Accession ID: MIRT005488 [miRNA, hsa-miR-138 :: CEBPA, target gene]
|Description||CCAAT/enhancer binding protein (C/EBP), alpha|
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05221 Acute myeloid leukemia - Homo sapiens (human)
|Putative miRNA Targets on CEBPA|
|3'UTR of CEBPA
(miRNA target sites are highlighted)
>CEBPA|NM_004364|3'UTR 1 TGAGGCGCGCGGCTGTGGGACCGCCCTGGGCCAGCCTCCGGCGGGGACCCAGGGAGTGGTTTGGGGTCGCCGGATCTCGA 81 GGCTTGCCCGAGCCGTGCGAGCCAGGACTAGGAGATTCCGGTGCCTCCTGAAAGCCTGGCCTGCTCCGCGTGTCCCCTCC 161 CTTCCTCTGCGCCGGACTTGGTGCGTCTAAGATGAGGGGGCCAGGCGGTGGCTTCTCCCTGCGAGGAGGGGAGAATTCTT 241 GGGGCTGAGCTGGGAGCCCGGCAACTCTAGTATTTAGGATAACCTTGTGCCTTGGAAATGCAAACTCACCGCTCCAATGC 321 CTACTGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTGCCTCCTTCCCGAGGCTACAGCAGACC 401 CCCATGAGAGAAGGAGGGGAGCAGGCCCGTGGCAGGAGGAGGGCTCAGGGAGCTGAGATCCCGACAAGCCCGCCAGCCCC 481 AGCCGCTCCTCCACGCCTGTCCTTAGAAAGGGGTGGAAACATAGGGACTTGGGGCTTGGAACCTAAGGTTGTTCCCCTAG 561 TTCTACATGAAGGTGGAGGGTCTCTAGTTCCACGCCTCTCCCACCTCCCTCCGCACACACCCCACCCCAGCCTGCTATAG 641 GCTGGGCTTCCCCTTGGGGCGGAACTCACTGCGATGGGGGTCACCAGGTGACCAGTGGGAGCCCCCACCCCGAGTCACAC 721 CAGAAAGCTAGGTCGTGGGTCAGCTCTGAGGATGTATACCCCTGGTGGGAGAGGGAGACCTAGAGATCTGGCTGTGGGGC 801 GGGCATGGGGGGTGAAGGGCCACTGGGACCCTCAGCCTTGTTTGTACTGTATGCCTTCAGCATTGCCTAGGAACACGAAG 881 CACGATCAGTCCATCCCAGAGGGACCGGAGTTATGACAAGCTTTCCAAATATTTTGCTTTATCAGCCGATATCAACACTT 961 GTATCTGGCCTCTGTGCCCCAGCAGTGCCTTGTGCAATGTGAATGTGCGCGTCTCTGCTAAACCACCATTTTATTTGGTT 1041 TTTGTTTTGTTTTGGTTTTGCTCGGATACTTGCCAAAATGAGACTCTCCGTCGGCAGCTGGGGGAAGGGTCTGAGACTCC 1121 CTTTCCTTTTGGTTTTGGGATTACTTTTGATCCTGGGGGACCAATGAGGTGAGGGGGGTTCTCCTTTGCCCTCAGCTTTC 1201 CCCAGCCCCTCCGGCCTGGGCTGCCCACAAGGCTTGTCCCCCAGAGGCCCTGGCTCCTGGTCGGGAAGGGAGGTGGCCTC 1281 CCGCCAACGCATCACTGGGGCTGGGAGCAGGGAAGGACGGCTTGGTTCTCTTCTTTTGGGGAGAACGTAGAGTCTCACTC 1361 TAGATGTTTTATGTATTATATCTATAATATAAACATATCAAAGTCAA
|miRNA:Target||hsa-miR-138 :: CEBPA [ Functional MTI ]|
|Conditions||adipose tissue-derived mesenchymal stem cells (hAD-MSCs)|
|Original Description (Extracted from the article)||... key adipogenic transcription factors and adipocyte-speciﬁc genes were down-regulated at day 3 of differentiation due to the overexpression of miR-138 (Fig. 1E). Therefore, over- expression of miR-138 resulted in adipogenesis inhibition of hAD-MSCs.// ...
- Yang, Z. Bian, C. Zhou, H. Huang, S. Wang, et al., 2011, Stem cells and development.
MicroRNA hsa-miR-138 Inhibits Adipogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells Through Adenovirus EID-1- Yang, Z. Bian, C. Zhou, H. Huang, S. Wang, et al.
- Stem cells and development, 2011
A better understanding of the molecular mechanisms underlying the differentiation of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) could provide new insights into the pathogenesis of a number of diseases, such as obesity and diabetes, and broaden the spectrum of potential hAD-MSCs-based cell therapy. In this study, we reported that a human microRNA, hsa-miR-138, could inhibit the adipogenic differentiation of hAD-MSCs. Our results showed that miR-138 was significantly down-regulated during adipogenic differentiation. Overexpression of miR-138 in hAD-MSCs could effectively reduce lipid droplets accumulation, inhibit expression of key adipogenic transcription factors cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT) enhancer binding protein alpha and peroxisome proliferator-activated receptor gamma 2 as well as several other adipogenic marker genes, such as fatty acid binding protein 4 and lipoprotein lipase. Further studies showed that the expression of adenovirus early region 1-A-like inhibitor of differentiation 1 (EID-1), a nuclear receptor coregulator, was inversely correlated with that of miR-138 when hAD-MSCs were differentiated into adipocytes. Knockdown of EID-1 by RNA interference inhibited adipocyte differentiation of hAD-MSCs. In addition, luciferase reporter assays demonstrated that miR-138 directly targeted the 3' untranslated region of EID-1, implying that the negative role of miR-138 in the adipocyte differentiation of hAD-MSCs is at least partially mediated via repressing EID-1. Taken together, this study shows that miR-138 plays a negative role in adipogenic differentiation and sheds light on the role of miRNAs during differentiation of hAD-MSCs toward adipocytes.LinkOut: [PMID: 20486779]