Accession ID: MIRT004969 [miRNA, hsa-miR-31-5p :: RET, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-31LinkOut: [miRBase ]
Synonyms MIRN31, hsa-mir-31, miR-31, MIR31
Description Homo sapiens miR-31 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations.
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-31-5p
Mature Sequence 8| AGGCAAGAUGCUGGCAUAGCU |28
Evidence Experimental
Experiments Cloned
Putative hsa-miR-31-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol RET LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1, RET51
Description ret proto-oncogene
Transcript NM_020975    LinkOut: [ RefSeq ]
Other Transcripts NM_020630   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on RET LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of RET
(miRNA target sites are highlighted)
>RET|NM_020975|3'UTR
   1 CATTTCTTTGTGAAAGGTAATGGACTCACAAGGGGAAGAAACATGCTGAGAATGGAAAGTCTACCGGCCCTTTCTTTGTG
  81 AACGTCACATTGGCCGAGCCGTGTTCAGTTCCCAGGTGGCAGACTCGTTTTTGGTAGTTTGTTTTAACTTCCAAGGTGGT
 161 TTTACTTCTGATAGCCGGTGATTTTCCCTCCTAGCAGACATGCCACACCGGGTAAGAGCTCTGAGTCTTAGTGGTTAAGC
 241 ATTCCTTTCTCTTCAGTGCCCAGCAGCACCCAGTGTTGGTCTGTGTCCATCAGTGACCACCAACATTCTGTGTTCACATG
 321 TGTGGGTCCAACACTTACTACCTGGTGTATGAAATTGGACCTGAACTGTTGGATTTTTCTAGTTGCCGCCAAACAAGGCA
 401 AAAAAATTTAAACATGAAGCACACACACAAAAAAGGCAGTAGGAAAAATGCTGGCCCTGATGACCTGTCCTTATTCAGAA
 481 TGAGAGACTGCGGGGGGGGCCTGGGGGTAGTGTCAATGCCCCTCCAGGGCTGGAGGGGAAGAGGGGCCCCGAGGATGGGC
 561 CTGGGCTCAGCATTCGAGATCTTGAGAATGATTTTTTTTTAATCATGCAACCTTTCCTTAGGAAGACATTTGGTTTTCAT
 641 CATGATTAAGATGATTCCTAGATTTAGCACAATGGAGAGATTCCATGCCATCTTTACTATGTGGATGGTGGTATCAGGGA
 721 AGAGGGCTCACAAGACACATTTGTCCCCCGGGCCCACCACATCATCCTCACGTGTTCGGTACTGAGCAGCCACTACCCCT
 801 GATGAGAACAGTATGAAGAAAGGGGGCTGTTGGAGTCCCAGAATTGCTGACAGCAGAGGCTTTGCTGCTGTGAATCCCAC
 881 CTGCCACCAGCCTGCAGCACACCCCACAGCCAAGTAGAGGCGAAAGCAGTGGCTCATCCTACCTGTTAGGAGCAGGTAGG
 961 GCTTGTACTCACTTTAATTTGAATCTTATCAACTTACTCATAAAGGGACAGGCTAGCTAGCTGTGTTAGAAGTAGCAATG
1041 ACAATGACCAAGGACTGCTACACCTCTGATTACAATTCTGATGTGAAAAAGATGGTGTTTGGCTCTTATAGAGCCTGTGT
1121 GAAAGGCCCATGGATCAGCTCTTCCTGTGTTTGTAATTTAATGCTGCTACAAGATGTTTCTGTTTCTTAGATTCTGACCA
1201 TGACTCATAAGCTTCTTGTCATTCTTCATTGCTTGTTTGTGGTCACAGATGCACAACACTCCTCCAGTCTTGTGGGGGCA
1281 GCTTTTGGGAAGTCTCAGCAGCTCTTCTGGCTGTGTTGTCAGCACTGTAACTTCGCAGAAAAGAGTCGGATTACCAAAAC
1361 ACTGCCTGCTCTTCAGACTTAAAGCACTGATAGGACTTAAAATAGTCTCATTCAAATACTGTATTTTATATAGGCATTTC
1441 ACAAAAACAGCAAAATTGTGGCATTTTGTGAGGCCAAGGCTTGGATGCGTGTGTAATAGAGCCTTGTGGTGTGTGCGCAC
1521 ACACCCAGAGGGAGAGTTTGAAAAATGCTTATTGGACACGTAACCTGGCTCTAATTTGGGCTGTTTTTCAGATACACTGT
1601 GATAAGTTCTTTTACAAATATCTATAGACATGGTAAACTTTTGGTTTTCAGATATGCTTAATGATAGTCTTACTAAATGC
1681 AGAAATAAGAATAAACTTTCTCAAATTATTAAAAATGCCTACACAGTAAGTGTGAATTGCTGCAACAGGTTTGTTCTCAG
