Accession ID: MIRT005035 [miRNA, hsa-miR-200b :: MATR3, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-200b LinkOut: [miRBase ]
Description Homo sapiens miR-200b stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-200b-3p
Mature Sequence 57| UAAUACUGCCUGGUAAUGAUGA |78
Evidence Experimental
Experiments Northern
Putative hsa-miR-200b-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-200b-5p
Mature Sequence 21| CAUCUUACUGGGCAGCAUUGGA |42
Evidence Experimental
Experiments Cloned
Putative hsa-miR-200b-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol MATR3 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms DKFZp686K0542, DKFZp686K23100, KIAA0723, MGC9105, MPD2
Description matrin 3
Transcript NM_018834   LinkOut: [ RefSeq ]
Other Transcripts NM_199189   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on MATR3 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of MATR3
(miRNA target sites are highlighted)		 
>MATR3|NM_018834|3'UTR
   1 TAAGATGTGCAAGGAGATTTAATGATTTCAAAGAAAATAATGGTTCTTTGTTTTTAATGTTAACCTTTTTTAAATACAAT
  81 ACTGATAGTTAGAAGAAAACTATTGTACTCTTTTGTTTTAGTGGAGAAATAATAGATGTCTGTTCATGTGTTAAGTGTTA
 161 TAGCAAAAAAAATACACATATGGTTAAGTTAATGAATAGTTTTTGTTTTATCAGAATGGCAACAGACAGAAGTACTTTGT
 241 AGAGATTGACTTCCTAAGCTACTTAAGACAACTTGCACCACTAAGAAAAAAATGTAGAACCATTTGGAAAAATGAAATTT
 321 AGTAGTTCCAAGTTTCAAAGAAATGTCAACATTTTATTCCATTCAATAAAGAACAAAACCAATAGTGTTTTTATTACTTT
 401 CATCTGAAACATTCCATGTTTTAATCTGAGCCTTGCAGACTTTCATTTGGAGTTTGAACCCGTTTTGGTTGCATTTCATT
 481 TTTGGAGAACTTAATTAACGTGAGATTGGCAATTGAAATGCAGGTGCAGTTTTCTGTTAATGTCATGCTGTTGTTTAGGT
 561 AATAAGAAATATTAAGTAATTGGCTTTAGATTTTGTAATTTTTTTCCCTGAGTTCCTGCTAGATTTCGTATTCTAGTAGT
 641 CAATGTATTTTCAGTGAAATGCAAAAATATTCCCGTTATCTTTGACCAGTATTAATTTTTGAGATCTTACTGCTTGTCAC
 721 TTGAATCCCGTGATTGTCATACATCTCTGGTATAAGCAACATTTGATTTTTGAAGTGTGTAGACCATCTCTTCATATTTT
 801 CAAGATGTAATTTTACATTTCTGCATTTTTAAAACAGTTTGGCCATAATCCTAGATGCACGCTTCTAATTCATGTACCTG
 881 CACATGTGACCTTTGTGAACAGAAATTTGCATGTATAATTTGTGTTTACTTGTAACTTTCTGGTTATATACTGCTTATAT
 961 CTGTGGATTCAAGTTACTGAAGTGAATACCAATAAAAAGAAAACCCTAGGCCATGTTAATTGGTTATACATGTTTGGAAT
1041 GTTAACCAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' agUAGUAAUGGUCCGUCAUAAu 5'
            ||| ||: |   ||||||| 
Target 5' ttATCTTTGAC---CAGTATTa 3'
 		676 - 694 153.00 -8.70
2 
miRNA  3' agUAGUAAUGGUCCGUCAUAAu 5'
            | ||  ||||  :|||:|| 
Target 5' agAACAAAACCA-ATAGTGTTt 3'
 		370 - 390 123.00 -9.60
3 
miRNA  3' agUAG--UAAUGGUC-CGUCAUAAu 5'
            ||:  | |:| :| ||||| || 
Target 5' caATTGAAATGCAGGTGCAGTTTTc 3'
 		510 - 534 122.00 -7.66
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-200b :: MATR3    [ Functional MTI ]
Validation Method Luciferase reporter assay , Microarray , qRT-PCR
Conditions H295R
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... Interestingly, we detected miR-200b targeting directly Matrin 3 (MATR3) expression in an adrenocortical cancer cell line (H295R).// ...

- Bimpaki, E. I. Iliopoulos, D. Moraitis, A. et al., 2010, Clin Endocrinol (Oxf).
miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1 
miRNA  3' agUAGUAAUGGUCCGUCAUAAu 5'
            ||| ||: |   ||||||| 
Target 5' uuAUCUUUGAC---CAGUAUUa 3'
 		5 - 23
Article - Bimpaki, E. I. Iliopoulos, D. Moraitis, A. et al.
- Clin Endocrinol (Oxf), 2010
PURPOSE: Massive macronodular adrenocortical disease (MMAD) may be caused by aberrant microRNA expression. To determine the microRNA profile in MMAD and identify putative microRNA-gene target pairs involved in adrenal tumourigenesis. EXPERIMENTAL DESIGN: We performed microRNA microarray analysis in 10 patients with ACTH-independent Cushing syndrome caused by MMAD (ages 39-60 years) and four normal adrenal cortex samples were used as controls. Microarray data were validated by real-time polymerase chain reaction (qRT-PCR). Identification of potential microRNA-gene target pairs implicated in MMAD pathogenesis has been performed by integrating our microRNA data with previously obtained cDNA microarray data. Experimental validation of specific microRNA gene targets was performed by transfection experiments and luciferase assay. RESULTS: A total of 37 microRNAs were differentially expressed between MMAD and normal tissues; 16 microRNAs were down-regulated, including miR-200b and miR-203, whereas 21 microRNAs were up-regulated, miR-210 and miR-484 among them. Comparison of microRNA data with different clinicopathological parameters revealed miR-130a and miR-382 as putative diagnostic MMAD markers. Interestingly, we detected miR-200b targeting directly Matrin 3 (MATR3) expression in an adrenocortical cancer cell line (H295R). CONCLUSIONS: MicroRNAs appear to have distinct regulatory effects in MMAD, including an association with clinical presentation and severity of the disease, expressed by the degree of hypercortisolism. This is the first investigation of microRNAs in MMAD, a disease with complex pathogenesis; the data indicate that specific microRNAs such as miR-200b may play a significant role in MMAD formation and/or progression.
LinkOut: [PMID: 19849700]