Accession ID: MIRT004436 [miRNA, hsa-miR-155 :: CTLA4, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-155 LinkOut: [miRBase ]
Description Homo sapiens miR-155 stem-loop
Comment Human mir-155 is predicted based on homology to a cloned miR from mouse (MIR:MI0000177) .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-155-3p
Mature Sequence 43| CUCCUACAUAUUAGCAUUAACA |64
Evidence Experimental
Experiments Cloned
Putative hsa-miR-155-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-155-5p
Mature Sequence 4| UUAAUGCUAAUCGUGAUAGGGGU |26
Evidence Experimental
Experiments Cloned
Putative hsa-miR-155-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol CTLA4 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, ICOS, IDDM12
Description cytotoxic T-lymphocyte-associated protein 4
Transcript NM_001037631   LinkOut: [ RefSeq ]
Other Transcripts NM_005214   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04514    Cell adhesion molecules (CAMs) - Homo sapiens (human)
hsa04660    T cell receptor signaling pathway - Homo sapiens (human)
hsa05320    Autoimmune thyroid disease - Homo sapiens (human)
Putative miRNA Targets on CTLA4 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of CTLA4
(miRNA target sites are highlighted)		 
>CTLA4|NM_001037631|3'UTR
   1 TGAAAAGCAATTTCAGCCTTATTTTATTCCCATCAATTGAGAAACCATTATGAAGAAGAGAGTCCATATTTCAATTTCCA
  81 AGAGCTGAGGCAATTCTAACTTTTTTGCTATCCAGCTATTTTTATTTGTTTGTGCATTTGGGGGGAATTCATCTCTCTTT
 161 AATATAAAGTTGGATGCGGAACCCAAATTACGTGTACTACAATTTAAAGCAAAGGAGTAGAAAGACAGAGCTGGGATGTT
 241 TCTGTCACATCAGCTCCACTTTCAGTGAAAGCATCACTTGGGATTAATATGGGGATGCAGCATTATGATGTGGGTCAAGG
 321 AATTAAGTTAGGGAATGGCACAGCCCAAAGAAGGAAAAGGCAGGGAGCGAGGGAGAAGACTATATTGTACACACCTTATA
 401 TTTACGTATGAGACGTTTATAGCCGAAATGATCTTTTCAAGTTAAATTTTATGCCTTTTATTTCTTAAACAAATGTATGA
 481 TTACATCAAGGCTTCAAAAATACTCACATGGCTATGTTTTAGCCAGTGATGCTAAAGGTTGTATTGCATATATACATATA
 561 TATATATATATATATATATATATATATATATATATATATATATATATATATTTTAATTTGATAGTATTGTGCATAGAGCC
 641 ACGTATGTTTTTGTGTATTTGTTAATGGTTTGAATATAAACACTATATGGCAGTGTCTTTCCACCTTGGGTCCCAGGGAA
 721 GTTTTGTGGAGGAGCTCAGGACACTAATACACCAGGTAGAACACAAGGTCATTTGCTAACTAGCTTGGAAACTGGATGAG
 801 GTCATAGCAGTGCTTGATTGCGTGGAATTGTGCTGAGTTGGTGTTGACATGTGCTTTGGGGCTTTTACACCAGTTCCTTT
 881 CAATGGTTTGCAAGGAAGCCACAGCTGGTGGTATCTGAGTTGACTTGACAGAACACTGTCTTGAAGACAATGGCTTACTC
 961 CAGGAGACCCACAGGTATGACCTTCTAGGAAGCTCCAGTTCGATGGGCCCAATTCTTACAAACATGTGGTTAATGCCATG
1041 GACAGAAGAAGGCAGCAGGTGGCAGAATGGGGTGCATGAAGGTTTCTGAAAATTAACACTGCTTGTGTTTTTAACTCAAT
1121 ATTTTCCATGAAAATGCAACAACATGTATAATATTTTTAATTAAATAAAAATCTGTGGTGGTCGTTTTAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' acaaUUACG----AUUA-UACAUCCuc 5'
              :||||    | || ||||:||  
Target 5' tgggGATGCAGCATTATGATGTGGGtc 3'
 		290 - 316 120.00 -10.72
2 
miRNA  3' acaaUUACGAUUAUACAUCCUc 5'
              :| |:|  |:|| |||| 
Target 5' caggGAAGTT-TTGTGGAGGAg 3'
 		714 - 734 117.00 -11.00
3 
miRNA  3' acaAUUACGAUUAU--ACAUccuc 5'
             |:||||||| :  ||||    
Target 5' cagTGATGCTAAAGGTTGTAttgc 3'
 		524 - 547 109.00 -8.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-155 :: CTLA4    [ Functional MTI ]
Validation Method Microarray
Conditions Human peripheral blood mononuclear cells (PBMC)
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... We used the miRNA target prediction software TargetScan (version 4.2, www.targetscan.org) to scan for ‘‘seed matches’’ the 770 probe sets up-regulated in miR-155 silenced moDCs (Table S4).//{This MTI is shown in Table S4} ...

- Ceppi, M. Pereira, P. M. Dunand-Sauthier, et al., 2009, Proc Natl Acad Sci U S A.
Article - Ceppi, M. Pereira, P. M. Dunand-Sauthier, et al.
- Proc Natl Acad Sci U S A, 2009
In response to inflammatory stimulation, dendritic cells (DCs) have a remarkable pattern of differentiation (maturation) that exhibits specific mechanisms to control immunity. Here, we show that in response to Lipopolysaccharides (LPS), several microRNAs (miRNAs) are regulated in human monocyte-derived dendritic cells. Among these miRNAs, miR-155 is highly up-regulated during maturation. Using LNA silencing combined to microarray technology, we have identified the Toll-like receptor/interleukin-1 (TLR/IL-1) inflammatory pathway as a general target of miR-155. We further demonstrate that miR-155 directly controls the level of TAB2, an important signal transduction molecule. Our observations suggest, therefore, that in mature human DCs, miR-155 is part of a negative feedback loop, which down-modulates inflammatory cytokine production in response to microbial stimuli.
LinkOut: [PMID: 19193853]