Accession ID: MIRT005418 [miRNA, hsa-miR-30d :: TP53, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-30d LinkOut: [miRBase ]
Description Homo sapiens miR-30d stem-loop
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-30d-3p
Mature Sequence 46| CUUUCAGUCAGAUGUUUGCUGC |67
Evidence Experimental
Experiments Cloned
Putative hsa-miR-30d-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-30d-5p
Mature Sequence 6| UGUAAACAUCCCCGACUGGAAG |27
Evidence Experimental
Experiments Cloned
Putative hsa-miR-30d-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol TP53 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms FLJ92943, LFS1, TRP53, p53
Description tumor protein p53
Transcript NM_001126114   LinkOut: [ RefSeq ]
Other Transcripts NM_001126112, NM_000546, NM_001126113, NM_001126115, NM_001126116, NM_001126117   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04010    MAPK signaling pathway - Homo sapiens (human)
hsa04110    Cell cycle - Homo sapiens (human)
hsa04115    p53 signaling pathway - Homo sapiens (human)
hsa04210    Apoptosis - Homo sapiens (human)
hsa04310    Wnt signaling pathway - Homo sapiens (human)
hsa04722    Neurotrophin signaling pathway - Homo sapiens (human)
hsa05014    Amyotrophic lateral sclerosis (ALS) - Homo sapiens (human)
hsa05016    Huntington's disease - Homo sapiens (human)
hsa05200    Pathways in cancer - Homo sapiens (human)
hsa05210    Colorectal cancer - Homo sapiens (human)
hsa05212    Pancreatic cancer - Homo sapiens (human)
hsa05213    Endometrial cancer - Homo sapiens (human)
hsa05214    Glioma - Homo sapiens (human)
hsa05215    Prostate cancer - Homo sapiens (human)
hsa05216    Thyroid cancer - Homo sapiens (human)
hsa05217    Basal cell carcinoma - Homo sapiens (human)
hsa05218    Melanoma - Homo sapiens (human)
hsa05219    Bladder cancer - Homo sapiens (human)
hsa05220    Chronic myeloid leukemia - Homo sapiens (human)
hsa05222    Small cell lung cancer - Homo sapiens (human)
hsa05223    Non-small cell lung cancer - Homo sapiens (human)
Putative miRNA Targets on TP53 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of TP53
(miRNA target sites are highlighted)		 
>TP53|NM_001126114|3'UTR
   1 TAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATACAGATGCTACTTGACTTACGATGGTGTTACTTCCTGATAA
  81 ACTCGTCGTAAGTTGAAAATATTATCCGTGGGCGTGAGCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTC
 161 AAGGATGCCCAGGCTGGGAAGGAGCCAGGGGGGAGCAGGGCTCACTCCAGCCACCTGAAGTCCAAAAAGGGTCAGTCTAC
 241 CTCCCGCCATAAAAAACTCATGTTCAAGACAGAAGGGCCTGACTCAGACTGACATTCTCCACTTCTTGTTCCCCACTGAC
 321 AGCCTCCCACCCCCATCTCTCCCTCCCCTGCCATTTTGGGTTTTGGGTCTTTGAACCCTTGCTTGCAATAGGTGTGCGTC
 401 AGAAGCACCCAGGACTTCCATTTGCTTTGTCCCGGGGCTCCACTGAACAAGTTGGCCTGCACTGGTGTTTTGTTGTGGGG
 481 AGGAGGATGGGGAGTAGGACATACCAGCTTAGATTTTAAGGTTTTTACTGTGAGGGATGTTTGGGAGATGTAAGAAATGT
 561 TCTTGCAGTTAAGGGTTAGTTTACAATCAGCCACATTCTAGGTAGGGGCCCACTTCACCGTACTAACCAGGGAAGCTGTC
 641 CCTCACTGTTGAATTTTCTCTAACTTCAAGGCCCATATCTGTGAAATGCTGGCATTTGCACCTACCTCACAGAGTGCATT
 721 GTGAGGGTTAATGAAATAATGTACATCTGGCCTTGAAACCACCTTTTATTACATGGGGTCTAGAACTTGACCCCCTTGAG
