Accession ID: MIRT004409 [miRNA, hsa-miR-146a :: IL8, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-146a LinkOut: [miRBase ]
Synonyms MIRN146, MIRN146A, miR-146a, miRNA146A, MIR146A
Description Homo sapiens miR-146a stem-loop
Comment This miRNA sequence is predicted based on homology to a verified miRNA from mouse .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-146a-3p
Mature Sequence 57| CCUCUGAAAUUCAGUUCUUCAG |78
Evidence Experimental
Experiments Cloned
Putative hsa-miR-146a-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-146a-5p
Mature Sequence 21| UGAGAACUGAAUUCCAUGGGUU |42
Evidence Experimental
Experiments Cloned
Putative hsa-miR-146a-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol IL8 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1
Description interleukin 8
Transcript NM_000584   LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04060    Cytokine-cytokine receptor interaction - Homo sapiens (human)
hsa04062    Chemokine signaling pathway - Homo sapiens (human)
hsa04620    Toll-like receptor signaling pathway - Homo sapiens (human)
hsa04621    NOD-like receptor signaling pathway - Homo sapiens (human)
hsa04622    RIG-I-like receptor signaling pathway - Homo sapiens (human)
hsa05120    Epithelial cell signaling in Helicobacter pylori infection - Homo sapiens (human)
hsa05142    Chagas disease - Homo sapiens (human)
hsa05200    Pathways in cancer - Homo sapiens (human)
hsa05219    Bladder cancer - Homo sapiens (human)
Putative miRNA Targets on IL8 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of IL8
(miRNA target sites are highlighted)		 
>IL8|NM_000584|3'UTR
   1 TAAAAAAATTCATTCTCTGTGGTATCCAAGAATCAGTGAAGATGCCAGTGAAACTTCAAGCAAATCTACTTCAACACTTC
  81 ATGTATTGTGTGGGTCTGTTGTAGGGTTGCCAGATGCAATACAAGATTCCTGGTTAAATTTGAATTTCAGTAAACAATGA
 161 ATAGTTTTTCATTGTACCATGAAATATCCAGAACATACTTATATGTAAAGTATTATTTATTTGAATCTACAAAAAACAAC
 241 AAATAATTTTTAAATATAAGGATTTTCCTAGATATTGCACGGGAGAATATACAAATAGCAAAATTGAGGCCAAGGGCCAA
 321 GAGAATATCCGAACTTTAATTTCAGGAATTGAATGGGTTTGCTAGAATGTGATATTTGAAGCATCACATAAAAATGATGG
 401 GACAATAAATTTTGCCATAAAGTCAAATTTAGCTGGAAATCCTGGATTTTTTTCTGTTAAATCTGGCAACCCTAGTCTGC
 481 TAGCCAGGATCCACAAGTCCTTGTTCCACTGTGCCTTGGTTTCTCCTTTATTTCTAAGTGGAAAAAGTATTAGCCACCAT
 561 CTTACCTCACAGTGATGTTGTGAGGACATGTGGAAGCACTTTAAGTTTTTTCATCATAACATAAATTATTTTCAAGTGTA
 641 ACTTATTAACCTATTTATTATTTATGTATTTATTTAAGCATCAAATATTTGTGCAAGAATTTGGAAAAATAGAAGATGAA
 721 TCATTGATTGAATAGTTATAAAGATGTTATAGTAAATTTATTTTATTTTAGATATTAAATGATGTTTTATTAGATAAATT
 801 TCAATCAGGGTTTTTAGATTAAACAAACAAACAATTGGGTACCCAGTTAAATTTTCATTTCAGATAAACAACAAATAATT
 881 TTTTAGTATAAGTACATTATTGTTTATCTGAAATTTTAATTGAACTAACAATCCTAGTTTGATACTCCCAGTCTTGTCAT
 961 TGCCAGCTGTGTTGGTAGTGCTGTGTTGAATTACGGAATAATGAGTTAGAACTATTAAAACAGCCAAAACTCCACAGTCA
1041 ATATTAGTAATTTCTTGCTGGTTGAAACTTGTTTATTATGTACAAATAGATTCTTATAATATTATTTAAATGACTGCATT
1121 TTTAAATACAAGGCTTTATATTTTTAACTTTAAGATGTTTTTATGTGCTCTCCAAATTTTTTTTACTGTTTCTGATTGTA
1201 TGGAAATATAAAAGTAAATATGAAACATTTAAAATATAATTTGTTGTCAAAGTAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' gacuUCUUGACUUAAAGUCUcc 5'
              | | :|  ||||||||  
Target 5' agttAAATTTTCATTTCAGAta 3'
 		845 - 866 134.00 -5.50
2 
miRNA  3' gacuuCUUG-ACUUAAAGUCUcc 5'
               |||| | ||||||||:  
Target 5' tatccGAACTTTAATTTCAGGaa 3'
 		326 - 348 133.00 -10.70
3 
miRNA  3' gacuUCUUGACUUAAAGUCucc 5'
              | | :||||||||||   
Target 5' ggttAAATTTGAATTTCAGtaa 3'
 		132 - 153 130.00 -11.32
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-146a :: IL8    [ Functional MTI ]
Validation Method Western blot , Northern blot
Conditions MDA-MB-231
Original Description (Extracted from the article) ... These findings implicate miR-146a/b as a negative regulator of constitutive NF-kB activity in a breast cancer setting and suggest that modulating miR-146a/b levels has therapeutic potential to suppress breast cancer metastases.//Although this observed reduction in MDA-MB invasiveness likely derives in large part from miR-146a/b-targeted suppression of the upstream NF-kB signaling proteins IRAK1 and TRAF6 ...

- Bhaumik, D. Scott, G. K. Schokrpur, S. et al., 2008, Oncogene.
Article - Bhaumik, D. Scott, G. K. Schokrpur, S. et al.
- Oncogene, 2008
Cancer cells often acquire a constitutively active nuclear factor-kappaB (NF-kappaB) program to promote survival, proliferation and metastatic potential by mechanisms that remain largely unknown. Extending observations from an immunologic setting, we demonstrate that microRNA-146a and microRNA-146b (miR-146a/b) when expressed in the highly metastatic human breast cancer cell line MDA-MB-231 function to negatively regulate NF-kappaB activity. Lentiviral-mediated expression of miR-146a/b significantly downregulated interleukin (IL)-1 receptor-associated kinase and TNF receptor-associated factor 6, two key adaptor/scaffold proteins in the IL-1 and Toll-like receptor signaling pathway, known to positively regulate NF-kappaB activity. Impaired NF-kappaB activity was evident from reduced phosphorylation of the NF-kappaB inhibitor IkappaBalpha, reduced NF-kappaB DNA-binding activity and suppressed expression of the NF-kappaB target genes IL-8, IL-6 and matrix metalloproteinase-9. Functionally, miR-146a/b-expressing MDA-MB-231 cells showed markedly impaired invasion and migration capacity relative to control cells. These findings implicate miR-146a/b as a negative regulator of constitutive NF-kappaB activity in a breast cancer setting and suggest that modulating miR-146a/b levels has therapeutic potential to suppress breast cancer metastases.Oncogene advance online publication, 26 May 2008; doi:10.1038/onc.2008.171.
LinkOut: [PMID: 18504431]