Accession ID: MIRT005456 [miRNA, hsa-miR-31-5p :: DMD, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-31LinkOut: [miRBase ]
Synonyms MIRN31, hsa-mir-31, miR-31, MIR31
Description Homo sapiens miR-31 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations.
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-31-5p
Mature Sequence 8| AGGCAAGAUGCUGGCAUAGCU |28
Evidence Experimental
Experiments Cloned
Putative hsa-miR-31-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol DMD LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272
Description dystrophin
Transcript NM_0001    LinkOut: [ RefSeq ]
Other Transcripts NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040 , NM_0040   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on DMD LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of DMD
(miRNA target sites are highlighted)
>DMD|NM_0001|3'UTR
   1 TAGGAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGA
  81 GCAGAATAAATGTTTTACAACTCCTGATTCCCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGA
 161 GTTTACAAGAAATAAATCTATATTTTTGTGAAGGGTAGTGGTATTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTAT
 241 TGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTTATAAGAAAACTACATGTAAAATCTTG
 321 ATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAGAAAGAT
 401 TGTAAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACAT
 481 ACACACACACACACAAAACTTTGAGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTT
 561 TTTCTTTTTTTGCATATTAAAGATAAGACTTCCTCTACCACCACACCAAATGACTACTACACACTGCTCATTTGAGAACT
 641 GTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATAAGTATATAAATACTATAGTTATATAG
 721 ATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGAAATTAC
 801 TTCTAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACC
 881 ACACTAAAACATTGTCTACAGCTCCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTAT
 961 TGGATTTTTTTAAAGGGAACATGTGAATGAATACACAGGACTTATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATT
1041 GATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGATTTAATGATGCTTCAGTGGAAATCAA
1121 TCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGGGCTGGA
1201 GGGCTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACA
1281 TAAAGTTCTCTGTAATTACAACTAAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTG
1361 CTTTTTTAGATTTATTGTCCCATGTGGGATGAGTTTTTAAATGCCACAAGACATAATTTAAAATAAATAAACTTTGGGAA
1441 AAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGGTATCAACCCAGAAGCCCATGAACTGTGTTTCCATCC
1521 TTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTTACAAAA
1601 AAACCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCA
1681 AGAGGAAAAGCTAAGGACTGGTAGGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCA
1761 TTCAATAGATACCACCGTGTGACCTATAATTTTGCAAATCTGTTACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGT
1841 GGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAATAATTAATTAATTAACATCAAACACG
1921 GCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCTTCACCA
2001 CTTGTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTC
2081 TAAGGTAACAAATTACCAAATTACATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTT
2161 CTTTACCCAAGGATTTTTAAAACTCAGATTTAAAACAAGGGGTTACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGT
2241 TTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAGACACATTAGC
2321 TCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTTGGACAT
2401 TATTTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGC
2481 ATGTTTTGTCATTGTTTTCAGGTACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACA
2561 TTTACGAATTATTTTTTTAAACTTCAGTTTTACTGCATTTTCACAACATATCAGACTTCACCAAATATATGCCTTACTAT
2641 TGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' ucgaUACGGUCGUAGAACGGa 5'
              ||| || ::|:|||:| 
Target 5' tcacATGGCA-TGTTTTGTCa 3'
2472 - 2491 124.00 -7.60
2
miRNA  3' ucgauaCGGUCGUAGAACGga 5'
                |::  ::||||||  
Target 5' gatttgGTTTTTGTCTTGCat 3'
2110 - 2130 123.00 -7.80
3
miRNA  3' ucGAUACGGUCG-----UAGAACGGa 5'
            || || :|||     | |||||| 
Target 5' atCT-TGATAGCTAAATAACTTGCCa 3'
315 - 339 122.00 -10.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-31-5p :: DMD    [ Functional MTI ]
Validation Method Luciferase reporter assay , qRT-PCR , Western blot , Other
Conditions LA3�mHC2
Location of target site 3'UTR
Tools used in this research unknown
Original Description (Extracted from the article) ... The intense and localized expression of miR-31 in regenerating myoblasts of dystrophic muscles indicates that the high levels of miR-31 found in dystrophic conditions are due to the intensive regeneration programme that is mediated by the activation of satellite cells. Interestingly, in dystrophic myoblasts and satellite cells the lack of dystrophin correlates with a delay of the maturation process of the cells. ...

