Accession ID: MIRT005349 [miRNA, hsa-miR-155 :: FLI1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-155 LinkOut: [miRBase ]
Description Homo sapiens miR-155 stem-loop
Comment Human mir-155 is predicted based on homology to a cloned miR from mouse (MIR:MI0000177) .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-155-3p
Mature Sequence 43| CUCCUACAUAUUAGCAUUAACA |64
Evidence Experimental
Experiments Cloned
Putative hsa-miR-155-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-155-5p
Mature Sequence 4| UUAAUGCUAAUCGUGAUAGGGGU |26
Evidence Experimental
Experiments Cloned
Putative hsa-miR-155-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol FLI1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms EWSR2, SIC-1
Description Friend leukemia virus integration 1
Transcript NM_001167681   LinkOut: [ RefSeq ]
Other Transcripts NM_002017   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on FLI1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of FLI1
(miRNA target sites are highlighted)		 
>FLI1|NM_001167681|3'UTR
   1 TAGAAGCTTACTCATCAGTGGCCTTCTAGCTGAAGCCCATCCTGCACACTTACTGGATGCTTTGGACTCAACAGGACATA
  81 TGTGGCCTTGAAGGGAAGACAAAACTGGATGTTCTTTCTTGTTGGATAGAACCTTTGTATTTGTTCTTTAAAAACATTTT
 161 TTTTAATGTTGGTAACTTTTGCTTCCTCTACCTGAACAAAGAGATGAATAATTCCATGGGCCAGTATGCCAGTTTGAATT
 241 CTCAGTCTCCTAGCATCTTGTGAGTTGCATATTAAGATTACTGGAATGGTTAAGTCATGGTTCTGAGAAAGAAGCTGTAC
 321 GTTTTCTTTATGTTTTTATGACCAAAGCAGTTTCTTGTCAATACACGGGGTTCAGTATGACACAGAATCATGGACTTAAC
 401 CCGTCATGTTCTGGTTTGAGATTTAGTGACAAATAGAGGTGGGAAGCTTATAATCTAATTTTAGGAGGACCAAATTCAGT
 481 GGATGGCAACTGGAACATTGATTGTAAGGCCAGTGAAGTTTTCACCCAACTGGAATTTGATGGAAAGAAGGTTTGTGTGT
 561 TTAAGACGCCAAGGGCATTGCAGAATCCCTCTCAGTGGACAGTATGCACTCAGCTGACCACTCTCTCTAGAAATAGTCAA
 641 GATATGAACTAAGAAATTTTAATGCAAATACATACATTCCTGAAAGACGGGGAATTAAATTACTAATTTTTTTTTTTTTT
 721 TAAATGATGACAGTGGTCCCAGAACTTGGAAAAGTTGTAGGGATTTCTAAACTCAAGCAGATTCGCAAGTGCTGTGCGCT
 801 TGTCAGACCATCAGACCAGGGCCAACCAATCAGAAGGCAACTTACTGTATAAATTATGCAGAGTTATTTTCCTATATCTC
 881 ACAGTATTAAAAATAAATAATTAAAAATTAAGAATAAATAAACGAGTTGACCTCGGTCACAAAAGCAGTTTTACTATCGA
 961 ATCAATCGCTGTTATTTTTTTTAATGTAATTTGTACATCTTTTTTCAATCTGTACATTTGGGCTGTCTGTATGTTTTTAT
1041 AGCTGGTTTTTAAAAAGCATAATATGCCTATAGCTGAAAAGGAAACAGGGCTGTTTAAGTCACTGACTTATGAGAAAGCA
1121 AAGCACTGGTACAGTTATTTAACAGGCATACACAAGCAGGGAAAAGATAATCCATTTAGATCTTTAATGCTTTGGAAATG
1201 CGTGTAACAGTACTGCAATAATCACAGCTCTGGGAAAAACAACGAAACTTTCCCTTGTGGAGAGGAGGGATTTTCCTGCT
1281 CTATATAAGCAACATATTTTTAGACATTAAAATATATATAATTTTGCAGGTAATTGTTGACTTTTTTAACTATATTAAGT
1361 GTTAAGCTGACAACTGTCAAAGAAGACCATGTTGTAAAATAATTTGACTAAATAAATGGTTCCTTCTCTCAGTGCTGAGG
1441 ACAGTTTTCTTATTTACCGCCCCCGTTAGGTCAAAGGGTTTTCCCTGGGGAACTTTCCTATTTACTTCTTGCACTATCAA
1521 GAATTTTTCGAATGTACCTACTGCAGTACAGCAGAAGGTAAAAAATCAGTGTGGTTTTTCATTGTTGTTGATGATGTTTG