1761 GAGGGTAAGAACTCCAGGTCTAAACAGCTGACCCAGTGATGGGGAATTTATCCTTGACCAATTTATCCTTGACCAATAAC
1841 CTAATTGTCTATTCCTGAGTTATAAAAGTCCCCATCCTTATTAGCTCTACTGGAATTTTCATACACGTAAATGCAGAAGT
1921 TACTAAGTATTAAGTATTACTGAGTATTAAGTAGTAATCTGTCAGTTATTAAAATTTGTAAAATCTATTTATGAAAGGTC
2001 ATTAAACCAGATCATGTTCCTTTTTTTGTAATCAAGGTGACTAAGAAAATCAGTTGTGTAAATAAAATCATGTATCATAA
2081 AA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' ucGAUACGG-----UCGUAGAACGGa 5'
            | ||| |     ||| |||||:| 
Target 5' gaCCATGACTCATAAGCTTCTTGTCa 3'
1196 - 1221 139.00 -10.70
2
miRNA  3' ucgaUACGGUCGUAGAACgga 5'
              || | || ||||||   
Target 5' cagcATTCGAG-ATCTTGaga 3'
568 - 587 116.00 -9.42
3
miRNA  3' ucGAUACGGUCGUAGAACGga 5'
            ||   ||||  |||||:  
Target 5' caCTCCTCCAG--TCTTGTgg 3'
1257 - 1275 115.00 -9.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-31-5p :: RET    [ Functional MTI ]
Validation Method Luciferase reporter assay , qRT-PCR , Other
Conditions MDA-MB-231 , MCF-7
Disease breast cancer metastasis;
Location of target site 3'UTR
Tools used in this research PicTar , TargetScan
Original Description (Extracted from the article) ... Guided by this gene ontology analysis, we cloned the 3'UTRs of 16 putative miR-31 targets from these overrepresented categories, including several implicated in tumor invasion (Sahai and Marshall, 2002; McClatchey, 2003), into a luciferase construct.//{This MTI is not shown in Fig. 4} ...

- Valastyan, S. Reinhardt, F. Benaich, N. et al., 2009, Cell.

Article - Valastyan, S. Reinhardt, F. Benaich, N. et al.
- Cell, 2009
MicroRNAs are well suited to regulate tumor metastasis because of their capacity to coordinately repress numerous target genes, thereby potentially enabling their intervention at multiple steps of the invasion-metastasis cascade. We identify a microRNA exemplifying these attributes, miR-31, whose expression correlates inversely with metastasis in human breast cancer patients. Overexpression of miR-31 in otherwise-aggressive breast tumor cells suppresses metastasis. We deploy a stable microRNA sponge strategy to inhibit miR-31 in vivo; this allows otherwise-nonaggressive breast cancer cells to metastasize. These phenotypes do not involve confounding influences on primary tumor development and are specifically attributable to miR-31-mediated inhibition of several steps of metastasis, including local invasion, extravasation or initial survival at a distant site, and metastatic colonization. Such pleiotropy is achieved via coordinate repression of a cohort of metastasis-promoting genes, including RhoA. Indeed, RhoA re-expression partially reverses miR-31-imposed metastasis suppression. These findings indicate that miR-31 uses multiple mechanisms to oppose metastasis.
