Accession ID: MIRT004597 [miRNA, hsa-miR-31-5p :: CASR, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-31LinkOut: [miRBase ]
Synonyms MIRN31, hsa-mir-31, miR-31, MIR31
Description Homo sapiens miR-31 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations.
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-31-5p
Mature Sequence 8| AGGCAAGAUGCUGGCAUAGCU |28
Evidence Experimental
Experiments Cloned
Putative hsa-miR-31-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol CASR LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms CAR, EIG8, FHH, FIH, GPRC2A, HHC, HHC1, MGC138441, NSHPT, PCAR1
Description calcium-sensing receptor
Transcript NM_0003    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on CASR LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of CASR
(miRNA target sites are highlighted)
>CASR|NM_0003|3'UTR
   1 TAAAATGGAAGGAGAAGACTGGGCTAGGGAGAATGCAGAGAGGTTTCTTGGGGTCCCAGGGAAGAGGAATCGCCCCAGAC
  81 TCCTTTCCTCTGAGGAAGAAGGGATAATAGACACATCAAATGCCCCGAATTTAGTCACACCATCTTAAATGACAGTGAAT
 161 TGACCCATGTTCCCTTTAAAATTAAAAAAAAGAAGAGCCTTGTGTTTCTGTGGTTGCATTTGTCAAAGCATTGAGATCTC
 241 CACGGTCAGATTTGCTGTTCACCCACATCTAATGTCTCTTCCTCTGTTCTATCCCACCCAACAGCTCAGAGATGAAACTA
 321 TGGCTTTAAACTACCCTCCAGAGTGTGCAGACTGATGGGACATCAAATTTGCCACCACTAGAGCTGAGAGTCTGAAAGAC
 401 AGAATGTCACCAGTCCTGCCCAATGCCTTGACAACAGACTGAATTTTAAATGTTCACAACATAAGGAGAATGTATCTCCT
 481 CCTATTTATGAAAACCATATGATATTTTGTCTCCTACCTGCTGCTGCTATTATGTAACATCCAGAAGGTTTGCACCCCTC
 561 CTATACCATATGTCTGCTTCTGTCCAGGACATGATACTGATGCCATGTTTAGATTCCAGGATCACAAGAATCACCTCAAA
 641 TTGTTAGGAAGGGACTGCATAAACCAATGAGCTGTATCTGTAATTAATATTCCTATATGTAGCTTTATCCTTAGGAAAAT
 721 GCTTCTGTTGTAATAGTCCATGGACAATATAAACTGAAAAATGTCAGTCTGGTTTATATAAGGCAGTATTATTGAGCTCT
 801 ATTTCCCCACCCCACTATCCTCACTCCCATAAGCTAAGCCTTATGTGAGCCCCTTCAGGGACTCAAGGGTCCAGAAGTCC
 881 CTCCCATCTCTACCCCAAAGAATTCCTGAAGCCAGATCCACCCTATCCCTGTACAGAGTAAGTTCTCAATTATTGGCCTG
 961 CTAATAGCTGCTAGGGTAGGAAAGCGTGGTTCCAAGAAAGATCCACCCTCAAATGTCAGAGCTATGTTCCCTCCAGCAGT
1041 GGTATTAATACTGCCGGTCACCCAGGCTCTGGAGCCAGAGAGACAGACCGGGGTTCAAGCCATGGCTTCGTCATTTGCAA
1121 GCTGAGTGACTGTAGGCAGGGAACCTTAACCTCTCTAAGCCACAGCTTCTTCATCTTTAAAATAAGGATAATAATCATTC
1201 TTTCCCCTCAGAGCTCTTATGTGGATTAAACGAGATAATGTATATAAAGTACTTTAGCCTGGTACCTAGCACACAATAAG
1281 CATTCAATAAATATTAGTTAATATTATTAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' ucgaUACGGUCGU-AGAACGGa 5'
              |||:|| || || |||| 
Target 5' cagaATGTCACCAGTCCTGCCc 3'
400 - 421 133.00 -12.40
2
miRNA  3' ucGAUAC----GGU---C-GUAGAACGGa 5'
            :||||    |||   | :||:|||:| 
Target 5' atTTATGAAAACCATATGATATTTTGTCt 3'
484 - 512 121.00 -10.20
3
miRNA  3' ucGAUACG--GUCGUAGAAcgga 5'
            ||| ||  |||| ||||    
Target 5' ctCTAAGCCACAGCTTCTTcatc 3'
1153 - 1175 109.00 -10.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-31-5p :: CASR    [ Functional MTI ]
Validation Method LacZ assay , Other
Conditions SAS
Location of target site 3'UTR
Tools used in this research PicTar , TargetScan
Original Description (Extracted from the article) ... miR-31 expression repressed FIH expression and mutations within the predictive miR-31 target site in the FIH 3' UTR abrogated FIH repression ...