1601 TAGTGTTTTTGTGTGTGTTATTTAAATCTTCCTCCAGCCTAAAAGGGTTTTATAAAACAGCAGCTAAGGCCATGGATAAA
1681 CCTGTATGTAAGGACTGGAGCAAAGCGAGCTGGTCTATCCAGACTGGTCTGTGAGATTTAACTCTGCAGCCTCCCCTGGG
1761 CACTTCAGACCCAGACGGCCACCTTCTGCCACTCCAGCAAAGAATAAGCGCCCTGCTTCCTTCAGGTCTCAGACCAGGAC
1841 TTTATGGCTCATGCAGATTTTTAAGGTCATTTTTCTTCCCAAGGAAGAAACTTGCCTCCAGTTCCTTCACTGTTAGGTAG
1921 CTTATTTTCATTTTCTCTATTTTACAATGAAAAGAGTGAGACCTGGGAAGTCCTTGATTTGCAAGGAATTAGACTCACAG
2001 CATTGGTAACCCTAGAACCTTCTTAGGGTAACACTAAGTACCTTCTAGACAACATGTCTACCTAAATGAAATGGGATGTG
2081 TTTCGGAACATTTGTCTCCAGTTTTTTTTTAATCTTGCACCCTGCCATTTAAAAAGATGTGTAAAGCACATATTCTCAAC
2161 ATATGCACATTGATTTATAAATCATATATACAAACTGTTACATTATTCTTCATATTAGAAAACAAATACAAAATAGAACA
2241 TTTTAAATGGTGATATAAAAATAAATTGAAACTGAAATTCTAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' acaAUUACGAUUAUACAUCCUc 5'
             |||| ||||| | ||||| 
Target 5' ttaTAAT-CTAAT-TTTAGGAg 3'
 		448 - 467 137.00 -11.80
2 
miRNA  3' acaAUUACGAUUAUACA-UCCUc 5'
             |||  || :||||| |||| 
Target 5' ggaTAAACCT-GTATGTAAGGAc 3'
 		1674 - 1695 130.00 -7.80
3 
miRNA  3' acaauuacgaUUAUACAUCCUc 5'
                    ||  ||||||: 
Target 5' gaacttggaaAAGTTGTAGGGa 3'
 		742 - 763 128.00 -7.40
Experimental Support 1 for Non-Functional miRNA-Target Interaction
miRNA:Target hsa-miR-155 :: FLI1    [ Non-Functional MTI ]
Validation Method Western blot
Conditions K562
Location of target site 3'UTR
Original Description (Extracted from the article) ... Importantly FLI1, another relevant MK gene (Spyropoulos et al, 2000; Raslova et al, 2004), not predicted to be a MIRN155 target, was not affected (Fig 5C). ...

- Romania, P. Lulli, V. Pelosi, E. Biffoni, et al., 2008, Br J Haematol.
Article - Romania, P. Lulli, V. Pelosi, E. Biffoni, et al.
- Br J Haematol, 2008
MicroRNAs (miRNAs) control basic biological functions and are emerging as key regulators of haematopoiesis. This study focused on the functional role of MIRN155 on megakaryocytic (MK) differentiation of human cord blood CD34+ haematopoietic progenitor cells (HPCs). MIRN155, abundantly expressed in early HPCs, decreases sharply during MK differentiation. Functional studies showed that enforced expression of MIRN155 impairs proliferation and differentiation of MK cells. Furthermore, HPCs transfected with MIRN155 showed a significant reduction of their MK clonogenic capacity, suggesting that down-modulation of this miRNA favours MK progenitor differentiation. Consistent with this observation, MIRN155 downregulates, by directly binding to their 3'-UTR, the expression of Ets-1 and Meis1, two transcription factors with well-known functions in MK cells. These results show that the decline of MIRN155 is required for MK proliferation and differentiation at progenitors and precursors level and indicate that sustained expression of MIRN155 inhibits megakaryopoiesis.
LinkOut: [PMID: 18950466]