LinkOut: [PMID: 19524507]
MiRNA-Target Expression Profile:

 
MiRNA-Target Expression Profile(TCGA):

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
176 hsa-miR-31-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000088 RHOA ras homolog family member A 4 4
MIRT000490 PPP2R2A protein phosphatase 2, regulatory subunit B, alpha 3 1
MIRT000491 LATS2 LATS, large tumor suppressor, homolog 2 (Drosophila) 3 1
MIRT001180 FOXP3 forkhead box P3 4 2
MIRT004019 SELE selectin E 3 1
MIRT004597 CASR calcium-sensing receptor 1 1
MIRT004968 YY1 YY1 transcription factor 3 1
MIRT004969 RET ret proto-oncogene 3 1
MIRT004970 NUMB numb homolog (Drosophila) 3 1
MIRT004971 NFAT5 nuclear factor of activated T-cells 5, tonicity-responsive 3 1
MIRT004972 KLF13 Kruppel-like factor 13 3 1
MIRT004973 JAZF1 JAZF zinc finger 1 3 1
MIRT004974 HOXC13 homeobox C13 3 1
MIRT004975 ETS1 v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) 3 1
MIRT004976 ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) 4 1
MIRT004977 MPRIP myosin phosphatase Rho interacting protein 3 1
MIRT004978 MMP16 matrix metallopeptidase 16 (membrane-inserted) 4 1
MIRT004979 RDX radixin 4 2
MIRT004980 CXCL12 chemokine (C-X-C motif) ligand 12 3 2
MIRT004981 ARPC5 actin related protein 2/3 complex, subunit 5, 16kDa 3 1
MIRT004984 FZD3 frizzled family receptor 3 4 1
MIRT005456 DMD dystrophin 4 1
MIRT005566 TIAM1 T-cell lymphoma invasion and metastasis 1 4 1
MIRT005707 ICAM1 intercellular adhesion molecule 1 1 1
MIRT005874 DKK1 dickkopf 1 homolog (Xenopus laevis) 4 1
MIRT005875 DACT3 dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) 4 1
MIRT006026 WASF3 WAS protein family, member 3 2 1
MIRT006468 HIF1AN hypoxia inducible factor 1, alpha subunit inhibitor 3 2
MIRT006567 SATB2 SATB homeobox 2 4 5
MIRT007286 PRKCE protein kinase C, epsilon 1 1
MIRT007292 RASA1 RAS p21 protein activator (GTPase activating protein) 1 3 1
MIRT007319 STK40 serine/threonine kinase 40 2 2
MIRT007327 MCM2 minichromosome maintenance complex component 2 1 1
MIRT007328 CDK1 cyclin-dependent kinase 1 1 1
MIRT007367 CREG1 cellular repressor of E1A-stimulated genes 1 3 1
MIRT028387 Lats2 large tumor suppressor 2 1 1
MIRT035519 MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) 1 1
MIRT035528 MET met proto-oncogene (hepatocyte growth factor receptor) 2 3
MIRT049857 PPIL2 peptidylprolyl isomerase (cyclophilin)-like 2 1 1
MIRT049858 ZC3H18 zinc finger CCCH-type containing 18 1 1
MIRT049859 RPS7 ribosomal protein S7 1 1
MIRT049860 MED12 mediator complex subunit 12 1 1
MIRT049861 CYP27B1 cytochrome P450, family 27, subfamily B, polypeptide 1 1 1
MIRT049862 HIST1H2BC histone cluster 1, H2bc 1 1
MIRT049863 PTPRJ protein tyrosine phosphatase, receptor type, J 1 1
MIRT049864 AGO1 eukaryotic translation initiation factor 2C, 1 1 1
MIRT049865 HEATR2 HEAT repeat containing 2 1 1
MIRT049866 HIST1H2BK histone cluster 1, H2bk 1 1
MIRT049867 RPA1 replication protein A1, 70kDa 1 1
MIRT049868 TXNDC5 thioredoxin domain containing 5 (endoplasmic reticulum) 1 1
MIRT049869 AKAP8L A kinase (PRKA) anchor protein 8-like 1 1
MIRT049870 FLNA filamin A, alpha 1 1
MIRT049871 ATP2A2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 1 1
MIRT049872 NDFIP2 Nedd4 family interacting protein 2 1 1
MIRT049873 TRIB3 tribbles homolog 3 (Drosophila) 1 1
MIRT049874 NUP188 nucleoporin 188kDa 1 1
