Accession ID: MIRT004563 [miRNA, hsa-miR-92a :: ESR2, target gene]
|Synonyms||ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2|
|Description||estrogen receptor 2 (ER beta)|
|Other Transcripts||NM_001040276, NM_001437|
|Putative miRNA Targets on ESR2|
|3'UTR of ESR2
(miRNA target sites are highlighted)
>ESR2|NM_001040275|3'UTR 1 TGACCCTCTAATCAACTCGGTGGCCTAAAGAAAAATCTTGGGTAACATTTTCACTTCAGTTTCCCTCTGGGATCATTGTA 81 ATCCATGAAAAAAATAATTTTAAAGAAAGAGTTAAAATACTTTGAAGTTAGTTATGTGGTTAAAAACCACCTTCCTTTCT 161 ATTATCAATCCAACAATTTGATAACTGTAAACGCTAAAGTGAAGACGGATTCTCTTCAGATGGTCTCCTTAACTGCCCAG 241 GGCTTGCAGATGTCTCACCCATGAGGGGCACCAATGTAGAAAGCTGAGGCTTCATCTACTGATGAGCTTCACTGGTTTCC 321 CCTGAGGTTTGTGCTTTGGCAGAGAAGGGGAGGAGGGGACTGGGATTGTGTGGTCAGCTGTGCCTGCCAACAGATGCAGG 401 TTAGGAACTGTGTTCAGTATCTTCCAATAAGAAAGGGGAAATGCCGATGCCTATCCTCTTTGTTTAGGTAGAAAGTAAAA 481 TGCTACTGGACTTAAATGGGCAAAAAAAAAAAAAAAA
|miRNA:Target||hsa-miR-92a :: ESR2 [ Functional MTI ]|
|Validation Method||GFP reporter assay|
|Location of target site||3'UTR|
|Tools used in this research||mirGen|
|Original Description (Extracted from the article)||... ERI21 is targeted by miR-92 via its 3'-UTR ...
- Al-Nakhle, H. Burns, P. A. Cummings, M. et al., 2010, Cancer Res.
- Cancer Res, 2010
Estrogen receptor beta1 (ERbeta1) downregulation occurs in many breast cancers, but the responsible molecular mechanisms remain unclear. Here, we report that levels of ERbeta1 expression are negatively regulated by the microRNA miR-92. Expression analysis in a cohort of primary breast tumors confirmed a significant negative correlation between miR-92 and both ERbeta1 mRNA and protein. Inhibition of miR-92 in MCF-7 cells increased ERbeta1 expression in a dose-dependent manner, whereas miR-92 overexpression led to ERbeta1 downregulation. Reporter constructs containing candidate miR-92 binding sites in the 3'-untranslated region (UTR) of ERbeta1 suggested by bioinformatics analysis confirmed that miR-92 downregulated ERbeta1 via direct targeting of its 3'-UTR. Our results define a potentially important mechanism for downregulation of ERbeta1 expression in breast cancer.LinkOut: [PMID: 20484043]