silico analysis identified a potential miR-125b recognition element (MRE125b) in the 3'-untranslated region of human CYP24 mRNA. We investigated whether CYP24 is regulated by miR-125b. In luciferase assays using a reporter plasmid containing MRE125b, transfection of the antisense oligonucleotide (AsO) for miR-125b increased the reporter activity in KGN cells, and transfection of precursor miR-125b decreased the reporter activity in MCF-7 cells. The endogenous CYP24 protein level was also increased by AsO for miR-125b in KGN cells and was decreased by precursor miR-125b in MCF-7 cells. These results suggested that human CYP24 is regulated by miR-125b. Immunohistochemical analysis revealed that the CYP24 protein levels in human breast cancer were higher than in adjacent normal tissues, without an accompanying CYP24 mRNA increase. On the other hand, the expression levels of miR-125b in cancer tissues were significantly (P < 0.0005) lower than those in normal tissues. It is noteworthy that the CYP24 protein levels in cancer tissues were inversely associated with the cancer/normal ratios of the miR-125b levels, indicating that the decreased miR-125b levels in breast cancer tissues would be one of the causes of the high CYP24 protein expression. In conclusion, this study clearly demonstrates that miR-125b post-transcriptionally regulates the CYP24, which serves as a possible mechanism for the high CYP24 expression in cancer tissues.LinkOut: [PMID: 19570947]