Accession ID: MIRT004563 [miRNA, hsa-miR-92a :: ESR2, target gene]
miRNA Infomation
miRNA namehsa-miR-92a
miRNA-target interaction network
Gene Information
Gene Symbol ESR2 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2
Description estrogen receptor 2 (ER beta)
Transcript NM_001040275   LinkOut: [ RefSeq ]
Other Transcripts NM_001040276, NM_001437   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on ESR2 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of ESR2
(miRNA target sites are highlighted)		 
>ESR2|NM_001040275|3'UTR
   1 TGACCCTCTAATCAACTCGGTGGCCTAAAGAAAAATCTTGGGTAACATTTTCACTTCAGTTTCCCTCTGGGATCATTGTA
  81 ATCCATGAAAAAAATAATTTTAAAGAAAGAGTTAAAATACTTTGAAGTTAGTTATGTGGTTAAAAACCACCTTCCTTTCT
 161 ATTATCAATCCAACAATTTGATAACTGTAAACGCTAAAGTGAAGACGGATTCTCTTCAGATGGTCTCCTTAACTGCCCAG
 241 GGCTTGCAGATGTCTCACCCATGAGGGGCACCAATGTAGAAAGCTGAGGCTTCATCTACTGATGAGCTTCACTGGTTTCC
 321 CCTGAGGTTTGTGCTTTGGCAGAGAAGGGGAGGAGGGGACTGGGATTGTGTGGTCAGCTGTGCCTGCCAACAGATGCAGG
 401 TTAGGAACTGTGTTCAGTATCTTCCAATAAGAAAGGGGAAATGCCGATGCCTATCCTCTTTGTTTAGGTAGAAAGTAAAA
 481 TGCTACTGGACTTAAATGGGCAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-92a :: ESR2    [ Functional MTI ]
Validation Method GFP reporter assay
Conditions MCF-7
Location of target site 3'UTR
Tools used in this research mirGen
Original Description (Extracted from the article) ... ERI21 is targeted by miR-92 via its 3'-UTR ...

- Al-Nakhle, H. Burns, P. A. Cummings, M. et al., 2010, Cancer Res.
Article - Al-Nakhle, H. Burns, P. A. Cummings, M. et al.
- Cancer Res, 2010
Estrogen receptor beta1 (ERbeta1) downregulation occurs in many breast cancers, but the responsible molecular mechanisms remain unclear. Here, we report that levels of ERbeta1 expression are negatively regulated by the microRNA miR-92. Expression analysis in a cohort of primary breast tumors confirmed a significant negative correlation between miR-92 and both ERbeta1 mRNA and protein. Inhibition of miR-92 in MCF-7 cells increased ERbeta1 expression in a dose-dependent manner, whereas miR-92 overexpression led to ERbeta1 downregulation. Reporter constructs containing candidate miR-92 binding sites in the 3'-untranslated region (UTR) of ERbeta1 suggested by bioinformatics analysis confirmed that miR-92 downregulated ERbeta1 via direct targeting of its 3'-UTR. Our results define a potentially important mechanism for downregulation of ERbeta1 expression in breast cancer.
LinkOut: [PMID: 20484043]