 801 GGTGCTTGTTCCCTCTCCCTGTTGGTCGGTGGGTTGGTAGTTTCTACAGTTGGGCAGCTGGTTAGGTAGAGGGAGTTGTC
 881 AAGTCTCTGCTGGCCCAGCCAAACCCTGTCTGACAACCTCTTGGTGAACCTTAGTACCTAAAAGGAAATCTCACCCCATC
 961 CCACACCCTGGAGGATTTCATCTCTTGTATATGATGATCTGGATCCACCAAGACTTGTTTTATGCTCAGGGTCAATTTCT
1041 TTTTTCTTTTTTTTTTTTTTTTTTCTTTTTCTTTGAGACTGGGTCTCGCTTTGTTGCCCAGGCTGGAGTGGAGTGGCGTG
1121 ATCTTGGCTTACTGCAGCCTTTGCCTCCCCGGCTCGAGCAGTCCTGCCTCAGCCTCCGGAGTAGCTGGGACCACAGGTTC
1201 ATGCCACCATGGCCAGCCAACTTTTGCATGTTTTGTAGAGATGGGGTCTCACAGTGTTGCCCAGGCTGGTCTCAAACTCC
1281 TGGGCTCAGGCGATCCACCTGTCTCAGCCTCCCAGAGTGCTGGGATTACAATTGTGAGCCACCACGTCCAGCTGGAAGGG
1361 TCAACATCTTTTACATTCTGCAAGCACATCTGCATTTTCACCCCACCCTTCCCCTCCTTCTCCCTTTTTATATCCCATTT
1441 TTATATCGATCTCTTATTTTACAATAAAACTTTGCTGCCACCTGTGTGTCTGAGGGGTG
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' cgUCGUU--UGUAGACUGACUUUc 5'
            ||| |  ||:||  :|||:|| 
Target 5' tgAGCCACCACGTCCAGCTGGAAg 3'
 		1335 - 1358 130.00 -15.30
2 
miRNA  3' cgucGUUUGUAGACUGACUUUc 5'
              | :|| || |||||| | 
Target 5' gggcCTGAC-TCAGACTGACAt 3'
 		275 - 295 125.00 -8.72
3 
miRNA  3' cgucGUUUGUAGACUGACUUuc 5'
              |:::  ||  ||||||  
Target 5' gtccCGGGGCTC-CACTGAAca 3'
 		429 - 449 121.00 -6.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-30d :: TP53    [ Functional MTI ]
Validation Method Luciferase reporter assay , qRT-PCR , Western blot
Conditions 293T, H1299, HCT116, HCT116p53, H929, MM1.s
Location of target site 3'UTR
Tools used in this research miRanda, PicTar, RNA22, TargetScan
Original Description (Extracted from the article) ... miRNAs (miR-25 and miR-30d) directly target the 3'UTR of TP53 to downregulate p53 protein levels and reduce the expression of genes that are transcriptionally activated by p53 ...

- Kumar, M. Lu, Z. Takwi, A. A. Chen, W. et al., 2011, Oncogene.
miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1 
miRNA  3' gaAGGUCAG-CCCCUA-CAAAUGu 5'
            | ||||:  ||| | |||||| 
Target 5' --UGCAGUUAAGGGUUAGUUUACa 3'
 		1 - 22
Article - Kumar, M. Lu, Z. Takwi, A. A. Chen, W. et al.
- Oncogene, 2011
The tumor suppressor p53, encoded by the TP53 gene, is recognized as the guardian of the human genome because it regulates many downstream genes to exercise its function in cell cycle and cell death. Recent studies have revealed that several microRNAs (miRNAs) are important components of the p53 tumor suppressor network with miR-125b and miR-504 directly targeting TP53. In this study, we use a screening method to identify that two miRNAs (miR-25 and miR-30d) directly target the 3'UTR of TP53 to downregulate p53 protein levels and reduce the expression of genes that are transcriptionally activated by p53. Correspondingly, both miR-25 and miR-30d adversely affect apoptotic cell death, cell cycle arrest and cellular senescence. Inhibition of either miR-25 or miR-30d expression increases endogenous p53 expression and elevates cellular apoptosis in several cell lines, including one from multiple myeloma that has little TP53 mutations. Thus, beyond miR-125b and miR-504, the human TP53 gene is negatively regulated by two more miRNAs: miR-25 and miR-30d.
LinkOut: [PMID: 20935678]