Accession ID: MIRT005035 [miRNA, hsa-miR-200b :: MATR3, target gene]
|Description||Homo sapiens miR-200b stem-loop|
|Comment||The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .|
|2nd Structure of pre-miRNA|
|Mature Sequence||57| UAAUACUGCCUGGUAAUGAUGA |78|
|Putative hsa-miR-200b-3p Targets|
|Mature Sequence||21| CAUCUUACUGGGCAGCAUUGGA |42|
|Putative hsa-miR-200b-5p Targets|
|Synonyms||DKFZp686K0542, DKFZp686K23100, KIAA0723, MGC9105, MPD2|
|Putative miRNA Targets on MATR3|
|3'UTR of MATR3
(miRNA target sites are highlighted)
>MATR3|NM_018834|3'UTR 1 TAAGATGTGCAAGGAGATTTAATGATTTCAAAGAAAATAATGGTTCTTTGTTTTTAATGTTAACCTTTTTTAAATACAAT 81 ACTGATAGTTAGAAGAAAACTATTGTACTCTTTTGTTTTAGTGGAGAAATAATAGATGTCTGTTCATGTGTTAAGTGTTA 161 TAGCAAAAAAAATACACATATGGTTAAGTTAATGAATAGTTTTTGTTTTATCAGAATGGCAACAGACAGAAGTACTTTGT 241 AGAGATTGACTTCCTAAGCTACTTAAGACAACTTGCACCACTAAGAAAAAAATGTAGAACCATTTGGAAAAATGAAATTT 321 AGTAGTTCCAAGTTTCAAAGAAATGTCAACATTTTATTCCATTCAATAAAGAACAAAACCAATAGTGTTTTTATTACTTT 401 CATCTGAAACATTCCATGTTTTAATCTGAGCCTTGCAGACTTTCATTTGGAGTTTGAACCCGTTTTGGTTGCATTTCATT 481 TTTGGAGAACTTAATTAACGTGAGATTGGCAATTGAAATGCAGGTGCAGTTTTCTGTTAATGTCATGCTGTTGTTTAGGT 561 AATAAGAAATATTAAGTAATTGGCTTTAGATTTTGTAATTTTTTTCCCTGAGTTCCTGCTAGATTTCGTATTCTAGTAGT 641 CAATGTATTTTCAGTGAAATGCAAAAATATTCCCGTTATCTTTGACCAGTATTAATTTTTGAGATCTTACTGCTTGTCAC 721 TTGAATCCCGTGATTGTCATACATCTCTGGTATAAGCAACATTTGATTTTTGAAGTGTGTAGACCATCTCTTCATATTTT 801 CAAGATGTAATTTTACATTTCTGCATTTTTAAAACAGTTTGGCCATAATCCTAGATGCACGCTTCTAATTCATGTACCTG 881 CACATGTGACCTTTGTGAACAGAAATTTGCATGTATAATTTGTGTTTACTTGTAACTTTCTGGTTATATACTGCTTATAT 961 CTGTGGATTCAAGTTACTGAAGTGAATACCAATAAAAAGAAAACCCTAGGCCATGTTAATTGGTTATACATGTTTGGAAT 1041 GTTAACCAAAAAAAAAAAAAAAA
|miRNA-target interactions (Predicted by miRanda)||
|miRNA:Target||hsa-miR-200b :: MATR3 [ Functional MTI ]|
|Validation Method||Luciferase reporter assay , Microarray , qRT-PCR|
|Location of target site||3'UTR|
|Tools used in this research||TargetScan|
|Original Description (Extracted from the article)||... Interestingly, we detected miR-200b targeting directly Matrin 3 (MATR3) expression in an adrenocortical cancer cell line (H295R).// ...
- Bimpaki, E. I. Iliopoulos, D. Moraitis, A. et al., 2010, Clin Endocrinol (Oxf).
|miRNA-target interactions (Provided by authors)||
MicroRNA signature in massive macronodular adrenocortical disease and implications for adrenocortical tumourigenesis- Bimpaki, E. I. Iliopoulos, D. Moraitis, A. et al.
- Clin Endocrinol (Oxf), 2010
PURPOSE: Massive macronodular adrenocortical disease (MMAD) may be caused by aberrant microRNA expression. To determine the microRNA profile in MMAD and identify putative microRNA-gene target pairs involved in adrenal tumourigenesis. EXPERIMENTAL DESIGN: We performed microRNA microarray analysis in 10 patients with ACTH-independent Cushing syndrome caused by MMAD (ages 39-60 years) and four normal adrenal cortex samples were used as controls. Microarray data were validated by real-time polymerase chain reaction (qRT-PCR). Identification of potential microRNA-gene target pairs implicated in MMAD pathogenesis has been performed by integrating our microRNA data with previously obtained cDNA microarray data. Experimental validation of specific microRNA gene targets was performed by transfection experiments and luciferase assay. RESULTS: A total of 37 microRNAs were differentially expressed between MMAD and normal tissues; 16 microRNAs were down-regulated, including miR-200b and miR-203, whereas 21 microRNAs were up-regulated, miR-210 and miR-484 among them. Comparison of microRNA data with different clinicopathological parameters revealed miR-130a and miR-382 as putative diagnostic MMAD markers. Interestingly, we detected miR-200b targeting directly Matrin 3 (MATR3) expression in an adrenocortical cancer cell line (H295R). CONCLUSIONS: MicroRNAs appear to have distinct regulatory effects in MMAD, including an association with clinical presentation and severity of the disease, expressed by the degree of hypercortisolism. This is the first investigation of microRNAs in MMAD, a disease with complex pathogenesis; the data indicate that specific microRNAs such as miR-200b may play a significant role in MMAD formation and/or progression.LinkOut: [PMID: 19849700]