Accession ID: MIRT004701 [miRNA, hsa-miR-185 :: AKT1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-185 LinkOut: [miRBase ]
Synonyms MIRN185, miR-185, MIR185
Description Homo sapiens miR-185 stem-loop
Comment This miRNA sequence is predicted based on homology to a verified miRNA from mouse .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-185-3p
Mature Sequence 50| AGGGGCUGGCUUUCCUCUGGUC |71
Evidence Experimental
Experiments Cloned
Putative hsa-miR-185-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-185-5p
Mature Sequence 15| UGGAGAGAAAGGCAGUUCCUGA |36
Evidence Experimental
Experiments Cloned
Putative hsa-miR-185-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol AKT1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Description v-akt murine thymoma viral oncogene homolog 1
Transcript NM_005163   LinkOut: [ RefSeq ]
Other Transcripts NM_001014432, NM_001014431   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04010    MAPK signaling pathway - Homo sapiens (human)
hsa04012    ErbB signaling pathway - Homo sapiens (human)
hsa04062    Chemokine signaling pathway - Homo sapiens (human)
hsa04150    mTOR signaling pathway - Homo sapiens (human)
hsa04210    Apoptosis - Homo sapiens (human)
hsa04370    VEGF signaling pathway - Homo sapiens (human)
hsa04510    Focal adhesion - Homo sapiens (human)
hsa04530    Tight junction - Homo sapiens (human)
hsa04620    Toll-like receptor signaling pathway - Homo sapiens (human)
hsa04630    Jak-STAT signaling pathway - Homo sapiens (human)
hsa04660    T cell receptor signaling pathway - Homo sapiens (human)
hsa04662    B cell receptor signaling pathway - Homo sapiens (human)
hsa04664    Fc epsilon RI signaling pathway - Homo sapiens (human)
hsa04666    Fc gamma R-mediated phagocytosis - Homo sapiens (human)
hsa04722    Neurotrophin signaling pathway - Homo sapiens (human)
hsa04910    Insulin signaling pathway - Homo sapiens (human)
hsa04914    Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa04920    Adipocytokine signaling pathway - Homo sapiens (human)
hsa05142    Chagas disease - Homo sapiens (human)
hsa05200    Pathways in cancer - Homo sapiens (human)
hsa05210    Colorectal cancer - Homo sapiens (human)
hsa05211    Renal cell carcinoma - Homo sapiens (human)
hsa05212    Pancreatic cancer - Homo sapiens (human)
hsa05213    Endometrial cancer - Homo sapiens (human)
hsa05214    Glioma - Homo sapiens (human)
hsa05215    Prostate cancer - Homo sapiens (human)
hsa05218    Melanoma - Homo sapiens (human)
hsa05220    Chronic myeloid leukemia - Homo sapiens (human)
hsa05221    Acute myeloid leukemia - Homo sapiens (human)
hsa05222    Small cell lung cancer - Homo sapiens (human)
hsa05223    Non-small cell lung cancer - Homo sapiens (human)
Putative miRNA Targets on AKT1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of AKT1
(miRNA target sites are highlighted)		 
>AKT1|NM_005163|3'UTR
   1 TCGGCCAGCGGCACGGCCTGAGGCGGCGGTGGACTGCGCTGGACGATAGCTTGGAGGGATGGAGAGGCGGCCTCGTGCCA
  81 TGATCTGTATTTAATGGTTTTTATTTCTCGGGTGCATTTGAGAGAAGCCACGCTGTCCTCTCGAGCCCAGATGGAAAGAC
 161 GTTTTTGTGCTGTGGGCAGCACCCTCCCCCGCAGCGGGGTAGGGAAGAAAACTATCCTGCGGGTTTTAATTTATTTCATC
 241 CAGTTTGTTCTCCGGGTGTGGCCTCAGCCCTCAGAACAATCCGATTCACGTAGGGAAATGTTAAGGACTTCTGCAGCTAT
 321 GCGCAATGTGGCATTGGGGGGCCGGGCAGGTCCTGCCCATGTGTCCCCTCACTCTGTCAGCCAGCCGCCCTGGGCTGTCT