- Cacchiarelli, D. Incitti, T. Martone, J. et al., 2011, EMBO reports.

Article - Cacchiarelli, D. Incitti, T. Martone, J. et al.
- EMBO reports, 2011
Duchenne muscular dystrophy (DMD)-which is caused by mutations in the dystrophin gene-is one of the most severe myopathies. Among therapeutic strategies, exon skipping allows the rescue of dystrophin synthesis through the production of a shorter but functional messenger RNA. Here, we report the identification of a microRNA-miR-31-that represses dystrophin expression by targeting its 3' untranslated region. In human DMD myoblasts treated with exon skipping, we demonstrate that miR-31 inhibition increases dystrophin rescue. These results indicate that interfering with miR-31 activity can provide an ameliorating strategy for those DMD therapies that are aimed at efficiently recovering dystrophin synthesis.
LinkOut: [PMID: 21212803]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
88 hsa-miR-31-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000088 RHOA ras homolog gene family, member A 4 3
MIRT000490 PPP2R2A protein phosphatase 2, regulatory subunit B, alpha 3 1
MIRT000491 LATS2 LATS, large tumor suppressor, homolog 2 (Drosophila) 3 1
MIRT001180 FOXP3 forkhead box P3 4 1
MIRT004019 SELE selectin E 3 1
MIRT004597 CASR calcium-sensing receptor 1 1
MIRT004968 YY1 YY1 transcription factor 3 1
MIRT004969 RET ret proto-oncogene 3 1
MIRT004970 NUMB numb homolog (Drosophila) 3 1
MIRT004971 NFAT5 nuclear factor of activated T-cells 5, tonicity-responsive 3 1
MIRT004972 KLF13 Kruppel-like factor 13 3 1
MIRT004973 JAZF1 JAZF zinc finger 1 3 1
MIRT004974 HOXC13 homeobox C13 3 1
MIRT004975 ETS1 v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) 3 1
MIRT004976 ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) 4 1
MIRT004977 MPRIP myosin phosphatase Rho interacting protein 3 1
MIRT004978 MMP16 matrix metallopeptidase 16 (membrane-inserted) 4 1
MIRT004979 RDX radixin 4 1
MIRT004980 CXCL12 chemokine (C-X-C motif) ligand 12 3 1
MIRT004981 ARPC5 actin related protein 2/3 complex, subunit 5, 16kDa 3 1
MIRT004984 FZD3 frizzled homolog 3 (Drosophila) 4 1
MIRT005456 DMD dystrophin 4 1
MIRT005566 TIAM1 T-cell lymphoma invasion and metastasis 1 4 1
MIRT005707 ICAM1 intercellular adhesion molecule 1 1 1
MIRT005874 DKK1 dickkopf homolog 1 (Xenopus laevis) 4 1
MIRT005875 DACT3 dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) 4 1
MIRT006026 WASF3 WAS protein family, member 3 2 1
MIRT006468 HIF1AN hypoxia inducible factor 1, alpha subunit inhibitor 3 2
MIRT006567 SATB2 SATB homeobox 2 1 1
MIRT007286 PRKCE protein kinase C, epsilon 1 1
MIRT007292 RASA1 RAS p21 protein activator (GTPase activating protein) 1 3 1
MIRT007319 STK40 serine/threonine kinase 40 2 2
MIRT007327 MCM2 minichromosome maintenance complex component 2 1 1
MIRT007328 CDK1 cyclin-dependent kinase 1 1 1
MIRT007367 CREG1 cellular repressor of E1A-stimulated genes 1 3 1
