Accession ID: MIRT005427 [miRNA, hsa-miR-214 :: MAPK8, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-214 LinkOut: [miRBase ]
Synonyms MIRN214, miRNA214, mir-214, MIR214
Description Homo sapiens miR-214 stem-loop
Comment This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-214-3p
Mature Sequence 71| ACAGCAGGCACAGACAGGCAGU |92
Evidence Experimental
Experiments Cloned
Putative hsa-miR-214-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-214-5p
Mature Sequence 30| UGCCUGUCUACACUUGCUGUGC |51
Evidence Experimental
Experiments Cloned
Putative hsa-miR-214-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol MAPK8 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms JNK, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1
Description mitogen-activated protein kinase 8
Transcript NM_002750   LinkOut: [ RefSeq ]
Other Transcripts NM_139046, NM_139047, NM_139049   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04010    MAPK signaling pathway - Homo sapiens (human)
hsa04012    ErbB signaling pathway - Homo sapiens (human)
hsa04310    Wnt signaling pathway - Homo sapiens (human)
hsa04510    Focal adhesion - Homo sapiens (human)
hsa04620    Toll-like receptor signaling pathway - Homo sapiens (human)
hsa04621    NOD-like receptor signaling pathway - Homo sapiens (human)
hsa04622    RIG-I-like receptor signaling pathway - Homo sapiens (human)
hsa04664    Fc epsilon RI signaling pathway - Homo sapiens (human)
hsa04722    Neurotrophin signaling pathway - Homo sapiens (human)
hsa04910    Insulin signaling pathway - Homo sapiens (human)
hsa04912    GnRH signaling pathway - Homo sapiens (human)
hsa04914    Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa04920    Adipocytokine signaling pathway - Homo sapiens (human)
hsa04930    Type II diabetes mellitus - Homo sapiens (human)
hsa05120    Epithelial cell signaling in Helicobacter pylori infection - Homo sapiens (human)
hsa05142    Chagas disease - Homo sapiens (human)
hsa05200    Pathways in cancer - Homo sapiens (human)
hsa05210    Colorectal cancer - Homo sapiens (human)
hsa05212    Pancreatic cancer - Homo sapiens (human)
Putative miRNA Targets on MAPK8 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of MAPK8
(miRNA target sites are highlighted)		 
>MAPK8|NM_002750|3'UTR
   1 TGATCAATGGCTCTCAGCATCCATCATCATCGTCGTCTGTCAATGATGTGTCTTCAATGTCAACAGATCCGACTTTGGCC
  81 TCTGATACAGACAGCAGTCTAGAAGCAGCAGCTGGGCCTCTGGGCTGCTGTAGATGACTACTTGGGCCATCGGGGGGTGG
 161 GAGGGATGGGGAGTCGGTTAGTCATTGATAGAACTACTTTGAAAACAATTCAGTGGTCTTATTTTTGGGTGATTTTTCAA
 241 AAAATGTA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' ugaCGGACAGACACGGACGACa 5'
             |||  |||| | |||||| 
Target 5' tggGCC--TCTG-GGCTGCTGt 3'
 		113 - 131 130.00 -20.10
2 
miRNA  3' ugacGGACAGACA-CGGACGACa 5'
              :| | || | |||| ||| 
Target 5' cagaTCCGACTTTGGCCT-CTGa 3'
 		64 - 85 102.00 -12.60
3 
miRNA  3' ugaCGGACAG-----ACACGGACGaca 5'
             |:|||||     ||||:|| |   
Target 5' gtcGTCTGTCAATGATGTGTCTTCaat 3'
 		32 - 58 87.00 -14.32
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-214 :: MAPK8    [ Functional MTI ]
Validation Method GFP reporter assay , Microarray , qRT-PCR , Western blot
Conditions HeLa
Location of target site 3'UTR
Tools used in this research miRBase Target Database, PicTar, TargetScan
Original Description (Extracted from the article) ... miR-214 negatively regulates MEK3 and JNK1 through binding to their 3'UTRs by assay of GFP expression (EGFP).// ...

- Yang, Z. Chen, S. Luan, X. Li, Y. Liu, M. et al., 2009, IUBMB Life.
miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1 
miRNA  3' ugaCGGACAGACACGGACGACa 5'
             |||  |||| | |||||| 
Target 5' tggGCC--TCTG-GGCTGCTGt 3'
 		3 - 21
Article - Yang, Z. Chen, S. Luan, X. Li, Y. Liu, M. et al.
- IUBMB Life, 2009
MicroRNAs are a group of endogenously expressed, single-stranded, 18-24 nt RNAs that regulate diverse cellular pathways. Although documented evidence indicates that some microRNAs can function as oncogenes or tumor-suppressors, the role of miR-214 in regulating human cervical cancer cells remains unexplored. We determined the expression level of miR-214 and found it is downregulated in cervical cancer compared with normal tissue. Overexpression of miR-214 in HeLa cells, a human cervical cancer cell line, significantly inhibited cell proliferation according to the MTT and colony forming assays. HeLa cells that stably overexpress miR-214 downregulate the expression of MEK3 and JNK1 at both mRNA and protein levels. Further investigation revealed that miR-214 regulates the expression of MEK3 and JNK1 by targeting the 3'UTRs of these genes. Collectively, these results suggest that miR-214 negatively regulates HeLa cell proliferation by targeting the noncoding regions of MEK3 and JNK1 mRNAs.
LinkOut: [PMID: 19859982]