 401 GTCACCAGCTATCTGTCATCTCTCTGGGGCCCTGGGCCTCAGTTCAACCTGGTGGCACCAGATGCAACCTCACTATGGTA
 481 TGCTGGCCAGCACCCTCTCCTGGGGGTGGCAGGCACACAGCAGCCCCCCAGCACTAAGGCCGTGTCTCTGAGGACGTCAT
 561 CGGAGGCTGGGCCCCTGGGATGGGACCAGGGATGGGGGATGGGCCAGGGTTTACCCAGTGGGACAGAGGAGCAAGGTTTA
 641 AATTTGTTATTGTGTATTATGTTGTTCAAATGCATTTTGGGGGTTTTTAATCTTTGTGACAGGAAAGCCCTCCCCCTTCC
 721 CCTTCTGTGTCACAGTTCTTGGTGACTGTCCCACCGGGAGCCTCCCCCTCAGATGATCTCTCCACGGTAGCACTTGACCT
 801 TTTCGACGCTTAACCTTTCCGCTGTCGCCCCAGGCCCTCCCTGACTCCCTGTGGGGGTGGCCATCCCTGGGCCCCTCCAC
 881 GCCTCCTGGCCAGACGCTGCCGCTGCCGCTGCACCACGGCGTTTTTTTACAACATTCAACTTTAGTATTTTTACTATTAT
 961 AATATAATATGGAACCTTCCCTCCAAATTCTTCAATAAAAGTTGCTTTTCAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' cuggUCUCCUUUCG------GUCGGGGa 5'
              :| || |:||      ||||||| 
Target 5' tgggGGTGGCAGGCACACAGCAGCCCCc 3'
 		501 - 528 148.00 -19.20
2 
miRNA  3' cuGGUCUCCUUUCG-GUCGGGGa 5'
            ||:|: | :: | ||||||: 
Target 5' ctCCGGGTGTGGCCTCAGCCCTc 3'
 		250 - 272 136.00 -19.00
3 
miRNA  3' cuggUCU-CCUUUCGGUCGGGGa 5'
              :|| |||:|| |:|||:| 
Target 5' ggagGGATGGAGAGGCGGCCTCg 3'
 		53 - 75 134.00 -22.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-185 :: AKT1    [ Functional MTI ]
Validation Method Flow , Immunoblot , Microarray , qRT-PCR
Conditions H1299, A549
Location of target site 3'UTR
Tools used in this research PicTar, TargetScan
Original Description (Extracted from the article) ... Using miRNA-target prediction analyses and the array data, we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6. ...

- Takahashi, Y. Forrest, A. R. Maeno, E. et al., 2009, PLoS One.
miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1 
miRNA  3' aguccuugacggaaAGAGAGGu 5'
                        ||||||| 
Target 5' ucccccucagaugaUCUCUCCa 3'
 		2 - 23
Article - Takahashi, Y. Forrest, A. R. Maeno, E. et al.
- PLoS One, 2009
BACKGROUND: MicroRNAs (miRNAs) are short single stranded noncoding RNAs that suppress gene expression through either translational repression or degradation of target mRNAs. The annealing between messenger RNAs and 5' seed region of miRNAs is believed to be essential for the specific suppression of target gene expression. One miRNA can have several hundred different targets in a cell. Rapidly accumulating evidence suggests that many miRNAs are involved in cell cycle regulation and consequentially play critical roles in carcinogenesis. METHODOLOGY/PRINCIPAL FINDINGS: Introduction of synthetic miR-107 or miR-185 suppressed growth of the human non-small cell lung cancer cell lines. Flow cytometry analysis revealed these miRNAs induce a G1 cell cycle arrest in H1299 cells and the suppression of cell cycle progression is stronger than that by Let-7 miRNA. By the gene expression analyses with oligonucleotide microarrays, we find hundreds of genes are affected by transfection of these miRNAs. Using miRNA-target prediction analyses and the array data, we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6. CONCLUSIONS/SIGNIFICANCE: We identified new cell cycle regulating miRNAs, miR-107 and miR-185, localized in frequently altered chromosomal regions in human lung cancers. Especially for miR-107, a large number of down-regulated genes are annotated with the gene ontology term 'cell cycle'. Our results suggest that these miRNAs may contribute to regulate cell cycle in human malignant tumors.
LinkOut: [PMID: 19688090]