- Liu, C. J. Tsai, M. M. Hung, P. S. Kao, S. et al., 2010, Cancer Res.

Article - Liu, C. J. Tsai, M. M. Hung, P. S. Kao, S. et al.
- Cancer Res, 2010
MicroRNAs (miRNA) are endogenously expressed noncoding RNAs with important biological and pathological functions that are yet to be fully defined. This study investigated alterations in miRNA expression in head and neck squamous cell carcinoma (HNSCC), the incidence of which is rising throughout the world. Initial screening and subsequent analysis identified a panel of aberrantly expressed miRNAs in HNSCC tissues, with miR-31 among the most markedly upregulated. Ectopic expression of miR-31 increased the oncogenic potential of HNSCC cells under normoxic conditions in cell culture or tumor xenografts. Conversely, blocking miR-31 expression reduced the growth of tumor xenografts. The in silico analysis suggested that miR-31 may target the 3' untranslated region (UTR) of factor-inhibiting hypoxia-inducible factor (FIH), a hypoxia-inducible factor (HIF) regulatory factor that inhibits the ability of HIF to act as a transcriptional regulator under normoxic conditions. In support of this likelihood, miR-31 expression repressed FIH expression and mutations within the predictive miR-31 target site in the FIH 3' UTR abrogated FIH repression. Furthermore, miR-31 expression increased HIF transactivation activity. We found that FIH suppressed oncogenic phenotypes under normoxic conditions and that this activity was abrogated by functional mutations. Lastly, increased miR-31 expression was correlated with decreased levels of FIH in tumor tissues. Our findings suggest that miR-31 contributes to the development of HNSCC by impeding FIH to activate HIF under normoxic conditions.
LinkOut: [PMID: 20145132]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
88 hsa-miR-31-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000088 RHOA ras homolog gene family, member A 4 3
MIRT000490 PPP2R2A protein phosphatase 2, regulatory subunit B, alpha 3 1
MIRT000491 LATS2 LATS, large tumor suppressor, homolog 2 (Drosophila) 3 1
MIRT001180 FOXP3 forkhead box P3 4 1
MIRT004019 SELE selectin E 3 1
MIRT004597 CASR calcium-sensing receptor 1 1
MIRT004968 YY1 YY1 transcription factor 3 1
MIRT004969 RET ret proto-oncogene 3 1
MIRT004970 NUMB numb homolog (Drosophila) 3 1
MIRT004971 NFAT5 nuclear factor of activated T-cells 5, tonicity-responsive 3 1
MIRT004972 KLF13 Kruppel-like factor 13 3 1
MIRT004973 JAZF1 JAZF zinc finger 1 3 1
MIRT004974 HOXC13 homeobox C13 3 1
MIRT004975 ETS1 v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) 3 1
MIRT004976 ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) 4 1
MIRT004977 MPRIP myosin phosphatase Rho interacting protein 3 1
MIRT004978 MMP16 matrix metallopeptidase 16 (membrane-inserted) 4 1
MIRT004979 RDX radixin 4 1
MIRT004980 CXCL12 chemokine (C-X-C motif) ligand 12 3 1
MIRT004981 ARPC5 actin related protein 2/3 complex, subunit 5, 16kDa 3 1
MIRT004984 FZD3 frizzled homolog 3 (Drosophila) 4 1
MIRT005456 DMD dystrophin 4 1
MIRT005566 TIAM1 T-cell lymphoma invasion and metastasis 1 4 1
MIRT005707 ICAM1 intercellular adhesion molecule 1 1 1
MIRT005874 DKK1 dickkopf homolog 1 (Xenopus laevis) 4 1
MIRT005875 DACT3 dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) 4 1
MIRT006026 WASF3 WAS protein family, member 3 2 1
MIRT006468 HIF1AN hypoxia inducible factor 1, alpha subunit inhibitor 3 2