MIRT049875 RPS4Y1 ribosomal protein S4, Y-linked 1 1 1
MIRT049876 GLI2 GLI family zinc finger 2 1 1
MIRT049877 RPL37A ribosomal protein L37a 1 1
MIRT049878 EXOSC5 exosome component 5 1 1
MIRT049879 DDX19A DEAD (Asp-Glu-Ala-Asp) box polypeptide 19A 1 1
MIRT049880 UBA6 ubiquitin-like modifier activating enzyme 6 1 1
MIRT049881 NOL9 nucleolar protein 9 1 1
MIRT049882 DNAJC5 DnaJ (Hsp40) homolog, subfamily C, member 5 1 1
MIRT049883 FAM134C family with sequence similarity 134, member C 1 1
MIRT049884 PEX19 peroxisomal biogenesis factor 19 1 1
MIRT049885 SMEK2 SMEK homolog 2, suppressor of mek1 (Dictyostelium) 1 1
MIRT049886 TRRAP transformation/transcription domain-associated protein 1 1
MIRT049887 RANGAP1 Ran GTPase activating protein 1 1 1
MIRT049888 TNRC6B trinucleotide repeat containing 6B 1 1
MIRT049889 NOP56 NOP56 ribonucleoprotein homolog (yeast) 1 1
MIRT049890 PCSK1N proprotein convertase subtilisin/kexin type 1 inhibitor 1 1
MIRT049891 ZNF275 zinc finger protein 275 1 1
MIRT049892 C2CD5 KIAA0528 1 1
MIRT049893 NFATC2IP nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein 1 1
MIRT049894 HIST1H2BJ histone cluster 1, H2bj 1 1
MIRT049895 SRRM2 serine/arginine repetitive matrix 2 1 1
MIRT049896 GYG1 glycogenin 1 1 1
MIRT049897 ZC3H12C zinc finger CCCH-type containing 12C 1 1
MIRT049898 LRRC59 leucine rich repeat containing 59 1 1
MIRT049899 GHITM growth hormone inducible transmembrane protein 1 1
MIRT049900 EDC3 enhancer of mRNA decapping 3 homolog (S. cerevisiae) 1 1
MIRT049901 MYO1D myosin ID 1 1
MIRT049902 STOML2 stomatin (EPB72)-like 2 1 1
MIRT049903 EXOC6 exocyst complex component 6 1 1
MIRT049904 HOXA7 homeobox A7 1 1
MIRT049905 SFXN1 sideroflexin 1 1 1
MIRT049906 ILF2 interleukin enhancer binding factor 2, 45kDa 1 1
MIRT053750 SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) 2 1
MIRT053751 MAP4K4 mitogen-activated protein kinase kinase kinase kinase 4 2 1
MIRT053752 RAB27A RAB27A, member RAS oncogene family 2 1
MIRT054726 TBXA2R thromboxane A2 receptor 4 1
MIRT054727 C11orf30 chromosome 11 open reading frame 30 4 1
MIRT067320 SESN2 sestrin 2 1 2
MIRT248732 ARID1A AT rich interactive domain 1A (SWI-like) 3 1
MIRT267266 TMEM109 transmembrane protein 109 1 1
MIRT273599 SP1 Sp1 transcription factor 1 1
MIRT294476 ZNF805 zinc finger protein 805 1 1
MIRT303306 SNRNP27 small nuclear ribonucleoprotein 27kDa (U4/U6.U5) 1 1
MIRT316765 FOXC1 forkhead box C1 1 2
MIRT369398 YWHAE tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 1 4
MIRT395121 BAHD1 bromo adjacent homology domain containing 1 1 1
MIRT437432 ABCB9 ATP-binding cassette, sub-family B (MDR/TAP), member 9 3 1
MIRT437512 RHOBTB1 Rho-related BTB domain containing 1 1 1
MIRT437549 SP7 Sp7 transcription factor 3 1
MIRT443895 NUDT3 nudix (nucleoside diphosphate linked moiety X)-type motif 3 1 2
MIRT444977 C15orf52 chromosome 15 open reading frame 52 1 1
MIRT445239 FOXD4 forkhead box D4 1 1
MIRT445498 FOXD4L5 forkhead box D4-like 5 1 1
MIRT445501 FOXD4L4 forkhead box D4-like 4 1 1
MIRT447023 FOXD4L1 forkhead box D4-like 1 1 1
MIRT449247 MICA MHC class I polypeptide-related sequence A 1 1
MIRT450782 PAPOLG poly(A) polymerase gamma 1 1
MIRT453257 PARP11 poly (ADP-ribose) polymerase family, member 11 1 1
MIRT456182 ZDHHC6 zinc finger, DHHC-type containing 6 1 1
MIRT467509 SMG1 smg-1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans) 1 1
