Accession ID: MIRT004559 [miRNA, hsa-miR-296-5p :: WNK4, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-296LinkOut: [miRBase ]
Description Homo sapiens miR-296 stem-loop
Comment This sequence is the predicted human homologue of mouse miR-296 cloned from mouse embryonic stem cells .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-296-5p
Evidence Experimental
Experiments Cloned
Putative hsa-miR-296-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Transcript    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of
(miRNA target sites are highlighted)
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-296-5p :: WNK4    [ Functional MTI ]
Validation Method Luciferase reporter assay , qRT-PCR , Western blot , Other
Conditions HEK293
Location of target site 3'UTR
Original Description (Extracted from the article) ... hWNK4 3 -UTR was the direct target of miR-296 ...

- Mao, J. Li, C. Zhang, Y. Li, Y. Zhao, Y., 2010, Int J Biochem Cell Biol.

Article - Mao, J. Li, C. Zhang, Y. Li, Y. Zhao, Y.
- Int J Biochem Cell Biol, 2010
Human with-no-lysine kinase-4 (hWNK4) is a member of the serine-threonine protein kinase family and may be involved in pathophysiological processes of hypertension through regulating diverse ion transporters. We are interested in the molecular mechanism of regulating hWNK4 expression. The 3'-untranslated region (3'-UTR) is an important area for gene regulation. Here, we focused on the 3'-UTR of hWNK4 to investigate how it regulates hWNK4 expression. A luciferase assay showed that the hWNK4 3'-UTR obviously enhanced the transcriptional activity of the promoter, whether homologous or heterologous. Chromosome conformation capture assay further demonstrated that the 3'-UTR enhancer functioned through distant crosstalking with the hWNK4 promoter in a cell-specific manner. Following the deletion of the promoter, the enhancer action of the hWNK4 3'-UTR fell away, indicating that the motifs within the promoter could be essential for the function of the hWNK4 3'-UTR. The hWNK4 3'-UTR, meanwhile, contained a miR-296 binding site, predicted by bioinformatics algorithms and verified as a true target using a reporter assay. Expression of hWNK4 can be downregulated by miR-296 at the posttranscriptional level in cell-specific pattern, shown by real-time quantitative PCR and Western blot assays. These data demonstrate the hWNK4 3'-UTR plays an enhancer role by crosstalking with the promoter, and miR-296 suppresses hWNK4 expression through targeting on its 3'-UTR in a cell-specific fashion. The coordinated modulation of miR-296 and cofactors on the 3'-UTR may be attributed to hWNK4 physiopathological function.
LinkOut: [PMID: 20561597]
MiRNA-Target Expression Profile:

MiRNA-Target Expression Profile(TCGA):

MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
59 hsa-miR-296-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004559 WNK4 WNK lysine deficient protein kinase 4 4 1
MIRT004900 HGS hepatocyte growth factor-regulated tyrosine kinase substrate 3 1
MIRT006438 SCRIB scribbled homolog (Drosophila) 1 1
MIRT006572 IKBKE inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon 1 1
MIRT006683 BBC3 BCL2 binding component 3 3 1
MIRT035568 CCND1 cyclin D1 1 1
MIRT035569 ABCB1 ATP-binding cassette, sub-family B (MDR/TAP), member 1 1 1
MIRT035570 BAX BCL2-associated X protein 1 1
MIRT035571 CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) 1 1
MIRT035572 BCL2 B-cell CLL/lymphoma 2 1 1
MIRT054157 CDX1 caudal type homeobox 1 4 1
MIRT069026 KCTD12 potassium channel tetramerisation domain containing 12 1 2
MIRT132295 ARL8A ADP-ribosylation factor-like 8A 1 1
MIRT248249 SP1 Sp1 transcription factor 1 1
MIRT256751 RNF44 ring finger protein 44 1 1
MIRT272486 LUZP1 leucine zipper protein 1 1 3
MIRT438126 PIN1 peptidylprolyl cis/trans isomerase, NIMA-interacting 1 1 1
MIRT438127 PIN1 peptidylprolyl cis/trans isomerase, NIMA-interacting 1 1 1
MIRT438128 PIN1 peptidylprolyl cis/trans isomerase, NIMA-interacting 1 1 1
MIRT453073 SUMF2 sulfatase modifying factor 2 1 3
MIRT462501 MTFMT mitochondrial methionyl-tRNA formyltransferase 1 4
MIRT472742 MTSS1L metastasis suppressor 1-like 1 1
MIRT478964 COPS7B COP9 constitutive photomorphogenic homolog subunit 7B (Arabidopsis) 1 1
MIRT483565 SYT2 synaptotagmin II 1 1
MIRT488230 DNLZ DNL-type zinc finger 1 1
MIRT488536 VAMP1 vesicle-associated membrane protein 1 (synaptobrevin 1) 1 1
MIRT489962 GNB2 guanine nucleotide binding protein (G protein), beta polypeptide 2 1 1
MIRT492184 SOX12 SRY (sex determining region Y)-box 12 1 1
MIRT492320 SETD1B SET domain containing 1B 1 1
MIRT492824 NXN nucleoredoxin 1 2
MIRT495391 VPS51 vacuolar protein sorting 51 homolog (S. cerevisiae) 1 1
MIRT495876 DPY19L1 dpy-19-like 1 (C. elegans) 1 1
MIRT513599 VPS37B vacuolar protein sorting 37 homolog B (S. cerevisiae) 1 1
MIRT528354 ZDHHC15 zinc finger, DHHC-type containing 15 1 1
MIRT528710 S100A2 S100 calcium binding protein A2 1 1
MIRT532729 POLR2E polymerase (RNA) II (DNA directed) polypeptide E, 25kDa 1 1
MIRT533669 TMEM86A transmembrane protein 86A 1 1
MIRT533776 TMEM133 transmembrane protein 133 1 1
MIRT534257 SLC12A7 solute carrier family 12 (potassium/chloride transporters), member 7 1 1
MIRT570603 NFIC nuclear factor I/C (CCAAT-binding transcription factor) 1 1
MIRT610468 ADAMTS13 ADAM metallopeptidase with thrombospondin type 1 motif, 13 1 2
MIRT617174 GOSR2 golgi SNAP receptor complex member 2 1 1
MIRT623187 NAA15 N(alpha)-acetyltransferase 15, NatA auxiliary subunit 1 1
MIRT624037 ELAVL3 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) 1 1
MIRT635308 FAM179A family with sequence similarity 179, member A 1 1
MIRT654977 PLEKHA2 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 2 1 1
MIRT660521 ARL4C ADP-ribosylation factor-like 4C 1 1
MIRT691841 OSCAR osteoclast associated, immunoglobulin-like receptor 1 1
MIRT705282 B4GALT1 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 1 1
MIRT712181 SRSF10 serine/arginine-rich splicing factor 10 1 1
MIRT715905 MFRP membrane frizzled-related protein 1 1
MIRT715917 ACVRL1 activin A receptor type II-like 1 1 1
MIRT716262 TOM1 target of myb1 (chicken) 1 1
MIRT718192 PSMF1 proteasome (prosome, macropain) inhibitor subunit 1 (PI31) 1 1
MIRT718207 CDC42EP4 CDC42 effector protein (Rho GTPase binding) 4 1 1
MIRT718251 ZDHHC8 zinc finger, DHHC-type containing 8 1 1
MIRT719265 GIGYF1 GRB10 interacting GYF protein 1 1 1
MIRT720314 CHCHD4 coiled-coil-helix-coiled-coil-helix domain containing 4 1 1
MIRT722810 SUSD2 sushi domain containing 2 1 1
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