MIRT028387 Lats2 large tumor suppressor 2 1 1
MIRT035519 MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) 1 1
MIRT035528 MET met proto-oncogene (hepatocyte growth factor receptor) 1 2
MIRT049857 PPIL2 peptidylprolyl isomerase (cyclophilin)-like 2 1 1
MIRT049858 ZC3H18 zinc finger CCCH-type containing 18 1 1
MIRT049859 RPS7 ribosomal protein S7 1 1
MIRT049860 MED12 mediator complex subunit 12 1 1
MIRT049861 CYP27B1 cytochrome P450, family 27, subfamily B, polypeptide 1 1 1
MIRT049862 HIST1H2BC histone cluster 1, H2bc 1 1
MIRT049863 PTPRJ protein tyrosine phosphatase, receptor type, J 1 1
MIRT049864 AGO1 eukaryotic translation initiation factor 2C, 1 1 1
MIRT049865 HEATR2 HEAT repeat containing 2 1 1
MIRT049866 HIST1H2BK histone cluster 1, H2bk 1 1
MIRT049867 RPA1 replication protein A1, 70kDa 1 1
MIRT049868 TXNDC5 thioredoxin domain containing 5 (endoplasmic reticulum) 1 1
MIRT049869 AKAP8L A kinase (PRKA) anchor protein 8-like 1 1
MIRT049870 FLNA filamin A, alpha 1 1
MIRT049871 ATP2A2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 1 1
MIRT049872 NDFIP2 Nedd4 family interacting protein 2 1 1
MIRT049873 TRIB3 tribbles homolog 3 (Drosophila) 1 1
MIRT049874 NUP188 nucleoporin 188kDa 1 1
MIRT049875 RPS4Y1 ribosomal protein S4, Y-linked 1 1 1
MIRT049876 GLI2 GLI family zinc finger 2 1 1
MIRT049877 RPL37A ribosomal protein L37a 1 1
MIRT049878 EXOSC5 exosome component 5 1 1
MIRT049879 DDX19A DEAD (Asp-Glu-Ala-As) box polypeptide 19A 1 1
MIRT049880 UBA6 ubiquitin-like modifier activating enzyme 6 1 1
MIRT049881 NOL9 nucleolar protein 9 1 1
MIRT049882 DNAJC5 DnaJ (Hsp40) homolog, subfamily C, member 5 1 1
MIRT049883 FAM134C family with sequence similarity 134, member C 1 1
MIRT049884 PEX19 peroxisomal biogenesis factor 19 1 1
MIRT049885 SMEK2 SMEK homolog 2, suppressor of mek1 (Dictyostelium) 1 1
MIRT049886 TRRAP transformation/transcription domain-associated protein 1 1
MIRT049887 RANGAP1 Ran GTPase activating protein 1 1 1
MIRT049888 TNRC6B trinucleotide repeat containing 6B 1 1
MIRT049889 NOP56 NOP56 ribonucleoprotein homolog (yeast) 1 1
MIRT049890 PCSK1N proprotein convertase subtilisin/kexin type 1 inhibitor 1 1
MIRT049891 ZNF275 zinc finger protein 275 1 1
MIRT049892 C2CD5 KIAA0528 1 1
MIRT049893 NFATC2IP nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein 1 1
MIRT049894 HIST1H2BJ histone cluster 1, H2bj 1 1
MIRT049895 SRRM2 serine/arginine repetitive matrix 2 1 1
MIRT049896 GYG1 glycogenin 1 1 1
MIRT049897 ZC3H12C zinc finger CCCH-type containing 12C 1 1
MIRT049898 LRRC59 leucine rich repeat containing 59 1 1
MIRT049899 GHITM growth hormone inducible transmembrane protein 1 1
MIRT049900 EDC3 enhancer of mRNA decapping 3 homolog (S. cerevisiae) 1 1
MIRT049901 MYO1D myosin ID 1 1
MIRT049902 STOML2 stomatin (EPB72)-like 2 1 1
MIRT049903 EXOC6 exocyst complex component 6 1 1
MIRT049904 HOXA7 homeobox A7 1 1
MIRT049905 SFXN1 sideroflexin 1 1 1
MIRT049906 ILF2 interleukin enhancer binding factor 2, 45kDa 1 1