Accession ID: MIRT005181 [miRNA, hsa-miR-30a :: GNAI2, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-30a LinkOut: [miRBase ]
Description Homo sapiens miR-30a stem-loop
Comment The mature sequences miR-30 . Related miRNAs are processed from the 5' arms of other precursor loci (mir-30b, MIR:MI0000441; mir-30c-1, MIR:MI0000736; mir-30c-2, MIR:MI0000254; mir-30d, MIR:MI0000255; mir-30e, MI0000749).
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-30a-3p
Mature Sequence 47| CUUUCAGUCGGAUGUUUGCAGC |68
Evidence Experimental
Experiments Cloned
Expression Profile
Putative hsa-miR-30a-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-30a-5p
Mature Sequence 6| UGUAAACAUCCUCGACUGGAAG |27
Evidence Experimental
Experiments Cloned
Expression Profile
Putative hsa-miR-30a-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol GNAI2 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
Description guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Transcript NM_001166425   LinkOut: [ RefSeq ]
Other Transcripts NM_002070   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04062    Chemokine signaling pathway - Homo sapiens (human)
hsa04360    Axon guidance - Homo sapiens (human)
hsa04530    Tight junction - Homo sapiens (human)
hsa04540    Gap junction - Homo sapiens (human)
hsa04670    Leukocyte transendothelial migration - Homo sapiens (human)
hsa04730    Long-term depression - Homo sapiens (human)
hsa04914    Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa04916    Melanogenesis - Homo sapiens (human)
hsa05142    Chagas disease - Homo sapiens (human)
Putative miRNA Targets on GNAI2 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of GNAI2
(miRNA target sites are highlighted)		 
>GNAI2|NM_001166425|3'UTR
   1 TGAGGGGCAGCGGGGCCTGGCGGGATGGGCCACCGCCGACTTTGTACCCCCCAACCCCTGAGGAAGATGGGGGCAAGAAG
  81 ATCACGCTCCCCGCCTGTTCCCCCGCCGCTTTTCTCCTCTTTCCTCTCTTTGTTCTCAGCTCCCCCTGTCCCCTCAGCTC
 161 CAGACGTAGGGGAGGGGTTGCCACAGGCCTCCCTGTTTGAAGCCTGCCCTTGTCTGAGATGCTGGTAATGGCCATGGTAC
 241 CCCCTTCTGGGCATCTGTTCTGGTTTTTAACCATTGTCTTGTTCTGTGATGAGGGGAGGGGGGCACATGCTGAGTCTCCC
 321 AAGGCTGCGTCTGGAGGGGCCCCTGCTTCTCCAGCCTGGACCCCCAGCTTTGCCCAACACCAGCCCCTGCCCCAGCCCAA
 401 GTCCAAATGTTTACAGGGAGCCTCCTGCCCAGTCCCCCAACCCCAGCCGCTCGGAGGCCCCAAAGGAAAAAGCACAAGAA
 481 GCGTGAGACGCCACCATTCCTGGAAACCACAGTCCACCTGCTCATTCTCGTAGCTTTTTAAAAAAATGAAAGTAAAGGAA
 561 AAAAAAAAAACTGCAAATCTAGAAAACTTTTTAGAGAAAAACTATTTAAAACTGTCAGATCCTGACCAGCAAGCCCCCCC
 641 CCAGCCCCCCTTCCAAGTGACTCCGTGCCTTGAGTGTGTCTGCGTGTTTACACCCGTCCCTCTGCTGGCCGCCCCCGTGC
 721 GAGCGGCACCCCTGCCCTGCCCTCCACAGAATTGGGTTCCAAGGGCTGTTCCAGACAACTGCCAACGTCACTGAGGGCCC
 801 TGCCCCAGCGGCCCTGGCCCCAGGCTCTATTAACCTAAAATGTAGCTCCCTAGCGCTAACCTAGGAACCGCCGCTGCCTG
 881 CTGGGGGGCCACGCCCCTCATGCCCTTGTCCCAGGCCCGGGGCCTTCAGCGTTGAACACTTCCTTGCTTTTTTCACATGT
 961 TTTATGGAATTGTTCACCTGGTTTGAAATAATAAAATGTAGAAAGAAAAAAAAT
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' cgACGUUUGUAGGCUGACUUUc 5'
            ||| |||:||  |||||:: 
Target 5' acTGCCAACGTC--ACTGAGGg 3'
 		778 - 797 136.00 -21.20
2 
miRNA  3' cgaCGUUUGUAGGCUGACUUUc 5'
             || | |||:|  |||:|| 
Target 5' gacGCCACCATTC--CTGGAAa 3'
 		487 - 506 118.00 -7.40
3 
miRNA  3' cgacgUUUGUAGGCUGACUUUc 5'
               ||| |    |||| || 
Target 5' aggaaAAAAAAAAAACTGCAAa 3'
 		556 - 577 113.00 -6.40
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-30a :: GNAI2    [ Functional MTI ]
Validation Method pSILAC
Conditions HeLa
Original Description (Extracted from the article) ... Provided by pSILAC database (http://psilac.mdc-berlin.de/) ...

- Selbach, M. Schwanhausser, B. Thierfelder, et al., 2008, Nature.
Article - Selbach, M. Schwanhausser, B. Thierfelder, et al.
- Nature, 2008
Animal microRNAs (miRNAs) regulate gene expression by inhibiting translation and/or by inducing degradation of target messenger RNAs. It is unknown how much translational control is exerted by miRNAs on a genome-wide scale. We used a new proteomic approach to measure changes in synthesis of several thousand proteins in response to miRNA transfection or endogenous miRNA knockdown. In parallel, we quantified mRNA levels using microarrays. Here we show that a single miRNA can repress the production of hundreds of proteins, but that this repression is typically relatively mild. A number of known features of the miRNA-binding site such as the seed sequence also govern repression of human protein synthesis, and we report additional target sequence characteristics. We demonstrate that, in addition to downregulating mRNA levels, miRNAs also directly repress translation of hundreds of genes. Finally, our data suggest that a miRNA can, by direct or indirect effects, tune protein synthesis from thousands of genes.
LinkOut: [PMID: 18668040]