MIRT006567 SATB2 SATB homeobox 2 1 1
MIRT007286 PRKCE protein kinase C, epsilon 1 1
MIRT007292 RASA1 RAS p21 protein activator (GTPase activating protein) 1 3 1
MIRT007319 STK40 serine/threonine kinase 40 2 2
MIRT007327 MCM2 minichromosome maintenance complex component 2 1 1
MIRT007328 CDK1 cyclin-dependent kinase 1 1 1
MIRT007367 CREG1 cellular repressor of E1A-stimulated genes 1 3 1
MIRT028387 Lats2 large tumor suppressor 2 1 1
MIRT035519 MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) 1 1
MIRT035528 MET met proto-oncogene (hepatocyte growth factor receptor) 1 2
MIRT049857 PPIL2 peptidylprolyl isomerase (cyclophilin)-like 2 1 1
MIRT049858 ZC3H18 zinc finger CCCH-type containing 18 1 1
MIRT049859 RPS7 ribosomal protein S7 1 1
MIRT049860 MED12 mediator complex subunit 12 1 1
MIRT049861 CYP27B1 cytochrome P450, family 27, subfamily B, polypeptide 1 1 1
MIRT049862 HIST1H2BC histone cluster 1, H2bc 1 1
MIRT049863 PTPRJ protein tyrosine phosphatase, receptor type, J 1 1
MIRT049864 AGO1 eukaryotic translation initiation factor 2C, 1 1 1
MIRT049865 HEATR2 HEAT repeat containing 2 1 1
MIRT049866 HIST1H2BK histone cluster 1, H2bk 1 1
MIRT049867 RPA1 replication protein A1, 70kDa 1 1
MIRT049868 TXNDC5 thioredoxin domain containing 5 (endoplasmic reticulum) 1 1
MIRT049869 AKAP8L A kinase (PRKA) anchor protein 8-like 1 1
MIRT049870 FLNA filamin A, alpha 1 1
MIRT049871 ATP2A2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 1 1
MIRT049872 NDFIP2 Nedd4 family interacting protein 2 1 1
MIRT049873 TRIB3 tribbles homolog 3 (Drosophila) 1 1
MIRT049874 NUP188 nucleoporin 188kDa 1 1
MIRT049875 RPS4Y1 ribosomal protein S4, Y-linked 1 1 1
MIRT049876 GLI2 GLI family zinc finger 2 1 1
MIRT049877 RPL37A ribosomal protein L37a 1 1
MIRT049878 EXOSC5 exosome component 5 1 1
MIRT049879 DDX19A DEAD (Asp-Glu-Ala-As) box polypeptide 19A 1 1
MIRT049880 UBA6 ubiquitin-like modifier activating enzyme 6 1 1
MIRT049881 NOL9 nucleolar protein 9 1 1
MIRT049882 DNAJC5 DnaJ (Hsp40) homolog, subfamily C, member 5 1 1
MIRT049883 FAM134C family with sequence similarity 134, member C 1 1
MIRT049884 PEX19 peroxisomal biogenesis factor 19 1 1
MIRT049885 SMEK2 SMEK homolog 2, suppressor of mek1 (Dictyostelium) 1 1
MIRT049886 TRRAP transformation/transcription domain-associated protein 1 1
MIRT049887 RANGAP1 Ran GTPase activating protein 1 1 1
MIRT049888 TNRC6B trinucleotide repeat containing 6B 1 1
MIRT049889 NOP56 NOP56 ribonucleoprotein homolog (yeast) 1 1
MIRT049890 PCSK1N proprotein convertase subtilisin/kexin type 1 inhibitor 1 1
MIRT049891 ZNF275 zinc finger protein 275 1 1
MIRT049892 C2CD5 KIAA0528 1 1
MIRT049893 NFATC2IP nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein 1 1
MIRT049894 HIST1H2BJ histone cluster 1, H2bj 1 1
MIRT049895 SRRM2 serine/arginine repetitive matrix 2 1 1
MIRT049896 GYG1 glycogenin 1 1 1
MIRT049897 ZC3H12C zinc finger CCCH-type containing 12C 1 1
MIRT049898 LRRC59 leucine rich repeat containing 59 1 1
MIRT049899 GHITM growth hormone inducible transmembrane protein 1 1
MIRT049900 EDC3 enhancer of mRNA decapping 3 homolog (S. cerevisiae) 1 1
MIRT049901 MYO1D myosin ID 1 1
MIRT049902 STOML2 stomatin (EPB72)-like 2 1 1
MIRT049903 EXOC6 exocyst complex component 6 1 1
MIRT049904 HOXA7 homeobox A7 1 1
MIRT049905 SFXN1 sideroflexin 1 1 1
MIRT049906 ILF2 interleukin enhancer binding factor 2, 45kDa 1 1