MIRT468710 SDC4 syndecan 4 1 1
MIRT469164 RNF111 ring finger protein 111 1 1
MIRT471166 PHF12 PHD finger protein 12 1 1
MIRT472350 TSPAN1 tetraspanin 1 1 1
MIRT477957 DPM2 dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit 1 1
MIRT481316 ATP5A1 ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle 1 1
MIRT481631 ARF1 ADP-ribosylation factor 1 1 1
MIRT484657 HOXD3 homeobox D3 1 2
MIRT485314 MZT1 mitotic spindle organizing protein 1 1 2
MIRT488734 MXRA7 matrix-remodelling associated 7 1 1
MIRT488972 REXO2 REX2, RNA exonuclease 2 homolog (S. cerevisiae) 1 1
MIRT494953 IL5RA interleukin 5 receptor, alpha 1 1
MIRT496171 RPL35A ribosomal protein L35a 1 1
MIRT498328 NF2 neurofibromin 2 (merlin) 1 1
MIRT499576 INTU inturned planar cell polarity effector homolog (Drosophila) 1 1
MIRT502931 CDC42SE1 CDC42 small effector 1 1 2
MIRT506784 KLHL15 kelch-like 15 (Drosophila) 1 3
MIRT508501 RSRC1 arginine/serine-rich coiled-coil 1 1 3
MIRT509453 ZNF587 zinc finger protein 587 1 3
MIRT509855 ZNF641 zinc finger protein 641 1 2
MIRT516241 BCAS4 breast carcinoma amplified sequence 4 1 1
MIRT516565 PARP1 poly (ADP-ribose) polymerase 1 1 2
MIRT525051 FRK fyn-related kinase 1 1
MIRT534347 SFT2D2 SFT2 domain containing 2 1 1
MIRT539168 AR androgen receptor 1 1
MIRT539748 CNBP CCHC-type zinc finger, nucleic acid binding protein 1 1
MIRT540716 GUF1 GUF1 GTPase homolog (S. cerevisiae) 1 1
MIRT540987 ZNF460 zinc finger protein 460 1 2
MIRT541504 TOR1AIP1 torsin A interacting protein 1 1 1
MIRT542421 ZNF331 zinc finger protein 331 1 1
MIRT549566 C19orf12 chromosome 19 open reading frame 12 1 1
MIRT549839 ECHDC1 enoyl CoA hydratase domain containing 1 1 1
MIRT551148 ZNF678 zinc finger protein 678 1 1
MIRT551646 CCDC127 coiled-coil domain containing 127 1 1
MIRT552507 ZIK1 zinc finger protein interacting with K protein 1 homolog (mouse) 1 2
MIRT553858 SYDE2 synapse defective 1, Rho GTPase, homolog 2 (C. elegans) 1 1
MIRT555063 PYURF PIGY upstream reading frame 1 1
MIRT556625 LAPTM4A lysosomal protein transmembrane 4 alpha 1 1
MIRT564127 FZD1 frizzled family receptor 1 1 1
MIRT564146 GTF2E1 general transcription factor IIE, polypeptide 1, alpha 56kDa 1 1
MIRT564314 CCNT1 cyclin T1 1 1
MIRT565481 SPRTN SprT-like N-terminal domain 1 1
MIRT570106 SLC18B1 solute carrier family 18, subfamily B, member 1 1 1
MIRT571745 QSER1 glutamine and serine rich 1 1 1
MIRT572342 CKAP2L cytoskeleton associated protein 2-like 1 2
MIRT622081 SRPX2 sushi-repeat containing protein, X-linked 2 1 1
MIRT637924 LILRA2 leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2 1 1
MIRT641554 LIPG lipase, endothelial 1 1
MIRT644331 IPP intracisternal A particle-promoted polypeptide 1 1
MIRT654835 PPM1L protein phosphatase, Mg2+/Mn2+ dependent, 1L 1 1
MIRT664036 RPL27A ribosomal protein L27a 1 1
MIRT682946 RPL12 ribosomal protein L12 1 1
MIRT689545 XPO6 exportin 6 1 1
MIRT693637 ACBD7 acyl-CoA binding domain containing 7 1 1
MIRT702575 JARID2 jumonji, AT rich interactive domain 2 1 1
MIRT705803 AKNA AT-hook transcription factor 1 1
MIRT706577 AFF1 AF4/FMR2 family, member 1 1 1
MIRT719138 DPYSL5 dihydropyrimidinase-like 5 1 1
MIRT720757 FAM193A family with sequence similarity 193, member A 1 1
MIRT722723 MCMBP minichromosome maintenance complex binding protein 1 1
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